##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512896_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2364492
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.849411205451318	31.0	30.0	34.0	22.0	34.0
2	29.039655452418533	31.0	30.0	34.0	22.0	34.0
3	29.128897454506085	31.0	30.0	34.0	22.0	34.0
4	32.560407478646574	35.0	33.0	37.0	24.0	37.0
5	32.75493594395752	35.0	33.0	37.0	26.0	37.0
6	32.632932570717095	37.0	33.0	37.0	25.0	37.0
7	32.55158190427373	37.0	33.0	37.0	23.0	37.0
8	32.451040223439115	37.0	33.0	37.0	23.0	37.0
9	33.73061274895411	39.0	34.0	39.0	21.0	39.0
10	33.604307817493144	38.0	34.0	39.0	19.0	39.0
11	33.64542912388792	38.0	34.0	39.0	19.0	39.0
12	33.54545923606424	38.0	34.0	39.0	18.0	39.0
13	33.5031000316347	38.0	34.0	39.0	17.0	39.0
14	34.35957533372919	39.0	34.0	41.0	17.0	41.0
15	34.53329636979106	39.0	34.0	41.0	17.0	41.0
16	34.48230275255742	39.0	34.0	41.0	17.0	41.0
17	34.263200721338876	39.0	34.0	41.0	17.0	41.0
18	34.26482136543494	39.0	34.0	41.0	17.0	41.0
19	34.386579019933244	39.0	34.0	41.0	18.0	41.0
20	34.354345034789716	39.0	34.0	41.0	18.0	41.0
21	34.26354667302744	39.0	34.0	41.0	16.0	41.0
22	34.27485523317482	39.0	34.0	41.0	15.0	41.0
23	33.950301798441274	39.0	34.0	41.0	9.0	41.0
24	34.02165623736515	39.0	34.0	41.0	2.0	41.0
25	33.743881983952576	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6001392211486589
1101	2	0.23211374079660985
1101	3	0.5611615951404225
1101	4	0.6712060808087301
1101	5	0.5906370979002915
1101	6	0.5553648638133595
1101	7	0.3632877933844938
1101	8	0.6866579204828653
1101	9	0.8293942842338566
1101	10	0.7052387983150368
1101	11	0.8405226087359665
1101	12	0.6724078727565228
1101	13	0.9583315664800693
1101	14	1.0871465007942263
1101	15	0.989294658626946
1101	16	0.88816205739883
1101	17	0.20527130414438233
1101	18	0.6854453833119294
1101	19	0.17669102678779325
1101	20	-0.42000222453202696
1101	21	0.6748274272488715
1101	22	0.7685464571399478
1101	23	0.17967356649664623
1101	24	0.8421993917557131
1101	25	0.515220031169811
1102	1	0.9966855726117956
1102	2	1.0324283393176046
1102	3	0.8941476741441541
1102	4	1.080769978937596
1102	5	0.9638320730710532
1102	6	0.9275675126826144
1102	7	0.894798487953544
1102	8	0.9492837224037274
1102	9	1.2764314256343425
1102	10	1.2564043638894802
1102	11	1.0844705585426553
1102	12	1.2502422272086662
1102	13	1.1205474734410075
1102	14	1.3315019713502707
1102	15	1.2219137190393567
1102	16	1.2766282830038165
1102	17	1.4603092384062393
1102	18	1.4121504726197358
1102	19	1.2373064190448702
1102	20	1.470516756031941
1102	21	1.58399231742289
1102	22	1.43032282274838
1102	23	1.414120690952764
1102	24	0.890467873735453
1102	25	1.2302354916542058
1103	1	0.587429707687054
1103	2	0.6497332796128141
1103	3	0.774060716382774
1103	4	0.8652768381333047
1103	5	0.702053389658083
1103	6	0.3052626300175092
1103	7	0.2821870646962239
1103	8	0.4574847895919376
1103	9	0.4886559537347779
1103	10	0.7521067505037635
1103	11	0.46725608234571325
1103	12	0.6368813092763617
1103	13	0.7801493464800657
1103	14	0.8457443417310628
1103	15	0.780494568616291
1103	16	0.7981759515931088
1103	17	0.4836517814346948
1103	18	0.8940374824301998
1103	19	0.7978358486160246
1103	20	0.9145352516556926
1103	21	0.6168775716383834
1103	22	0.5822462426522463
1103	23	-0.0415067825919877
1103	24	0.20872572780874776
1103	25	0.5766643815649317
1104	1	0.9512184772471173
1104	2	0.860620711441257
1104	3	0.8566928292397407
