##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512897_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3091349
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.677350244181422	31.0	30.0	34.0	18.0	34.0
2	28.85838900751743	31.0	30.0	34.0	16.0	34.0
3	28.952128342674992	31.0	30.0	34.0	20.0	34.0
4	32.383688156853204	35.0	33.0	37.0	22.0	37.0
5	32.562913472403146	35.0	33.0	37.0	25.0	37.0
6	32.45030017639548	36.0	33.0	37.0	23.0	37.0
7	32.388404867907184	36.0	33.0	37.0	22.0	37.0
8	32.294471119242765	36.0	33.0	37.0	22.0	37.0
9	33.593905120385955	38.0	34.0	39.0	19.0	39.0
10	33.46384054340031	38.0	33.0	39.0	18.0	39.0
11	33.52093277077418	38.0	34.0	39.0	19.0	39.0
12	33.41406389249483	38.0	34.0	39.0	17.0	39.0
13	33.39067183938145	38.0	33.0	39.0	17.0	39.0
14	34.22737613902539	39.0	33.0	41.0	17.0	41.0
15	34.394884563341115	39.0	34.0	41.0	17.0	41.0
16	34.3343148897132	39.0	34.0	41.0	17.0	41.0
17	34.08002881589882	39.0	33.0	41.0	16.0	41.0
18	34.08262832827998	39.0	33.0	41.0	16.0	41.0
19	34.19950190030307	39.0	34.0	41.0	17.0	41.0
20	34.15743159377993	39.0	34.0	41.0	16.0	41.0
21	34.05871449648681	39.0	34.0	41.0	10.0	41.0
22	34.05614086277544	39.0	34.0	41.0	10.0	41.0
23	33.7241353208583	39.0	33.0	41.0	2.0	41.0
24	33.77500340466249	39.0	34.0	41.0	2.0	41.0
25	33.488043892811845	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6939429859536013
1101	2	0.4058214884685931
1101	3	0.7267155410334531
1101	4	0.8597140081446781
1101	5	0.6534664313210143
1101	6	0.6509362710189208
1101	7	0.4284711868033142
1101	8	0.7147423936849151
1101	9	0.7093907529575532
1101	10	0.6921043873326127
1101	11	0.7389803256517453
1101	12	0.5405476942065093
1101	13	0.9729539145451724
1101	14	1.0838638508739677
1101	15	0.9856178566428255
1101	16	0.8204750449827358
1101	17	0.2393247542131789
1101	18	0.6206060732276271
1101	19	0.09312288302076155
1101	20	-0.527704907665111
1101	21	0.5816506202179212
1101	22	0.5767757896928245
1101	23	0.10434277239104972
1101	24	0.6545321228340626
1101	25	0.42374891290855743
1102	1	0.8354900045116374
1102	2	0.7963078849651275
1102	3	0.7741924813968062
1102	4	0.964045430552801
1102	5	0.8092611221676549
1102	6	0.6646570942074703
1102	7	0.6662022178974922
1102	8	0.6786259435073418
1102	9	0.9519837422323292
1102	10	1.001493887998258
1102	11	0.7504701217869325
1102	12	0.8480812367963395
1102	13	0.902686391977241
1102	14	1.0439594809775912
1102	15	0.9659723420019688
1102	16	0.8730792295738823
1102	17	1.0699732335379863
1102	18	1.084382972883212
1102	19	0.8641608656445499
1102	20	1.1315403178897796
1102	21	1.2687914704463097
1102	22	1.0105793160222518
1102	23	0.8249798566290565
1102	24	0.3107975000492118
1102	25	0.7721168021369422
1103	1	0.46581285206648815
1103	2	0.6419440829141898
1103	3	0.7170680909551841
1103	4	0.8397005878814028
1103	5	0.6366776568710719
1103	6	0.16789741500045352
1103	7	0.09386463751405216
1103	8	0.3429468266384603
1103	9	0.4923980392677336
1103	10	0.7270932958059149
1103	11	0.4294952686470026
1103	12	0.5682852056171441
1103	13	0.6886648371264243
1103	14	0.6558036597562307
1103	15	0.6469151780400253
1103	16	0.7078008558455835
1103	17	0.43364984368758286
1103	18	0.7761525895165917
1103	19	0.7741485382042512
1103	20	0.9606954654347035
1103	21	0.7272159725616802
1103	22	0.7093525644984453
1103	23	0.06854490134747948
1103	24	0.16889208240475995
1103	25	0.5941859427242733
1104	1	0.9115792441450061
1104	2	0.8196054954313361
