##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512894_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2403733
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.525939445021557	31.0	31.0	34.0	27.0	34.0
2	30.5173902425935	31.0	31.0	34.0	27.0	34.0
3	30.708753010421706	31.0	31.0	34.0	27.0	34.0
4	34.33741684288563	37.0	35.0	37.0	31.0	37.0
5	33.97579722872715	37.0	35.0	37.0	30.0	37.0
6	33.881463124232184	37.0	35.0	37.0	29.0	37.0
7	33.72871654214507	37.0	35.0	37.0	28.0	37.0
8	33.68020949082115	37.0	35.0	37.0	28.0	37.0
9	35.05632613938403	39.0	35.0	39.0	28.0	39.0
10	34.95978172284526	39.0	35.0	39.0	28.0	39.0
11	34.971331674524585	39.0	35.0	39.0	27.0	39.0
12	34.857231231588536	39.0	35.0	39.0	27.0	39.0
13	34.86009053418162	39.0	35.0	39.0	27.0	39.0
14	35.95944017076772	40.0	36.0	41.0	27.0	41.0
15	35.967729776976064	40.0	36.0	41.0	27.0	41.0
16	35.96479808697555	40.0	36.0	41.0	27.0	41.0
17	35.946663377338496	40.0	36.0	41.0	27.0	41.0
18	35.86925086937692	40.0	36.0	41.0	27.0	41.0
19	35.87266722219148	40.0	36.0	41.0	27.0	41.0
20	35.8587259899498	40.0	36.0	41.0	27.0	41.0
21	35.82437317289399	40.0	36.0	41.0	27.0	41.0
22	35.7854412282895	40.0	36.0	41.0	27.0	41.0
23	35.634986082064856	40.0	36.0	41.0	27.0	41.0
24	35.60778380959948	40.0	36.0	41.0	27.0	41.0
25	35.543527088907126	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6110962432634466
1101	2	0.476195737665833
1101	3	0.42881890044681725
1101	4	0.5075620999909987
1101	5	0.5713587815591339
1101	6	0.7143049249723035
1101	7	0.7358755957297873
1101	8	0.5146396560429451
1101	9	0.7493320103017425
1101	10	0.4447185889449301
1101	11	0.8155785262180331
1101	12	0.8983633455434372
1101	13	1.0752370042645438
1101	14	1.0374447083710905
1101	15	0.6895573686079999
1101	16	0.8533522732930194
1101	17	1.0117861581893166
1101	18	0.914018060567642
1101	19	0.651189271436678
1101	20	0.7565805868134277
1101	21	0.8610161431748793
1101	22	0.9231189564815239
1101	23	1.093574227640353
1101	24	1.0408047041364767
1101	25	1.05456646535972
1102	1	0.741937864240775
1102	2	0.8732601297531559
1102	3	0.5602399909931997
1102	4	0.6399205418161387
1102	5	0.8288696117251817
1102	6	0.7570992925253748
1102	7	0.8078483239211423
1102	8	0.9011112248877069
1102	9	1.1201772549162712
1102	10	1.0907947657306138
1102	11	1.1902965670418126
1102	12	1.073071960564313
1102	13	1.190199406991347
1102	14	1.219962071406293
1102	15	1.3577471716820568
1102	16	1.287035094753648
1102	17	1.2938154846600085
1102	18	0.7446490814011142
1102	19	1.0549018425784027
1102	20	1.2195951758918824
1102	21	1.1904503994219837
1102	22	0.9506268176554684
1102	23	1.1802450286217265
1102	24	1.2256919639279005
1102	25	1.3161507635475758
1103	1	0.5528039661756203
1103	2	0.8777379032046149
1103	3	0.4886324276276035
1103	4	0.38593971209778744
1103	5	0.5489146490388706
1103	6	0.6261839686251633
1103	7	0.663942729663006
1103	8	0.6922066784271195
1103	9	0.7492060528391136
1103	10	0.6147816141550138
1103	11	0.8673949164909587
1103	12	0.9003386932046311
1103	13	0.8944071723317748