1104	4	0.9679926491389637
1104	5	0.7978313664523924
1104	6	0.6955667603981226
1104	7	0.8974899179189819
1104	8	0.6796810632102321
1104	9	1.0708267258992024
1104	10	1.0614766060337573
1104	11	0.7868609244942135
1104	12	1.0308120466603867
1104	13	1.0797429496394955
1104	14	1.2408531920700554
1104	15	1.0283611937188581
1104	16	1.0080817963887156
1104	17	1.2673267849012717
1104	18	1.008356663464454
1104	19	0.5032169056022866
1104	20	0.7101931234832577
1104	21	0.8484221194254573
1104	22	0.7025737997568413
1104	23	1.0896437750307726
1104	24	0.8749459973985267
1104	25	0.5206117480654413
1105	1	0.6319497085779417
1105	2	0.6358288317795306
1105	3	0.6900435966355509
1105	4	0.822782200038489
1105	5	0.5091729643105154
1105	6	0.7851680044304814
1105	7	0.765317030847001
1105	8	0.6859332199449852
1105	9	0.5044958645990505
1105	10	0.7041937894060979
1105	11	0.5943346733250863
1105	12	0.7583841236036264
1105	13	0.8272050517374581
1105	14	0.8781284643263163
1105	15	0.8291750332867167
1105	16	0.8526369195340706
1105	17	1.0571466462332708
1105	18	1.0405413550524827
1105	19	0.6841351391674948
1105	20	0.9653649770757013
1105	21	0.8315770165903018
1105	22	0.8265348051159123
1105	23	1.105858475802357
1105	24	1.1368716996576609
1105	25	1.3805805303799445
1106	1	0.6559685784739564
1106	2	0.6652753122498076
1106	3	0.5645951657084254
1106	4	0.723332120510932
1106	5	0.5040646249465013
1106	6	0.6578020112648204
1106	7	0.5820843808185572
1106	8	0.6453954511283015
1106	9	0.7271788263523007
1106	10	0.49372827263178465
1106	11	0.2572481295423614
1106	12	0.5810512185452126
1106	13	0.5804780653867851
1106	14	0.7936841316805499
1106	15	0.5363781787853839
1106	16	0.5257922091807785
1106	17	0.7534500661116752
1106	18	0.6914327989710429
1106	19	0.8073699563702164
1106	20	0.8422907629666199
1106	21	0.7850485588830907
1106	22	0.7156993207331155
1106	23	1.0659437695899214
1106	24	0.7509444006146992
1106	25	0.9308009242638633
1107	1	0.9695520867307259
1107	2	0.875127264795136
1107	3	0.835156043660426
1107	4	0.9289290090792903
1107	5	0.7829886353457596
1107	6	0.8921148915290686
1107	7	0.6260579954533867
1107	8	0.7161755748846588
1107	9	0.80501094099683
1107	10	1.0242815987352287
1107	11	0.9485608482939227
1107	12	0.9136703895897682
1107	13	0.7281824332761957
1107	14	1.0126567359492427
1107	15	0.8684999724778066
1107	16	0.9352518040918056
1107	17	1.1315802132341162
1107	18	1.2137903413579068
1107	19	0.994477071667653
1107	20	1.0582239524510513
1107	21	0.864475520062129
1107	22	0.9901610144335748
1107	23	1.1552381050761227
1107	24	0.9827558039935624
1107	25	1.1666585339841546
1108	1	0.7334431626118416
1108	2	0.6326662265732139
1108	3	0.5971645767057225
1108	4	0.8112542729556367
1108	5	0.62154441346879
1108	6	0.6286730659315722
1108	7	0.5859533913641499
1108	8	0.6528806920017445
1108	9	0.7197370525907374
1108	10	0.7410416988058159
1108	11	0.4600070367266582
1108	12	0.7058592995772415
1108	13	0.711787542763318
1108	14	0.7706049914891935
1108	15	0.7537440776400359
1108	16	0.5350415146550986
1108	17	0.8657982557648083
1108	18	0.8338576045140655
1108	19	0.7930110773082646
1108	20	0.7832151654907449
1108	21	0.812791515971135
1108	22	0.8775529042464143
1108	23	1.1647042567599755
1108	24	1.0136009879679833
1108	25	1.1698588081623384
1109	1	0.7143352277083643
1109	2	0.7878371984687753
1109	3	0.8014003276074639
1109	4	0.8662736514337723
1109	5	0.6988302947358562
1109	6	0.8031358194735176
1109	7	0.6598299539569226
