##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512894_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2403733
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.437528627347547	31.0	29.0	33.0	16.0	34.0
2	28.62815712061198	31.0	29.0	34.0	16.0	34.0
3	28.7275279741968	31.0	29.0	34.0	16.0	34.0
4	32.1475825310049	35.0	33.0	37.0	19.0	37.0
5	32.31879955053244	35.0	33.0	37.0	22.0	37.0
6	32.2114348806627	35.0	33.0	37.0	21.0	37.0
7	32.14041700970948	35.0	33.0	37.0	19.0	37.0
8	32.050407428778485	35.0	33.0	37.0	18.0	37.0
9	33.318039482754536	38.0	33.0	39.0	17.0	39.0
10	33.18260014735414	38.0	33.0	39.0	17.0	39.0
11	33.229436880052816	38.0	33.0	39.0	17.0	39.0
12	33.12466151606689	38.0	33.0	39.0	17.0	39.0
13	33.08790784999832	38.0	33.0	39.0	17.0	39.0
14	33.908095449869016	39.0	33.0	41.0	16.0	41.0
15	34.063196286775614	39.0	33.0	41.0	17.0	41.0
16	33.99885386604918	39.0	33.0	41.0	16.0	41.0
17	33.763975449852374	39.0	33.0	41.0	10.0	41.0
18	33.74381888504256	39.0	33.0	41.0	10.0	41.0
19	33.847451443234334	39.0	33.0	41.0	10.0	41.0
20	33.81811623836757	39.0	33.0	41.0	10.0	41.0
21	33.71935651755	39.0	33.0	41.0	8.0	41.0
22	33.70149097258306	39.0	33.0	41.0	2.0	41.0
23	33.3561510367416	39.0	33.0	41.0	2.0	41.0
24	33.41001517223419	39.0	33.0	41.0	2.0	41.0
25	33.105338654501146	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5419416712592309
1101	2	0.22871946676805877
1101	3	0.5799535285692521
1101	4	0.647514243160991
1101	5	0.46763878968678796
1101	6	0.3562010891380538
1101	7	0.02632418044444762
1101	8	0.45244598166118877
1101	9	0.46642200179424975
1101	10	0.5073180675797104
1101	11	0.5619729296909526
1101	12	0.41744401687461163
1101	13	0.895867386524948
1101	14	0.9832267440810085
1101	15	0.7714662207701579
1101	16	0.5832212673210151
1101	17	-0.03927866836458804
1101	18	0.3844582371054912
1101	19	0.02514599106715565
1101	20	-0.8030839426465732
1101	21	0.5373065523001301
1101	22	0.49570881531229816
1101	23	0.14708757377811565
1101	24	0.6260366206542685
1101	25	0.13749748152778807
1102	1	1.0477168769970753
1102	2	1.1690351197942022
1102	3	1.0711122205177155
1102	4	1.1001807539080346
1102	5	1.059904361547808
1102	6	0.96520506698986
1102	7	0.9808617945591394
1102	8	0.9790285114923627
1102	9	1.2488513816144433
1102	10	1.231535576538299
1102	11	1.0120032036314015
1102	12	1.2148314939756162
1102	13	1.0811608794069727
1102	14	1.2935643100616332
1102	15	1.219353754371646
1102	16	1.2968111835047083
1102	17	1.359620224008495
1102	18	1.3567641687877057
1102	19	1.233004638793119
1102	20	1.473114152960946
1102	21	1.4959759005669753
1102	22	1.3849951543630468
1102	23	1.2845731634367823
1102	24	0.6818746560235738
1102	25	1.0726504022771621
1103	1	0.6160311521843447
1103	2	0.7260367122503233
1103	3	0.876131579435615
1103	4	0.8878443353748651
1103	5	0.80950500519954
1103	6	0.3557669774402754
1103	7	0.3547775529438937
1103	8	0.47822031794437336
1103	9	0.6425226190322917
1103	10	0.7507204063913449
1103	11	0.5957309240391311
1103	12	0.6638307574115387
1103	13	0.9122418566669879
1103	14	0.8169968229090045
1103	15	0.8744520168585055
1103	16	0.8096091233483804
1103	17	0.49957014549723766
1103	18	0.8415425310000444
1103	19	0.7923663747958685
1103	20	0.895801961272511
1103	21	0.6810449326080033
1103	22	0.6965184754595413
1103	23	0.02453746966567394
1103	24	0.32445773688836965
1103	25	0.6071914653159993
1104	1	0.8852771580694956
1104	2	0.7881623653088887
1104	3	0.7998723810772432
1104	4	1.045552400780565
