##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512898_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3036439 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.152744382482243 31.0 30.0 34.0 26.0 34.0 2 30.17099240261372 31.0 30.0 34.0 26.0 34.0 3 30.332414384086096 31.0 30.0 34.0 26.0 34.0 4 33.912114157406094 37.0 35.0 37.0 30.0 37.0 5 33.50976291636354 37.0 35.0 37.0 28.0 37.0 6 33.384395668742236 37.0 35.0 37.0 27.0 37.0 7 33.21064312505537 37.0 35.0 37.0 26.0 37.0 8 33.144208396743686 37.0 35.0 37.0 26.0 37.0 9 34.4724916258815 39.0 35.0 39.0 26.0 39.0 10 34.377631165980944 39.0 35.0 39.0 26.0 39.0 11 34.37182403466692 39.0 35.0 39.0 26.0 39.0 12 34.243755925938245 39.0 35.0 39.0 25.0 39.0 13 34.21342039145196 39.0 35.0 39.0 25.0 39.0 14 35.276545980340785 40.0 35.0 41.0 25.0 41.0 15 35.25518707933866 40.0 35.0 41.0 25.0 41.0 16 35.2445232721619 40.0 35.0 41.0 25.0 41.0 17 35.23202837270895 40.0 35.0 41.0 25.0 41.0 18 35.16411526791745 40.0 35.0 41.0 25.0 41.0 19 35.17674321796025 40.0 35.0 41.0 25.0 41.0 20 35.15552658887598 40.0 35.0 41.0 24.0 41.0 21 35.12671257351128 40.0 35.0 41.0 24.0 41.0 22 35.07990610053421 40.0 35.0 41.0 24.0 41.0 23 34.93111569176921 40.0 35.0 41.0 23.0 41.0 24 34.884776542522346 40.0 35.0 41.0 23.0 41.0 25 34.81198733121265 40.0 35.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7317730202233435 1101 2 0.629319389256306 1101 3 0.642123396678798 1101 4 0.6129681310830861 1101 5 0.5676837058788564 1101 6 0.7000606185483704 1101 7 0.715201252231509 1101 8 0.507940875227554 1101 9 0.7854873870402841 1101 10 0.5737410877109426 1101 11 0.793099935136766 1101 12 0.8400761169158173 1101 13 1.0110528165738728 1101 14 1.0586133453945692 1101 15 0.7757945103591339 1101 16 0.9756096909894652 1101 17 1.0004035249659466 1101 18 0.9486630838186372 1101 19 0.8242898541766763 1101 20 0.9122070912275575 1101 21 0.9966884651539516 1101 22 0.9844127817070998 1101 23 1.0722396241354843 1101 24 1.152529621984776 1101 25 1.2063168062719711 1102 1 0.8636122295067352 1102 2 0.9930753834874899 1102 3 0.6072293355861937 1102 4 0.6953873469841412 1102 5 0.8838328715791164 1102 6 0.7358928803503844 1102 7 0.7426535190940982 1102 8 0.757852610940752 1102 9 0.8938106415324043 1102 10 0.8718673800056322 1102 11 1.0168935313092007 1102 12 0.9851226528700323 1102 13 0.9517577761850049 1102 14 0.9962048895650284 1102 15 1.0666538123637608 1102 16 1.068559889564611 1102 17 1.1690903535855668 1102 18 0.6217317138082521 1102 19 0.9679129924859708 1102 20 1.0716593689306961 1102 21 0.9763434957173374 1102 22 0.7533387266769935 1102 23 0.9409948385858726 1102 24 0.9764084314849413 1102 25 1.0620301356944921 1103 1 1.0166149775909936 1103 2 1.1760491674451714 1103 3 0.8296737074254459 1103 4 0.7900196228428982 1103 5 0.9133896703315543 1103 6 0.9451884607654293 1103 7 1.0216906272515587 1103 8 1.1084258200256656 1103 9 1.1277140236203422 1103 10 1.1008070123516447 1103 11 1.2382414756579863 1103 12 1.3777096003718015 1103 13 1.3705521870105741 1103 14 1.4358049240413564 1103 15 1.479274314154729 1103 16 1.4822488368939943 1103 17 1.4221298630595456 1103 18 1.421596414255042 1103 