1109	8	0.6237770138681853
1109	9	0.7452067259623973
1109	10	0.7748722871683569
1109	11	0.8024225013738615
1109	12	0.668770344794062
1109	13	0.8025410690943815
1109	14	0.8920294232299213
1109	15	0.7969035989081377
1109	16	0.46284995833597264
1109	17	0.8851878845783787
1109	18	0.8280454549065794
1109	19	0.8961116059338607
1109	20	0.772718060066417
1109	21	0.7364956752005369
1109	22	0.7378042906236928
1109	23	1.0134981858868457
1109	24	0.7402140514847346
1109	25	0.4248393265404573
1110	1	0.6819444752574313
1110	2	0.683999372366106
1110	3	0.5846279657594629
1110	4	0.5604428580675105
1110	5	0.41410640012151845
1110	6	0.5542354603885116
1110	7	0.37555419146336533
1110	8	0.4861880391597211
1110	9	0.7265825536413217
1110	10	0.575638042757177
1110	11	0.6333549003193113
1110	12	0.5655101663743096
1110	13	0.6639438315964981
1110	14	0.7471071642909379
1110	15	0.6260016768089187
1110	16	0.6508722101853976
1110	17	0.7869149518412257
1110	18	0.3011515967028089
1110	19	0.6776170595183189
1110	20	0.7390489793620887
1110	21	0.894821484493491
1110	22	0.785768220470672
1110	23	0.8658530656994401
1110	24	0.6812669536953848
1110	25	0.9402149892560061
1111	1	0.2586136239498771
1111	2	0.2596641807200122
1111	3	0.36453381797782214
1111	4	0.24576435681810693
1111	5	0.3399906405677342
1111	6	0.3077705050506552
1111	7	0.11423324069603069
1111	8	0.2557456586582063
1111	9	0.22853331128685284
1111	10	0.2590800789953036
1111	11	-0.05462901113227048
1111	12	-0.2555836688003481
1111	13	0.005172640397411499
1111	14	0.12321662231011032
1111	15	-0.07984320053684257
1111	16	0.09639877147716902
1111	17	-0.34273378079056016
1111	18	0.05372125750581347
1111	19	0.14151879858741268
1111	20	0.15749150886701102
1111	21	0.11671895567899782
1111	22	-0.17202970005838125
1111	23	0.03215083974940569
1111	24	0.0483584301950728
1111	25	0.23342683295360445
1112	1	0.3871549825493261
1112	2	0.3073844680213007
1112	3	0.3483753122848512
1112	4	0.455651499134774
1112	5	0.3807841258970939
1112	6	0.08309851487373265
1112	7	0.0940277010349817
1112	8	0.2363408134307008
1112	9	0.17819061719541907
1112	10	0.2723247012026988
1112	11	0.2239318483386512
1112	12	0.335240816084756
1112	13	0.2996752839055148
1112	14	0.2596606759442679
1112	15	0.014382944467406844
1112	16	0.2968317118912225
1112	17	0.5080162195322444
1112	18	0.2382047345013376
1112	19	0.40022635055218814
1112	20	0.048489758443778896
1112	21	0.1029815618421992
1112	22	0.027269478241016998
1112	23	0.12931356658762638
1112	24	0.15747421603330025
1112	25	0.020631787554528103
1113	1	0.032789760282160074
1113	2	0.31154529723552926
1113	3	0.31958105041837825
1113	4	0.3409696074055404
1113	5	0.26123741641147546
1113	6	-0.18264821861942693
1113	7	-0.05243439169993991
1113	8	0.06457768080707638
1113	9	0.043283391580345665
1113	10	0.014421941649835901
1113	11	0.04918668191370301
1113	12	-0.17350385210129105
1113	13	-0.02018196160622665
1113	14	0.42862306466269473
1113	15	0.07429740523479467
1113	16	0.009723807816186536
1113	17	0.19998125401432532
1113	18	0.03617030010937583
1113	19	-0.3680329540181262
1113	20	-0.09014542051113494
1113	21	0.17515302837468738
1113	22	-0.4417389934646039
1113	23	-4.133768904282665
1113	24	-1.6353946780736663
1113	25	-0.8196657909715412
1114	1	-0.7898711680577968
1114	2	-0.995815902726715
1114	3	-0.7407579006371741
1114	4	-0.7485724538475544
1114	5	-0.5519097402939579
1114	6	-1.438262008082443
1114	7	-1.231997436386525
1114	8	-0.9612021845353915
