##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512897_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3091349
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.380355631150024	31.0	30.0	34.0	26.0	34.0
2	30.371646811796403	31.0	30.0	34.0	26.0	34.0
3	30.55606532940797	31.0	31.0	34.0	27.0	34.0
4	34.17207374515139	37.0	35.0	37.0	30.0	37.0
5	33.769935390666014	37.0	35.0	37.0	28.0	37.0
6	33.65967478922632	37.0	35.0	37.0	28.0	37.0
7	33.50384799645721	37.0	35.0	37.0	28.0	37.0
8	33.45125736369462	37.0	35.0	37.0	28.0	37.0
9	34.79604276320791	39.0	35.0	39.0	27.0	39.0
10	34.71856299628415	39.0	35.0	39.0	27.0	39.0
11	34.71734314048656	39.0	35.0	39.0	27.0	39.0
12	34.5770833380508	39.0	35.0	39.0	27.0	39.0
13	34.57560275465501	39.0	35.0	39.0	27.0	39.0
14	35.664733422204996	40.0	36.0	41.0	27.0	41.0
15	35.653900934511114	40.0	36.0	41.0	27.0	41.0
16	35.64609722163366	40.0	36.0	41.0	27.0	41.0
17	35.63129591644295	40.0	36.0	41.0	27.0	41.0
18	35.551059424219005	40.0	36.0	41.0	27.0	41.0
19	35.568163607538324	40.0	36.0	41.0	27.0	41.0
20	35.535487905118444	40.0	36.0	41.0	26.0	41.0
21	35.504034646363124	40.0	36.0	41.0	26.0	41.0
22	35.46112393003831	40.0	36.0	41.0	26.0	41.0
23	35.30493451240866	40.0	36.0	41.0	25.0	41.0
24	35.26224247084364	40.0	36.0	41.0	25.0	41.0
25	35.20551642664739	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5283310881012824
1101	2	0.43818527756446457
1101	3	0.3499822738292373
1101	4	0.40000478708147824
1101	5	0.4916940250537465
1101	6	0.5619727117340361
1101	7	0.5992358780328217
1101	8	0.3879178911235215
1101	9	0.6681021245453422
1101	10	0.3842709011688754
1101	11	0.7192232299813881
1101	12	0.8395760407448307
1101	13	1.0303627840103218
1101	14	1.0304228392118233
1101	15	0.7418366123826843
1101	16	0.9847941978286201
1101	17	0.9529660210008615
1101	18	0.8437821932218483
1101	19	0.7066678565247173
1101	20	0.7450684375750569
1101	21	0.8532415111376892
1101	22	0.7571635935359495
1101	23	0.8784778596318645
1101	24	0.9232930190941957
1101	25	0.9759514113333125
1102	1	0.6598862255111015
1102	2	0.7123435834603882
1102	3	0.373549781387144
1102	4	0.4851135725217688
1102	5	0.6574615734556275
1102	6	0.5585950932318013
1102	7	0.5334046664279057
1102	8	0.6063565018073689
1102	9	0.8019582994628749
1102	10	0.7754075958091562
1102	11	0.7914682073669184
1102	12	0.7386659139187586
1102	13	0.8058329459814573
1102	14	0.8039843763580947
1102	15	0.9802149450641906
1102	16	0.9514231835551143
1102	17	0.9623193178167639
1102	18	0.3245228257367927
1102	19	0.6521119148573149
1102	20	0.9076344832674508
1102	21	0.7874211052968079
1102	22	0.614812680616879
1102	23	0.7785561878003762
1102	24	0.8046518593578469
1102	25	0.8193068789273212
1103	1	0.494332161578928
1103	2	0.7185657436416406
1103	3	0.4795938086584606
1103	4	0.3244338791144372
1103	5	0.39736105659763865
1103	6	0.472288073579179
1103	7	0.551874861836005
1103	8	0.6162042152908214
1103	9	0.595495425743664
1103	10	0.5817460595193822
1103	11	0.7589922332964747
1103	12	0.8563520869194292
1103	13	0.823337788904432
1103	14	0.8238655185890806
