##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512895_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2507190
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.272590031070642	31.0	30.0	34.0	26.0	34.0
2	30.287151352709607	31.0	30.0	34.0	26.0	34.0
3	30.46183695691192	31.0	31.0	34.0	26.0	34.0
4	34.05198927883407	37.0	35.0	37.0	30.0	37.0
5	33.62889888680156	37.0	35.0	37.0	28.0	37.0
6	33.49928166592879	37.0	35.0	37.0	28.0	37.0
7	33.33411029878071	37.0	35.0	37.0	27.0	37.0
8	33.283062312788424	37.0	35.0	37.0	27.0	37.0
9	34.62316019128985	39.0	35.0	39.0	27.0	39.0
10	34.5485316230521	39.0	35.0	39.0	27.0	39.0
11	34.52779366541826	39.0	35.0	39.0	27.0	39.0
12	34.38957159210112	39.0	35.0	39.0	26.0	39.0
13	34.37752942537263	39.0	35.0	39.0	26.0	39.0
14	35.454190946836896	40.0	36.0	41.0	26.0	41.0
15	35.448709910297985	40.0	36.0	41.0	26.0	41.0
16	35.44631559634491	40.0	36.0	41.0	26.0	41.0
17	35.42946924644722	40.0	36.0	41.0	26.0	41.0
18	35.36275910481455	40.0	36.0	41.0	25.0	41.0
19	35.37721313502367	40.0	36.0	41.0	25.0	41.0
20	35.34420167597988	40.0	36.0	41.0	25.0	41.0
21	35.31327940842138	40.0	36.0	41.0	25.0	41.0
22	35.273213837004775	40.0	36.0	41.0	25.0	41.0
23	35.1201624129005	40.0	35.0	41.0	25.0	41.0
24	35.0723511181841	40.0	35.0	41.0	24.0	41.0
25	35.01119939055277	40.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5581823185198793
1101	2	0.37476922403831736
1101	3	0.4417729069786276
1101	4	0.4561069462950016
1101	5	0.5207022146943601
1101	6	0.5084851942988635
1101	7	0.5963740221973097
1101	8	0.33538992502949583
1101	9	0.749762298950202
1101	10	0.40118048678923657
1101	11	0.7187007085888055
1101	12	0.7749369157102706
1101	13	0.8476931034159421
1101	14	0.9833341364438866
1101	15	0.7016836920325318
1101	16	0.8835808533992449
1101	17	1.0080033980167826
1101	18	0.8584709448283689
1101	19	0.7141350237806847
1101	20	0.7523017087265131
1101	21	0.7842323441341534
1101	22	0.7672971001284736
1101	23	0.8567297811675942
1101	24	0.9482488635835082
1101	25	1.104481806549117
1102	1	0.849724588985989
1102	2	0.9521290625362937
1102	3	0.6857701926031794
1102	4	0.8184416410100326
1102	5	0.9145351818847303
1102	6	0.8285443071798042
1102	7	0.9343594611879453
1102	8	0.9888864600943919
1102	9	1.147898903434097
1102	10	0.9913662055408423
1102	11	1.1130310070995861
1102	12	1.125484567779658
1102	13	1.282417849126439
1102	14	1.2087757236566787
1102	15	1.2729645987821527
1102	16	1.3504773497229081
1102	17	1.2898110680907564
1102	18	0.7083825188495041
1102	19	1.1089094224307985
1102	20	1.267488011638477
1102	21	1.1924734020216121
1102	22	1.0985378719501782
1102	23	1.333744833701246
1102	24	1.2680591034100246
1102	25	1.3537245065839443
1103	1	0.6522904820604296
1103	2	0.869058417263556
1103	3	0.5826063219485427
1103	4	0.46211164476294186
1103	5	0.6422537023209216
1103	6	0.5362670501367148
1103	7	0.596193155333502
1103	8	0.648985115951902
1103	9	0.662959094309592
1103	10	0.6379466089738273
1103	11	0.7559419149721407
1103	12	0.7479163831828615
1103	13	0.7833194251483775