1104	3	0.8293554178453881
1104	4	1.000313722731228
1104	5	0.7376764533124813
1104	6	0.6861008970506148
1104	7	0.8666076063329342
1104	8	0.6412598198770709
1104	9	0.9467776324908996
1104	10	0.9161340524280561
1104	11	0.6344970592906805
1104	12	0.9913703104005549
1104	13	0.9322439784512682
1104	14	1.1943488454876672
1104	15	0.9886645224388957
1104	16	0.962746398407198
1104	17	1.2118261687809877
1104	18	0.9863087172427925
1104	19	0.5788769652194645
1104	20	0.7096723808254239
1104	21	0.7530727050060264
1104	22	0.5079820813458724
1104	23	0.9944126373655919
1104	24	0.8193196925583948
1104	25	0.37030811075636194
1105	1	0.9233502274817695
1105	2	1.0400648622336703
1105	3	1.0843806167406917
1105	4	1.2377317964888803
1105	5	0.9824538549472024
1105	6	1.163240416542152
1105	7	1.21200772808362
1105	8	1.209086790375892
1105	9	0.9843149610752135
1105	10	1.1314785964378018
1105	11	1.2014909855384133
1105	12	1.3773326618089214
1105	13	1.2913929438729994
1105	14	1.3804062915066027
1105	15	1.3726299794644703
1105	16	1.326914974361209
1105	17	1.6541573296276937
1105	18	1.4546453044320131
1105	19	1.1790495489961685
1105	20	1.4136599845756948
1105	21	1.2364532915202702
1105	22	1.1985321510617482
1105	23	1.3692731383787233
1105	24	1.58128446183715
1105	25	1.8166958769305168
1106	1	0.5992561190354699
1106	2	0.7254576750765693
1106	3	0.6113496221043953
1106	4	0.7015958518433791
1106	5	0.6481940135410582
1106	6	0.7664527172775308
1106	7	0.6455069732804759
1106	8	0.6281370077864565
1106	9	0.7701849781818169
1106	10	0.5552964264895266
1106	11	0.42058882540428044
1106	12	0.5884545923329512
1106	13	0.643794938734203
1106	14	0.9274883725141905
1106	15	0.6463492421086983
1106	16	0.7301709361133035
1106	17	0.9383143440149553
1106	18	0.7900005900985931
1106	19	0.8036863319331857
1106	20	0.9030181974472171
1106	21	0.8561999553926327
1106	22	0.7811500400664997
1106	23	1.0685379294533064
1106	24	0.6665340665452177
1106	25	0.9343304124965641
1107	1	0.7870109595783177
1107	2	0.7514575207333358
1107	3	0.7663233743943216
1107	4	0.8768212668072266
1107	5	0.6981984337482032
1107	6	0.7779843616965181
1107	7	0.5349214612010371
1107	8	0.7394119889771744
1107	9	0.6622259219886075
1107	10	0.8296827509302389
1107	11	0.751782653671853
1107	12	0.8351313557927966
1107	13	0.6070083731685969
1107	14	0.888261602990859
1107	15	0.616979596929653
1107	16	0.7742555652953627
1107	17	1.0011870376808716
1107	18	0.8997072609920167
1107	19	0.7307485873787272
1107	20	0.8464193689997188
1107	21	0.6381313550257488
1107	22	0.7217966633298545
1107	23	0.9885204483140697
1107	24	0.7858016142995723
1107	25	1.124468462326135
1108	1	0.6675243791783778
1108	2	0.648826994831218
1108	3	0.6364150283097096
1108	4	0.769395185397336
1108	5	0.7001520370144405
1108	6	0.6445907061590006
1108	7	0.601941341249443
1108	8	0.6430279766308331
1108	9	0.7386318175583284
1108	10	0.7891463491676305
1108	11	0.4739115855149265
1108	12	0.6081372291448375
1108	13	0.6135990219129965
1108	14	0.6875376170907259
1108	15	0.6459404663338688
1108	16	0.3805190407382568
1108	17	0.7128147696496114
1108	18	0.8354356844562787
1108	19	0.6854027739188737
1108	20	0.75400527438822
1108	21	0.8605039646837511
1108	22	0.8280564358255589
1108	23	1.0384573190370148
1108	24	0.9941320509726026
1108	25	1.1703518003777376
1109	1	0.6477267481630342
1109	2	0.7005996053279873
1109	3	0.7773327063356525
1109	4	0.7743220712849279
1109	5	0.681220314085607