1104	5	0.825713634657788
1104	6	0.8776194711175549
1104	7	0.8372477499738338
1104	8	0.6540122226827343
1104	9	0.9880327029563745
1104	10	0.9903791754662379
1104	11	0.7070337409631477
1104	12	0.9925602619506009
1104	13	1.0290800291238895
1104	14	1.3347292610349726
1104	15	0.906665805936882
1104	16	0.8986863436511925
1104	17	1.1803951839086722
1104	18	0.9471284169092584
1104	19	0.5148319746530774
1104	20	0.6896403372634623
1104	21	0.795601083926897
1104	22	0.4810069340519192
1104	23	0.9132571891610866
1104	24	0.8462651577267906
1104	25	0.3810984008188214
1105	1	0.5583559438300156
1105	2	0.7347495781062356
1105	3	0.8113986671695983
1105	4	0.9192521638590421
1105	5	0.6784658551244647
1105	6	0.7704328514696215
1105	7	0.8160064256937076
1105	8	0.7860285822089743
1105	9	0.4210949405964044
1105	10	0.6598290593629059
1105	11	0.657658946277202
1105	12	0.9120376918824533
1105	13	0.8130998950682269
1105	14	0.9809210665123018
1105	15	1.0095840713412656
1105	16	0.8665617730490354
1105	17	1.2702295950820641
1105	18	1.078139922695975
1105	19	0.8791974754348715
1105	20	0.9474251030504135
1105	21	0.8207567638681041
1105	22	0.8124910529298504
1105	23	1.0694415431155946
1105	24	1.1505855547586847
1105	25	1.402321019419233
1106	1	0.7723852751369655
1106	2	0.8655080917579561
1106	3	0.6295017823651818
1106	4	0.9000596758128694
1106	5	0.7895596687740607
1106	6	0.9391238020235733
1106	7	0.8695332017706789
1106	8	0.8419261888727441
1106	9	1.051379340591474
1106	10	0.7368185596143206
1106	11	0.5866869516397344
1106	12	0.7665842123341307
1106	13	0.8316556412601699
1106	14	1.1555597499373675
1106	15	0.7049792932723591
1106	16	0.9621634147544569
1106	17	1.108166792793618
1106	18	1.1203403661463511
1106	19	1.1353916424860273
1106	20	1.2200521281321386
1106	21	1.2254139501851995
1106	22	1.1533462464219895
1106	23	1.4386271588088988
1106	24	1.185843741449034
1106	25	1.3101489309820167
1107	1	0.9959931308345737
1107	2	1.0270970055019752
1107	3	0.9163028648589346
1107	4	0.9824117131325067
1107	5	0.9456785577657598
1107	6	0.9638135674793631
1107	7	0.7234987442290262
1107	8	0.8804156343250504
1107	9	0.8407612282486809
1107	10	1.1587761483157522
1107	11	0.9991429362357565
1107	12	1.0373678601013694
1107	13	0.8584909406894994
1107	14	1.2140285193592675
1107	15	0.9299753058819817
1107	16	0.9882515894848254
1107	17	1.2129333497427055
1107	18	1.1484842044569845
1107	19	1.0998529956862768
1107	20	1.2436785029707096
1107	21	1.0885920190579768
1107	22	1.1278847635747766
1107	23	1.3369851066804372
1107	24	1.1605453879654846
1107	25	1.4604274772904517
1108	1	0.7048826643647033
1108	2	0.655451481269619
1108	3	0.6304660340702064
1108	4	0.7349913225214166
1108	5	0.5370152435102113
1108	6	0.6844301488417344
1108	7	0.7523979141773438
1108	8	0.6636278484228519
1108	9	0.8107780235046746
1108	10	0.8366131334863738
1108	11	0.5648511071464739
1108	12	0.7315801576151983
1108	13	0.7594036613840203
1108	14	0.8626815995893011
1108	15	0.876737705922821
1108	16	0.4567085051088995
1108	17	0.8569869700603618
1108	18	0.9252766218159891
1108	19	0.8129791916790694
1108	20	0.9562031075849902
1108	21	0.8226069567511871
1108	22	0.8571264372239042
1108	23	1.1381973780489503
1108	24	1.0384335432637073
1108	25	1.2358183809598842
1109	1	0.6544980554591469
1109	2	0.657362640324699
1109	3	0.7786903569258143
1109	4	0.7881036006202606
1109	5	0.5668105950113684
1109	6	0.773354947178639
1109	7	0.6506959519201132
1109	8	0.5783060016762