1103	14	0.725215683709024
1103	15	0.8687486366693591
1103	16	0.8627282472746316
1103	17	0.7700733778894104
1103	18	0.8598660484245073
1103	19	0.8666222077419619
1103	20	0.8391316431286882
1103	21	0.8568740668225132
1103	22	0.7764157479770262
1103	23	0.7804388354469225
1103	24	0.838093480901513
1103	25	0.8065615176831571
1104	1	0.638672862475012
1104	2	0.7663908740005994
1104	3	0.6998952960379619
1104	4	0.5429203048399458
1104	5	0.6796704555080737
1104	6	0.6469554494480221
1104	7	0.5981341217809089
1104	8	0.6165440665099169
1104	9	0.6784335140798774
1104	10	0.7097856060088077
1104	11	0.7719287530090426
1104	12	0.7607716894653933
1104	13	0.6009080910187308
1104	14	0.8678877184430647
1104	15	0.8691167420171411
1104	16	0.8465415713212536
1104	17	0.7121606751629557
1104	18	0.3175899140839604
1104	19	0.8098946024446292
1104	20	0.7461363201170599
1104	21	0.8375200575336308
1104	22	0.7716657894743122
1104	23	0.73229039558219
1104	24	0.9521900605486451
1104	25	0.8888189701077422
1105	1	0.5252996515535955
1105	2	0.6019313704345599
1105	3	0.5905639404261755
1105	4	0.42740087824993367
1105	5	0.469754767199575
1105	6	0.5128284422383871
1105	7	0.5067902729150333
1105	8	0.5362764924180752
1105	9	0.8353828462756994
1105	10	0.5653509209092604
1105	11	0.8241111299588937
1105	12	0.8851747697990149
1105	13	0.8880884482366014
1105	14	0.9793703956058764
1105	15	0.9661485439089006
1105	16	0.8206195879121339
1105	17	0.6209426636779511
1105	18	0.5744284033890779
1105	19	0.848925518885892
1105	20	0.777206778063011
1105	21	0.7153214257808571
1105	22	0.6009621148547453
1105	23	0.8057025792517649
1105	24	0.8018454781161068
1105	25	0.7146629433891079
1106	1	0.016956401823016165
1106	2	0.3170632207192483
1106	3	-1.5803364395194173
1106	4	-0.4401609528041135
1106	5	-0.19457028868657034
1106	6	-0.011869730501899767
1106	7	0.18051772995635673
1106	8	0.2296162626994871
1106	9	0.48009164471336874
1106	10	0.19927329546995765
1106	11	0.34968596874923463
1106	12	0.45424305180308266
1106	13	0.4062327271163255
1106	14	0.27822457677572743
1106	15	0.43852731833555225
1106	16	0.4656470126503933
1106	17	0.44396215503547154
1106	18	0.5639782890136047
1106	19	0.5127168576409389
1106	20	0.3703659561899002
1106	21	0.42588739470391346
1106	22	0.42612239104925465
1106	23	0.4839664675654589
1106	24	0.5649280557719862
1106	25	0.5045914467237438
1107	1	0.5264641116831683
1107	2	0.6471571592160288
1107	3	0.5122311832351301
1107	4	0.40229136728301995
1107	5	0.44716973851421926
1107	6	0.482487458890283
1107	7	0.4366668529444624
1107	8	0.34685333511315264
1107	9	0.2550005790064276
1107	10	0.35451158829039287
1107	11	0.41728840027958114
1107	12	0.6074032680810078
1107	13	0.4842448386085252
1107	14	0.5242222567462989
1107	15	0.5576725745651956
1107	16	0.5601285891625807
1107	17	0.6320602014742676
1107	18	0.5220825503206683
1107	19	0.4891099747929317
1107	20	0.568881760508873
1107	21	0.4791255564825647
1107	22	0.4230043145785487
1107	23	0.603011455031222
1107	24	0.650227924127563
1107	25	0.690690528931178
