FastQCFastQC Report
Thu 26 May 2016
SRR1512893_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512893_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843221
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82250.28928458252102107No Hit
GTCCTACAGTGGACATTTCTAAATT50520.17768580071686302No Hit
TATCAACGCAGAGTACTTTTTTTTT46170.16238625136772697No Hit
GGTATCAACGCAGAGTACTTTTTTT45510.16006494043199596No Hit
CTGTAGGACGTGGAATATGGCAAGA42290.14873975677585385No Hit
GTCCTAAAGTGTGTATTTCTCATTT38540.13555049009556414No Hit
CTTTAGGACGTGAAATATGGCGAGG32100.11290012278327995No Hit
GTCCTACAGTGTGCATTTCTCATTT28720.10101219708211215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20100.014.0255061
TAGGACC7750.012.2560314
CGTACTG550.003073336612.0889045
TAGAAAT6600.011.5132414
GTATCAA47350.011.2237281
TTGCGCA600.005884948711.0814959
GTCCTAC24800.010.7913941
ACACCGT1158.8412526E-710.7375386
GTCCTAT3600.010.5823091
ATTTCTA15650.010.43953115
GTCCTAA12700.010.423991
TCCTACA26500.010.3945022
TGTAGGA25900.010.2685672
TAAGACT2700.010.2020114
GTAGGAC24650.010.0186223
GTACGCT951.647665E-49.99834253
CTTACAC3800.09.99834253
CCTACAG25950.09.9925623
GACGTGG11700.09.9856997
CTAATAC2300.09.91143
Produced by FastQC (version 0.11.5)