1109	9	0.626231196925886
1109	10	0.6881646400090347
1109	11	0.6992049897654198
1109	12	0.5398621172145752
1109	13	0.6802359938628015
1109	14	0.7728920629230842
1109	15	0.6997617070907793
1109	16	0.34097800399116096
1109	17	0.6886717061810259
1109	18	0.7056246633958025
1109	19	0.6371481912467019
1109	20	0.6355966302831249
1109	21	0.6302107893014082
1109	22	0.6340561419837698
1109	23	0.9919293852838038
1109	24	0.7118974317037328
1109	25	0.32252043992306056
1110	1	0.6771053334983215
1110	2	0.7055606995390846
1110	3	0.5847311157827981
1110	4	0.6303187607496668
1110	5	0.5321700364286599
1110	6	0.554641097895896
1110	7	0.4729563196524893
1110	8	0.5310572897079773
1110	9	0.630737430198856
1110	10	0.6066068836023959
1110	11	0.5209639268342272
1110	12	0.6315981028266151
1110	13	0.6319301615108728
1110	14	0.5821657675812304
1110	15	0.5275195602200782
1110	16	0.6498082619203416
1110	17	0.6982758075200621
1110	18	0.043676651518403276
1110	19	0.5122143543926398
1110	20	0.5690735369705848
1110	21	0.813505021762353
1110	22	0.7664839367752734
1110	23	0.8464558881013602
1110	24	0.7550836054531516
1110	25	1.047252574632033
1111	1	0.3152467088219595
1111	2	0.24347360163755383
1111	3	0.4618007462549123
1111	4	0.29808900486779777
1111	5	0.38412853045942086
1111	6	0.13231497992404684
1111	7	-0.02681483134401219
1111	8	0.3189591063456909
1111	9	0.38355758394229866
1111	10	0.3992129439048
1111	11	0.034885637176770956
1111	12	-0.3454449726434561
1111	13	0.19714310882800135
1111	14	0.20441535203302408
1111	15	0.07997796469033602
1111	16	0.2685053632915597
1111	17	-0.13593217075494124
1111	18	0.16405373254748667
1111	19	0.20914211601890287
1111	20	0.35083137789860075
1111	21	0.25936463348313055
1111	22	-0.03815517197872964
1111	23	0.3494855382642612
1111	24	0.39713132178560073
1111	25	0.3795358162963254
1112	1	0.4738308967986953
1112	2	0.38713232940055065
1112	3	0.41513125543889373
1112	4	0.5356811807903128
1112	5	0.37477636833633454
1112	6	0.0867102526024297
1112	7	0.03134877822326132
1112	8	0.2762884080789938
1112	9	0.26702058293524544
1112	10	0.31447438488049784
1112	11	0.3097752290026321
1112	12	0.2786634543983695
1112	13	0.3056542409652181
1112	14	0.3425102941844713
1112	15	0.10400496491102729
1112	16	0.2773647789970397
1112	17	0.638748019697573
1112	18	0.273837380722739
1112	19	0.4425328201706549
1112	20	0.21718256848048867
1112	21	0.45213489569778886
1112	22	0.2768334510962589
1112	23	0.33042785712763134
1112	24	0.3745768533302538
1112	25	0.3782506169528119
1113	1	-0.10772351931425561
1113	2	0.1356660662488025
1113	3	0.0689802864387623
1113	4	0.19209901034025734
1113	5	0.16916775593507793
1113	6	-0.1825713211057547
1113	7	-0.23920456304563587
1113	8	-0.008624141086265524
1113	9	-0.13200063965721398
1113	10	-0.039797693715662774
1113	11	-0.12136427630662183
1113	12	-0.3237618347530571
1113	13	-0.2669218150789092
1113	14	0.21079681689990082
1113	15	-0.13345827187882264
1113	16	-0.09288349816836217
1113	17	0.02150359700956983
1113	18	-0.2872662570797502
1113	19	-0.7484481357974815
1113	20	-0.4812881082250584
1113	21	-0.18196049638405754
1113	22	-0.8195468573406757
1113	23	-4.531481454381598
1113	24	-1.9066181002834668
1113	25	-1.1769583739405398
1114	1	-0.6265415406095869
1114	2	-1.0052290905309675
1114	3	-0.7664806203115404
1114	4	-0.6511095205037662
1114	5	-0.719659027670243
1114	6	-1.4236498999301404
1114	7	-1.3761563914303174
1114	8	-1.0856782281997468
1114	9	-1.268563032042298
