##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512893_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2843221 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.67896164244707 31.0 30.0 34.0 18.0 34.0 2 28.861637558248198 31.0 30.0 34.0 16.0 34.0 3 28.95497958125661 31.0 30.0 34.0 20.0 34.0 4 32.39030803444403 35.0 33.0 37.0 22.0 37.0 5 32.56450624133685 35.0 33.0 37.0 25.0 37.0 6 32.45031603241535 36.0 33.0 37.0 23.0 37.0 7 32.38747075939577 36.0 33.0 37.0 22.0 37.0 8 32.28653242220707 36.0 33.0 37.0 22.0 37.0 9 33.58378191494787 38.0 34.0 39.0 19.0 39.0 10 33.45545527414155 38.0 33.0 39.0 18.0 39.0 11 33.50739706832498 38.0 34.0 39.0 18.0 39.0 12 33.3963842416752 38.0 34.0 39.0 17.0 39.0 13 33.369094417915456 38.0 34.0 39.0 17.0 39.0 14 34.208537781621615 39.0 33.0 41.0 17.0 41.0 15 34.372660795625805 39.0 34.0 41.0 17.0 41.0 16 34.317391437387386 39.0 34.0 41.0 17.0 41.0 17 34.06891233569251 39.0 33.0 41.0 16.0 41.0 18 34.07228597425244 39.0 33.0 41.0 16.0 41.0 19 34.18080901906676 39.0 34.0 41.0 16.0 41.0 20 34.14567175748913 39.0 34.0 41.0 14.0 41.0 21 34.04340851449817 39.0 34.0 41.0 10.0 41.0 22 34.04925294234954 39.0 34.0 41.0 10.0 41.0 23 33.70894453860604 39.0 33.0 41.0 2.0 41.0 24 33.770864452675326 39.0 34.0 41.0 2.0 41.0 25 33.48646833995669 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5577043594941529 1101 2 0.2671085196238181 1101 3 0.619205066682671 1101 4 0.6543152751876065 1101 5 0.5399294721182457 1101 6 0.4720724626159125 1101 7 0.17115320288211677 1101 8 0.5951913189456164 1101 9 0.6281826876162953 1101 10 0.7347665995800483 1101 11 0.6063984359918351 1101 12 0.4866367293147462 1101 13 1.0170262767599993 1101 14 1.0442366097555222 1101 15 0.8680174052850518 1101 16 0.7596088254415747 1101 17 0.12211459237904876 1101 18 0.6361451357519599 1101 19 0.12146450154924793 1101 20 -0.45032007870846513 1101 21 0.5300947816875379 1101 22 0.5467023169562921 1101 23 0.0695164147117211 1101 24 0.5658160442022862 1101 25 0.23797777825668476 1102 1 1.0668908121035763 1102 2 1.0886224005390943 1102 3 1.0238216157955478 1102 4 1.163405190166145 1102 5 0.9902715399713813 1102 6 0.9825446597782204 1102 7 0.9739746646497949 1102 8 0.9591398959980282 1102 9 1.3632372954513912 1102 10 1.3392553431214296 1102 11 1.0183364673759598 1102 12 1.1893461049127652 1102 13 1.050237784729056 1102 14 1.2711060225333455 1102 15 1.2220704480144846 1102 16 1.275173596211694 1102 17 1.3940585356251702 1102 18 1.3607950373348388 1102 19 1.1556815386470376 1102 20 1.3068920512021407 1102 21 1.396442276151575 1102 22 1.2805258564188193 1102 23 1.1538911584171245 1102 24 0.6567698701680058 1102 25 0.9959323081782188 1103 1 0.48774246414846445 1103 2 0.692320311500616 1103 3 0.7492798325364021 1103 4 0.8734106505114241 1103 5 0.6906996127848402 1103 6 0.4348166364232142 1103 7 0.3134941418175856 1103 8 0.45566639443958934 1103 9 0.5601209154113604 1103 10 0.6648401519097646 1103 11 0.5105715534967246 1103 12 0.5379413315087973 1103 13 0.7076922970654493 1103 14 0.6946276838392507 1103 15 0.5981651103195205 1103 16 0.6150864174011517 1103 17 0.26123522177647374 1103 18 0.6403171036025199 1103 19 0.6183399965393122 1103 20 0.8105815185446232 1103 21 0.5709260328691315 1103 22 0.6185742352439689 1103 23 -0.05184301322027807 1103 24 0.10451756072409779 1103 25 0.5263149182232638 1104 1 1.128371000968098 1104 2 0.9410378288393169 1104 3 0.9193526540594377 1104 4 1.052385092624334 1104 5 0.8811216758098155 1104 6 0.839927805992744 1104 7 1.0842838607481227 1104 8 0.8659142241705879 1104 9 1.1300207067963584 1104 10 1.0607491158293598 1104 11 0.8138936913620824 1104 12 1.1364834735750051 1104 13 1.087022791766465 1104 14 1.3965587491216738 1104 15 1.1074786588750172 