##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512893_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2843221
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.26596173846493	31.0	30.0	34.0	26.0	34.0
2	30.286101221115068	31.0	30.0	34.0	26.0	34.0
3	30.48312530049546	31.0	31.0	34.0	27.0	34.0
4	34.08721059671408	37.0	35.0	37.0	30.0	37.0
5	33.66188804880099	37.0	35.0	37.0	28.0	37.0
6	33.53646726722967	37.0	35.0	37.0	28.0	37.0
7	33.39005304195488	37.0	35.0	37.0	27.0	37.0
8	33.33913086601429	37.0	35.0	37.0	27.0	37.0
9	34.669019397366576	39.0	35.0	39.0	27.0	39.0
10	34.60046545801399	39.0	35.0	39.0	27.0	39.0
11	34.58426763167548	39.0	35.0	39.0	27.0	39.0
12	34.433941645760214	39.0	35.0	39.0	26.0	39.0
13	34.425618339200504	39.0	35.0	39.0	26.0	39.0
14	35.51811800771027	40.0	36.0	41.0	26.0	41.0
15	35.51264428618106	40.0	36.0	41.0	27.0	41.0
16	35.50475077385824	40.0	36.0	41.0	26.0	41.0
17	35.49721882329935	40.0	36.0	41.0	26.0	41.0
18	35.42306313860231	40.0	36.0	41.0	26.0	41.0
19	35.4304375213886	40.0	36.0	41.0	25.0	41.0
20	35.39893592513561	40.0	36.0	41.0	25.0	41.0
21	35.36945844167583	40.0	36.0	41.0	25.0	41.0
22	35.3303647518079	40.0	36.0	41.0	25.0	41.0
23	35.173757509528805	40.0	35.0	41.0	25.0	41.0
24	35.128425824091764	40.0	35.0	41.0	25.0	41.0
25	35.06805731949785	40.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.654849016408555
1101	2	0.5237145611476777
1101	3	0.47304662018063226
1101	4	0.49346106618490637
1101	5	0.6366624526568074
1101	6	0.6976859575158016
1101	7	0.678245827554953
1101	8	0.49909799831542756
1101	9	0.6990979817881353
1101	10	0.41099364896412993
1101	11	0.7100194047255428
1101	12	0.7572897219322172
1101	13	0.8914156031432228
1101	14	0.9521947171062664
1101	15	0.6414142223732071
1101	16	0.8409741922896004
1101	17	0.8555847158631451
1101	18	0.6746012415793601
1101	19	0.5674823574021914
1101	20	0.6702986466353096
1101	21	0.7923026949564189
1101	22	0.6987102313053128
1101	23	0.9017615265266627
1101	24	0.9637181955433789
1101	25	0.9648838675517339
1102	1	0.7249823237706998
1102	2	0.8550974926110833
1102	3	0.5889285127966133
1102	4	0.60976413625243
1102	5	0.7726446760636776
1102	6	0.6745912515369668
1102	7	0.7453856140444728
1102	8	0.8371710730304258
1102	9	0.9349351424540941
1102	10	0.917926417765905
1102	11	1.0131151017101487
1102	12	0.9860984304808369
1102	13	1.0153827359243266
1102	14	1.0358262064854173
1102	15	1.143725999622447
1102	16	1.1020025487236182
1102	17	1.1891743437917341
1102	18	0.5338280178717696
1102	19	0.9535303630885181
1102	20	1.009832600543291
1102	21	0.981057684157868
1102	22	0.7816138440339273
1102	23	1.1209600420683685
1102	24	0.9813538266431934
1102	25	1.055236255533913
1103	1	0.6165696785072612
1103	2	0.7269047880638944
1103	3	0.4366447316817741
1103	4	0.376784563586682
1103	5	0.5120977245628282
1103	6	0.49545696936490913
1103	7	0.5382977663831241
1103	8	0.5910719420385746
1103	9	0.6470342883830682
1103	10	0.540016481099272
1103	11	0.6354384537314743
1103	12	0.7556377325192543
1103	13	0.7347889316735561
1103	14	0.8217309848684238
1103	15	0.8179209721215557
1103	16	0.7347365510737234
1103	17	0.8086915928399065
1103	18	0.8657577810687087
1103	19	0.8100616580035904
1103	20	0.7012118937190195
1103	21	0.8097400771168495
1103	22	0.6158045055157544
1103	23	0.7651851460472869