1108	1	0.43087503512927583
1108	2	0.3819043932976989
1108	3	0.4544428275704
1108	4	0.28178274536259096
1108	5	0.440860002599031
1108	6	0.45927198226441845
1108	7	0.457984641657049
1108	8	0.394822118422006
1108	9	0.502695239615484
1108	10	0.5196776656246271
1108	11	0.45786604058992
1108	12	0.5812178640330004
1108	13	0.6235044506269958
1108	14	0.609970369937237
1108	15	0.6581980843801247
1108	16	0.5467611654905795
1108	17	0.5706890354761427
1108	18	0.6470082318503785
1108	19	0.598007313604974
1108	20	0.6688143116077114
1108	21	0.6357942701667412
1108	22	0.577097551011029
1108	23	0.6972850457672308
1108	24	0.7533848945155626
1108	25	0.716804670367047
1109	1	0.2851633620187002
1109	2	0.21772175893092083
1109	3	0.38231125718350967
1109	4	0.3119984905796258
1109	5	-0.032076068464903074
1109	6	0.2601196682379907
1109	7	0.36881214836387244
1109	8	0.31960903339963664
1109	9	0.6261233732072284
1109	10	0.13889688624147567
1109	11	0.44471914297459136
1109	12	0.6408683203323235
1109	13	0.5519285571837145
1109	14	0.6463976452120264
1109	15	0.5735617618959452
1109	16	0.6008198240078073
1109	17	0.6468281670867029
1109	18	0.7251236480459227
1109	19	0.6152655102379398
1109	20	0.7261195833982796
1109	21	0.7655426931563127
1109	22	0.790540450397863
1109	23	0.6939486570476276
1109	24	0.6933350415856268
1109	25	0.6140221949260862
1110	1	0.33786050259771017
1110	2	0.26678642259366825
1110	3	0.341486933546598
1110	4	0.3762643320561878
1110	5	0.43173290528278585
1110	6	0.43170853644682694
1110	7	0.32839574668827964
1110	8	0.463597088782592
1110	9	0.45420592823958117
1110	10	0.6579379094149047
1110	11	0.5840477365273316
1110	12	0.6375966693752773
1110	13	0.5833748133436174
1110	14	0.6296660298727019
1110	15	0.6476608105080217
1110	16	0.5182548126621782
1110	17	0.6079325890240455
1110	18	0.6091539413627416
1110	19	0.7189465678364755
1110	20	0.7220016647446386
1110	21	0.7779407461321384
1110	22	0.7758361536604355
1110	23	0.8397903721306932
1110	24	0.7553883445243912
1110	25	0.7567485155551807
1111	1	0.21250560674662466
1111	2	0.0888202653454897
1111	3	3.677424396428819E-4
1111	4	0.23291954882490273
1111	5	0.3553842282121522
1111	6	0.3571184966926637
1111	7	0.2851909552118954
1111	8	0.34183995189803795
1111	9	0.3599023253092213
1111	10	0.36989176312133054
1111	11	0.33768555556941493
1111	12	0.5218445378150705
1111	13	0.43831036032388937
1111	14	0.28214414614598127
1111	15	0.13753799360004848
1111	16	0.12391763210486317
1111	17	0.32960137523289035
1111	18	0.132427024342455
1111	19	-0.06843895139309097
1111	20	0.3446423083862271
1111	21	0.39613799903066393
1111	22	0.08487123026258558
1111	23	0.14221517208524403
1111	24	0.31751933660123655
1111	25	0.23484730807166443
1112	1	0.27524262406208067
1112	2	-0.18183078456791435
1112	3	0.212100930337197
1112	4	0.36083412876180176
1112	5	0.238043797065437
1112	6	0.18185103364781696
1112	7	0.3562059855305648
1112	8	0.3300987982639043
1112	9	0.4226557139237883
1112	10	0.069825886968502
1112	11	0.16710517102470135
1112	12	0.5545380221357661