1103	14	0.9422329511018077
1103	15	1.0737559816815434
1103	16	0.9800255089589669
1103	17	1.0249224525300562
1103	18	1.0068050204843573
1103	19	1.0528606479518814
1103	20	0.8048618019543525
1103	21	0.9625592382890318
1103	22	0.9015621036518979
1103	23	1.0895798422755902
1103	24	1.1311129513291576
1103	25	1.0635717267563152
1104	1	0.6897922658573492
1104	2	0.7535938858534976
1104	3	0.6863689621169904
1104	4	0.4803561376162122
1104	5	0.6543066318521227
1104	6	0.5973831308610542
1104	7	0.48158842504638244
1104	8	0.5690943924829739
1104	9	0.6225129802519405
1104	10	0.7095650430166529
1104	11	0.831509809853884
1104	12	0.7979543271874903
1104	13	0.624008855564739
1104	14	0.9210830672189445
1104	15	0.9209046751791803
1104	16	0.852175952078909
1104	17	0.8441279763284513
1104	18	0.33320839414140124
1104	19	0.8899035230083072
1104	20	0.7920673437305155
1104	21	0.8474839146410034
1104	22	0.6636715767170216
1104	23	0.6069866901367789
1104	24	0.785193038444298
1104	25	0.787705727921022
1105	1	0.6153056086443129
1105	2	0.6713200285527208
1105	3	0.6605743422804622
1105	4	0.4373389678521704
1105	5	0.43633818654009815
1105	6	0.5434356943335246
1105	7	0.6215316366527119
1105	8	0.6508988140956689
1105	9	0.8068759032373265
1105	10	0.5258887209735192
1105	11	0.8472851039046105
1105	12	0.9263923926819686
1105	13	0.919151298034528
1105	14	1.0122987519057602
1105	15	1.0451211506293845
1105	16	0.9985190685681431
1105	17	0.8009169593493368
1105	18	0.6682374955666717
1105	19	0.9621549796170257
1105	20	0.9776442140806836
1105	21	0.8585570364242017
1105	22	0.7783549459328043
1105	23	1.0952346546769647
1105	24	0.9851239555504634
1105	25	0.8293199717067594
1106	1	0.11322036104157007
1106	2	0.4308664349023559
1106	3	-1.6254650499412406
1106	4	-0.2860020081321011
1106	5	0.05191365297481809
1106	6	0.11492062355222998
1106	7	0.2996097097289123
1106	8	0.40651197879580536
1106	9	0.5762818407928165
1106	10	0.3705542013286873
1106	11	0.44305999710116595
1106	12	0.6226359698518067
1106	13	0.5432170055970289
1106	14	0.7064934105137581
1106	15	0.6630727592976768
1106	16	0.6029260435341541
1106	17	0.6177202949061282
1106	18	0.7725913540649216
1106	19	0.7938763046591149
1106	20	0.8176214120335601
1106	21	0.7536111588260539
1106	22	0.6690999457059092
1106	23	0.7841501265728397
1106	24	0.7332456654443149
1106	25	0.7490116758587888
1107	1	0.6787700739040403
1107	2	0.7887806942645454
1107	3	0.7107843451369256
1107	4	0.5029053508709822
1107	5	0.6177861205925979
1107	6	0.610021486684353
1107	7	0.5861428681404988
1107	8	0.5657584872499157
1107	9	0.6482954086915527
1107	10	0.6665111626893534
1107	11	0.8119647242319346
1107	12	0.9853533961817647
1107	13	0.7227860326127811
1107	14	0.8638167574403397
1107	15	0.7342116319608678
1107	16	0.7979023599277753
1107	17	0.8281940306243598
1107	18	0.9348805431043417
1107	19	0.9218510308983241
1107	20	0.8253694728121559
1107	21	0.9624343939499767
1107	22	0.9029197385017724
1107	23	1.0657045300858456
1107	24	0.9752711766965447
1107	25	1.046118134704443
1108	1	0.4122832704433961