1114	10	-0.9800002004354624
1114	11	-1.164920150549996
1114	12	-2.0941733513116
1114	13	-1.2735198373911665
1114	14	-3.13598200136407
1114	15	-1.8704752903475637
1114	16	-1.8347413823406242
1114	17	-1.6703255561462491
1114	18	-1.5518519064209428
1114	19	-1.685825787024612
1114	20	-1.2606929818374724
1114	21	-1.1908577222592527
1114	22	-1.4431228014051882
1114	23	-5.614748244620042
1114	24	-2.8575191910509474
1114	25	-2.792603098527241
1115	1	-1.8158892007286944
1115	2	-1.762934327537934
1115	3	-1.453558117560867
1115	4	-1.423036781731625
1115	5	-1.4758319027909366
1115	6	-1.5556164783174609
1115	7	-2.1247196949574167
1115	8	-2.4204420726301663
1115	9	-3.7885884461402135
1115	10	-4.489023156392257
1115	11	-3.1997436084890225
1115	12	-3.469272447269546
1115	13	-2.7027540961814687
1115	14	-3.4993470755511566
1115	15	-3.1008539788067147
1115	16	-3.175486381230371
1115	17	-2.9557058538426695
1115	18	-5.774151506481822
1115	19	-3.6337208048759173
1115	20	-4.027160375446929
1115	21	-3.618664406706536
1115	22	-3.2537005269416603
1115	23	-3.530388346812849
1115	24	-3.452519156745499
1115	25	-3.5474484308659235
1116	1	-0.9118473841331429
1116	2	-0.8352122703103326
1116	3	-1.2352083791014614
1116	4	-0.8090707494308305
1116	5	-0.8301026444918342
1116	6	-3.426503740473862
1116	7	-1.9278186896952114
1116	8	-2.1587962063319956
1116	9	-3.1546529637642244
1116	10	-2.558099598602979
1116	11	-1.8822822746002217
1116	12	-1.8234649516619825
1116	13	-3.3529979461746358
1116	14	-6.716185637973005
1116	15	-4.350793774699529
1116	16	-2.9711849737436857
1116	17	-4.086856249993794
1116	18	-2.6149079366729
1116	19	-2.3805522250288256
1116	20	-3.1065034710854356
1116	21	-4.101865996967035
1116	22	-3.4570280792687775
1116	23	-2.457986818992694
1116	24	-3.437754356408231
1116	25	-3.300005999758671
1201	1	0.6707031778444197
1201	2	0.6446344846388072
1201	3	0.9895165310105156
1201	4	0.8670757295319049
1201	5	0.4596222206483844
1201	6	0.8158512656241896
1201	7	0.9655351741202693
1201	8	0.9306496937566529
1201	9	1.4688263338680727
1201	10	1.3085800573081983
1201	11	1.4822107611439108
1201	12	1.245307140712569
1201	13	1.411280907206816
1201	14	1.3957596894558293
1201	15	1.4449540933180671
1201	16	1.4366901528926874
1201	17	1.656888616947633
1201	18	1.4249127416670362
1201	19	1.607529559085961
1201	20	1.6546038806984669
1201	21	1.7264338289046677
1201	22	1.624528840546816
1201	23	1.8678435903005024
1201	24	1.6842088090379477
1201	25	1.9097528943987285
1202	1	1.155266834657926
1202	2	1.0312824919781427
1202	3	1.0550760775888612
1202	4	1.1703982300890985
1202	5	0.998614513309569
1202	6	1.4144854028635407
1202	7	1.4249166174192354
1202	8	1.3701373915584831
1202	9	1.6016834397027466
1202	10	1.4287197949373223
1202	11	1.401518914751641
1202	12	1.6536928497410912
1202	13	1.5798333062261634
1202	14	2.073774129701924
1202	15	1.6642486670820134
1202	16	1.460100352288336
1202	17	1.852243273745593
1202	18	1.7203989467226748
1202	19	1.7582108836908716
1202	20	1.5147580274748051
1202	21	1.64327293885475
1202	22	1.6690678255830704
1202	23	1.7960126438061224
1202	24	1.8281269567887222
1202	25	2.1483051379236855
1203	1	0.9953384083077879
1203	2	1.1060145760073787
1203	3	0.9712879178863218
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2211	20	0.1289797765062417
2211	21	0.28631792155371016
2211	22	0.35349570835005295
2211	23	0.3934503157074616
2211	24	0.030085966637003025
2211	25	0.41989890363509375
2212	1	-0.030653396875830907