1114	9	-1.203547244756912
1114	10	-0.8088321772557094
1114	11	-0.9789222484118554
1114	12	-2.0593018867724595
1114	13	-1.2072550391881265
1114	14	-2.8041831483912567
1114	15	-1.7368508194321421
1114	16	-1.8699323557724412
1114	17	-1.7060888224384385
1114	18	-1.6014689194222598
1114	19	-1.833675094275904
1114	20	-1.2364642306167397
1114	21	-1.1798969787916676
1114	22	-1.4347356305219918
1114	23	-5.431977490623243
1114	24	-2.71003072343494
1114	25	-2.604508690513242
1115	1	-1.2006282723192818
1115	2	-1.1724538939500917
1115	3	-0.8599655610452928
1115	4	-0.8333093883071747
1115	5	-0.8680245546069507
1115	6	-1.0273510500353638
1115	7	-1.4090941014317302
1115	8	-1.6018218791526557
1115	9	-2.804014433104893
1115	10	-3.68119632058702
1115	11	-2.3691042593652405
1115	12	-2.5513638310608044
1115	13	-1.8726916900029664
1115	14	-2.722814560477296
1115	15	-2.2067859535507353
1115	16	-2.339530018497996
1115	17	-2.160716023165797
1115	18	-4.825007882264131
1115	19	-2.8482025787961263
1115	20	-3.0371781181050537
1115	21	-2.6144483336008584
1115	22	-2.060290536999176
1115	23	-2.6800483581349965
1115	24	-2.382841398133394
1115	25	-2.6705897058593813
1116	1	-1.528284054990639
1116	2	-1.35210955264618
1116	3	-1.8062036865392557
1116	4	-1.5200603064989942
1116	5	-1.506331362398793
1116	6	-3.9602176681450487
1116	7	-2.5053763931145383
1116	8	-2.7858817592065854
1116	9	-4.027589879803276
1116	10	-3.2607311033978483
1116	11	-2.753501972021567
1116	12	-2.7091773722593366
1116	13	-4.013338530958194
1116	14	-7.0544941154060865
1116	15	-4.983573134508138
1116	16	-3.6909100181880596
1116	17	-4.772045038027336
1116	18	-3.4738456141366214
1116	19	-3.183979516297093
1116	20	-3.9314721564444035
1116	21	-4.75613889133459
1116	22	-4.314373197508409
1116	23	-3.3582170884732925
1116	24	-4.472408104575461
1116	25	-4.132541696437261
1201	1	0.6926305855820445
1201	2	0.8029131877084232
1201	3	1.0787001229768336
1201	4	1.007025036714161
1201	5	0.5685196700376238
1201	6	1.0288916882340047
1201	7	1.0358099967224277
1201	8	0.9526690194782859
1201	9	1.5886413016412462
1201	10	1.353475740597645
1201	11	1.5403366484779895
1201	12	1.4346361162975256
1201	13	1.4796845174653868
1201	14	1.5477211437366734
1201	15	1.5962759116941
1201	16	1.5556465003082423
1201	17	1.8086905171859229
1201	18	1.5366445398167983
1201	19	1.7059867582572181
1201	20	1.5722214284245482
1201	21	1.9464719932287196
1201	22	1.6927188745797324
1201	23	1.992959248586594
1201	24	1.8517400441732121
1201	25	1.9817105471966556
1202	1	1.0105778080742809
1202	2	1.0546809240893538
1202	3	1.026993563821616
1202	4	1.229506008514008
1202	5	1.024611934243005
1202	6	1.287117903562411
1202	7	1.2997731391797345
1202	8	1.3193393120334846
1202	9	1.4928750279591796
1202	10	1.4233773941571073
1202	11	1.3121434364499152
1202	12	1.5886809106787823
1202	13	1.4662868593265017
1202	14	1.9191633370443597
1202	15	1.6188483793195445
1202	16	1.5272376636321496
1202	17	1.7738104723791963
1202	18	1.7968415412841239
1202	19	1.824463669061906
1202	20	1.56184992142812
1202	21	1.6579272551644522
1202	22	1.7071361788627542
1202	23	1.8327438862368908
1202	24	1.7932121857772785
1202	25	2.096452561370022
1203	1	0.7913806627650004
1203	2	0.8857962136330748
1203	3	0.8230666493910448
1203	4	1.0282675499675804
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2211	18	-0.013533438055489455
2211	19	0.06625860784495785
2211	20	0.02114362947421
2211	21	0.1698174183326273
2211	22	0.20221767408821023