1104 16 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2215 16 -2.1099326979696187 2215 17 -2.3879937796480313 2215 18 -2.042052858080666 2215 19 -2.1089215244806283 2215 20 -1.9561250900760712 2215 21 -2.6251167216214704 2215 22 -2.510921616961376 2215 23 -2.4487647981030385 2215 24 -2.317631492647493 2215 25 -3.2946486030298914 2216 1 -3.047094139333531 2216 2 -3.153847754545666 2216 3 -4.434449283063074 2216 4 -4.939631062215842 2216 5 -4.602688241512077 2216 6 -3.6752167490402456 2216 7 -5.1965458369359965 2216 8 -3.074125109705559 2216 9 -3.858576114896824 2216 10 -2.5895650285676908 2216 11 -2.9623482018490783 2216 12 -3.3661552583429604 2216 13 -3.0352032787752528 2216 14 -3.932363397349416 2216 15 -3.3366334122905386 2216 16 -3.6589596814045393 2216 17 -3.3569239316204253 2216 18 -2.906773776583229 2216 19 -3.656714197840742 2216 20 -4.4350108361965646 2216 21 -3.9219100801213784 2216 22 -3.5422832498656724 2216 23 -3.6293394362063367 2216 24 -4.012662458279369 2216 25 -4.0167241695118605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 152406.0 3 6.0 4 1391.0 5 8398.0 6 9865.0 7 8721.0 8 8102.0 9 7361.0 10 6609.0 11 6294.0 12 5836.0 13 5663.0 14 5558.0 15 5925.0 16 6168.0 17 6495.0 18 7342.0 19 8777.0 20 11123.0 21 14744.0 22 18352.0 23 21722.0 24 25413.0 25 28769.0 26 31801.0 27 35238.0 28 39300.0 29 47055.0 30 56428.0 31 69715.0 32 87141.0 33 109123.0 34 140972.0 35 183584.0 36 247720.0 37 374916.0 38 1039188.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.41019688052823 17.795012896343323 12.351023326342595 24.44376689678585 2 15.428608613678893 19.248483953118626 41.138793916114416 24.184113517088065 3 19.902579997945793 24.486483748559593 28.12907587476099 27.481860378733625 4 12.725684087302033 15.358723731160161 36.9011773843158 35.014414797222 5 13.811716262690965 38.25406657851628 33.91485854606737 14.019358612725382 6 33.25608261415307 34.85729853859568 17.792715576321573 14.093903270929678 7 29.878972533692266 31.200651318123708 20.492726525233298 18.427649622950728 8 26.82051029281809 35.570831558304846 19.67348864392518 17.93516950495189 9 27.99878066294764 14.77200086733444 19.19611051192444 38.033107957793476 10 16.96705825117287 28.048907990724757 32.140391687767824 22.843642070334543 11 35.53738179585814 22.143740178988807 23.20623982698124 19.112638198171812 12 24.740336116999163 24.073094199293855 29.893303958212975 21.293265725494006 13 29.776737738738767 21.323029625272667 25.16766581518164 23.73256682080693 14 22.16829732094895 21.300578502679894 26.491965944588497 30.039158231782658 15 24.095453790244687 29.745972363415174 22.777372581376422 23.381201264963718 16 23.309689841981687 27.327417180311247 26.315381880519993 23.04751109718707 17 22.63945022602132 27.86114123164017 26.58035374801087 22.91905479432764 18 22.540136199558443 26.49515506843952 28.550400279398154 22.414308452603883 19 24.233514457358403 26.09685646845115 26.95132533852943 22.718303735661014 20 24.954279331371083 25.87833848889978 27.148836221964295 22.01854595776484 21 24.58313958870641 25.673905705120738 26.213023229291444 23.529931476881412 22 24.61915333923395 26.757210825867787 26.29716707118972 22.326468763708544 23 23.655859469472546 26.499582659621694 26.981909148291194 22.86264872261457 24 24.094743752893198 25.612354636068734 27.462311425116564 22.8305901859215 25 23.623822045741765 26.250435627461954 27.30023620689048 22.8255061199058 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 307.0 1 307.0 2 762.0 3 1217.0 4 1217.0 5 1217.0 6 2057.5 7 2898.0 8 2898.0 9 2898.0 10 3385.5 11 3873.0 12 3873.0 13 3873.0 14 5919.0 15 7965.0 16 7965.0 17 7965.0 18 15351.0 19 22737.0 20 22737.0 21 22737.0 22 37344.5 23 51952.0 24 51952.0 25 51952.0 