1109	6	0.7645699690444232
1109	7	0.6747793635017771
1109	8	0.6356274492185037
1109	9	0.834656070755095
1109	10	0.816430709886582
1109	11	0.824852526018347
1109	12	0.6866123956002497
1109	13	0.8382567623931081
1109	14	0.8913164341524649
1109	15	0.7399594842590886
1109	16	0.5346489803723671
1109	17	0.8744399378047802
1109	18	0.9159245850006883
1109	19	0.8708685819621493
1109	20	0.720130930618339
1109	21	0.7586388384884515
1109	22	0.8334672686611029
1109	23	1.0499479630531212
1109	24	0.9343941059381038
1109	25	0.4999021886522854
1110	1	0.561555932445188
1110	2	0.6024148162222502
1110	3	0.5694342972663584
1110	4	0.45094330134656957
1110	5	0.3601421221003491
1110	6	0.4370244662835958
1110	7	0.39874966251475996
1110	8	0.3961842308615431
1110	9	0.6215617851888169
1110	10	0.5181571036128645
1110	11	0.5046597391976775
1110	12	0.49221297719392254
1110	13	0.44974500165609754
1110	14	0.5241191899544901
1110	15	0.48263551602092747
1110	16	0.49195904386429845
1110	17	0.6369910561906238
1110	18	-0.17879604587384534
1110	19	0.3905621210344066
1110	20	0.45901992375273437
1110	21	0.5482349689595551
1110	22	0.6526578953545936
1110	23	0.6400870041003515
1110	24	0.48592939360196397
1110	25	0.7164186936822858
1111	1	0.28800087368532346
1111	2	0.30962156078815894
1111	3	0.3317319872835256
1111	4	0.25629859989306425
1111	5	0.29674721499451095
1111	6	0.11465405280980434
1111	7	0.09516281429688433
1111	8	0.24513910171507547
1111	9	0.27594467165626924
1111	10	0.22579679625407323
1111	11	-0.06170530671031571
1111	12	-0.2863465012941262
1111	13	0.024090236575752044
1111	14	0.16200572449466222
1111	15	0.24923129985261738
1111	16	0.3046931480970443
1111	17	-0.2592766694431248
1111	18	0.07236231584616348
1111	19	0.05693773307970673
1111	20	0.2880812258778107
1111	21	0.17699061980646036
1111	22	-0.19803040503230562
1111	23	0.2911057378697848
1111	24	0.2765706471244087
1111	25	0.4199320421558923
1112	1	0.43497915169692547
1112	2	0.38998666708838314
1112	3	0.3597320415537695
1112	4	0.562920312707206
1112	5	0.515664982329568
1112	6	0.19545909535541028
1112	7	0.18940624347857948
1112	8	0.32158843220438627
1112	9	0.3587214523259874
1112	10	0.3423103271504786
1112	11	0.326760416946243
1112	12	0.4192220780433189
1112	13	0.3214807988510202
1112	14	0.2936787840669126
1112	15	0.0837614051401232
1112	16	0.2926558948045681
1112	17	0.5716768894412212
1112	18	0.2788309574974832
1112	19	0.42066458383234107
1112	20	0.11037088749314705
1112	21	0.18089222236155678
1112	22	0.09481122857958013
1112	23	0.3551940914430176
1112	24	0.49024911498495527
1112	25	0.42450887315479946
1113	1	-0.003526835224501923
1113	2	0.1815644901376352
1113	3	0.11755176473784346
1113	4	0.20370725432878345
1113	5	0.0767700880411013
1113	6	-0.2893881183701623
1113	7	-0.24409505131197307
1113	8	-0.16974943883526805
1113	9	-0.24131274875458786
1113	10	-0.23030188308074173
1113	11	-0.1554724681598998
1113	12	-0.31776295069350624
1113	13	-0.21104994747496164
1113	14	0.12619594400126033
1113	15	-0.17320861174759017
1113	16	-0.2212269721863862
1113	17	-0.09493063063892038
1113	18	-0.28697444577829856
1113	19	-0.6692181821887146
1113	20	-0.40351952749315956
1113	21	-0.20535614677650216
1113	22	-0.6938633384872048
1113	23	-4.328083361977193
1113	24	-1.847562283131463
1113	25	-0.9941054237877935
1114	1	-0.47674761725338044
1114	2	-0.7378288592337583
1114	3	-0.5609541193054177
1114	4	-0.44361863255971556