2211	23	0.39022685585857175
2211	24	0.11600738936871835
2211	25	0.5384395306646894
2212	1	-0.13627127294298091
2212	2	-0.08392691805125096
2212	3	-0.4033077343438656
2212	4	-0.06644216236360023
2212	5	-0.31109870382971394
2212	6	-0.4678304364485726
2212	7	-0.33437652274674434
2212	8	-0.3259880888503446
2212	9	-0.19777744042195167
2212	10	-0.4274015705267118
2212	11	-0.6251406324054969
2212	12	-0.19169428301459135
2212	13	-0.1949184195369753
2212	14	-0.25870848958710013
2212	15	-0.5902096786072946
2212	16	-0.4947397908006792
2212	17	-0.2767795028571811
2212	18	-0.09375844819334134
2212	19	-0.312115092840223
2212	20	-0.12057446124251214
2212	21	-0.033066155173607115
2212	22	0.1265121748249527
2212	23	0.14574301916829313
2212	24	0.03883530253369827
2212	25	0.14800160306154453
2213	1	-0.17192443572611538
2213	2	-0.17060635303236182
2213	3	-0.36196422917395665
2213	4	-0.20996202822542642
2213	5	-0.1175454827834912
2213	6	-0.05932988361459479
2213	7	-0.2203174137727828
2213	8	-0.04505976170686665
2213	9	-0.48750496935532794
2213	10	-0.22869102467620905
2213	11	-0.2562918867397883
2213	12	-0.3109520129990244
2213	13	-0.3078116158315112
2213	14	-0.38251827651313874
2213	15	-0.3250545495172332
2213	16	-0.2505948840765839
2213	17	-0.2644383739187077
2213	18	-0.21273701293104352
2213	19	-0.1755890396350921
2213	20	0.05443654625762662
2213	21	-0.23882229666439514
2213	22	-0.39838162666512034
2213	23	-0.24549289889772297
2213	24	0.013666458185241481
2213	25	0.25603623922388863
2214	1	-2.8233079994179597
2214	2	-1.5088576865061754
2214	3	-1.6891314846370484
2214	4	-3.6359467269739447
2214	5	-1.9430961477251678
2214	6	-1.5349784622039095
2214	7	-1.3048615958715644
2214	8	-1.1722736343078743
2214	9	-1.0875081477965551
2214	10	-1.0414446377986977
2214	11	-1.142467197785578
2214	12	-0.8981856566816333
2214	13	-1.3423664221643747
2214	14	-1.184328045628817
2214	15	-1.5387295280038984
2214	16	-1.5115143011740102
2214	17	-3.493654605629427
2214	18	-3.3048890001042643
2214	19	-2.133585791739044
2214	20	-2.0386926644412355
2214	21	-1.8466810808753067
2214	22	-2.720545044454738
2214	23	-1.7410675080965987
2214	24	-2.942787719875742
2214	25	-2.8778673692486585
2215	1	-1.9487302790686662
2215	2	-3.6260948758626093
2215	3	-2.177784946374416
2215	4	-3.736297472103594
2215	5	-2.3630738019855997
2215	6	-2.258732072270753
2215	7	-2.324340916430682
2215	8	-1.7944214036607242
2215	9	-4.061048895430467
2215	10	-2.5455806741564615
2215	11	-2.118400777001078
2215	12	-2.0668881367919987
2215	13	-2.0853529272936733
2215	14	-1.809828861366853
2215	15	-2.0018879530737976
2215	16	-1.961508151650193
2215	17	-2.4629561827229693
2215	18	-2.036457988129733
2215	19	-2.1777481154588685
2215	20	-2.0089412682029675
2215	21	-2.6658758933444666
2215	22	-2.5924483887584273
2215	23	-2.6261392797077328
2215	24	-2.4394032590556165
2215	25	-3.427080748834314
2216	1	-3.0883851002647447
2216	2	-3.207155801751906
2216	3	-4.439080754453585
2216	4	-5.051473294235542
2216	5	-4.85248993257018
2216	6	-3.675996178930177
2216	7	-5.4264835226310275
2216	8	-3.3378083304416037
2216	9	-4.116509404459968
2216	10	-2.9372963238170975
2216	11	-3.32038337044764
2216	12	-3.5218789404870527
2216	13	-3.212257802368587
2216	14	-4.221585075146255
2216	15	-3.587380376086113
2216	16	-3.9206914666302772
2216	17	-3.549383292780451
2216	18	-3.174890571686209
2216	19	-3.730868068931173
2216	20	-4.38343798085608
2216	21	-4.053770721862165
