FastQCFastQC Report
Thu 26 May 2016
SRR1512898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036439
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT165020.5434655529058875No Hit
TATCAACGCAGAGTACTTTTTTTTT121750.4009631018439692No Hit
GGTATCAACGCAGAGTACTTTTTTT96510.3178394165007102No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58840.19377962145789857No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30810.10146754141940609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA602.5478457E-514.2630026
GGTATCA28350.014.0873791
TAGGACC6350.012.1293684
TATACCG803.740672E-410.6974285
TCCAACG1701.4006218E-1010.61000918
CTAGGAC3500.010.3247183
GTATCAA60250.010.2428691
TGGCGAG14450.010.05158718
CGTGCGC1901.0004442E-109.99692210
AATACCG1054.0660147E-59.9616275
CCTACAC3450.09.9230713
GTCCTAC13300.09.8664621
GCCCCGT1451.6337981E-79.82132414
GTTCTAA2950.09.6701521
GTAGGAC13450.09.61566453
AGGACCT11700.09.5088255
TATACTG4400.09.5088255
CCGTATA800.00452410769.4983279
TTAGGAC9250.09.4582063
TGTAGGA15500.09.3869052
Produced by FastQC (version 0.11.5)