##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512896_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2364492 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.256139162238654 31.0 30.0 34.0 26.0 34.0 2 30.264249149500188 31.0 30.0 34.0 26.0 34.0 3 30.427309544714046 31.0 30.0 34.0 26.0 34.0 4 34.00746587427659 37.0 35.0 37.0 30.0 37.0 5 33.59732153883371 37.0 35.0 37.0 28.0 37.0 6 33.44401461286399 37.0 35.0 37.0 28.0 37.0 7 33.27494362425417 37.0 35.0 37.0 27.0 37.0 8 33.21906058468373 37.0 35.0 37.0 26.0 37.0 9 34.516897921413985 39.0 35.0 39.0 26.0 39.0 10 34.449887333093116 39.0 35.0 39.0 26.0 39.0 11 34.41294705162885 39.0 35.0 39.0 26.0 39.0 12 34.27885736132751 39.0 35.0 39.0 25.0 39.0 13 34.26100024867921 39.0 35.0 39.0 25.0 39.0 14 35.339290638327384 40.0 36.0 41.0 25.0 41.0 15 35.34680514884381 40.0 36.0 41.0 25.0 41.0 16 35.35471763067923 40.0 36.0 41.0 25.0 41.0 17 35.35792423911775 40.0 36.0 41.0 25.0 41.0 18 35.29035623719598 40.0 36.0 41.0 25.0 41.0 19 35.31058426080528 40.0 36.0 41.0 25.0 41.0 20 35.28532598122557 40.0 36.0 41.0 25.0 41.0 21 35.254085021222316 40.0 36.0 41.0 25.0 41.0 22 35.21847694980571 40.0 36.0 41.0 25.0 41.0 23 35.06430852800517 40.0 35.0 41.0 24.0 41.0 24 35.02120159425365 40.0 35.0 41.0 24.0 41.0 25 34.96599142648822 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6164104963297952 1101 2 0.48209254708524085 1101 3 0.4786543383847608 1101 4 0.5492429595513499 1101 5 0.6306980722328106 1101 6 0.6867723371054382 1101 7 0.7065967132446644 1101 8 0.45018352940939366 1101 9 0.6983775395514158 1101 10 0.4714191228379292 1101 11 0.7805086396951992 1101 12 0.9313978793910564 1101 13 0.9219633010409609 1101 14 1.0128456609760192 1101 15 0.6895054466464643 1101 16 0.8994828894453448 1101 17 1.0015708848586016 1101 18 0.8008917441645877 1101 19 0.767974605708595 1101 20 0.8149446899885433 1101 21 0.840656343081676 1101 22 0.8381541292398325 1101 23 0.8290846410189019 1101 24 0.9489856610873204 1101 25 0.9989996448199747 1102 1 0.7405800693217763 1102 2 0.7571468727719477 1102 3 0.48452925997333196 1102 4 0.5863165700758515 1102 5 0.7860928097325939 1102 6 0.7197175546955918 1102 7 0.7261835599931246 1102 8 0.7842711952605015 1102 9 0.898419619309891 1102 10 0.9800080638900255 1102 11 1.0534750658584997 1102 12 1.0039029120926841 1102 13 0.9933772121655124 1102 14 0.9334442494453512 1102 15 1.0097805957843775 1102 16 1.0511724526655755 1102 17 1.1785196275266685 1102 18 0.544981678011581 1102 19 0.8822962812514774 1102 20 1.0752325973843355 1102 21 0.941868939893233 1102 22 0.7473386222708953 1102 23 0.9009057604966699 1102 24 0.9580142996899852 1102 25 1.0881805390476416 1103 1 0.6583982511951056 1103 2 0.7733683822681776 1103 3 0.6251412552852749 1103 4 0.44234291977290496 1103 5 0.5492180660348964 1103 6 0.4992347084478581 1103 7 0.6389157334852129 1103 8 0.6843503001618458 1103 9 0.7654110063391144 1103 10 0.7257545830317156 1103 11 0.7675845533509431 1103 12 0.9049757571514192 1103 13 0.9736611951215579 1103 14 0.8741261895051906 1103 15 0.9983444323478281 1103 16 0.9803347414664074 1103 17 1.020935642265414 1103 18 0.8909884274067679 1103 19 0.8154292842530921 1103 20 0.8287617588238518 1103 21 0.9066005291980588 1103 22 1.053457225632613 1103 23 0.9720249492589232 1103 24 0.8384131030463635 1103 25 0.7572947916397865 1104 1 0.68180829393037 1104 2 0.7229837231754921 1104 3 0.6113588975789028 1104 4 0.517746319202196 1104 5 0.608662726524642 1104 6 0.5133459994615635 1104 7 0.4815975147502982 1104 8 0.5398724700582491 1104 9 0.6173623471463401 1104 10 0.5902100811180659 