19 1.5373838810332145 1103 20 1.3267604427080357 1103 21 1.5129216798169338 1103 22 1.4094533644851026 1103 23 1.5970147867511102 1103 24 1.4944373074162556 1103 25 1.5606803472629025 1104 1 1.043133314760265 1104 2 1.1839919586296013 1104 3 1.0342173308433118 1104 4 0.9305125580370301 1104 5 1.0578424484169346 1104 6 1.026828944302828 1104 7 0.9517074375288743 1104 8 0.9754916235319797 1104 9 1.0634507178119676 1104 10 1.1265141862996018 1104 11 1.218632771027032 1104 12 1.2726627460884714 1104 13 1.1479645615694878 1104 14 1.3958921579077312 1104 15 1.4681998032240529 1104 16 1.4227371430266018 1104 17 1.4393619037473968 1104 18 0.9868782532694524 1104 19 1.4191955201300317 1104 20 1.2384917815543446 1104 21 1.2963907424219983 1104 22 1.2330202758734927 1104 23 1.185355377934151 1104 24 1.490234558511915 1104 25 1.322858719768675 1105 1 0.7508844061747908 1105 2 0.7838877338087045 1105 3 0.8056003649889121 1105 4 0.6666652629463101 1105 5 0.594361168385646 1105 6 0.7695300197742512 1105 7 0.7166876956003492 1105 8 0.667885333725728 1105 9 0.8858848306704701 1105 10 0.6593419344169433 1105 11 0.9327551953921329 1105 12 0.9710859181391811 1105 13 1.0134648710257892 1105 14 1.0631087100032701 1105 15 1.1616130635005177 1105 16 1.0606706247683704 1105 17 0.7340918263607819 1105 18 0.7379760807944749 1105 19 1.1442123066381384 1105 20 1.1676424565359866 1105 21 0.9255393885642675 1105 22 0.948783728127907 1105 23 1.1027133657856751 1105 24 1.011897238237161 1105 25 0.9464960742927317 1106 1 0.17820606556096408 1106 2 0.486619129774688 1106 3 -1.4777242585120618 1106 4 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1.0331578924310705 1107 17 1.0053894439683475 1107 18 0.9978612070537238 1107 19 0.9799796317574163 1107 20 0.9480829670603441 1107 21 0.9172518867945243 1107 22 0.9228896905210107 1107 23 1.1585401059526177 1107 24 1.0551558828719294 1107 25 1.0816788745489703 1108 1 0.5553206199096614 1108 2 0.5282221464472556 1108 3 0.5185041825835732 1108 4 0.4672145855422869 1108 5 0.5699498059058641 1108 6 0.5641484423851324 1108 7 0.5418148513419183 1108 8 0.4604178238116958 1108 9 0.6514402750415798 1108 10 0.5446694272004109 1108 11 0.5606835413989657 1108 12 0.6338161196819101 1108 13 0.6778866318019396 1108 14 0.7402402307036269 1108 15 0.6195197775011607 1108 16 0.5582811280881472 1108 17 0.6341175466906748 1108 18 0.7953923183554608 1108 19 0.7742864870684727 1108 20 0.8069060319280723 1108 21 0.8664169796847716 1108 22 0.8209196446942784 1108 23 0.9731301820158293 1108 24 0.9369041158625535 1108 25 0.9729798282552053 1109 1 0.42338282760015744 1109 2 0.37855173807877307 1109 3 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91098.0 3 2.0 4 1449.0 5 12608.0 6 15642.0 7 12288.0 8 10075.0 9 8535.0 10 7253.0 11 6483.0 12 5746.0 13 5464.0 14 5333.0 15 5352.0 16 5259.0 17 5460.0 18 6052.0 19 7432.0 20 9966.0 21 13119.0 22 16499.0 23 19971.0 24 22867.0 25 25791.0 26 28965.0 27 31623.0 28 35738.0 29 42731.0 30 53365.0 31 67967.0 32 86377.0 33 110131.0 34 143045.0 35 187616.0 36 258049.0 37 401749.0 38 1269339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.86258259263922 18.840252008493454 