1108	2	0.41693920462867595
1108	3	0.39195124814899174
1108	4	0.25920282485949997
1108	5	0.46263256663426233
1108	6	0.36744806502134253
1108	7	0.24647344459501852
1108	8	0.26707670941791406
1108	9	0.3723753735323214
1108	10	0.31111221622127516
1108	11	0.2531709946268421
1108	12	0.38762679406499245
1108	13	0.3739226693036315
1108	14	0.49575874470881587
1108	15	0.52712351102506
1108	16	0.42491234370522335
1108	17	0.4541915679841537
1108	18	0.5730701414997696
1108	19	0.5336129391581323
1108	20	0.5512187288532857
1108	21	0.6091217195700267
1108	22	0.5225201384006724
1108	23	0.5451592880984535
1108	24	0.5944955819838782
1108	25	0.49500515330372963
1109	1	0.2247738728091342
1109	2	0.1741797035814443
1109	3	0.26880826747208886
1109	4	0.1294517709206673
1109	5	-0.06700924078114667
1109	6	0.1999310931278302
1109	7	0.24399059742223272
1109	8	0.20989317338683833
1109	9	0.41499256086172664
1109	10	-0.04281158074115865
1109	11	0.21031759522567484
1109	12	0.5446449317330817
1109	13	0.443919306432349
1109	14	0.5940027519037372
1109	15	0.5044024178360971
1109	16	0.4709202286713179
1109	17	0.5167572578375825
1109	18	0.5597077506293076
1109	19	0.6029564056108399
1109	20	0.6775774006002351
1109	21	0.7716071746838011
1109	22	0.6763511600459609
1109	23	0.6362247817323947
1109	24	0.7031415302854995
1109	25	0.6491746457597287
1110	1	0.4917545673871402
1110	2	0.2171910563888524
1110	3	0.2956170216804246
1110	4	0.31990714346744653
1110	5	0.3557527438586945
1110	6	0.46354095933642014
1110	7	0.431937823350367
1110	8	0.3726360121536274
1110	9	0.313398126822797
1110	10	0.4973055418402268
1110	11	0.40859570827696245
1110	12	0.5053777021545685
1110	13	0.4662965732534303
1110	14	0.4907458427206137
1110	15	0.6152190290538115
1110	16	0.4973713945891802
1110	17	0.6124373926285003
1110	18	0.5777693116884421
1110	19	0.576439495595821
1110	20	0.6519037408889545
1110	21	0.700969643185303
1110	22	0.687928577600438
1110	23	0.7053277255655814
1110	24	0.6997225889082515
1110	25	0.6543617246545068
1111	1	0.19864208315417287
1111	2	0.0033006609072110393
1111	3	-0.005336828982763109
1111	4	0.15654452865150859
1111	5	0.36357694974513777
1111	6	0.30434597672869046
1111	7	0.09366893391311493
1111	8	0.18207681641946039
1111	9	0.21387661789743362
1111	10	0.1477447640792917
1111	11	0.1692063731165092
1111	12	0.36741306790587913
1111	13	0.27764795981284607
1111	14	0.18134299206619175
1111	15	0.19601299856316245
1111	16	0.17159989036105117
1111	17	0.3171512812703483
1111	18	0.25957938580445017
1111	19	0.045067140605546285
1111	20	0.21602805838156058
1111	21	0.31039554157734983
1111	22	0.21322422120225326
1111	23	0.32119433928925645
1111	24	0.30364358527197055
1111	25	0.20275458336310948
1112	1	-0.02726547076889929
1112	2	-0.38909658529350466
1112	3	-0.11487277220214054
1112	4	-0.07973124699603318
1112	5	-0.04777232565761835
1112	6	0.003030591118154291
1112	7	0.1057367185708813
1112	8	-0.03131675415932733
1112	9	0.03846016156224863
1112	10	-0.23274171678540512
1112	11	-0.08302056165832283
1112	12	0.3154397992404938