2212	2	0.10483866280303289
2212	3	-0.33272715309073675
2212	4	0.04759844526149948
2212	5	-0.29135814329623955
2212	6	-0.4577954074358601
2212	7	-0.20549095554947172
2212	8	-0.02349171789930793
2212	9	-0.051742837110026585
2212	10	-0.41436617606252923
2212	11	-0.6779818577722878
2212	12	-0.15127508922694233
2212	13	-0.08194963431441238
2212	14	0.00787108446244389
2212	15	-0.4100849429506539
2212	16	-0.4729621250472391
2212	17	-0.2983057080524034
2212	18	0.026584947111082613
2212	19	-0.21050181130911483
2212	20	-0.07063939698713284
2212	21	0.12854864998064386
2212	22	0.07242857105577372
2212	23	0.09126953593978726
2212	24	0.01899299134348098
2212	25	0.2580571568836021
2213	1	-0.5221210255539681
2213	2	-0.43458600531370806
2213	3	-0.7336210715063238
2213	4	-0.47515942480726636
2213	5	-0.44084644594922295
2213	6	-0.18337410924965525
2213	7	-0.5387557594544248
2213	8	-0.28044251635043693
2213	9	-0.7125835404654808
2213	10	-0.3257061753080208
2213	11	-0.48467910906887823
2213	12	-0.595371334105181
2213	13	-0.5048791540613067
2213	14	-0.6100272866533416
2213	15	-0.5095088030723574
2213	16	-0.5070845016675207
2213	17	-0.5406035023667144
2213	18	-0.43349854946352195
2213	19	-0.5698293116877764
2213	20	-0.2817758208968044
2213	21	-0.5448376544189628
2213	22	-0.737476589470603
2213	23	-0.39203832818866857
2213	24	-0.20545472856208136
2213	25	0.03927307399175817
2214	1	-2.876557716684921
2214	2	-1.5118267517193367
2214	3	-1.7349512719336246
2214	4	-3.560466882360082
2214	5	-1.9136471892717246
2214	6	-1.6641828518399144
2214	7	-1.3722937312871721
2214	8	-1.2659876516346493
2214	9	-1.1302249246928824
2214	10	-1.029275014535024
2214	11	-1.0133988528803428
2214	12	-1.0223966831849367
2214	13	-1.4098586440447605
2214	14	-1.419301294693554
2214	15	-1.793924502448263
2214	16	-1.702588135180143
2214	17	-3.9090265675242506
2214	18	-3.7251038948338966
2214	19	-2.5507530693444096
2214	20	-2.2043423790920187
2214	21	-2.181792898503815
2214	22	-3.374828966729652
2214	23	-1.949962565175106
2214	24	-3.3462864621221513
2214	25	-3.1826325389843504
2215	1	-2.2137237709862916
2215	2	-3.7257905285647652
2215	3	-2.2760039787430664
2215	4	-3.971912253084497
2215	5	-2.675467289616119
2215	6	-2.471654776965295
2215	7	-2.6570025451280124
2215	8	-2.1296246991845713
2215	9	-4.632638515113882
2215	10	-2.88837549990253
2215	11	-2.329055446620316
2215	12	-2.304422957597641
2215	13	-2.3966868480214423
2215	14	-2.076175677108459
2215	15	-2.3326930643454595
2215	16	-2.3727861647300266
2215	17	-2.696880179454631
2215	18	-2.2495686871274145
2215	19	-2.432324820752772
2215	20	-2.3148281685428067
2215	21	-3.037443411657538
2215	22	-2.843757874242268
2215	23	-2.8580792319671566
2215	24	-2.761376831932349
2215	25	-3.7775803699093835
2216	1	-3.444425823912944
2216	2	-3.3782308198017503
2216	3	-4.780453825999281
2216	4	-5.574788819524798
2216	5	-5.283558092607258
2216	6	-3.9885910973787517
2216	7	-5.775850627139963
2216	8	-3.48714865921988
2216	9	-4.299290129609403
2216	10	-2.9500925479021873
2216	11	-3.346785331082927
2216	12	-3.9428747048597472
2216	13	-3.346480742324527
2216	14	-4.537724798653382
2216	15	-3.85182156723328
2216	16	-4.181735499229205
2216	17	-3.9811138310537864
2216	18	-3.345630856813152
2216	19	-4.058730504938843
2216	20	-4.861600502165995
2216	21	-4.466265108750864
2216	22	-4.049037882702947
2216	23	-4.101069541336468
2216	24	-4.703646428330728
2216	25	-4.741821988790768