13.910238619635804 26.38692677923152 2 14.192791257074008 20.27351460234822 38.821987519236586 26.711706621341175 3 17.648709666581244 24.683699827595408 29.38286138921078 28.284729116612567 4 12.192428476621616 16.558018209992937 35.912088780714335 35.33746453267111 5 13.53805258138949 38.24972454308739 33.983296358698425 14.228926516824695 6 32.9835871958642 35.94383015519106 17.090887641197373 13.98169500774736 7 28.843507057646345 31.81158121072219 20.698770926742068 18.6461408048894 8 26.87258478601785 35.33090429302174 19.38115820868767 18.41535271227274 9 27.074570255766297 14.868229776252667 19.05283161945132 39.00436834852971 10 16.215490429892856 28.722084510327957 32.1241522426134 22.938272817165796 11 35.19261399341552 22.62257643365386 22.851610616857496 19.333198956073126 12 23.847337676815545 24.832047666550192 29.833814545846156 21.486800110788106 13 29.146414162206668 21.37666529435982 25.575331412103747 23.901589131329764 14 22.260780271920428 21.065649059100522 26.73199176789115 29.9415789010879 15 24.085580351143467 29.15938740932219 23.17349928722566 23.581532952308677 16 23.401555248882637 27.603790301597076 25.645072574987566 23.349581874532717 17 22.474806192614473 27.662039705400915 26.645592508156412 23.2175615938282 18 22.510551615224628 26.64770696892399 28.324734501105798 22.517006914745583 19 23.902719365558216 26.202603766998095 27.525927340870844 22.36874952657285 20 24.267609627607495 25.852121250444192 27.636409384424216 22.243859737524097 21 24.144148653958062 25.516981999531684 27.13484065615407 23.20402869035618 22 24.139527745557753 25.994742482415113 27.24760194097379 22.618127831053343 23 23.39921862418445 25.872938662690082 27.523589309714442 23.204253403411034 24 23.566322261043283 25.73267567700191 27.733934388275213 22.967067673679598 25 23.342332919251383 25.83907581124713 27.689699371236543 23.12889189826494 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 216.0 1 216.0 2 592.5 3 969.0 4 969.0 5 969.0 6 2570.0 7 4171.0 8 4171.0 9 4171.0 10 5387.0 11 6603.0 12 6603.0 13 6603.0 14 9043.5 15 11484.0 16 11484.0 17 11484.0 18 20992.0 19 30500.0 20 30500.0 21 30500.0 22 47341.0 23 64182.0 24 64182.0 25 64182.0 26 93908.5 27 123635.0 28 123635.0 29 123635.0 30 154281.0 31 184927.0 32 184927.0 33 184927.0 34 212228.0 35 239529.0 36 239529.0 37 239529.0 38 266104.5 39 292680.0 40 292680.0 41 292680.0 42 318615.5 43 344551.0 44 344551.0 45 344551.0 46 370852.5 47 397154.0 48 397154.0 49 397154.0 50 395896.5 51 394639.0 52 394639.0 53 394639.0 54 364412.0 55 334185.0 56 334185.0 57 334185.0 58 295100.0 59 256015.0 60 256015.0 61 256015.0 62 215561.0 63 175107.0 64 175107.0 65 175107.0 66 136275.5 67 97444.0 68 97444.0 69 97444.0 70 72707.5 71 47971.0 72 47971.0 73 47971.0 74 34080.5 75 20190.0 76 20190.0 77 20190.0 78 13818.5 79 7447.0 80 7447.0 81 7447.0 82 4818.0 83 2189.0 84 2189.0 85 2189.0 86 1361.5 87 534.0 88 534.0 89 534.0 90 317.0 91 100.0 92 100.0 93 100.0 94 58.5 95 17.0 96 17.0 97 17.0 98 8.