1112	13	0.15158592322912057
1112	14	-0.038461608929701185
1112	15	-0.30353915392061026
1112	16	-0.3017245815695304
1112	17	-0.15399838427088497
1112	18	1.0368828606743818E-4
1112	19	-1.52521561929575E-4
1112	20	-0.061253858522498206
1112	21	-0.14708951114715063
1112	22	-0.0511969507859078
1112	23	0.2703557191314232
1112	24	0.15351637171808363
1112	25	0.09429900410385983
1113	1	-0.4609309908477499
1113	2	-0.3935166354003954
1113	3	-0.42259465400604057
1113	4	-1.8829171950176757
1113	5	-0.5619384815874398
1113	6	-0.3695856239346469
1113	7	-0.28809539577467547
1113	8	-0.09770681362353173
1113	9	-0.152973633162226
1113	10	-0.3237924260643652
1113	11	-0.5011049113251715
1113	12	-0.02415232434425718
1113	13	-0.2592099600787776
1113	14	-0.3574201388475089
1113	15	-0.4438416719954503
1113	16	-0.6753201646035265
1113	17	-0.797117544973851
1113	18	-0.6217811997214824
1113	19	-0.3211791091879661
1113	20	-0.04542097249402843
1113	21	-0.20466296210526025
1113	22	-0.17561093766992286
1113	23	-0.033642792622963213
1113	24	0.04278840438074383
1113	25	-0.21064206320709644
1114	1	-0.8628012803477674
1114	2	-2.152113550651265
1114	3	-1.3906343233205654
1114	4	-0.9059455597100481
1114	5	-0.8141384483062666
1114	6	-0.4567253234102395
1114	7	-0.45817326275165726
1114	8	-0.4487304265033458
1114	9	-0.7967168295881919
1114	10	-0.9110322290813997
1114	11	-0.9207846031044937
1114	12	-0.919746641814335
1114	13	-1.0846383396783423
1114	14	-1.0236330413128556
1114	15	-1.0486207566243948
1114	16	-1.4340468677793368
1114	17	-1.4427924652954758
1114	18	-1.181042640704014
1114	19	-1.7768801864349655
1114	20	-1.1202646202959627
1114	21	-1.325059282570642
1114	22	-1.0378342301028454
1114	23	-0.9108784383818076
1114	24	-0.9093421687719783
1114	25	-1.4659399673018356
1115	1	-1.0062492676784558
1115	2	-1.2110975543338007
1115	3	-0.7071737895527797
1115	4	-0.5400582555941398
1115	5	-0.8619094051911773
1115	6	-0.7070623744143276
1115	7	-0.6167649212889756
1115	8	-0.8379845730961293
1115	9	-1.8555347637267303
1115	10	-1.4308424544744156
1115	11	-1.8246189539581366
1115	12	-2.4866224573483393
1115	13	-2.416527621659121
1115	14	-1.9429990267773292
1115	15	-2.17216527012328
1115	16	-1.8255333632846984
1115	17	-1.7312792770346803
1115	18	-2.0834020133260225
1115	19	-2.597380060440422
1115	20	-2.9144023186126304
1115	21	-2.159233541136402
1115	22	-2.1329767972978573
1115	23	-2.0823524934850823
1115	24	-2.588532610152079
1115	25	-2.5723592802697937
1116	1	-1.089363077672175
1116	2	-1.1544293249973236
1116	3	-1.2831823936634024
1116	4	-0.7945155085601527
1116	5	-1.2391621070879708
1116	6	-1.9537267927965551
1116	7	-1.3466168521994533
1116	8	-1.7642925034315375
1116	9	-2.870068340426265
1116	10	-1.4825102234814977
1116	11	-2.44657951861722
1116	12	-4.620673489252344
1116	13	-3.6867303821155453
1116	14	-2.991163807505437
1116	15	-4.243702076354257
1116	16	-3.600616300437842
1116	17	-3.185390541699654
1116	18	-3.126047949265363
1116	19	-3.3692486651166433
1116	20	-5.237552052985755
1116	21	-4.0256118177752995