1104 11 0.6101902264339856 1104 12 0.6479255851782071 1104 13 0.5086329047719715 1104 14 0.7325368212969323 1104 15 0.7374294243356161 1104 16 0.7344198220733134 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-2.2884584012416624 2216 18 -2.716060277420766 2216 19 -3.041528947257085 2216 20 -2.204239979164825 2216 21 -2.832920483286209 2216 22 -2.356537966142291 2216 23 -3.593729255835754 2216 24 -2.794738768092003 2216 25 -2.8567868809137806 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66644.0 3 1.0 4 1138.0 5 9530.0 6 12487.0 7 10014.0 8 8015.0 9 6555.0 10 5663.0 11 5147.0 12 4468.0 13 4106.0 14 3995.0 15 3842.0 16 4029.0 17 4173.0 18 4590.0 19 5809.0 20 7804.0 21 10254.0 22 12827.0 23 15282.0 24 18037.0 25 20463.0 26 22205.0 27 24398.0 28 27455.0 29 32746.0 30 40590.0 31 51738.0 32 65683.0 33 84333.0 34 109915.0 35 144622.0 36 199611.0 37 310124.0 38 1006199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.776363285429845 19.319813352476668 13.601955193758549 26.301868168334934 2 13.320823531402848 20.703369607527883 41.01574796486537 24.9600588962039 3 17.306456641220016 25.74928162955562 29.75155571140988 27.192706017814487 4 11.332517598016182 17.008546610898907 37.54989259109033 34.10904319999459 5 12.466275751620328 39.17266561366076 34.8836687921368 13.477389842582104 6 31.353275533452724 37.19908120533402 18.07569392017929 13.371949341033964 7 27.8605206899135 32.2445382835458 21.714854319662518 18.180086706878186 8 25.464168533439853 36.941459629037546 20.302776929137657 17.29159490838495 9 27.118603200118446 14.602734800494929 19.93405175604649 38.344610243340135 10 15.737559804462101 29.199344821939576 33.20594230083268 21.85715307276564 11 33.89109694403007 22.705738598321805 24.696583334848917 18.70658112279921 12 23.730305981804932 25.227755534287144 31.168536769286533 19.873401714621398 13 29.071515789268886 22.234525298400115 25.985055568972392 22.708903343358603 14 21.00022203660432 22.6152105647131 27.741972319436663 28.64259507924592 15 22.985740293315466 31.23841018144411 23.59183704545916 22.184012479781266 16 21.811034479258222 28.166083115999836 27.901983790290586 22.12089861445136 17 21.031379817001117 28.874473119025406 27.956137449638085 22.13800961433539 18 20.969875787956404 26.90932453145566 30.849402624998728 21.271397055589205 19 23.186029417362317 26.51694640707749 28.49888388608956 21.798140289470634 20 23.78717909786857 26.559812422842015 28.909432865842493 20.74357561344692 21 23.007771661349096 26.21819184533819 27.956665459921982 22.81737103339074 22 23.355582021278217 27.638588376998957 28.03761835563277 20.968211246090057 23 21.957359128303246 27.334201173021523 28.94955872128136 21.758880977393876 24 23.034292355398115 26.237898034757574 29.28197684745814 21.445832762386168 25 22.202114535432784 26.916279233035777 29.320306146227665 21.561300085303774 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 195.0 1 195.0 2 589.5 3 984.0 4 984.0 5 984.0 6 2572.5 7 4161.0 8 4161.0 9 4161.0 10 5133.0 11 6105.0 12 6105.0 13 6105.0 14 8395.0 15 10685.0 16 10685.0 17 10685.0 18 19758.0 19 28831.0 20 28831.0 21 28831.0 22 45663.5 23 62496.0 24 62496.0 25 62496.0 26 92571.5 27 122647.0 28 122647.0 29 122647.0 30 155716.5 31 188786.0 32 188786.0 33 188786.0 34 207021.0 35 225256.0 36 225256.0 37 225256.0 38 238195.0 39 251134.0 40 251134.0 41 251134.0 42 266174.0 43 281214.0 44 281214.0 45 281214.0 46 294639.0 47 308064.0 48 308064.0 49 308064.0 50 295824.0 51 283584.0 52 283584.0 53 283584.0 54 252540.0 55 221496.0 56 221496.0 57 221496.0 58 190919.0 59 160342.0 60 160342.0 61 160342.0 62 133485.5 63 106629.0 64 106629.0 65 106629.0 66 82170.0 67 57711.0 68 57711.0 69 57711.0 70 42435.5 71 27160.0 72 27160.0 73 27160.0 74 19149.0 75 11138.0 76 11138.0 77 11138.0 78 7607.5 79 4077.0 80 4077.0 81 4077.0 82 2719.5 83 1362.0 84 1362.0 85 1362.0 86 850.0 87 338.0 88 338.0 89 338.0 90 213.0 91 88.0 92 88.0 93 88.0 94 47.5 95 7.0 96 7.0 97 7.0 98 4.