FastQCFastQC Report
Thu 26 May 2016
SRR1512893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843221
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT104930.36905326740341327No Hit
TATCAACGCAGAGTACTTTTTTTTT72360.2545000898628703No Hit
GGTATCAACGCAGAGTACTTTTTTT62040.21820322795871303No Hit
GTCCTACAGTGGACATTTCTAAATT47300.16636061706072092No Hit
CTGTAGGACGTGGAATATGGCAAGA42130.14817701473082817No Hit
GTCCTAAAGTGTGTATTTCTCATTT36720.12914929933339686No Hit
ACGCAGAGTACTTTTTTTTTTTTTT36220.12739073044269159No Hit
CTTTAGGACGTGAAATATGGCGAGG34770.12229088065964623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.014.1657321
TTACGTC500.001488808313.3133184
CGTGCGC2100.012.21038410
GCGCCAC1950.012.17088513
GCGAATC1251.8526407E-811.39214915
GTATCAA49350.011.0034841
AATACCG700.001479436210.8682075
GTAGGAC22600.010.6042763
GGCGAGG9850.010.60071219
TAGGACC7350.010.4798734
TGTAGGA25100.010.4574142
GGACGTG21950.010.44115456
TAGGACG23350.010.3443964
GTCCTAC25300.010.3369741
ACTAGAC1855.820766E-1110.281283
TCGAACT2500.010.25185319
GTAGAAC6150.010.2060513
GTGCGCC2700.010.19703611
TATACAC7200.010.1706133
TCCTACA27050.010.1606042
Produced by FastQC (version 0.11.5)