1112	13	0.3468246087297615
1112	14	0.1712351920716273
1112	15	-0.09377820020485927
1112	16	-0.12814002028017057
1112	17	0.15245781017969762
1112	18	0.33277898283851925
1112	19	0.26401521874331024
1112	20	0.1379348649673915
1112	21	0.29019150336832666
1112	22	0.32561533757229455
1112	23	0.5199135042652543
1112	24	0.47818726605706274
1112	25	0.34625131068325743
1113	1	-0.5114867742911784
1113	2	-0.3732664283787095
1113	3	-0.45675441757849455
1113	4	-2.1065683761913583
1113	5	-0.7688247874767313
1113	6	-0.32463722811779405
1113	7	-0.48437418093531903
1113	8	-0.3622478281137731
1113	9	-0.24223262848618532
1113	10	-0.31906709498110075
1113	11	-0.43261070695341175
1113	12	-0.057084846015683866
1113	13	-0.17914934612930722
1113	14	-0.3813949589623107
1113	15	-0.3965156281476325
1113	16	-0.653640687338445
1113	17	-0.6694093805093644
1113	18	-0.42335571315215503
1113	19	-0.19648124186540628
1113	20	-0.06923501585816894
1113	21	-0.15597253820832435
1113	22	-0.16597126550811936
1113	23	0.03967796798026768
1113	24	-1.3806921438686004E-4
1113	25	-0.29407534874864893
1114	1	-1.0696360039866022
1114	2	-2.3063859398887097
1114	3	-1.6114517986284547
1114	4	-1.1759089449517077
1114	5	-1.03592394392102
1114	6	-0.6585005659091294
1114	7	-0.8526910757977433
1114	8	-0.8164545877428253
1114	9	-1.0382126641808966
1114	10	-1.1827111793353566
1114	11	-1.183173658523458
1114	12	-1.184723317629313
1114	13	-1.188427735301694
1114	14	-1.1900234622380594
1114	15	-1.3317026833205006
1114	16	-1.7261204964052652
1114	17	-1.7974822277253253
1114	18	-1.4791763001743092
1114	19	-1.9653123748140757
1114	20	-1.3265758845962168
1114	21	-1.6476492753982512
1114	22	-1.2000864266836402
1114	23	-1.068571747665871
1114	24	-1.136157365148307
1114	25	-1.722395545997145
1115	1	-0.8211040702120584
1115	2	-1.0195068499111741
1115	3	-0.5690234206381071
1115	4	-0.3073124111605523
1115	5	-0.5840100420215677
1115	6	-0.34735263298441765
1115	7	-0.30217841811891333
1115	8	-0.677418893519949
1115	9	-1.6268173437159206
1115	10	-1.1585485650525627
1115	11	-1.439942720654237
1115	12	-2.057155216473646
1115	13	-2.1166607629531526
1115	14	-1.5102421170120621
1115	15	-1.6701314361263968
1115	16	-1.426455000727941
1115	17	-1.531795060472703
1115	18	-1.7922460905512878
1115	19	-2.1576150179595146
1115	20	-2.3925165162253563
1115	21	-1.7703327135144917
1115	22	-1.7230969934205191
1115	23	-1.5931980106388721
1115	24	-2.0763863459051493
1115	25	-2.079110473258339
1116	1	-1.5551792560517121
1116	2	-1.71934714870779
1116	3	-1.8528269415693508
1116	4	-1.4507988865197916
1116	5	-1.9184021005705745
1116	6	-2.722914392700968
1116	7	-2.1254992207834746
1116	8	-2.4416891098955062
1116	9	-3.6544382665902972
1116	10	-2.295044081058485
1116	11	-3.22506930846977
1116	12	-5.119848637313897
1116	13	-4.219418688266789
1116	14	-3.4103620738582308
1116	15	-4.633347949204126
1116	16	-4.049398611453604
1116	17	-3.9417634455792196
1116	18	-3.844359684365223
1116	19	-4.085148321953046
1116	20	-5.865029561910077
1116	21	-4.71828165944228
