FastQCFastQC Report
Thu 26 May 2016
SRR1512896_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512896_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2364492
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT124180.525186805453349No Hit
TATCAACGCAGAGTACTTTTTTTTT73430.31055296444225655No Hit
GGTATCAACGCAGAGTACTTTTTTT70980.30019133073827275No Hit
GTCCTACAGTGGACATTTCTAAATT53450.22605278427670722No Hit
GTCCTAAAGTGTGTATTTCTCATTT48060.2032571901279429No Hit
CTGTAGGACGTGGAATATGGCAAGA46390.19619436225624784No Hit
CTTTAGGACGTGAAATATGGCGAGG42760.18084222742136577No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32650.13808462874900823No Hit
GTCCTACAGTGTGCATTTCTCATTT27460.11613488224954875No Hit
GTACTGGTTCACTATCGGTCAGTCA26940.11393567836135628No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25620.10835308387594461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21000.014.5977681
ATACGTA400.005284161314.2483816
TAGGACC9350.013.7150194
ATATCGC604.0966208E-412.66549515
GCACCGT1352.7284841E-1112.6652276
TCCAACG1207.421477E-1012.66495918
ACACCGT701.09252185E-412.2128986
AGGACCT17600.011.8196795
ATCGCAA752.0768763E-411.39870517
CGCGCCA600.00588401911.08160410
GGACCTG17500.010.9644686
CGTGCGC1303.2761818E-810.95982810
GACGGTT700.001493578210.855679518
CGCCCTA4050.010.78889716
TGTAGGA29250.010.7492062
AGCACCG1158.8349225E-710.737915
GTATCAA46550.010.716711
GTCCTAC26050.010.7080351
ACGGTTG803.7769412E-410.68605919
CTGTAGG28250.010.5818391
Produced by FastQC (version 0.11.5)