1116	22	-3.2518110464520404
1116	23	-4.229088807967173
1116	24	-4.002041577106908
1116	25	-3.296612135775824
1201	1	0.4401032921575485
1201	2	0.641927355451724
1201	3	0.7125765946426554
1201	4	0.603361988146105
1201	5	0.08189942723850407
1201	6	0.6210861743475462
1201	7	0.7706253302786408
1201	8	0.6883600065939532
1201	9	0.5880239997858823
1201	10	0.6577482694927994
1201	11	0.3998656510600398
1201	12	0.8240435502184909
1201	13	0.9459611166692241
1201	14	0.965159390596078
1201	15	0.8870275442278057
1201	16	0.7741026114933263
1201	17	0.6567178322863896
1201	18	0.8982505818870337
1201	19	1.023631943985471
1201	20	1.0922753524278406
1201	21	0.8062615688629933
1201	22	0.7559288845764698
1201	23	0.9560145844333476
1201	24	0.6801324019086863
1201	25	0.7427716740887362
1202	1	0.7924162712746501
1202	2	0.8107592297293245
1202	3	0.752565399046258
1202	4	0.6874628936417935
1202	5	0.7605312838933997
1202	6	0.8123231322552158
1202	7	0.8576764827512591
1202	8	0.726361177985055
1202	9	1.09649937724852
1202	10	1.1520738643884414
1202	11	1.0639958931648152
1202	12	1.1135851009122533
1202	13	1.162231635450354
1202	14	1.3199727195755742
1202	15	1.2483703938180781
1202	16	1.290116979175373
1202	17	1.184123926630022
1202	18	1.263090189419188
1202	19	1.3891117737709422
1202	20	1.288660418746197
1202	21	1.3305306156305292
1202	22	1.25578468944137
1202	23	1.4481844050446497
1202	24	1.3534237517555283
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2211	10	-0.011470334588288722
2211	11	0.050183454162059604
2211	12	-0.06455111888486442
2211	13	0.047219066320138836
2211	14	-0.13264018078819362
2211	15	-0.08935651595034955
2211	16	-0.08858943551719989
2211	17	-0.18213875340428132
2211	18	-0.2546278480220394
2211	19	-0.10836907678949359
2211	20	-0.07669491795861916
2211	21	-0.07129116889021958
2211	22	-0.12248638972936021
2211	23	-0.24370333044170422
2211	24	0.06361450679057157
2211	25	-0.13429171365849157
2212	1	-0.30254384792948485
2212	2	-0.24539444323955806
2212	3	-0.11798860114966558
2212	4	-0.258653467882219
2212	5	-0.6075854591663443
2212	6	-0.3215429074554379
2212	7	-0.46657203793845525
2212	8	-0.2821952592273931
2212	9	-0.3543875902126672
2212	10	-0.5477667499184662
2212	11	-0.32944602317574123
2212	12	-0.20572036656354697
2212	13	-0.11771859304143106
2212	14	-0.12413590467875935
2212	15	-0.3967413600604388
2212	16	-0.3000424924491938
2212	17	-0.33731841593063194
2212	18	-0.47818011833578566
2212	19	-0.2514691229755215
2212	20	-0.36842949478955234
2212	21	-0.26915143792540164
2212	22	-0.23225732761086704
2212	23	-0.6689494710318584
2212	24	-0.5827253152864884
2212	25	-0.4780404079181224
2213	1	-0.6356529649851623
2213	2	-0.2328479976012936
2213	3	-0.3259799677085802
2213	4	-0.6482774039047783
2213	5	-0.4394440138631026
2213	6	-0.2879814820958799
2213	7	-0.607507769393564
2213	8	-0.6336017336606901
2213	9	-0.49205375706730337
2213	10	-0.4177742133151625
2213	11	-0.3578712605489187
2213	12	-0.7786594443005512
2213	13	-0.5898234792436341
2213	14	-0.8027778367497405