1116	22	-3.9101730363357134
1116	23	-4.6272111275415995
1116	24	-4.711293738952879
1116	25	-3.978331403749916
1201	1	0.4792353634210933
1201	2	0.6836202826883842
1201	3	0.7297935567656602
1201	4	0.6076790924455509
1201	5	0.17941873953034815
1201	6	0.6903011897362035
1201	7	0.8327554797958427
1201	8	0.7462329283386069
1201	9	0.7563500875402056
1201	10	0.8398107843342544
1201	11	0.5543422056689167
1201	12	1.058667306216627
1201	13	0.9971247827179539
1201	14	0.9837930533215271
1201	15	0.9479792266486058
1201	16	0.9313278414811492
1201	17	0.783973430499266
1201	18	1.102828721334376
1201	19	1.208228493614385
1201	20	1.2080694840251027
1201	21	0.9045848904602138
1201	22	0.8319822929668135
1201	23	1.017104787425552
1201	24	0.7774723958593484
1201	25	0.9094185741840377
1202	1	0.8570889928628631
1202	2	0.887111698647729
1202	3	0.8563073229658862
1202	4	0.7353204993010962
1202	5	0.8937372479702361
1202	6	0.9208572795252294
1202	7	0.9248466571093914
1202	8	0.898149524606012
1202	9	1.0708594169646588
1202	10	1.2433932723925736
1202	11	1.1783182850662968
1202	12	1.2123078501759963
1202	13	1.1139147406013805
1202	14	1.324430100262596
1202	15	1.2226520622600532
1202	16	1.2101374546775219
1202	17	1.2570793887529916
1202	18	1.1868625799321322
1202	19	1.343163115393601
1202	20	1.2533460497666198
1202	21	1.3189053460530005
1202	22	1.2485675948318828
1202	23	1.4077268207929237
1202	24	1.3015622641395836
1202	25	1.4372762174936398
1203	1	0.7545924965975743
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2211	11	0.018601283384803935
2211	12	-0.18675384709668919
2211	13	0.051084983209314316
2211	14	-0.11685495410038982
2211	15	-0.10691420267217211
2211	16	-0.0767530702513568
2211	17	-0.2364010376504453
2211	18	-0.33803368810806234
2211	19	-0.1445826337211571
2211	20	-0.09508714605435387
2211	21	-0.03347125807342621
2211	22	0.03284622604816434
2211	23	-0.06341763094182795
2211	24	0.0908253422028622
2211	25	-0.20756693606419674
2212	1	-0.10516954173390047
2212	2	0.001288051413460778
2212	3	0.05874944357540457
2212	4	-0.0010106334342836476
2212	5	-0.38718874375478407
2212	6	-0.08664464591618071
2212	7	-0.2790601731200013
2212	8	-6.27353869020908E-4
2212	9	-0.08713324247399612
2212	10	-0.28703792833036346
2212	11	-0.02322455461461459
2212	12	0.20906695766110772
2212	13	0.13786075364530603
2212	14	0.21375455006403143
2212	15	0.015959574720618264
2212	16	0.08355622068060597
2212	17	0.01580165470797823
2212	18	0.017718784630275763
2212	19	0.08699205112998953
2212	20	-0.05371025949754937
2212	21	0.01414680654857392
2212	22	0.1266078782725586
2212	23	-0.271989201955094
2212	24	-0.24662710114201047
2212	25	-0.16112551890443427
2213	1	-0.38961339780819415
2213	2	-0.03435687679047916
2213	3	-0.13667608217673788
2213	4	-0.2737771563649787
2213	5	-0.08791815426621241
2213	6	-0.031104706793051662
2213	7	-0.5151617750549349
2213	8	-0.5279733470099757
2213	9	-0.2660024987209937
2213	10	-0.22317767064756566
2213	11	-0.15790598133706624
2213	12	-0.3931179691509641