1103	15	0.8241240609407328
1103	16	0.8028109640148671
1103	17	0.8576195860294433
1103	18	0.7696692105961205
1103	19	0.7918132958940234
1103	20	0.6215914832828275
1103	21	0.8727811168695041
1103	22	0.777938469426303
1103	23	0.7658658776995608
1103	24	0.6656434600661854
1103	25	0.6344178119132877
1104	1	0.5843906889727002
1104	2	0.7693919599636985
1104	3	0.61817540528385
1104	4	0.5031200421545918
1104	5	0.6599337929540638
1104	6	0.4821570746665529
1104	7	0.5044942249819613
1104	8	0.6441744369547422
1104	9	0.7010704017715881
1104	10	0.7480598657238886
1104	11	0.7889589030614559
1104	12	0.791910955697368
1104	13	0.5884093958567789
1104	14	0.8012104587562945
1104	15	0.8874671841513688
1104	16	0.7936179394604892
1104	17	0.7775304844199837
1104	18	0.4579324980948982
1104	19	0.832778968844579
1104	20	0.8729496301306483
1104	21	0.7707779025957535
1104	22	0.8270538777784964
1104	23	0.7755617519061602
1104	24	1.0353799842764388
1104	25	0.8616466507082023
1105	1	0.5948766429373613
1105	2	0.7175458589435735
1105	3	0.6874351816417636
1105	4	0.5258566963033289
1105	5	0.5101936896508619
1105	6	0.6655653845035516
1105	7	0.7279125249632656
1105	8	0.6608531795276775
1105	9	0.8800860061618749
1105	10	0.6354457370648134
1105	11	0.8954457488112055
1105	12	0.9312664518902238
1105	13	0.9062673754677348
1105	14	1.124654826560331
1105	15	1.1269239599701137
1105	16	1.0442779965643183
1105	17	0.8562577040673531
1105	18	0.8678074969184024
1105	19	1.0693447542137875
1105	20	1.1220596938228553
1105	21	0.9448943613921159
1105	22	0.8804711491648618
1105	23	1.0765527493474423
1105	24	1.0594485596253946
1105	25	0.8367587289002429
1106	1	0.05560541133036523
1106	2	0.30852106289376735
1106	3	-1.5544766256602358
1106	4	-0.34262422404534476
1106	5	-0.074054480420088
1106	6	0.04576304751036275
1106	7	0.07264261894733437
1106	8	0.19022239071580316
1106	9	0.4045270599556545
1106	10	0.1490709134127286
1106	11	0.26617783118187077
1106	12	0.3718854671362237
1106	13	0.3950570580074171
1106	14	0.3797206971908196
1106	15	0.405112918035087
1106	16	0.3489327720998361
1106	17	0.3751196466546105
1106	18	0.4532349404018774
1106	19	0.4482062197245682
1106	20	0.4450191695839649
1106	21	0.486515748389742
1106	22	0.47809488235643727
1106	23	0.5483070106626755
1106	24	0.5373706104408527
1106	25	0.45859694715591104
1107	1	0.5921337743955704
1107	2	0.6792238051070782
1107	3	0.576555454227158
1107	4	0.40560464575240474
1107	5	0.5821965557284727
1107	6	0.5408330708719333
1107	7	0.4864161508301308
1107	8	0.3691876129419782
1107	9	0.5380598330355326
1107	10	0.48294041267293863
1107	11	0.6085061300552681
1107	12	0.7579549294955044
1107	13	0.6071513271932503
1107	14	0.7818021259348953
1107	15	0.6754459645229787
1107	16	0.7330793923470509
1107	17	0.768714360877297
1107	18	0.7988272866415898
1107	19	0.6772197938167039
1107	20	0.8162269178638795
1107	21	0.7026811011628311
1107	22	0.7697098638009265
1107	23	0.8664174833918707
1107	24	0.8802897402029473
1107	25	0.803321767939913
1108	1	0.4380236821921848
1108	2	0.3337436104992868
1108	3	0.42613254182336036
1108	4	0.34686957251169304