1103	24	0.7296297309195197
1103	25	0.6745514386131433
1104	1	0.6137922236103144
1104	2	0.7775805946372039
1104	3	0.6990094021292066
1104	4	0.5285188414993911
1104	5	0.5594272281896338
1104	6	0.5610538468693562
1104	7	0.5593718583572596
1104	8	0.5009099850136707
1104	9	0.538421935030442
1104	10	0.6674196831254662
1104	11	0.7035753157784299
1104	12	0.6344804392530534
1104	13	0.6264026288901903
1104	14	0.9303986018243577
1104	15	0.9199002656039212
1104	16	0.8596911268388965
1104	17	0.7939213242506398
1104	18	0.3682428078950082
1104	19	0.8214655370599075
1104	20	0.7977593459853338
1104	21	0.7666667886061731
1104	22	0.6476061601610539
1104	23	0.6624230874678645
1104	24	0.8760002784778251
1104	25	0.8086453092473747
1105	1	0.6244230585356547
1105	2	0.5588661520930494
1105	3	0.6509335702411043
1105	4	0.5156288415694448
1105	5	0.5006567143209111
1105	6	0.6338356870856003
1105	7	0.7504559373234585
1105	8	0.6402863553986435
1105	9	0.8515316969249582
1105	10	0.6245026532744689
1105	11	0.8730614770793039
1105	12	0.7813304176871583
1105	13	0.8716807488646054
1105	14	0.9874902783899913
1105	15	0.9411822504267349
1105	16	0.8455725714635847
1105	17	0.6277208344896863
1105	18	0.6002709126081811
1105	19	0.8837976758461963
1105	20	0.93310474554481
1105	21	0.7367225697168465
1105	22	0.7727947241327016
1105	23	0.9637134884934184
1105	24	0.9529002037227343
1105	25	0.8977550331138602
1106	1	0.08773107880560715
1106	2	0.36196999585180123
1106	3	-1.438589903001862
1106	4	-0.2920779402566396
1106	5	0.05490600325937578
1106	6	0.15926846495079872
1106	7	0.29014294725308787
1106	8	0.2703337686116285
1106	9	0.5049915383794783
1106	10	0.3190183800427988
1106	11	0.3627806001183487
1106	12	0.6674457761556596
1106	13	0.5457056342593489
1106	14	0.5884333798517787
1106	15	0.6511254297860276
1106	16	0.5844913469349748
1106	17	0.6845882946851489
1106	18	0.804972713194708
1106	19	0.6407872058437931
1106	20	0.6710961407803282
1106	21	0.5959465333598146
1106	22	0.703093661775668
1106	23	0.6898864293684
1106	24	0.6713213553212611
1106	25	0.6814019617122113
1107	1	0.5947300383964631
1107	2	0.662518113840374
1107	3	0.5843496531413841
1107	4	0.4139571016046375
1107	5	0.4272740228644736
1107	6	0.44342679480396896
1107	7	0.46679410253213405
1107	8	0.36683860172421845
1107	9	0.5046778321053367
1107	10	0.5133773914239583
1107	11	0.5606774747009524
1107	12	0.6678745097029264
1107	13	0.5460518963500007
1107	14	0.6813983334661273
1107	15	0.5858794425608949
1107	16	0.7535361946868591
1107	17	0.7645244302054692
1107	18	0.7203999802548893
1107	19	0.7360482789031764
1107	20	0.850565439661807
1107	21	0.736142206603283
1107	22	0.5858153162327611
1107	23	0.7880839682273617
1107	24	0.8090575399015236
1107	25	0.8671645163071418
1108	1	0.54395554995439
1108	2	0.5425780592534686
1108	3	0.5680887353282458
1108	4	0.44556912346780564
1108	5	0.6556380450992023
1108	6	0.6300428787298529
1108	7	0.5546154090058906
1108	8	0.5323242825932937
1108	9	0.7634469167469007
1108	10	0.8068483237330852
1108	11	0.7176174220025686
1108	12	0.8560533794150231
1108	13	0.7612658422087435
1108	14	0.9185000608359886
1108	15	0.8251311939116022
1108	16	0.7555946711168247
1108	17	0.826862458693455
1108	18	0.9934375759743759
1108	19	1.0088574855544081
1108	20	0.881211030303767
1108	21	0.9967785638014846
