FastQCFastQC Report
Thu 26 May 2016
SRR1512895_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512895_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507190
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72910.29080364870632064No Hit
GTCCTACAGTGGACATTTCTAAATT43890.1750565373984421No Hit
TATCAACGCAGAGTACTTTTTTTTT41900.16711936470710237No Hit
GGTATCAACGCAGAGTACTTTTTTT41060.1637690003549791No Hit
CTGTAGGACGTGGAATATGGCAAGA36670.14625935808614426No Hit
GTCCTAAAGTGTGTATTTCTCATTT35830.14290899373402094No Hit
CTTTAGGACGTGAAATATGGCGAGG27550.10988397369166279No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATA508.7318804E-515.19702911
GGTATCA19300.014.119511
TAGGACC7950.012.6646984
ACACCGT1103.8258804E-812.0890286
CTACACT3200.011.8731534
GTACTAG1604.3655746E-1111.3147381
TGTAGGA22300.011.0745112
GTCCTAC19750.011.0478881
ATACGGC1053.474839E-610.85545515
GCCGGTT1053.4765017E-610.855020511
GCGGGAT700.00149438910.855020511
CTGTAGG21250.010.8509391
GTATAGA3350.010.8081081
CCTACAC3200.010.6858383
GTAGGAC21000.010.674533
GTCCTAA10900.010.5771771
TCCTACA22600.010.4651862
GGACCGT1002.4041043E-510.4483746
GGACGTG20050.010.4223186
ATTTAGA4950.010.3944011
Produced by FastQC (version 0.11.5)