1108	5	0.5293150058420082
1108	6	0.5898565081386309
1108	7	0.36924212067381035
1108	8	0.4034114453489792
1108	9	0.5077233784760722
1108	10	0.525177303344023
1108	11	0.4769633508620075
1108	12	0.5168347760262648
1108	13	0.6190376682155758
1108	14	0.7193967153318042
1108	15	0.6686515342333479
1108	16	0.5941911604326577
1108	17	0.6414131285125819
1108	18	0.7503331319229005
1108	19	0.701114792568454
1108	20	0.7046621359973315
1108	21	0.711585733549768
1108	22	0.6956031400849838
1108	23	0.7471678916119302
1108	24	0.7726033774407384
1108	25	0.7436549854068346
1109	1	0.173343796222035
1109	2	0.1360149801687065
1109	3	0.2422676681643523
1109	4	0.23689147517838194
1109	5	-0.13825909170498818
1109	6	0.13783233264533834
1109	7	0.3339159360600661
1109	8	0.32021312180142303
1109	9	0.2950322157892771
1109	10	0.02519187274536705
1109	11	0.2417600348662603
1109	12	0.44544009580034327
1109	13	0.37658874610845317
1109	14	0.4828199904360986
1109	15	0.48524268283638605
1109	16	0.41284193673892844
1109	17	0.5489175843211598
1109	18	0.6613973781263596
1109	19	0.6089465742605427
1109	20	0.6146113373513131
1109	21	0.6137779428052212
1109	22	0.531385346960775
1109	23	0.5819705490707392
1109	24	0.45536389047792625
1109	25	0.45820301829483157
1110	1	0.2872777151584849
1110	2	0.10989113747420731
1110	3	0.18233139584559055
1110	4	0.2136963697773595
1110	5	0.26489439087261957
1110	6	0.29821712524730515
1110	7	0.16777157945448806
1110	8	0.21907874630174717
1110	9	0.2262391253094762
1110	10	0.36826766997086224
1110	11	0.31401600055570356
1110	12	0.36434387094696064
1110	13	0.3229759257720488
1110	14	0.3246568298664272
1110	15	0.3590373041213013
1110	16	0.30809236731294476
1110	17	0.4104178519113475
1110	18	0.4321442378107889
1110	19	0.42262790634485015
1110	20	0.4055974613126878
1110	21	0.3993433407399749
1110	22	0.36796219891018467
1110	23	0.43786168693605987
1110	24	0.4036818572223311
1110	25	0.33386988880155144
1111	1	0.01643471150674003
1111	2	-0.07321307387026366
1111	3	-0.11351901129012276
1111	4	0.02905471756891842
1111	5	0.18793629315430138
1111	6	0.22951459100359983
1111	7	0.07186611339918159
1111	8	0.11435087505176966
1111	9	0.1678307082430308
1111	10	-0.0010380365738811292
1111	11	-0.003622891838375608
1111	12	0.15983093234045498
1111	13	0.13021168528644012
1111	14	0.06399669146999543
1111	15	0.03560712224956575
1111	16	0.06300450305275263
1111	17	0.27716013208218016
1111	18	0.07512651768772116
1111	19	-0.0674254290883951
1111	20	0.11056503133750795
1111	21	0.17389439570848708
1111	22	-0.07989674866739449
1111	23	0.14193796374553358
1111	24	0.17935884872756702
1111	25	0.10934186689426184
1112	1	0.13535188122303765
1112	2	-0.27387019608071483
1112	3	0.04476677904776949
1112	4	0.1864383054716896
1112	5	0.043754992096253886
1112	6	0.08700977209662142
1112	7	0.08926206877999476
1112	8	0.07261604754231143
1112	9	0.09555982316317824
1112	10	-0.11785397064070224
1112	11	-0.05858391094311344
1112	12	0.31043485840499585
1112	13	0.15108299567702943
1112	14	-0.0935436905989846
1112	15	-0.22520598168861738
1112	16	-0.27618278872959934