2213	15	-1.0105482357322373
2213	16	-1.5346531430062598
2213	17	-1.0287073143603038
2213	18	-0.6197151597011938
2213	19	-0.6034847609171621
2213	20	-0.6935671917840978
2213	21	-0.523136232979013
2213	22	-0.7335466005335718
2213	23	-0.8411254016760381
2213	24	-0.5822946426686286
2213	25	-0.6403095310773992
2214	1	-1.3538904403863903
2214	2	-0.5588031043906767
2214	3	-0.7149521906667928
2214	4	-0.7681008226583756
2214	5	-1.070164658525563
2214	6	-1.7804636517497272
2214	7	-1.1970774083064697
2214	8	-1.0429825069955
2214	9	-0.8887051927684624
2214	10	-1.1510226603106801
2214	11	-1.1959595535808134
2214	12	-0.9366338423080762
2214	13	-1.3172913653510179
2214	14	-0.936332007039482
2214	15	-1.067355140490129
2214	16	-1.088312187766995
2214	17	-1.2310407954267788
2214	18	-1.1684047707696124
2214	19	-1.2277808205371201
2214	20	-1.285779467444499
2214	21	-1.4523068238071914
2214	22	-1.9322125461982935
2214	23	-2.043274015593944
2214	24	-1.7691354136429212
2214	25	-2.2219235597841944
2215	1	-1.617896804231794
2215	2	-1.0644586383619874
2215	3	-1.1419164669772677
2215	4	-1.214663541501423
2215	5	-1.62327370577718
2215	6	-1.4938668796611552
2215	7	-1.9793428666188042
2215	8	-1.703157054819357
2215	9	-1.6364453342214063
2215	10	-1.8220812612655806
2215	11	-1.4434482452931263
2215	12	-1.7669697039310748
2215	13	-1.9866379819904978
2215	14	-2.1874000347020655
2215	15	-2.0632550787030013
2215	16	-2.0063132416480514
2215	17	-2.2006530738280077
2215	18	-1.8271537825516901
2215	19	-2.042142713872714
2215	20	-2.0782855128686677
2215	21	-1.7102590956208417
2215	22	-2.0565046027415264
2215	23	-2.4398857323832033
2215	24	-2.3891482453480535
2215	25	-2.62231364623711
2216	1	-1.9264820442300845
2216	2	-1.170619563519196
2216	3	-1.2182703391590195
2216	4	-1.4899909557204012
2216	5	-2.447812548905919
2216	6	-2.6973696950785957
2216	7	-2.4980204479329764
2216	8	-2.653299222754608
2216	9	-4.648371265955273
2216	10	-2.61825838162936
2216	11	-2.826001141429181
2216	12	-3.302145823450605
2216	13	-3.0198363856594774
2216	14	-3.5522790361617638
2216	15	-3.4739903586717773
2216	16	-2.586134458323272
2216	17	-2.861856839216216
2216	18	-3.112396751945333
2216	19	-3.473667366164875
2216	20	-2.6493533378445946
2216	21	-3.4491247704388144
2216	22	-2.7246066959336304
2216	23	-4.1517046935924675
2216	24	-3.264711803462504
2216	25	-3.308164716194419
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	52263.0
3	3.0
4	863.0
5	7373.0
6	8864.0
7	7130.0
8	5645.0
9	4997.0
10	4327.0
11	3847.0
12	3548.0
13	3402.0
14	3347.0
15	3357.0
16	3531.0
17	3775.0
18	4207.0
19	5153.0
20	6537.0
21	8918.0
22	11501.0
23	14200.0
24	17041.0
25	19574.0
26	21992.0
27	24400.0
28	27906.0
29	33674.0
30	42526.0
31	54939.0
32	68895.0
33	88753.0
34	116716.0
35	152201.0
36	208174.0
37	322097.0
38	1038057.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.52652004743694	19.250186549762148	13.45073753780964	26.772555864991272
2	13.621934739007221	20.079361016460293	40.84024953687344	25.45845470765904
3	17.534394546138458	25.124537716661504	29.469376953573605	27.87169078362643