1114	5	-0.4099119867715686
1114	6	-1.0855351883098372
1114	7	-0.9456447218805728
1114	8	-0.7354872639581167
1114	9	-0.8945874398308149
1114	10	-0.6336513323297623
1114	11	-0.9091593731467782
1114	12	-1.8023124269793307
1114	13	-0.9957721991875843
1114	14	-2.6852968555038643
1114	15	-1.589762521856592
1114	16	-1.4607144690810472
1114	17	-1.3044654030095728
1114	18	-1.2804492516547725
1114	19	-1.3227136974061153
1114	20	-0.9889594930530876
1114	21	-0.7904646107110338
1114	22	-0.9724980631541769
1114	23	-5.056135866455605
1114	24	-2.416541604932398
1114	25	-2.36131084473735
1115	1	-1.490490344770187
1115	2	-1.300603333478815
1115	3	-0.9653444005476821
1115	4	-1.012208753698534
1115	5	-0.841799465497207
1115	6	-1.095203727205643
1115	7	-1.4790563176115121
1115	8	-2.007809418155553
1115	9	-3.061940524788678
1115	10	-3.8121137900845348
1115	11	-2.6058252767013563
1115	12	-2.7737124103986375
1115	13	-2.0389386364056072
1115	14	-2.7876826299409387
1115	15	-2.367817772643093
1115	16	-2.445251649252697
1115	17	-2.3610666153777835
1115	18	-4.997170244073651
1115	19	-2.895317794002512
1115	20	-3.2272162266196425
1115	21	-2.7256363113717974
1115	22	-2.3619808629815218
1115	23	-2.9750806934517975
1115	24	-2.71241434573583
1115	25	-3.0706937330921704
1116	1	-1.3448956732494501
1116	2	-1.2814450115659142
1116	3	-1.5512338264292929
1116	4	-1.4388101632671777
1116	5	-1.5144630492322975
1116	6	-3.8250961554379934
1116	7	-2.387613473340302
1116	8	-2.8098125421985607
1116	9	-3.7378945889683024
1116	10	-2.9402918716652415
1116	11	-2.3547263745498768
1116	12	-2.2544828884750103
1116	13	-3.5976910060523437
1116	14	-6.642396841490449
1116	15	-4.651874981874521
1116	16	-3.3739085543264338
1116	17	-4.535154746668962
1116	18	-3.2208555000703
1116	19	-2.939630518680506
1116	20	-3.6311127236064813
1116	21	-4.463620089445971
1116	22	-4.112332142214463
1116	23	-3.1273772427279987
1116	24	-4.088284812619069
1116	25	-4.008129084075382
1201	1	0.7497376255066719
1201	2	0.8093134071504196
1201	3	1.0319961288822874
1201	4	0.9395336547017479
1201	5	0.46874237887259085
1201	6	1.0632079157072738
1201	7	1.0803778559401138
1201	8	1.1360925987819783
1201	9	1.5602297552167173
1201	10	1.3331164596122633
1201	11	1.5623173416282938
1201	12	1.3713841145067889
1201	13	1.4488430893813202
1201	14	1.552520575176274
1201	15	1.5680569032753553
1201	16	1.503531667267076
1201	17	1.7762337453521297
1201	18	1.552762045045533
1201	19	1.7711953793477733
1201	20	1.6778142472539983
1201	21	1.8102942938579503
1201	22	1.6701520642338323
1201	23	2.118921294478149
1201	24	1.9224194864065893
1201	25	1.984266007172394
1202	1	0.5547727133692888
1202	2	0.6683747277158574
1202	3	0.6940962370817978
1202	4	0.7768788590702727
1202	5	0.7070587754923281
1202	6	0.8796981204335239
1202	7	0.9851275926229235
1202	8	0.9871437175512483
1202	9	1.160205813618063
1202	10	1.003207334451929
1202	11	0.9557110223927907
1202	12	1.189557996224515
1202	13	1.1429624151349174
1202	14	1.5151868972467
1202	15	1.2231270507231073
1202	16	1.0291445995725041
1202	17	1.3958232874248466
1202	18	1.3577783710254394
1202	19	1.4317743836160588
1202	20	1.297896041881046
1202	21	1.4298909277427896
1202	22	1.378925093659376
1202	23	1.431217230950594
1202	24	1.4222335732051619
1202	25	1.8373662556820562
1203	1	1.0308502274817712
1203	2	1.0904774439330147
1203	3	0.8913863356949392
1203	4	1.1942105546588166