1112	17	-0.16732177121405556
1112	18	0.027217036579095577
1112	19	-0.06985963530046746
1112	20	-0.05575033240719307
1112	21	-0.08707118177375861
1112	22	0.009442168686447872
1112	23	0.26318576588138853
1112	24	0.22861313357334723
1112	25	0.22229350919908342
1113	1	-0.5611327079977322
1113	2	-0.5249980645664252
1113	3	-0.5620653198789789
1113	4	-2.146468256780217
1113	5	-0.7044187642415878
1113	6	-0.30355468926853035
1113	7	-0.310057448504665
1113	8	-0.3520630017963313
1113	9	-0.30459900645703186
1113	10	-0.38270001133355436
1113	11	-0.5857209340335459
1113	12	-0.19597623561381994
1113	13	-0.3583434606158704
1113	14	-0.5162199226792978
1113	15	-0.447946210870505
1113	16	-0.7082680300971731
1113	17	-0.7553893062219075
1113	18	-0.6076422539390336
1113	19	-0.3982202008111244
1113	20	-0.1917507313373079
1113	21	-0.30831901141574747
1113	22	-0.24743281770241055
1113	23	-0.19117843553575398
1113	24	-0.12383098156401928
1113	25	-0.3287289595744909
1114	1	-0.8446413116460008
1114	2	-2.074620018237237
1114	3	-1.363724544347427
1114	4	-0.8322930544111671
1114	5	-0.8464829585979743
1114	6	-0.4023653530927973
1114	7	-0.453298044545825
1114	8	-0.5535738335035703
1114	9	-0.8032778658720332
1114	10	-0.8136935288710205
1114	11	-0.8070534750187193
1114	12	-0.7871297161774038
1114	13	-0.8540897205839215
1114	14	-0.8979382052195461
1114	15	-1.0564886636038722
1114	16	-1.387123242714722
1114	17	-1.3892462475669092
1114	18	-1.0856176557575807
1114	19	-1.533096861327273
1114	20	-0.9442881298873687
1114	21	-1.1827093563048408
1114	22	-0.8149738188001621
1114	23	-0.6413499900598509
1114	24	-0.7248312193746358
1114	25	-1.3506351390408184
1115	1	-0.8217671519485066
1115	2	-1.0193246457424507
1115	3	-0.5577187757144735
1115	4	-0.3503114462658985
1115	5	-0.6096634390795899
1115	6	-0.564687019051533
1115	7	-0.45906848742247064
1115	8	-0.6737987287860747
1115	9	-1.4770886360292934
1115	10	-1.2539158072647325
1115	11	-1.5299970387745248
1115	12	-2.192220394817724
1115	13	-2.240430797076975
1115	14	-1.622542707592622
1115	15	-1.840693382355866
1115	16	-1.6138848242796797
1115	17	-1.435263407259022
1115	18	-1.7921659785428687
1115	19	-2.2176161276413566
1115	20	-2.4236270246685763
1115	21	-1.6051347160409648
1115	22	-1.5661331649911503
1115	23	-1.6041650949468576
1115	24	-2.0842254926083754
1115	25	-1.9944166687323985
1116	1	-1.1165434520967352
1116	2	-1.300603914260634
1116	3	-1.3533060991751675
1116	4	-0.9337378808731387
1116	5	-1.3242428020962933
1116	6	-2.070902629469156
1116	7	-1.6175018299359394
1116	8	-1.8182191246654362
1116	9	-2.934782967130964
1116	10	-1.5232335195924236
1116	11	-2.462449027571907
1116	12	-4.651379913584101
1116	13	-3.5586449457278206
1116	14	-2.8836497952366855
1116	15	-4.1240257745658475
1116	16	-3.432887144734096
1116	17	-3.164485068446041
1116	18	-3.289351867676835
1116	19	-3.5439212156278757
1116	20	-5.353340891849037
1116	21	-4.183196428251161
1116	22	-3.2888131370438956
1116	23	-4.208420200981962
1116	24	-4.075640440706259
1116	25	-3.2390957108518776
1201	1	0.5586784653694963