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	138569.0
3	4.0
4	1281.0
5	7263.0
6	8531.0
7	7785.0
8	6619.0
9	6307.0
10	5808.0
11	5425.0
12	5212.0
13	5060.0
14	5244.0
15	5183.0
16	5391.0
17	5793.0
18	6654.0
19	7819.0
20	10063.0
21	13021.0
22	16281.0
23	19573.0
24	22576.0
25	25473.0
26	28378.0
27	31136.0
28	35308.0
29	41340.0
30	49729.0
31	61706.0
32	76347.0
33	95626.0
34	122141.0
35	157630.0
36	210356.0
37	317755.0
38	835346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.40273117329742	18.291563937358013	12.562462063558725	24.743242825785845
2	14.9457515543094	19.616231304704556	41.931425238165176	23.50659190282087
3	19.26113676579559	25.093415735283042	28.59060296575457	27.0548445331668
4	12.432539935151796	15.974518392034149	37.62369152126165	33.96925015155241
5	13.293293334942144	38.52864447893219	34.60616701963444	13.571895166491224
6	32.19560410370255	35.727617804916335	18.43672598113689	13.640052110244227
7	29.177470793468395	31.305113537008815	21.393170293022905	18.12424537649988
8	26.060538923352677	36.32710208759504	20.235778966797042	17.376580022255244
9	28.114597346663718	14.402403932830113	19.643165700072156	37.839833020434014
10	16.82479285365406	27.978737180744375	33.05155850585639	22.144911459745174
11	34.87045799378105	21.77394103823471	24.58308559132478	18.772515376659456
12	24.961407912637593	24.079671891260055	30.58233971114338	20.376580484958975
13	29.783415898267346	21.709164915857045	25.329364553953663	23.178054631921942
14	21.592899203296405	22.163216394089723	26.809904540469326	29.433979862144543
15	23.50305054625867	31.200095265813687	22.669099942082717	22.627754245844926
16	22.45913243340023	27.235786914051115	27.812929390504905	22.492151262043752
17	22.031041847902358	28.363925293346753	27.23942766931283	22.36560518943806
18	21.920937222937344	26.369722821779973	29.93990356085249	21.7694363944302
19	24.330697710055897	25.94017962436122	27.088358062775246	22.640764602807632
20	25.01473041978469	26.148566745770236	27.554067407233752	21.28263542721132
21	24.08803829504287	25.920726063620503	26.281667751282072	23.709567890054554
22	24.530573473075414	27.744483048839346	26.186772810012656	21.538170668072585
23	22.822470933174095	27.385632590989857	27.445346538693933	22.346549937142118
24	24.109699584897758	25.966937558112857	27.918904952870548	22.004457904118834
25	23.20836478817584	26.804730697275264	27.732282474409676	22.254622040139225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	268.0
1	268.0
2	632.0
3	996.0
4	996.0
5	996.0
6	1591.5
7	2187.0
8	2187.0
9	2187.0
10	2600.0
11	3013.0
12	3013.0
13	3013.0
14	4598.5
15	6184.0
16	6184.0
17	6184.0
18	12746.0
19	19308.0
20	19308.0
21	19308.0
22	33553.0
23	47798.0
24	47798.0
25	47798.0
26	74628.5
27	101459.0
28	101459.0
29	101459.0
30	134654.0
31	167849.0
32	167849.0
33	167849.0
34	192295.5
35	216742.0
36	216742.0
37	216742.0
38	235502.0
39	254262.0
40	254262.0
41	254262.0
42	271108.5
43	287955.0
44	287955.0
45	287955.0
46	305149.0
47	322343.0
48	322343.0
49	322343.0
50	314327.0
51	306311.0
52	306311.0
53	306311.0
54	273800.0
55	241289.0
56	241289.0
57	241289.0
58	210837.5
59	180386.0
60	180386.0
61	180386.0
62	151084.5
63	121783.0
64	121783.0
65	121783.0
66	94639.5
67	67496.0
68	67496.0
69	67496.0
70	50470.5
71	33445.0
72	33445.0
73	33445.0
74	24066.0
75	14687.0
76	14687.0
77	14687.0
78	10088.0
79	5489.0
80	5489.0
81	5489.0
82	3651.0
83	1813.0
84	1813.0
85	1813.0
86	1177.5
87	542.0
88	542.0
89	542.0