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2211	18	-0.33055793780118137
2211	19	-0.1352693637811413
2211	20	-0.24935387520174146
2211	21	0.053813074740361344
2211	22	0.016326946273714782
2211	23	0.2210214260802772
2211	24	-0.06941861389893944
2211	25	0.25570150105972544
2212	1	0.024694410880652384
2212	2	0.15292263298980302
2212	3	-0.17654753755421382
2212	4	0.010178780698971934
2212	5	-0.26976302832166255
2212	6	-0.3345529316326932
2212	7	-0.12994661126614204
2212	8	-0.029777974290759346
2212	9	0.01787511114090279
2212	10	-0.19301109128162608
2212	11	-0.3532609788987813
2212	12	0.10010171664675482
2212	13	0.14109278206398557
2212	14	0.23490171729024922
2212	15	-0.14172460922811325
2212	16	-0.09180652134395473
2212	17	0.10974969301339854
2212	18	0.2892671232140387
2212	19	0.014111615808680256
2212	20	0.2611009567556337
2212	21	0.37102260862129555
2212	22	0.4459245703906234
2212	23	0.3918487766516989
2212	24	0.3834597090491272
2212	25	0.491842042828047
2213	1	-0.15745279348000452
2213	2	-0.05346804517508019
2213	3	-0.24933628041685552
2213	4	-0.009333423968392651
2213	5	-0.0030777549429501505
2213	6	0.16848816401763855
2213	7	-0.0952243766366081
2213	8	0.062200482420728065
2213	9	-0.3569102671994031
2213	10	0.0606154947236206
2213	11	-0.14496385718783245
2213	12	-0.16585264560627877
2213	13	-0.17311115821995315
2213	14	-0.28574811723593285
2213	15	-0.157248962453437
2213	16	-0.013738559966625985
2213	17	-0.14667734353462691
2213	18	-0.0953665827725203
2213	19	-0.0996960789303003
2213	20	0.03903423004042139
2213	21	-0.23090852476398993
2213	22	-0.36128768727226657
2213	23	-0.13457811441174528
2213	24	0.10249682573873287
2213	25	0.2263798183245811
2214	1	-2.5417672938857514
2214	2	-1.3944531116225392
2214	3	-1.4856702882366832
2214	4	-3.276323633375373
2214	5	-1.6119685935193608
2214	6	-1.346491232527729
2214	7	-1.2047312110817927
2214	8	-1.0753557686990227
2214	9	-0.8610470298750101
2214	10	-0.8734579294546094
2214	11	-0.9037471994791844
2214	12	-0.9101044925400004
2214	13	-1.320528876137061
2214	14	-1.1697759608815304
2214	15	-1.5064329918779151
2214	16	-1.3507056892888656
2214	17	-3.313920221905743
2214	18	-3.1857770183432486
2214	19	-2.0284950714161027
2214	20	-1.814350322111089
2214	21	-1.7850242097366404
2214	22	-2.6442028916732916
2214	23	-1.699216052169291
2214	24	-2.8072242108626995
2214	25	-2.6231419812896952
2215	1	-1.532844729532279
2215	2	-3.2103555836059527
2215	3	-1.7992849703091132
2215	4	-3.354739434856807
2215	5	-1.9109325666865224
2215	6	-1.86496945638444
2215	7	-1.9733065274501485
2215	8	-1.3370311544255706
2215	9	-3.724114821685724
2215	10	-1.9974870939482336
2215	11	-1.5643359286829295
2215	12	-1.5297739478720658
2215	13	-1.613675721952177
2215	14	-1.3544887516438067
2215	15	-1.532812449306853
2215	16	-1.6405258060328691
2215	17	-1.8793466403883556
2215	18	-1.512968074182865
2215	19	-1.6022028493910199
2215	20	-1.535749207003363
2215	21	-2.167965454205458
2215	22	-2.036419853784359
2215	23	-1.9675270636340016
2215	24	-1.8633590059155054
2215	25	-2.7611380402387873
2216	1	-2.8581081058515636
2216	2	-2.97806422273365
2216	3	-4.371946997638393
2216	4	-4.70412277867452
2216	5	-4.505569020869789
2216	6	-3.4962775573140554
2216	7	-5.198278219628801
2216	8	-2.984704059297311
2216	9	-3.7362446794476583
2216	10	-2.6739013055229854
2216	11	-2.932283524265511
2216	12	-3.2600938087793168
2216	13	-2.952083363316543
2216	14	-4.015806943787517