2216	22	-3.8616574474878327
2216	23	-3.9085504239170135
2216	24	-4.280320468692992
2216	25	-4.32512664474336
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	118153.0
3	3.0
4	1089.0
5	6545.0
6	8501.0
7	7938.0
8	7089.0
9	6634.0
10	5658.0
11	5222.0
12	4856.0
13	4774.0
14	4616.0
15	4616.0
16	4817.0
17	5157.0
18	5810.0
19	7019.0
20	9310.0
21	12096.0
22	15021.0
23	17915.0
24	21008.0
25	23756.0
26	25975.0
27	28629.0
28	31801.0
29	37518.0
30	45206.0
31	55849.0
32	69789.0
33	88382.0
34	113957.0
35	149992.0
36	204002.0
37	313916.0
38	891873.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.75201331501755	18.51205867163009	12.604156190044547	24.131771823307815
2	14.844486891759628	20.245212199448616	41.99040028964935	22.919900619142407
3	19.22395463460376	25.801336480747327	28.93647042701148	26.038238457637437
4	12.083376511865461	16.447827291511135	38.42560850007296	33.04318769655044
5	12.821004127007088	38.97900458636239	34.8563541823962	13.343637104234318
6	31.329093831982668	36.5724715158503	18.628451896304913	13.469982755862118
7	28.250975113701536	32.13128215547217	21.70333263493394	17.914410095892354
8	25.41921662683401	36.63458179947629	20.624226015203444	17.321975558486262
9	27.656613236123267	14.69236942759278	20.022188885747134	37.628828450536815
10	16.32448051909568	28.65533120373608	33.230691599831005	21.78949667733723
11	34.06464322874116	22.761856465211807	24.74117225749265	18.432328048554382
12	24.139170428485368	24.925543295806165	31.12766504198998	19.807621233718482
13	29.34038080741466	22.32614899591696	25.7901741155595	22.54329608110888
14	21.291311477769245	22.774702225488262	27.57253557674154	28.361450720000946
15	23.176969573830448	31.237364758470054	23.468913496215777	22.116752171483718
16	21.857675067929293	28.206645577791576	27.9752690397789	21.96041031450023
17	21.360048634854188	28.81852188653109	27.763016851164135	22.058412627450583
18	21.149760671153782	27.02193290324984	30.60170481053944	21.226601615056932
19	23.474218551229942	26.635912628276472	28.097823072979526	21.79204574751406
20	24.01586653148224	26.682781553675483	28.631215326358213	20.670136588484063
21	23.387752856072566	26.444459494351346	27.467142996220122	22.700644653355965
22	23.646689994305685	27.896553986972933	27.604725646220054	20.85203037250133
23	22.30403466631219	27.677592459162685	28.486168273517066	21.532204601008054
24	23.170563929460243	26.36093060192634	28.997768699051218	21.470736769562194
25	22.5082281923799	27.062297205483627	28.937541934678208	21.491932667458265
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	422.0
1	422.0
2	998.5
3	1575.0
4	1575.0
5	1575.0
6	2984.5
7	4394.0
8	4394.0
9	4394.0
10	5285.5
11	6177.0
12	6177.0
13	6177.0
14	8385.0
15	10593.0
16	10593.0
17	10593.0
18	19540.5
19	28488.0
20	28488.0
21	28488.0
22	44287.0
23	60086.0
24	60086.0
25	60086.0
26	88927.0
27	117768.0
28	117768.0
29	117768.0
30	151428.0
31	185088.0
32	185088.0
33	185088.0
34	205257.5
35	225427.0
36	225427.0
37	225427.0
38	238296.5
39	251166.0
40	251166.0
41	251166.0
42	265124.5
43	279083.0
44	279083.0
45	279083.0
46	294223.0
47	309363.0
48	309363.0
49	309363.0
50	297476.5
51	285590.0
52	285590.0
53	285590.0
54	254047.5
55	222505.0
56	222505.0
57	222505.0
58	192291.0
59	162077.0
60	162077.0
61	162077.0
62	135530.0
63	108983.0
64	108983.0
65	108983.0
66	83985.5
67	58988.0
68	58988.0
69	58988.0
70	43768.5
71	28549.0
72	28549.0
73	28549.0
74	20150.0
75	11751.0