2213	13	-0.1662156795252443
2213	14	-0.282723782427567
2213	15	-0.5735892607147122
2213	16	-1.0994719312759642
2213	17	-0.7058919415568283
2213	18	-0.28350217415128753
2213	19	-0.40069630345659846
2213	20	-0.3122886379831087
2213	21	-0.2889203255784949
2213	22	-0.317962016019095
2213	23	-0.5580232126009648
2213	24	-0.2984243451591979
2213	25	-0.3058803221237767
2214	1	-1.0531366255128205
2214	2	-0.37064830762457035
2214	3	-0.5688861984760187
2214	4	-0.6093540968612103
2214	5	-0.9171543662848975
2214	6	-1.4827330738710742
2214	7	-0.9236149383120988
2214	8	-0.7185939542331994
2214	9	-0.5018372649170146
2214	10	-0.9344982912687243
2214	11	-0.954064018079265
2214	12	-0.48222241951597766
2214	13	-0.8707947284547899
2214	14	-0.6654355848165423
2214	15	-0.7497104322240489
2214	16	-0.7029228260412381
2214	17	-0.8605618794704242
2214	18	-0.8123104285552358
2214	19	-0.7312031297359027
2214	20	-1.008403096587962
2214	21	-1.1577684473090173
2214	22	-1.5597404499574026
2214	23	-1.5611570958270846
2214	24	-1.3460543136192982
2214	25	-1.7849858096843008
2215	1	-1.5958340354511726
2215	2	-1.038190207494818
2215	3	-1.0370851728822394
2215	4	-1.1860966216655555
2215	5	-1.6438921632025796
2215	6	-1.4357735118367714
2215	7	-1.9239854917609378
2215	8	-1.526336231192598
2215	9	-1.6683783030060653
2215	10	-1.7134837963753284
2215	11	-1.4492151618242772
2215	12	-1.759210236660877
2215	13	-1.8708213043617832
2215	14	-2.0140893051449495
2215	15	-1.8086431949825013
2215	16	-1.8199669919833
2215	17	-1.9684424758232808
2215	18	-1.7084900402149614
2215	19	-1.9846490241749564
2215	20	-1.9675620301578363
2215	21	-1.5317485476635824
2215	22	-1.7685375738799394
2215	23	-2.289474485912905
2215	24	-2.400191995322217
2215	25	-2.482624825793039
2216	1	-1.8899345196555686
2216	2	-1.2621239437977714
2216	3	-1.2834491950500109
2216	4	-1.4286148454228211
2216	5	-2.3993509977352616
2216	6	-2.647096916946623
2216	7	-2.494233607124009
2216	8	-2.62327901052862
2216	9	-4.727672062629271
2216	10	-2.762014495061436
2216	11	-2.8088440173997213
2216	12	-3.1682068839764845
2216	13	-3.0394179355697517
2216	14	-3.0892963244667513
2216	15	-2.9482966936539867
2216	16	-2.467329435695291
2216	17	-2.7507626565525882
2216	18	-3.1856905324930764
2216	19	-3.454944460704258
2216	20	-2.697571656926982
2216	21	-3.2216894263910305
2216	22	-2.598143494644816
2216	23	-3.6562687863826113
2216	24	-3.0142658262927142
2216	25	-3.1698898992231115
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70542.0
3	1.0
4	1093.0
5	10150.0
6	12121.0
7	9335.0
8	7637.0
9	6518.0
10	5587.0
11	5127.0
12	4559.0
13	4256.0
14	4160.0
15	4239.0
16	4315.0
17	4488.0
18	4829.0
19	5939.0
20	7908.0
21	10383.0
22	12941.0
23	15627.0
24	18256.0
25	20923.0
26	23193.0
27	25584.0
28	29101.0
29	34777.0
30	43681.0
31	55156.0
32	70322.0
33	90715.0
34	118688.0
35	155481.0
36	213091.0
37	331381.0
38	1065086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06034761844124	19.076540964583096	13.411504437142904	26.451606979832764