2216	15	-3.2949639747838937
2216	16	-3.679290090998272
2216	17	-3.317125350110871
2216	18	-2.903009924326156
2216	19	-3.587992934663962
2216	20	-4.135167629803394
2216	21	-3.8762101071725397
2216	22	-3.517648404493805
2216	23	-3.7100119923402275
2216	24	-4.049777629666117
2216	25	-4.2188310838538
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	165354.0
3	4.0
4	1476.0
5	8995.0
6	10300.0
7	9473.0
8	8313.0
9	7837.0
10	6949.0
11	6592.0
12	6365.0
13	6211.0
14	6050.0
15	6065.0
16	6502.0
17	7226.0
18	8025.0
19	9552.0
20	12351.0
21	15858.0
22	19815.0
23	23885.0
24	27725.0
25	31314.0
26	34857.0
27	38600.0
28	43247.0
29	51040.0
30	61479.0
31	76337.0
32	95274.0
33	120489.0
34	154682.0
35	200966.0
36	270366.0
37	408450.0
38	1123325.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.77366532958097	18.124235048825426	12.674001542965954	24.42809807862765
2	15.17532122165815	19.528135122089466	41.43794977178995	23.858593884462433
3	19.565590137183424	24.909933342909383	28.236673434606562	27.28780308530063
4	12.41450529783263	15.842446136032542	37.51245963660081	34.23058892953402
5	13.687164483894417	38.29758323258211	34.20708928364026	13.808162999883208
6	32.64053973993936	35.53908436512086	18.196086302706956	13.624289592232822
7	29.489107382595964	31.255578393403276	20.986219486572523	18.26909473742824
8	26.40036890032923	35.733171594027	20.12948427461961	17.736975231024154
9	27.901493327842537	14.80510592118767	19.44013317767448	37.85326757329531
10	16.483985783560307	28.384280324308886	32.69182140281952	22.439912489311283
11	35.21366663536969	22.30460162722708	23.54020636580557	18.941525371597667
12	24.301926063984972	24.262713793490892	30.515759544269876	20.91960059825426
13	29.55545986965854	21.414525334999563	25.39042543892308	23.639589356418814
14	22.011712590840894	21.489055707977812	26.954337861105298	29.544893840075993
15	24.00030173950233	29.818629306222533	22.898564050198832	23.2825049040763
16	23.116551617631202	27.483396645107316	26.641484412612105	22.758567324649373
17	22.376710960346163	27.95197022521809	26.83334353328146	22.837975281154286
18	22.206297740389424	26.88279563381363	28.69246583837054	22.21844078742641
19	24.111217134777164	26.28635002682611	27.040913298724362	22.561519539672368
20	24.6229891540678	26.155834711321802	27.37039650127459	21.850779633335804
21	24.50903932250162	25.792573585423646	26.349666369149077	23.348720722925655
22	24.358618992416243	26.943312889792693	26.50978893717335	22.18827918061771
23	23.526836668118335	26.6106614903162	26.970070311374318	22.89243153019115
24	23.859585844356037	26.05598939825789	27.430615476717385	22.65380928066869
25	23.56655211645916	26.392523219242882	27.291926897670855	22.748997766627102
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	314.0
1	314.0
2	773.0
3	1232.0
4	1232.0
5	1232.0
6	2048.5
7	2865.0
8	2865.0
9	2865.0
10	3413.5
11	3962.0
12	3962.0
13	3962.0
14	6203.0
15	8444.0
16	8444.0
17	8444.0
18	17080.5
19	25717.0
20	25717.0
21	25717.0
22	42898.0
23	60079.0
24	60079.0
25	60079.0
26	91084.5
27	122090.0
28	122090.0
29	122090.0
30	160665.5
31	199241.0
32	199241.0
33	199241.0
34	230856.0
35	262471.0
36	262471.0
37	262471.0
38	290312.5
39	318154.0
40	318154.0
41	318154.0
42	341505.0
43	364856.0
44	364856.0
45	364856.0
46	388957.0
47	413058.0
48	413058.0
49	413058.0
50	407813.0
51	402568.0
52	402568.0
53	402568.0
54	365335.5
55	328103.0
56	328103.0