26 78215.0 27 104478.0 28 104478.0 29 104478.0 30 138071.0 31 171664.0 32 171664.0 33 171664.0 34 199803.5 35 227943.0 36 227943.0 37 227943.0 38 254793.5 39 281644.0 40 281644.0 41 281644.0 42 306086.0 43 330528.0 44 330528.0 45 330528.0 46 355345.0 47 380162.0 48 380162.0 49 380162.0 50 378512.0 51 376862.0 52 376862.0 53 376862.0 54 344858.5 55 312855.0 56 312855.0 57 312855.0 58 274367.5 59 235880.0 60 235880.0 61 235880.0 62 198832.0 63 161784.0 64 161784.0 65 161784.0 66 126752.0 67 91720.0 68 91720.0 69 91720.0 70 68859.0 71 45998.0 72 45998.0 73 45998.0 74 33009.0 75 20020.0 76 20020.0 77 20020.0 78 13663.0 79 7306.0 80 7306.0 81 7306.0 82 4912.0 83 2518.0 84 2518.0 85 2518.0 86 1625.0 87 732.0 88 732.0 89 732.0 90 432.5 91 133.0 92 133.0 93 133.0 94 81.0 95 29.0 96 29.0 97 29.0 98 22.5 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33314329065521114 2 0.010586584722045876 3 0.009601786143250912 4 0.010867955744558724 5 0.012380324990565277 6 0.07931145697080881 7 0.07783425910261636 8 0.10168045326058017 9 0.08205482444030907 10 0.08933529964782899 11 0.08346167955287331 12 0.09573649040999627 13 0.08096451172807179 14 0.0857829904886043 15 0.08261756648533476 16 0.08479819190980933 17 0.09781160170102851 18 0.09953499921391971 19 0.0923600381398421 20 0.09151592507230355 21 0.08026108417178968 22 0.09985154161424666 23 0.09193798160607283 24 0.0881394728021494 25 0.0876119021349378 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2843221.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.31211608307578 #Duplication Level Percentage of deduplicated Percentage of total 1 72.23499794071647 36.34295601654066 2 14.084792241377627 14.172714045083923 3 5.453209768518097 8.230875686971359 4 2.6735172526274864 5.380412414571996 5 1.5114129937530956 3.802119299558741 6 0.9647754346745764 2.91239361980683 7 0.6229105858645765 2.1937964793776716 8 0.4472822755063163 1.800297421374085 9 0.3432577491483728 1.5543021349411408 >10 1.5399575091624238 13.286732286238689 >50 0.07749387354771585 2.648976117832495 >100 0.04026764315757882 3.730552234529255 >500 0.0040127554127152975 1.454778394274251 >1k 0.0019711780974269273 2.018831322654501 >5k 1.4079843553049478E-4 0.47026252624449655 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8225 0.28928458252102107 No Hit GTCCTACAGTGGACATTTCTAAATT 5052 0.17768580071686302 No Hit TATCAACGCAGAGTACTTTTTTTTT 4617 0.16238625136772697 No Hit GGTATCAACGCAGAGTACTTTTTTT 4551 0.16006494043199596 No Hit CTGTAGGACGTGGAATATGGCAAGA 4229 0.14873975677585385 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3854 0.13555049009556414 No Hit CTTTAGGACGTGAAATATGGCGAGG 3210 0.11290012278327995 No Hit GTCCTACAGTGTGCATTTCTCATTT 2872 0.10101219708211215 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.51713778141059E-5 7 0.0 0.0 0.0 0.0 3.51713778141059E-5 8 0.0 0.0 0.0 0.0 3.51713778141059E-5 9 0.0 0.0 0.0 0.0 3.51713778141059E-5 10 0.0 0.0 0.0 0.0 3.51713778141059E-5 11 0.0 0.0 0.0 0.0 3.51713778141059E-5 12 0.0 0.0 0.0 0.0 3.51713778141059E-5 13 0.0 0.0 0.0 0.0 3.51713778141059E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2010 0.0 14.025506 1 TAGGACC 775 0.0 12.256031 4 CGTACTG 55 0.0030733366 12.088904 5 TAGAAAT 660 0.0 11.513241 4 GTATCAA 4735 0.0 11.223728 1 TTGCGCA 60 0.0058849487 11.081495 9 GTCCTAC 2480 0.0 10.791394 1 ACACCGT 115 8.8412526E-7 10.737538 6 GTCCTAT 360 0.0 10.582309 1 ATTTCTA 1565 0.0 10.439531 15 GTCCTAA 1270 0.0 10.42399 1 TCCTACA 2650 0.0 10.394502 2 TGTAGGA 2590 0.0 10.268567 2 TAAGACT 270 0.0 10.202011 4 GTAGGAC 2465 0.0 10.018622 3 GTACGCT 95 1.647665E-4 9.9983425 3 CTTACAC 380 0.0 9.9983425 3 CCTACAG 2595 0.0 9.992562 3 GACGTGG 1170 0.0 9.985699 7 CTAATAC 230 0.0 9.9114 3 >>END_MODULE