1108	22	0.9657573246017748
1108	23	0.9355961971184996
1108	24	0.9662090829575689
1108	25	1.019150579967807
1109	1	0.1944949936743896
1109	2	0.1820555777106705
1109	3	0.28676538529098394
1109	4	0.20251477913124916
1109	5	-0.10529689383841401
1109	6	0.10311800088609857
1109	7	0.2205394457619576
1109	8	0.20716200883838098
1109	9	0.283560340109382
1109	10	0.05958147135245184
1109	11	0.2867307160786936
1109	12	0.5545994646771959
1109	13	0.3564332438385236
1109	14	0.4082881328857795
1109	15	0.4342912538191257
1109	16	0.4084753601405069
1109	17	0.4724574979708507
1109	18	0.6101931371038276
1109	19	0.5504447444074927
1109	20	0.6368351081699899
1109	21	0.6646056764592956
1109	22	0.6456544572045573
1109	23	0.5843888222756846
1109	24	0.6348805759923266
1109	25	0.48704558007191423
1110	1	0.4225317408352396
1110	2	0.28754568988160045
1110	3	0.3303101016730494
1110	4	0.31869866763237553
1110	5	0.3608674598320363
1110	6	0.42703523164358614
1110	7	0.4049185562650024
1110	8	0.4000908242924339
1110	9	0.3847569839830598
1110	10	0.558162003161705
1110	11	0.6007943803039382
1110	12	0.6378378730291487
1110	13	0.6477424828001688
1110	14	0.6545146586880151
1110	15	0.6631155209330757
1110	16	0.4736666954416151
1110	17	0.5938226173878078
1110	18	0.6504124079197311
1110	19	0.663837437862405
1110	20	0.7773737228041
1110	21	0.7449141429821609
1110	22	0.737731610595624
1110	23	0.7967272475468334
1110	24	0.8605208306567675
1110	25	0.6830288984104769
1111	1	0.17002088878057364
1111	2	0.012345745743054692
1111	3	-0.028512835209877352
1111	4	0.2036994661586462
1111	5	0.27821071926768326
1111	6	0.32264796078936797
1111	7	0.2220747694717531
1111	8	0.22899926835985696
1111	9	0.2618007335510626
1111	10	0.2447348958720923
1111	11	0.2725429924369607
1111	12	0.40056450646360275
1111	13	0.29857267884131744
1111	14	0.20271821046684124
1111	15	0.2361812885951906
1111	16	0.07429655220936127
1111	17	0.24436557987075957
1111	18	0.17560089904476683
1111	19	0.030567742866416836
1111	20	0.34111393024164727
1111	21	0.39713771429833855
1111	22	0.1415491559518003
1111	23	0.24827444233699225
1111	24	0.3780688053369943
1111	25	0.2569483460992217
1112	1	0.11312109980441676
1112	2	-0.2684885934650012
1112	3	0.10814768309000655
1112	4	0.2112392936414409
1112	5	0.08270997112712308
1112	6	0.07550524857058605
1112	7	0.2215916138462859
1112	8	0.18649466787698543
1112	9	0.2287761136230131
1112	10	-0.10268364146574527
1112	11	-0.0711541481767739
1112	12	0.32422903539166015
1112	13	0.15483918044299827
1112	14	-0.008014914520721561
1112	15	-0.1683413703867771
1112	16	-0.1654934935760295
1112	17	0.017203772620256075
1112	18	0.12520339765917043
1112	19	0.02687498149507661
1112	20	0.013454265965975765
1112	21	0.017573763089721695
1112	22	0.06186982393735718
1112	23	0.3356878617650523
1112	24	0.20772764412787126
1112	25	0.09873020370861241
1113	1	-0.6898841504292612
1113	2	-0.5481562120761225
1113	3	-0.502151861752445
1113	4	-2.0566998628667363
1113	5	-0.7810662045119727
1113	6	-0.40262447686801295
1113	7	-0.42624215718461755
1113	8	-0.3034147630177344
1113	9	-0.22400937791196895
1113	10	-0.23167967586164195
1113	11	-0.47203376583433254
1113	12	-0.10269930298893826
1113	13	-0.37627641504167997
1113	14	-0.45113756109079617
1113	15	-0.503954684837943
1113	16	-0.721854187718975
1113	17	-0.8057082331679624
