FastQCFastQC Report
Thu 26 May 2016
SRR1512895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507190
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT94440.3766766778744331No Hit
TATCAACGCAGAGTACTTTTTTTTT64910.2588954167813369No Hit
GGTATCAACGCAGAGTACTTTTTTT55570.22164255600891836No Hit
GTCCTACAGTGGACATTTCTAAATT41130.1640481973843227No Hit
CTGTAGGACGTGGAATATGGCAAGA36450.14538188170820718No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34140.13616837973986815No Hit
GTCCTAAAGTGTGTATTTCTCATTT33830.134931935752775No Hit
CTTTAGGACGTGAAATATGGCGAGG28140.11223720579613032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.014.1761341
TAGGACC7800.012.9248814
TAGCACG550.0030434312.1046074
GTCCTAC19550.011.4364871
GTCCTAA10350.011.1228631
TGTAGGA22300.011.0481192
AGGACCT13950.010.9765785
GTAGGAC20300.010.9651163
TAGGACT2450.010.8694424
CTTACAC3250.010.8295853
TCCTACA22950.010.7766582
CGTCTGA909.607148E-510.54601619
GTATCAA44550.010.5072471
GTTTAGG2550.010.4469471
CTGTAGG22000.010.4223561
CTAGTAC1303.7753307E-710.2442023
ATTTCTA14200.010.22748115
TCGAAAC2151.8189894E-1210.15421316
GGACCTG13850.010.0944556
CCGATCA856.6084403E-410.0567539
Produced by FastQC (version 0.11.5)