FastQCFastQC Report
Thu 26 May 2016
SRR1512894_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512894_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2403733
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT70630.29383463138376853No Hit
GTCCTAAAGTGTGTATTTCTCATTT63410.26379801750027976No Hit
CTGTAGGACGTGGAATATGGCAAGA60500.25169184763865204No Hit
GTATCAACGCAGAGTACTTTTTTTT52910.22011596129853025No Hit
CTTTAGGACGTGAAATATGGCGAGG50710.21096353047530653No Hit
TATCAACGCAGAGTACTTTTTTTTT31240.12996451768977668No Hit
GTCCTACAGTGTGCATTTCTCATTT30200.1256379140278891No Hit
GGTATCAACGCAGAGTACTTTTTTT29580.12305859261407152No Hit
CTGAAGGACCTGGAATATGGCGAGA27400.11398936570742259No Hit
GTCCTTCAGTGTGCATTTCTCATTT25220.10492013880077362No Hit
GTACTGGTTCACTATCGGTCAGTCA25140.10458732313447458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAC400.005286433714.2474714
GGTATCA18200.012.3527521
TAGGACC8350.012.1714934
GTCCTAA17900.011.28251651
CCGACGA600.005885921411.0811369
GAAATGT9150.011.0039696
GTCCTAC32950.010.9573841
TTATACG700.00149412610.8552164
TTTAGAA9300.010.82603552
AATGTCC8950.010.8253458
CCCCGTA1502.1409505E-910.76497915
CCCGTAC1605.456968E-1010.68649316
ATTTAGA8750.010.5605571
GTCCTAG3250.010.5521821
TAGAAAT9200.010.5307394
CTAGAGT2800.010.5159914
TCTAGGA4350.010.4808992
AGGACCT17350.010.4563575
GGCGAGG15050.010.413650519
TCCTACA35950.010.3834142
Produced by FastQC (version 0.11.5)