4	11.878030332657406	16.727602684956903	36.906217054818285	34.488149927567406
5	13.043858178173146	38.701327233863545	34.71579752412235	13.53901706384096
6	32.40816635771338	36.22030166551703	17.87713497269375	13.494397004075854
7	28.90778071346314	31.358920704020466	21.45402192132604	18.279276661190362
8	26.062556896489287	36.75474704554508	19.93132669188878	17.25136936607685
9	27.55815767956008	14.362060033331044	19.62191526590975	38.45786702119913
10	16.408829936808914	28.424876194249954	33.048664046677	22.11762982226414
11	34.75502320482444	21.738354423270142	24.621743679779666	18.884878692125753
12	24.583022010846502	24.269758092027203	30.719417037896196	20.427802859230095
13	29.58513930342877	21.57595142623446	25.565879875287173	23.273029395049598
14	21.372393459618515	22.094276646171984	26.963119141095465	29.570210753114033
15	23.428799438795274	31.18006524920311	22.72929070096917	22.66184461103245
16	22.46143579177907	27.26176231233805	27.674947285735445	22.601854610147434
17	21.884112538353527	28.51114462530553	27.26452753653336	22.340215299807582
18	21.868696954568147	26.370860376102513	30.063030454318522	21.697412215010818
19	24.205181029849083	25.983154247715596	27.25619588532974	22.555468837105586
20	24.892707076590256	26.170279152972505	27.546906851936598	21.390106918500646
21	23.815694774371497	25.76924196977198	26.644132244470054	23.77093101138647
22	24.38087733866284	27.57754218038344	26.435522547513774	21.606057933439946
23	22.56340450457684	27.307109400253687	27.645624534838102	22.483861560331366
24	24.02750222258462	26.008296262521668	27.995164188368676	21.969037326525033
25	23.035762722300156	26.76658629164982	27.8996993009204	22.297951685129625
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	83.0
1	83.0
2	251.0
3	419.0
4	419.0
5	419.0
6	1080.5
7	1742.0
8	1742.0
9	1742.0
10	2180.0
11	2618.0
12	2618.0
13	2618.0
14	4333.0
15	6048.0
16	6048.0
17	6048.0
18	12903.0
19	19758.0
20	19758.0
21	19758.0
22	34852.5
23	49947.0
24	49947.0
25	49947.0
26	77522.5
27	105098.0
28	105098.0
29	105098.0
30	137869.5
31	170641.0
32	170641.0
33	170641.0
34	194492.5
35	218344.0
36	218344.0
37	218344.0
38	236817.0
39	255290.0
40	255290.0
41	255290.0
42	271978.0
43	288666.0
44	288666.0
45	288666.0
46	306684.0
47	324702.0
48	324702.0
49	324702.0
50	315575.5
51	306449.0
52	306449.0
53	306449.0
54	272452.5
55	238456.0
56	238456.0
57	238456.0
58	208414.5
59	178373.0
60	178373.0
61	178373.0
62	148465.0
63	118557.0
64	118557.0
65	118557.0
66	91912.0
67	65267.0
68	65267.0
69	65267.0
70	48678.0
71	32089.0
72	32089.0
73	32089.0
74	22978.5
75	13868.0
76	13868.0
77	13868.0
78	9508.5
79	5149.0
80	5149.0
81	5149.0
82	3381.0
83	1613.0
84	1613.0
85	1613.0
86	1034.5
87	456.0
88	456.0
89	456.0
90	269.0
91	82.0
92	82.0
93	82.0
94	49.5
95	17.0
96	17.0
97	17.0
98	9.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09273076502257115
2	0.04334924053545049
3	0.018512871437884326
4	0.00495063303619828
5	0.019303308645344554
6	0.06390060792941646