1201	2	0.7794957301329255
1201	3	0.8260422972373931
1201	4	0.705213597214545
1201	5	0.18327852577509418
1201	6	0.668973499574399
1201	7	0.9179410302826696
1201	8	0.8035512258995112
1201	9	0.8055636789828284
1201	10	0.9376006318694508
1201	11	0.6980001951639565
1201	12	1.1566060707061183
1201	13	1.1162004058575121
1201	14	1.1258594446606125
1201	15	0.9696899897396847
1201	16	1.0474729180852407
1201	17	0.8355809114524959
1201	18	1.1714279110207855
1201	19	1.2898181929810235
1201	20	1.289280961892132
1201	21	1.0134249062134515
1201	22	1.0043231838960338
1201	23	1.0710574246969031
1201	24	0.8908392971856216
1201	25	1.0781638980238526
1202	1	0.8578624491491773
1202	2	0.8901912421020626
1202	3	0.8429627325812952
1202	4	0.7265730132154857
1202	5	0.7614836186477589
1202	6	0.8317736773544553
1202	7	0.902846916100728
1202	8	0.7492226037138323
1202	9	1.0540054328077133
1202	10	1.1418413200732687
1202	11	1.0834002366555495
1202	12	1.138834932101318
1202	13	1.081583744784993
1202	14	1.2796936418634388
1202	15	1.1564396210327814
1202	16	1.233538683241946
1202	17	1.036871720006161
1202	18	1.041156753339905
1202	19	1.2519084175284405
1202	20	1.0575609380169624
1202	21	1.2604766199767852
1202	22	1.0771753500451595
1202	23	1.2772262778709873
1202	24	1.1275546498906124
1202	25	1.2687874092482616
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2211	8	-0.29728875874377536
2211	9	-0.15138424499338754
2211	10	-0.11004007186596709
2211	11	0.01280735362539076
2211	12	-0.09003849555934096
2211	13	0.02250325215501192
2211	14	-0.1689749056687404
2211	15	-0.1485854649917897
2211	16	-0.19222650226237192
2211	17	-0.25273061151309406
2211	18	-0.1989863578743325
2211	19	-0.2248581417462887
2211	20	-0.1567580950138705
2211	21	-0.13985466550209935
2211	22	-0.10698547306171236
2211	23	-0.16360664183835638
2211	24	-0.02363864570197194
2211	25	-0.173766807806075
2212	1	-0.07533771341858042
2212	2	0.06459943145931035
2212	3	0.08351605672276818
2212	4	-0.018242348130563357
2212	5	-0.28190713691449787
2212	6	-0.020365399526063754
2212	7	-0.16614882378792117
2212	8	0.005148067323410999
2212	9	-0.06460255545610494
2212	10	-0.2500726521372698
2212	11	0.04027754218542867
2212	12	0.2340345309358156
2212	13	0.18454755095475406
2212	14	0.27876607431617373
2212	15	-0.06037804891044374
2212	16	-0.047716000199500286
2212	17	-0.1964970994686297
2212	18	-0.14293357415986208
2212	19	0.09872933372005122
2212	20	0.02536240385325783
2212	21	0.1634843788177065
2212	22	0.11063356360970289
2212	23	-0.29567199268488054
2212	24	-0.25491893252429776
2212	25	-0.16848294118254614
2213	1	-0.3863879138512374
2213	2	-0.010240431281516749
2213	3	-0.16477639246589249
2213	4	-0.31528995553455275
2213	5	-0.17959787123982807
2213	6	-0.011414439485228911
2213	7	-0.4646838621324676
2213	8	-0.3696926547123027
2213	9	-0.08612763712527993
2213	10	-0.1069210154314959
2213	11	-0.0640045284225792
2213	12	-0.17886925364558692
2213	13	-0.24776485497478973
2213	14	-0.20625875547749928
2213	15	-0.5027218718521382
2213	16	-1.0286698991990022
2213	17	-0.6240475653893824
