FastQCFastQC Report
Thu 26 May 2016
SRR1512894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512894_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2403733
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66860.27815069310942603No Hit
GTCCTACAGTGGACATTTCTAAATT65650.27311685615665304No Hit
CTGTAGGACGTGGAATATGGCAAGA60800.2529399063872735No Hit
GTCCTAAAGTGTGTATTTCTCATTT58960.24528514606239546No Hit
CTTTAGGACGTGAAATATGGCGAGG55760.23197251941043368No Hit
TATCAACGCAGAGTACTTTTTTTTT45790.19049536699791533No Hit
GGTATCAACGCAGAGTACTTTTTTT38120.15858666499149449No Hit
GTCCTACAGTGTGCATTTCTCATTT29390.1222681554066113No Hit
CTGAAGGACCTGGAATATGGCGAGA27740.11540383228919353No Hit
GTACTGGTTCACTATCGGTCAGTCA25240.1050033427173484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17450.013.3002971
CCCGTAC1403.6379788E-1212.88267916
TAGGACC8600.012.8278544
GCCCCGT1302.6466296E-911.68405114
GGCGAGG14600.011.50755319
CCCCGTA1657.2759576E-1211.50701915
AGGACCT18450.011.3917485
CCGTACA1604.5474735E-1111.27234517
CACCTTT8750.010.95797114
TATGTCG1658.185452E-1110.93075816
TCCAACG1158.9124114E-710.73065318
GTATCAA38600.010.719361
CGAATTT803.7969957E-410.67995215
TCGAATT803.7969957E-410.67995214
GGACCTG19450.010.5613546
AATCCCG909.599677E-510.54678419
CCACCTT9400.010.50321613
TAGAAAT8550.010.4557754
CGCCCTA3100.010.4111316
TCCCGCC3300.010.35631713
Produced by FastQC (version 0.11.5)