90	318.5
91	95.0
92	95.0
93	95.0
94	60.0
95	25.0
96	25.0
97	25.0
98	16.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3444226126612232
2	0.010566897404994648
3	0.009568450406097516
4	0.011398936570742257
5	0.012605393361076293
6	0.07912692466259771
7	0.07792046787226369
8	0.10404649767673864
9	0.08328712049133577
10	0.09027624948361569
11	0.08490959686454361
12	0.09709897064274609
13	0.08266309111702506
14	0.08682328694576312
15	0.08453517923995718
16	0.08624085952973978
17	0.09959508813998892
18	0.10092635080518511
19	0.09472765902036541
20	0.09318838656373234
21	0.08153983824326579
22	0.102008001720657
23	0.09501887272837707
24	0.08969382206759235
25	0.08911139465156905
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2403733.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.656791652128064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.21236116645412	37.087033259694174
2	13.823893371405163	14.005481726730112
3	5.2846141068610875	8.031047873194769
4	2.546286844975486	5.1594688876991075
5	1.4564858515301542	3.689045016261768
6	0.8719909670087629	2.650335884298035
7	0.584352013110894	2.0720978725762116
8	0.39769459031558	1.6116745602235811
9	0.30303133389913794	1.3815535630855575
>10	1.3914565393107794	12.090488446426114
>50	0.07520720274671516	2.624167302941482
>100	0.04477215670371335	4.383284928932241
>500	0.004546969076949606	1.633869896385503
>1k	0.0028935257762397527	2.331784537879417
>5k	4.133608251771075E-4	1.2486662436719893
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	7063	0.29383463138376853	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	6341	0.26379801750027976	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6050	0.25169184763865204	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5291	0.22011596129853025	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5071	0.21096353047530653	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3124	0.12996451768977668	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3020	0.1256379140278891	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2958	0.12305859261407152	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2740	0.11398936570742259	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2522	0.10492013880077362	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2514	0.10458732313447458	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	4.16019582873805E-5
5	0.0	0.0	0.0	0.0	4.16019582873805E-5
6	0.0	0.0	0.0	0.0	4.16019582873805E-5
7	0.0	0.0	0.0	0.0	4.16019582873805E-5
8	0.0	0.0	0.0	0.0	4.16019582873805E-5
9	0.0	0.0	0.0	0.0	4.16019582873805E-5
10	0.0	0.0	0.0	0.0	4.16019582873805E-5
11	0.0	0.0	0.0	0.0	4.16019582873805E-5
12	0.0	0.0	0.0	0.0	4.16019582873805E-5
13	0.0	0.0	0.0	0.0	4.16019582873805E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGAC	40	0.0052864337	14.247471	4
GGTATCA	1820	0.0	12.352752	1
TAGGACC	835	0.0	12.171493	4
GTCCTAA	1790	0.0	11.2825165	1
CCGACGA	60	0.0058859214	11.081136	9
GAAATGT	915	0.0	11.003969	6
GTCCTAC	3295	0.0	10.957384	1
TTATACG	70	0.001494126	10.855216	4
TTTAGAA	930	0.0	10.8260355	2
AATGTCC	895	0.0	10.825345	8
CCCCGTA	150	2.1409505E-9	10.764979	15
CCCGTAC	160	5.456968E-10	10.686493	16
ATTTAGA	875	0.0	10.560557	1
GTCCTAG	325	0.0	10.552182	1
TAGAAAT	920	0.0	10.530739	4
CTAGAGT	280	0.0	10.515991	4
TCTAGGA	435	0.0	10.480899	2
AGGACCT	1735	0.0	10.456357	5
GGCGAGG	1505	0.0	10.4136505	19
TCCTACA	3595	0.0	10.383414	2
>>END_MODULE