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08473741774493082 2 0.039783443698358505 3 0.01834385607614709 4 0.006389062978047641 5 0.018903722419584254 6 0.06115716469193025 7 0.0751867565921792 8 0.05829196634610476 9 0.0243047859680369 10 0.05005863776614646 11 0.006948929321484805 12 3.951997718379984E-4 13 0.006224396406448475 14 0.0011855993155139952 15 0.013667325442730778 16 0.00885906155203513 17 0.005829196634610476 18 0.0057303966916509765 19 0.0022723986880684908 20 0.0015149324587123273 21 5.927996577569976E-4 22 8.2333285799583E-4 23 0.0 24 0.0 25 9.87999429594996E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3036439.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.46913021021827 #Duplication Level Percentage of deduplicated Percentage of total 1 69.86905321266119 33.86492237829144 2 15.130715604858263 14.66745249651313 3 6.000969953347082 8.725853821691615 4 2.92635474307905 5.673514763343532 5 1.6458937440290213 3.9887519095763144 6 1.0828281601508367 3.1490243453785185 7 0.6888744635638653 2.337240225107987 8 0.4932337685433446 1.9125289401283225 9 0.35418059153363934 1.5450142688078485 >10 1.6758197596860445 14.135536935063705 >50 0.08900811571894865 2.9378579851421893 >100 0.03829591502545126 3.3997752365896172 >500 0.002931351650921153 0.9080568237895029 >1k 0.0015679322779386163 1.293622469236086 >5k 1.363419372120536E-4 0.513305536501816 >10k+ 1.363419372120536E-4 0.9475418648382734 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 16502 0.5434655529058875 No Hit TATCAACGCAGAGTACTTTTTTTTT 12175 0.4009631018439692 No Hit GGTATCAACGCAGAGTACTTTTTTT 9651 0.3178394165007102 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 5884 0.19377962145789857 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3081 0.10146754141940609 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.58666286396664E-5 2 0.0 0.0 0.0 0.0 6.58666286396664E-5 3 0.0 3.29333143198332E-5 0.0 0.0 6.58666286396664E-5 4 0.0 3.29333143198332E-5 0.0 0.0 9.87999429594996E-5 5 0.0 3.29333143198332E-5 0.0 0.0 9.87999429594996E-5 6 0.0 3.29333143198332E-5 0.0 0.0 9.87999429594996E-5 7 0.0 3.29333143198332E-5 0.0 3.29333143198332E-5 1.317332572793328E-4 8 0.0 3.29333143198332E-5 0.0 6.58666286396664E-5 1.317332572793328E-4 9 0.0 3.29333143198332E-5 0.0 6.58666286396664E-5 1.64666571599166E-4 10 0.0 3.29333143198332E-5 0.0 6.58666286396664E-5 1.64666571599166E-4 11 0.0 3.29333143198332E-5 0.0 6.58666286396664E-5 1.975998859189992E-4 12 0.0 3.29333143198332E-5 0.0 6.58666286396664E-5 2.963998288784988E-4 13 0.0 3.29333143198332E-5 0.0 6.58666286396664E-5 2.963998288784988E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGA 60 2.5478457E-5 14.263002 6 GGTATCA 2835 0.0 14.087379 1 TAGGACC 635 0.0 12.129368 4 TATACCG 80 3.740672E-4 10.697428 5 TCCAACG 170 1.4006218E-10 10.610009 18 CTAGGAC 350 0.0 10.324718 3 GTATCAA 6025 0.0 10.242869 1 TGGCGAG 1445 0.0 10.051587 18 CGTGCGC 190 1.0004442E-10 9.996922 10 AATACCG 105 4.0660147E-5 9.961627 5 CCTACAC 345 0.0 9.923071 3 GTCCTAC 1330 0.0 9.866462 1 GCCCCGT 145 1.6337981E-7 9.821324 14 GTTCTAA 295 0.0 9.670152 1 GTAGGAC 1345 0.0 9.6156645 3 AGGACCT 1170 0.0 9.508825 5 TATACTG 440 0.0 9.508825 5 CCGTATA 80 0.0045241076 9.498327 9 TTAGGAC 925 0.0 9.458206 3 TGTAGGA 1550 0.0 9.386905 2 >>END_MODULE