FastQCFastQC Report
Thu 26 May 2016
SRR1512896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512896_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2364492
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT152760.6460584345390046No Hit
TATCAACGCAGAGTACTTTTTTTTT108460.4587031802179919No Hit
GGTATCAACGCAGAGTACTTTTTTT87230.3689164522442876No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51990.21987809643678216No Hit
GTCCTACAGTGGACATTTCTAAATT51070.2159871972499801No Hit
GTCCTAAAGTGTGTATTTCTCATTT45810.19374140407326393No Hit
CTGTAGGACGTGGAATATGGCAAGA45040.19048489062344046No Hit
CTTTAGGACGTGAAATATGGCGAGG44570.1884971486475742No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32540.1376194125418906No Hit
GTCCTACAGTGTGCATTTCTCATTT27040.11435860218600867No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26410.11169418209069856No Hit
GTACTGGTTCACTATCGGTCAGTCA24340.1029396589203939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22700.014.2042981
TAGGACC8850.012.8938524
GGTCGGT550.003083986212.08318211
GGCGAGG11850.011.05435119
ACTGCGT700.001491428910.8575548
TCCAACG1855.456968E-1210.77506918
TGTAGGA26150.010.7287772
AGGACCT17600.010.6978675
GTACTAG909.403597E-510.5682581
GTATCAA49600.010.5661281
GGACCTG16850.010.5530316
CCTACAC3500.010.32560353
ATCGCGC1856.002665E-1110.2706598
TGGCGAG26400.010.21143818
CTGTAGG26700.010.1882761
ACTGTGC5700.010.1670528
AACTGCG750.002634159810.1399417
GTAGGAC26650.010.1349363
CTAGGAC4600.010.130673
AGGCGTG1606.1099854E-910.1003317
Produced by FastQC (version 0.11.5)