FastQCFastQC Report
Thu 26 May 2016
SRR1512897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512897_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091349
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT90080.29139382192046254No Hit
TATCAACGCAGAGTACTTTTTTTTT65840.21298145243387273No Hit
GGTATCAACGCAGAGTACTTTTTTT53960.17455162778450445No Hit
GTCCTACAGTGGACATTTCTAAATT43460.14058587367521427No Hit
CTGTAGGACGTGGAATATGGCAAGA38280.12382943498129781No Hit
GTCCTAAAGTGTGTATTTCTCATTT36270.11732741919466226No Hit
CTTTAGGACGTGAAATATGGCGAGG33180.10733178298535688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAA400.005309355414.23836619
GGTATCA23950.013.425251
TAGGACC9400.013.0495974
TACGGCG550.003086215812.08221116
CGATACT1001.9095223E-611.410814
GTATCAA50750.010.778121
GGCGAGG11450.010.61141719
GTCCTAC24650.010.4197681
GTAGGAC23950.010.4036883
CCGTACA2200.010.355844517
AGGACCT17600.010.2654085
TTAGGAC15100.010.2030413
TGTAGGA25800.010.1739122
AATACCG750.002628089910.1429435
ATAGGAC4050.010.0972953
TGGCGAG24500.010.07405418
GGACCTG16500.09.9700536
TTTAGGA17000.09.8460572
CCAACGA1356.521095E-79.84380919
TAGGACA8600.09.8407184
Produced by FastQC (version 0.11.5)