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08306223916173114 2 0.04279989105482277 3 0.01949678831647559 4 0.006893658341834103 5 0.019708250228801787 6 0.059040165921474895 7 0.07692984370427136 8 0.05218879996210603 9 0.021865161734529025 10 0.04927062557200447 11 0.007147412636625542 12 2.1146191232619945E-4 13 0.005624886867876906 14 7.189705019090781E-4 15 0.014717749097903481 16 0.009727247967005175 17 0.004482992541315428 18 0.006259272604855505 19 0.0016071105336791158 20 0.0010573095616309973 21 1.6916952986095954E-4 22 3.383390597219191E-4 23 0.0 24 0.0 25 4.2292382465239886E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2364492.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.525332346165335 #Duplication Level Percentage of deduplicated Percentage of total 1 70.39639368724168 34.16008399644898 2 15.362232246759605 14.909148507059761 3 5.804335624168607 8.44971945634406 4 2.943240084337853 5.712868130682001 5 1.5893510237058313 3.856189332002178 6 0.9722523612622489 2.830732137275678 7 0.6382228020286931 2.1678981505540253 8 0.43429766197041564 1.6859550707421591 9 0.32794946102325734 1.4322470930009423 >10 1.3977543777066426 11.536826468676317 >50 0.0770067181677567 2.560946765962644 >100 0.048657696560250735 4.539036920932573 >500 0.005071187304091624 1.755321581464803 >1k 0.0027978964436365925 2.4873702754266414 >5k 2.6230279159093056E-4 0.8073579806074704 >10k+ 1.7486852772728703E-4 1.1082981328198194 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 15276 0.6460584345390046 No Hit TATCAACGCAGAGTACTTTTTTTTT 10846 0.4587031802179919 No Hit GGTATCAACGCAGAGTACTTTTTTT 8723 0.3689164522442876 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 5199 0.21987809643678216 No Hit GTCCTACAGTGGACATTTCTAAATT 5107 0.2159871972499801 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4581 0.19374140407326393 No Hit CTGTAGGACGTGGAATATGGCAAGA 4504 0.19048489062344046 No Hit CTTTAGGACGTGAAATATGGCGAGG 4457 0.1884971486475742 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3254 0.1376194125418906 No Hit GTCCTACAGTGTGCATTTCTCATTT 2704 0.11435860218600867 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2641 0.11169418209069856 No Hit GTACTGGTTCACTATCGGTCAGTCA 2434 0.1029396589203939 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 4.229238246523989E-5 9 0.0 0.0 0.0 0.0 8.458476493047979E-5 10 0.0 0.0 0.0 0.0 8.458476493047979E-5 11 0.0 0.0 0.0 0.0 8.458476493047979E-5 12 0.0 0.0 0.0 0.0 8.458476493047979E-5 13 0.0 0.0 0.0 0.0 8.458476493047979E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2270 0.0 14.204298 1 TAGGACC 885 0.0 12.893852 4 GGTCGGT 55 0.0030839862 12.083182 11 GGCGAGG 1185 0.0 11.054351 19 ACTGCGT 70 0.0014914289 10.857554 8 TCCAACG 185 5.456968E-12 10.775069 18 TGTAGGA 2615 0.0 10.728777 2 AGGACCT 1760 0.0 10.697867 5 GTACTAG 90 9.403597E-5 10.568258 1 GTATCAA 4960 0.0 10.566128 1 GGACCTG 1685 0.0 10.553031 6 CCTACAC 350 0.0 10.3256035 3 ATCGCGC 185 6.002665E-11 10.270659 8 TGGCGAG 2640 0.0 10.211438 18 CTGTAGG 2670 0.0 10.188276 1 ACTGTGC 570 0.0 10.167052 8 AACTGCG 75 0.0026341598 10.139941 7 GTAGGAC 2665 0.0 10.134936 3 CTAGGAC 460 0.0 10.13067 3 AGGCGTG 160 6.1099854E-9 10.100331 7 >>END_MODULE