7	0.07783726395568892
8	0.05591303193823939
9	0.023629912307232125
10	0.049048708820821615
11	0.005741070243658509
12	3.744176245864245E-4
13	0.005449856535646846
14	8.320391657476101E-4
15	0.01381185015141033
16	0.008694809282062526
17	0.0044930114950370945
18	0.005533060452221608
19	0.0017056802897826007
20	0.0013728646234835566
21	1.2480587486214152E-4
22	3.744176245864245E-4
23	0.0
24	0.0
25	4.16019582873805E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2403733.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.44466980774717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.6756960192914	33.05740391260244
2	15.557478386552523	14.76238850182295
3	6.047764868197756	8.608026217396084
4	2.895512124521936	5.495064666890871
5	1.630132292382072	3.8670544177506696
6	1.004873333909236	2.860553011556026
7	0.6685744675001248	2.220420639670367
8	0.47587121935362314	1.8062042298594139
9	0.3316094749003498	1.4159791837590767
>10	1.5683031671964343	12.898890508364824
>50	0.08463723542718189	2.735998850420836
>100	0.05084676407568705	4.685256721446702
>500	0.004570036646194427	1.4716503864822572
>1k	0.0036911834450022154	2.830719143125957
>5k	4.3942660059550186E-4	1.2843896088514737
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6686	0.27815069310942603	No Hit
GTCCTACAGTGGACATTTCTAAATT	6565	0.27311685615665304	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6080	0.2529399063872735	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5896	0.24528514606239546	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5576	0.23197251941043368	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4579	0.19049536699791533	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3812	0.15858666499149449	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2939	0.1222681554066113	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2774	0.11540383228919353	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2524	0.1050033427173484	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.16019582873805E-5
7	0.0	0.0	0.0	0.0	4.16019582873805E-5
8	0.0	0.0	0.0	0.0	4.16019582873805E-5
9	0.0	0.0	0.0	0.0	4.16019582873805E-5
10	0.0	0.0	0.0	0.0	4.16019582873805E-5
11	0.0	0.0	0.0	0.0	4.16019582873805E-5
12	0.0	0.0	0.0	0.0	4.16019582873805E-5
13	0.0	0.0	0.0	0.0	4.16019582873805E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1745	0.0	13.300297	1
CCCGTAC	140	3.6379788E-12	12.882679	16
TAGGACC	860	0.0	12.827854	4
GCCCCGT	130	2.6466296E-9	11.684051	14
GGCGAGG	1460	0.0	11.507553	19
CCCCGTA	165	7.2759576E-12	11.507019	15
AGGACCT	1845	0.0	11.391748	5
CCGTACA	160	4.5474735E-11	11.272345	17
CACCTTT	875	0.0	10.957971	14
TATGTCG	165	8.185452E-11	10.930758	16
TCCAACG	115	8.9124114E-7	10.730653	18
GTATCAA	3860	0.0	10.71936	1
CGAATTT	80	3.7969957E-4	10.679952	15
TCGAATT	80	3.7969957E-4	10.679952	14
GGACCTG	1945	0.0	10.561354	6
AATCCCG	90	9.599677E-5	10.546784	19
CCACCTT	940	0.0	10.503216	13
TAGAAAT	855	0.0	10.455775	4
CGCCCTA	310	0.0	10.41113	16
TCCCGCC	330	0.0	10.356317	13
>>END_MODULE