57	328103.0
58	287194.5
59	246286.0
60	246286.0
61	246286.0
62	205952.0
63	165618.0
64	165618.0
65	165618.0
66	128267.0
67	90916.0
68	90916.0
69	90916.0
70	68178.5
71	45441.0
72	45441.0
73	45441.0
74	32299.0
75	19157.0
76	19157.0
77	19157.0
78	13284.0
79	7411.0
80	7411.0
81	7411.0
82	4933.0
83	2455.0
84	2455.0
85	2455.0
86	1564.5
87	674.0
88	674.0
89	674.0
90	435.0
91	196.0
92	196.0
93	196.0
94	113.0
95	30.0
96	30.0
97	30.0
98	20.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3322174235261046
2	0.010901389652219791
3	0.009866242860317615
4	0.011904188106875024
5	0.013004031573271086
6	0.07938281960399812
7	0.07834767281209595
8	0.10519679272705865
9	0.08226182161897605
10	0.09096352433840373
11	0.08601422873962145
12	0.09801546185823728
13	0.08213242826998829
14	0.0875669489274747
15	0.08384689014407626
16	0.08624066710035004
17	0.10021514879102941
18	0.10228544237483378
19	0.09510411150601242
20	0.09413366138860414
21	0.079447516278492
22	0.10251188073556237
23	0.09523350485500019
24	0.0905753442914404
25	0.08886088241735243
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3091349.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.95702410018769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.25414328276192	36.81856120598113
2	14.387587040423867	14.662972391248537
3	5.451763354429877	8.3341690992061
4	2.6135216128878316	5.327091352571464
5	1.4871977468446338	3.7891585713853404
6	0.9140958355756956	2.794776211398715
7	0.6154338782656898	2.1952475276799714
8	0.4367455603825999	1.7804203236852894
9	0.3123541274326522	1.4324973119440876
>10	1.401182077503071	12.297971888326162
>50	0.07611558453711686	2.648046430059768
>100	0.0434718819094038	4.107379885630842
>500	0.004216091250759982	1.4834990465525326
>1k	0.002108045624432801	2.1090399502517365
>5k	6.388017043735759E-5	0.21916880407842051
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6733	0.2178013546836672	No Hit
GTCCTACAGTGGACATTTCTAAATT	4623	0.14954636309261748	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3995	0.1292316073015373	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3943	0.12754949376469626	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3832	0.12395882833028558	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3806	0.12311777156186507	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3173	0.10264127408455015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.2348337246943E-5
7	0.0	0.0	0.0	0.0	3.2348337246943E-5
8	0.0	0.0	0.0	0.0	3.2348337246943E-5
9	0.0	0.0	0.0	0.0	3.2348337246943E-5
10	0.0	0.0	0.0	0.0	3.2348337246943E-5
11	0.0	0.0	0.0	0.0	6.4696674493886E-5
12	0.0	0.0	0.0	0.0	9.7045011740829E-5
13	0.0	3.2348337246943E-5	0.0	0.0	1.29393348987772E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAACG	210	0.0	13.570387	18
GGTATCA	2230	0.0	13.105907	1
TAGGACC	1005	0.0	11.908763	4
GCCGGTT	140	6.82121E-10	11.534455	11
TCGAACT	340	0.0	10.896047	19
TTAGGAC	1320	0.0	10.865887	3
CGTGCGC	210	0.0	10.855607	10
GTCCTAC	2545	0.0	10.847853	1
GTATCAA	4940	0.0	10.791819	1
TCCTACA	2860	0.0	10.561443	2
GTCGAGG	100	2.40294E-5	10.449029	19
GTCCTAT	470	0.0	10.3301325	1
GCGTGCG	175	2.3646862E-10	10.312659	9
TAGAAAT	710	0.0	10.167576	4
GTAGGAC	2505	0.0	10.162234	3
GTCGTAC	75	0.0026031898	10.154589	1
TACGCTC	75	0.0026519469	10.1319	4
TGTAGGA	2715	0.0	10.075923	2
TTTAGGA	1540	0.0	10.053772	2
GTCCTAA	1285	0.0	10.001479	1
>>END_MODULE