2	13.795683320039313	20.04976579511352	40.13029604503182	26.02425483981535
3	17.634412720144923	24.842013467102777	29.298189133845643	28.225384678906657
4	11.769558158922381	16.350393496635434	36.70862900427202	35.17141934017016
5	13.199807074955528	38.50745589871515	34.378357288724935	13.914379737604389
6	32.64879835896781	36.2053126022652	17.5370948302697	13.60879420849729
7	28.896973383836144	31.579825539366606	21.07039458216427	18.452806494632977
8	26.37043156222813	36.01804627700756	19.738963516350196	17.872558644414113
9	27.239868106352716	14.448378557906935	19.56298759457664	38.7487657411637
10	16.01769035729478	28.67759670997343	32.85696955863492	22.447743374096866
11	35.159574743907775	22.264876005531693	23.443771796409024	19.131777454151504
12	23.7137834993034	24.759141648841187	30.652578056851944	20.87449679500347
13	29.224149725393623	21.586187392876788	25.623692932271208	23.565969949458385
14	21.587240049187187	21.491912951602153	27.207709768474807	29.713137230735846
15	23.832079168740986	29.95805888510193	23.060113736453108	23.14974820970398
16	22.917210983967454	27.73347672063768	26.571233799621297	22.77807849577357
17	21.728128456490253	28.1046437279697	27.305659739160504	22.861568076379534
18	21.769533454724584	26.974306404551974	29.147781551587627	22.108378589135818
19	23.573798098304398	26.232653977793742	27.713350439207208	22.480197484694656
20	24.44989374397723	26.043694199633688	27.700713797966774	21.805698258422304
21	24.08489707747	25.829010077868453	26.775652456926657	23.31044038773489
22	23.865244026230382	26.843782768532954	27.265294042802857	22.025679162433807
23	22.866156932661667	26.826726335060368	27.586580993063947	22.72053573921402
24	23.46212293444055	25.923603715713604	28.122399977664237	22.491873372181605
25	22.949305337017144	26.471100879192498	27.994720776711112	22.58487300707925
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	88.0
1	88.0
2	340.5
3	593.0
4	593.0
5	593.0
6	1560.5
7	2528.0
8	2528.0
9	2528.0
10	3200.5
11	3873.0
12	3873.0
13	3873.0
14	5851.0
15	7829.0
16	7829.0
17	7829.0
18	15473.0
19	23117.0
20	23117.0
21	23117.0
22	38889.0
23	54661.0
24	54661.0
25	54661.0
26	80324.5
27	105988.0
28	105988.0
29	105988.0
30	136425.0
31	166862.0
32	166862.0
33	166862.0
34	191560.0
35	216258.0
36	216258.0
37	216258.0
38	237678.0
39	259098.0
40	259098.0
41	259098.0
42	277830.5
43	296563.0
44	296563.0
45	296563.0
46	313940.5
47	331318.0
48	331318.0
49	331318.0
50	325534.5
51	319751.0
52	319751.0
53	319751.0
54	291369.5
55	262988.0
56	262988.0
57	262988.0
58	230717.0
59	198446.0
60	198446.0
61	198446.0
62	164602.0
63	130758.0
64	130758.0
65	130758.0
66	101063.0
67	71368.0
68	71368.0
69	71368.0
70	52885.0
71	34402.0
72	34402.0
73	34402.0
74	24090.0
75	13778.0
76	13778.0
77	13778.0
78	9375.0
79	4972.0
80	4972.0
81	4972.0
82	3209.5
83	1447.0
84	1447.0
85	1447.0
86	928.0
87	409.0
88	409.0
89	409.0
90	244.5
91	80.0
92	80.0
93	80.0
94	46.5
95	13.0
96	13.0
97	13.0
98	7.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08324060003430135
2	0.041919439691447395