2213	18	-0.3945204408329417
2213	19	-0.31045319353086853
2213	20	-0.21090795686257024
2213	21	-0.1183221448980234
2213	22	-0.3405366641536389
2213	23	-0.5054654360607103
2213	24	-0.32725756651598203
2213	25	-0.30366517082816813
2214	1	-1.2079011348404158
2214	2	-0.4767571827859385
2214	3	-0.61782185707116
2214	4	-0.6566093670450854
2214	5	-1.0162988945623113
2214	6	-1.584290070498966
2214	7	-1.0261158812710178
2214	8	-0.8970056691399932
2214	9	-0.6923687449693432
2214	10	-0.9464704268366404
2214	11	-1.0342833864326337
2214	12	-0.721388776867947
2214	13	-1.110559547609185
2214	14	-0.760563876305433
2214	15	-0.8821289560681009
2214	16	-0.9556245926765072
2214	17	-0.9888686156913167
2214	18	-1.0300368972495235
2214	19	-0.9855477797228644
2214	20	-1.2005018949151989
2214	21	-1.3501062670643904
2214	22	-1.7477025562147261
2214	23	-1.5656103979627645
2214	24	-1.2593391477652602
2214	25	-1.6794334531007635
2215	1	-1.412782777858986
2215	2	-0.7662091365543091
2215	3	-0.9044667753736562
2215	4	-0.8298117890260812
2215	5	-1.2723866443279803
2215	6	-1.1221143917474805
2215	7	-1.5656717379164142
2215	8	-1.2709478670599665
2215	9	-1.3414743468533104
2215	10	-1.31463667323208
2215	11	-1.0597789749288467
2215	12	-1.381831217631209
2215	13	-1.4306386553796315
2215	14	-1.7116535347656097
2215	15	-1.5268873942004078
2215	16	-1.3646021776134134
2215	17	-1.4551703453449036
2215	18	-1.1678459847860125
2215	19	-1.4227870368195354
2215	20	-1.58170779555914
2215	21	-1.099836630361537
2215	22	-1.44060839574459
2215	23	-1.9791455959024518
2215	24	-1.7932349187075758
2215	25	-2.0027914381645573
2216	1	-1.2710955792848608
2216	2	-0.6496845332132892
2216	3	-0.6848891647634652
2216	4	-0.9707813755920967
2216	5	-1.68499013387855
2216	6	-1.8672654978493917
2216	7	-1.7625368214419588
2216	8	-1.789404173413498
2216	9	-3.7058943859949878
2216	10	-1.8218336746998887
2216	11	-1.9885942838685295
2216	12	-2.2955554435346173
2216	13	-2.1794965134211495
2216	14	-2.2630371669037217
2216	15	-2.2903554517945963
2216	16	-1.6716983106252243
2216	17	-1.9776025900502887
2216	18	-2.3511426664802926
2216	19	-2.4970114549365405
2216	20	-1.8024623650006717
2216	21	-2.5805549850131086
2216	22	-1.909561530573697
2216	23	-3.0300975774499435
2216	24	-2.3978594469105587
2216	25	-2.4194014018187175
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77688.0
3	0.0
4	1319.0
5	10939.0
6	13277.0
7	10237.0
8	8402.0
9	7311.0
10	6220.0
11	5814.0
12	5270.0
13	4815.0
14	4879.0
15	4936.0
16	5021.0
17	5499.0
18	6114.0
19	7023.0
20	9222.0
21	12211.0
22	15366.0
23	19050.0
24	22642.0
25	25696.0
26	29010.0
27	31874.0
28	36332.0
29	43971.0
30	55077.0
31	69787.0
32	88762.0
33	114211.0
34	148467.0
35	195490.0
36	267800.0
37	414603.0
38	1307014.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.853139336486755	18.979962508620105	13.636275338248005	26.53062281664514
2	13.771537495631012	19.947021967921916	40.278256029204265	26.00318450724281
3	17.702558374971	24.83690857011775	29.054200742796816	28.406332312114436
4	11.790906102885419	16.57301153494776	36.58926450150236	35.04681786066446