76	11751.0
77	11751.0
78	8075.0
79	4399.0
80	4399.0
81	4399.0
82	2928.5
83	1458.0
84	1458.0
85	1458.0
86	934.0
87	410.0
88	410.0
89	410.0
90	261.5
91	113.0
92	113.0
93	113.0
94	69.5
95	26.0
96	26.0
97	26.0
98	19.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3406651407575073
2	0.010742265146170932
3	0.009431201289748496
4	0.01171498994287145
5	0.013068346181759127
6	0.08247014580721779
7	0.08234326865982207
8	0.1068728504896612
9	0.08661479928881129
10	0.09431201289748496
11	0.08923692700165617
12	0.10069816264973618
13	0.08619187546415889
14	0.09025194418082193
15	0.08678396881867226
16	0.08919463461919093
17	0.10150171791657574
18	0.10446218468914253
19	0.0976531111122389
20	0.09672267869800363
21	0.08382350204610546
22	0.10518115519105162
23	0.09786457302456511
24	0.09274719474627109
25	0.09249344045147964
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2364492.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.062947713983974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.92243855104888	36.006492806433855
2	14.653123406684193	14.67157101910771
3	5.4988546114652355	8.258666127017513
4	2.7311956427273785	5.469268186340864
5	1.5051951729986552	3.7677253622586364
6	0.9084841210425271	2.7288835830442038
7	0.591936262732793	2.074385191984203
8	0.4250368031384218	1.702287620163017
9	0.30092094490074145	1.3558490577547626
>10	1.337529456378793	11.438311742295024
>50	0.07178181084420654	2.491365298247613
>100	0.04541584279094995	4.363368236062885
>500	0.005278075171964856	1.910341052445857
>1k	0.0024687770965638916	2.3889917833045415
>5k	2.5539073412729913E-4	0.8432537940319982
>10k+	8.513024470909971E-5	0.5292391395071947
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	12418	0.525186805453349	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7343	0.31055296444225655	No Hit
GGTATCAACGCAGAGTACTTTTTTT	7098	0.30019133073827275	No Hit
GTCCTACAGTGGACATTTCTAAATT	5345	0.22605278427670722	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4806	0.2032571901279429	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4639	0.19619436225624784	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4276	0.18084222742136577	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3265	0.13808462874900823	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2746	0.11613488224954875	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2694	0.11393567836135628	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2562	0.10835308387594461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.229238246523989E-5	0.0
11	0.0	0.0	0.0	4.229238246523989E-5	0.0
12	0.0	0.0	0.0	4.229238246523989E-5	0.0
13	0.0	0.0	0.0	4.229238246523989E-5	4.229238246523989E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2100	0.0	14.597768	1
ATACGTA	40	0.0052841613	14.248381	6
TAGGACC	935	0.0	13.715019	4
ATATCGC	60	4.0966208E-4	12.665495	15
GCACCGT	135	2.7284841E-11	12.665227	6
TCCAACG	120	7.421477E-10	12.664959	18
ACACCGT	70	1.09252185E-4	12.212898	6
AGGACCT	1760	0.0	11.819679	5
ATCGCAA	75	2.0768763E-4	11.398705	17
CGCGCCA	60	0.005884019	11.081604	10
GGACCTG	1750	0.0	10.964468	6
CGTGCGC	130	3.2761818E-8	10.959828	10
GACGGTT	70	0.0014935782	10.8556795	18
CGCCCTA	405	0.0	10.788897	16
TGTAGGA	2925	0.0	10.749206	2
AGCACCG	115	8.8349225E-7	10.73791	5
GTATCAA	4655	0.0	10.71671	1
GTCCTAC	2605	0.0	10.708035	1
ACGGTTG	80	3.7769412E-4	10.686059	19
CTGTAGG	2825	0.0	10.581839	1
>>END_MODULE