FastQCFastQC Report
Thu 26 May 2016
SRR1512897_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512897_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091349
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67330.2178013546836672No Hit
GTCCTACAGTGGACATTTCTAAATT46230.14954636309261748No Hit
TATCAACGCAGAGTACTTTTTTTTT39950.1292316073015373No Hit
GTCCTAAAGTGTGTATTTCTCATTT39430.12754949376469626No Hit
GGTATCAACGCAGAGTACTTTTTTT38320.12395882833028558No Hit
CTGTAGGACGTGGAATATGGCAAGA38060.12311777156186507No Hit
CTTTAGGACGTGAAATATGGCGAGG31730.10264127408455015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2100.013.57038718
GGTATCA22300.013.1059071
TAGGACC10050.011.9087634
GCCGGTT1406.82121E-1011.53445511
TCGAACT3400.010.89604719
TTAGGAC13200.010.8658873
CGTGCGC2100.010.85560710
GTCCTAC25450.010.8478531
GTATCAA49400.010.7918191
TCCTACA28600.010.5614432
GTCGAGG1002.40294E-510.44902919
GTCCTAT4700.010.33013251
GCGTGCG1752.3646862E-1010.3126599
TAGAAAT7100.010.1675764
GTAGGAC25050.010.1622343
GTCGTAC750.002603189810.1545891
TACGCTC750.002651946910.13194
TGTAGGA27150.010.0759232
TTTAGGA15400.010.0537722
GTCCTAA12850.010.0014791
Produced by FastQC (version 0.11.5)