5	13.328225518646489	38.4922261670456	34.34224536988354	13.837302944424366
6	32.75155615845806	36.08555950333695	17.707790162741595	13.455094175463401
7	29.213998243307426	31.30412570226424	21.01497721253099	18.466898841897343
8	26.42965526438103	36.18526416093445	19.70512915124374	17.67995142344078
9	27.391146222959662	14.632973615206007	19.37317023925699	38.60270992257734
10	15.966693885582812	28.83334417558887	32.750246539944975	22.449715398883345
11	35.15239215503587	22.25217915670259	23.532369017671357	19.063059670590185
12	23.952696287665265	24.555346980260683	30.520359597754116	20.97159713431994
13	29.426898919986478	21.35366439513905	25.481622624479765	23.737814060394705
14	21.831436518098084	21.389813160841868	27.055156337644544	29.72359398341551
15	23.906098195009854	29.888794023548243	22.90876114672246	23.29634663471944
16	23.106551879981897	27.454694446933697	26.581339065118936	22.85741460796547
17	22.15519310661188	28.05145843321383	26.967799497540433	22.825548962633857
18	22.115660835876056	26.864500759581468	28.86894825787985	22.15089014666263
19	23.988565335900535	26.150987512705004	27.279023531185242	22.581423620209222
20	24.450450537879632	26.119648497964455	27.507703057446616	21.922197906709304
21	24.161876674232964	25.752157397064902	26.640574430320047	23.445391498382094
22	24.121116526603213	26.81749029626987	26.782553956427268	22.278839220699652
23	23.253861016662952	26.501957559628497	27.212165303885133	23.032016119823417
24	23.70689947980639	26.053253773676154	27.549364371347266	22.69048237517019
25	23.26629030442383	26.35251676614862	27.596019600510846	22.785173328916706
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	77.0
1	77.0
2	356.0
3	635.0
4	635.0
5	635.0
6	1503.0
7	2371.0
8	2371.0
9	2371.0
10	3084.0
11	3797.0
12	3797.0
13	3797.0
14	6205.5
15	8614.0
16	8614.0
17	8614.0
18	17548.5
19	26483.0
20	26483.0
21	26483.0
22	44159.5
23	61836.0
24	61836.0
25	61836.0
26	93933.5
27	126031.0
28	126031.0
29	126031.0
30	164277.0
31	202523.0
32	202523.0
33	202523.0
34	234181.0
35	265839.0
36	265839.0
37	265839.0
38	291860.0
39	317881.0
40	317881.0
41	317881.0
42	341503.0
43	365125.0
44	365125.0
45	365125.0
46	389010.0
47	412895.0
48	412895.0
49	412895.0
50	408352.0
51	403809.0
52	403809.0
53	403809.0
54	365444.5
55	327080.0
56	327080.0
57	327080.0
58	284931.0
59	242782.0
60	242782.0
61	242782.0
62	203090.0
63	163398.0
64	163398.0
65	163398.0
66	125951.0
67	88504.0
68	88504.0
69	88504.0
70	66028.5
71	43553.0
72	43553.0
73	43553.0
74	30882.0
75	18211.0
76	18211.0
77	18211.0
78	12566.5
79	6922.0
80	6922.0
81	6922.0
82	4563.0
83	2204.0
84	2204.0
85	2204.0
86	1417.0
87	630.0
88	630.0
89	630.0
90	384.5
91	139.0
92	139.0
93	139.0
94	74.0
95	9.0
96	9.0
97	9.0
98	5.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08536726199468259
2	0.04493184043600383
3	0.019344305673671916
4	0.007148982531574403
5	0.02134990258298238
6	0.05984442390684455
7	0.08378219346958238
8	0.05800056868376881
9	0.025264051389862482
10	0.051304462873651605
11	0.007925342625501035
12	1.94090023481658E-4