3	0.019503906764146317
4	0.0071793521831213436
5	0.020620694881520747
6	0.05783367036403304
7	0.07909252988405346
8	0.05623825876778385
9	0.02492830619139355
10	0.04754326556822578
11	0.007299008052840032
12	4.387381889685265E-4
13	0.005863137616215763
14	0.0013162145669055796
15	0.013640769147930552
16	0.009053960808714139
17	0.005224972977716088
18	0.005783367036403304
19	0.002353232104467551
20	0.001515641016436728
21	5.185087687809859E-4
22	8.375910880308232E-4
23	0.0
24	0.0
25	1.1965586971868905E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2507190.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.17499405480859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.40589803566519	31.12466941055875
2	15.720745974359884	14.518107038064477
3	6.557497882108737	9.083772771623726
4	3.301019926824642	6.096983019837302
5	1.9060623017926954	4.400620772668624
6	1.243444453187739	3.4449624140057145
7	0.8257396171838257	2.6689965339998154
8	0.6034085239982397	2.228990800659164
9	0.43064397393139	1.789648464241897
>10	1.876732416217182	14.371316199352254
>50	0.07977801684175836	2.5512450179141237
>100	0.04209559634267339	3.6520145029684863
>500	0.004853297082332688	1.5837535264663392
>1k	0.0018199864058728351	1.6248520425273545
>5k	2.599980579818336E-4	0.8600674851118859
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	9444	0.3766766778744331	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6491	0.2588954167813369	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5557	0.22164255600891836	No Hit
GTCCTACAGTGGACATTTCTAAATT	4113	0.1640481973843227	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3645	0.14538188170820718	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3414	0.13616837973986815	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3383	0.134931935752775	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2814	0.11223720579613032	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.988528990622968E-5
5	0.0	0.0	0.0	0.0	3.988528990622968E-5
6	0.0	0.0	0.0	0.0	3.988528990622968E-5
7	0.0	0.0	0.0	0.0	3.988528990622968E-5
8	0.0	0.0	0.0	0.0	3.988528990622968E-5
9	0.0	0.0	0.0	0.0	3.988528990622968E-5
10	0.0	0.0	0.0	0.0	3.988528990622968E-5
11	0.0	0.0	0.0	0.0	3.988528990622968E-5
12	0.0	0.0	0.0	0.0	3.988528990622968E-5
13	0.0	0.0	0.0	0.0	3.988528990622968E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2000	0.0	14.176134	1
TAGGACC	780	0.0	12.924881	4
TAGCACG	55	0.00304343	12.104607	4
GTCCTAC	1955	0.0	11.436487	1
GTCCTAA	1035	0.0	11.122863	1
TGTAGGA	2230	0.0	11.048119	2
AGGACCT	1395	0.0	10.976578	5
GTAGGAC	2030	0.0	10.965116	3
TAGGACT	245	0.0	10.869442	4
CTTACAC	325	0.0	10.829585	3
TCCTACA	2295	0.0	10.776658	2
CGTCTGA	90	9.607148E-5	10.546016	19
GTATCAA	4455	0.0	10.507247	1
GTTTAGG	255	0.0	10.446947	1
CTGTAGG	2200	0.0	10.422356	1
CTAGTAC	130	3.7753307E-7	10.244202	3
ATTTCTA	1420	0.0	10.227481	15
TCGAAAC	215	1.8189894E-12	10.154213	16
GGACCTG	1385	0.0	10.094455	6
CCGATCA	85	6.6084403E-4	10.056753	9
>>END_MODULE