13	0.005952094053437512
14	8.73405105667461E-4
15	0.013521604969222173
16	0.009122231103637926
17	0.005013992273276166
18	0.0055962623437211395
19	0.0015203718506063212
20	0.001423326838865492
21	2.58786697975544E-4
22	3.88180046963316E-4
23	0.0
24	0.0
25	3.2348337246943E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3091349.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.69170266030746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.11613349683053	34.14073923918097
2	15.332291652296487	14.931107724694694
3	5.8522195751629615	8.548636063699973
4	2.882415082887167	5.613987926381097
5	1.6494893020463581	4.015822131829968
6	1.014691367792745	2.964423022352701
7	0.6453958365257645	2.199779551921905
8	0.4706030858676281	1.8331572422471754
9	0.3360015596970718	1.472443923435243
>10	1.5615882861104327	13.032043275130187
>50	0.08487732989462961	2.8394240288183483
>100	0.04817693259661426	4.456817884307025
>500	0.0038560494885057153	1.3868165917298292
>1k	0.0020609919675819268	1.8853778036291513
>5k	1.9945083557244454E-4	0.6794235906417924
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9008	0.29139382192046254	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6584	0.21298145243387273	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5396	0.17455162778450445	No Hit
GTCCTACAGTGGACATTTCTAAATT	4346	0.14058587367521427	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3828	0.12382943498129781	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3627	0.11732741919466226	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3318	0.10733178298535688	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.2348337246943E-5	0.0
2	0.0	0.0	0.0	3.2348337246943E-5	0.0
3	0.0	0.0	0.0	3.2348337246943E-5	0.0
4	0.0	0.0	0.0	3.2348337246943E-5	3.2348337246943E-5
5	0.0	0.0	0.0	3.2348337246943E-5	3.2348337246943E-5
6	0.0	0.0	0.0	3.2348337246943E-5	3.2348337246943E-5
7	0.0	0.0	0.0	3.2348337246943E-5	3.2348337246943E-5
8	0.0	0.0	0.0	3.2348337246943E-5	3.2348337246943E-5
9	0.0	0.0	0.0	6.4696674493886E-5	3.2348337246943E-5
10	3.2348337246943E-5	0.0	0.0	6.4696674493886E-5	3.2348337246943E-5
11	3.2348337246943E-5	6.4696674493886E-5	0.0	6.4696674493886E-5	3.2348337246943E-5
12	6.4696674493886E-5	6.4696674493886E-5	0.0	6.4696674493886E-5	1.94090023481658E-4
13	6.4696674493886E-5	6.4696674493886E-5	0.0	6.4696674493886E-5	2.2643836072860102E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGTAA	40	0.0053093554	14.238366	19
GGTATCA	2395	0.0	13.42525	1
TAGGACC	940	0.0	13.049597	4
TACGGCG	55	0.0030862158	12.082211	16
CGATACT	100	1.9095223E-6	11.41081	4
GTATCAA	5075	0.0	10.77812	1
GGCGAGG	1145	0.0	10.611417	19
GTCCTAC	2465	0.0	10.419768	1
GTAGGAC	2395	0.0	10.403688	3
CCGTACA	220	0.0	10.3558445	17
AGGACCT	1760	0.0	10.265408	5
TTAGGAC	1510	0.0	10.203041	3
TGTAGGA	2580	0.0	10.173912	2
AATACCG	75	0.0026280899	10.142943	5
ATAGGAC	405	0.0	10.097295	3
TGGCGAG	2450	0.0	10.074054	18
GGACCTG	1650	0.0	9.970053	6
TTTAGGA	1700	0.0	9.846057	2
CCAACGA	135	6.521095E-7	9.843809	19
TAGGACA	860	0.0	9.840718	4
>>END_MODULE