1113	18	-0.5836684927921567
1113	19	-0.35282889118126803
1113	20	-0.07292376445907678
1113	21	-0.24641727520067747
1113	22	-0.2605089595464065
1113	23	-0.1396330479127812
1113	24	-0.05804025136249891
1113	25	-0.20920198564640913
1114	1	-0.9935965470288544
1114	2	-2.315724978713213
1114	3	-1.505233122307157
1114	4	-1.0754178409415545
1114	5	-0.9641071067073526
1114	6	-0.6201317041628585
1114	7	-0.5226857556618754
1114	8	-0.5249563950983358
1114	9	-0.81581748965597
1114	10	-1.0101494929609913
1114	11	-1.058725525287059
1114	12	-1.1748477416070244
1114	13	-1.0956727570286802
1114	14	-1.2245754636010275
1114	15	-1.2106653354375965
1114	16	-1.5892683904267102
1114	17	-1.7019715070609678
1114	18	-1.4411948974634257
1114	19	-1.7799634476686563
1114	20	-1.2651211483214553
1114	21	-1.5882969443193744
1114	22	-1.2400764667737079
1114	23	-1.0798182383985164
1114	24	-1.1293829539414233
1114	25	-1.6735807245192404
1115	1	-0.7483641641238954
1115	2	-0.9325477997956675
1115	3	-0.43604710900676835
1115	4	-0.13589960873209606
1115	5	-0.45279611586681767
1115	6	-0.40963757696062686
1115	7	-0.24012478169868956
1115	8	-0.5968775254340457
1115	9	-1.3981721964626814
1115	10	-0.9855401905822774
1115	11	-1.288072325186107
1115	12	-1.9294011981398
1115	13	-1.9769340194127594
1115	14	-1.4782005102084739
1115	15	-1.617521900829793
1115	16	-1.4725249707748276
1115	17	-1.452350598259926
1115	18	-1.748765816079299
1115	19	-2.1717424136117245
1115	20	-2.2910726931959857
1115	21	-1.721638898082709
1115	22	-1.765524858959651
1115	23	-1.7424610856746128
1115	24	-2.1302301968059396
1115	25	-2.0939048423013134
1116	1	-1.5252432214585205
1116	2	-1.6072910026005793
1116	3	-1.6419399886881294
1116	4	-1.311939600660196
1116	5	-1.659657981982054
1116	6	-2.454171635544249
1116	7	-2.022500218518932
1116	8	-2.3466034354365206
1116	9	-3.5235760416913884
1116	10	-2.206233087990668
1116	11	-3.1845856793560685
1116	12	-5.308027357489173
1116	13	-4.223970399932103
1116	14	-3.634116743462929
1116	15	-4.846751879259447
1116	16	-4.234521480739389
1116	17	-3.9938669562351983
1116	18	-3.939788517310305
1116	19	-4.175323189552483
1116	20	-6.01685695376144
1116	21	-4.823833333921545
1116	22	-4.1233470216847365
1116	23	-4.856633951801744
1116	24	-4.671404856794865
1116	25	-4.030910302626715
1201	1	0.6068962093549004
1201	2	0.8558310838245333
1201	3	0.9063772267673968
1201	4	0.7337184446731584
1201	5	0.1307868635476055
1201	6	0.7320109689945298
1201	7	0.9534530602942084
1201	8	0.8738304912830586
1201	9	0.916779108127983
1201	10	1.0133775951117698
1201	11	0.6681637349398244
1201	12	1.0753645992726035
1201	13	1.106849142653779
1201	14	1.0650223225126112
1201	15	0.9896664877718422
1201	16	0.9350515668020805
1201	17	0.8449442470738333
1201	18	1.058148382629561
1201	19	1.2219141781828142
1201	20	1.2242113740327767
1201	21	0.955715627299675
1201	22	0.9767912130396255
1201	23	1.0538585705574661
1201	24	0.7765025248428898
1201	25	0.9479714418555787
1202	1	0.7510473271935432
1202	2	0.8734288850794982
1202	3	0.7462828809770841
1202	4	0.7113105655352072
1202	5	0.7012548626545154
1202	6	0.842821820849899
1202	7	0.9136753926394192
1202	8	0.8447039373202472
1202	9	1.117565649754198
1202	10	1.1001274485513832
1202	11	0.9929772507853158
1202	12	1.0654711405670838
1202	13	1.071778478630499
1202	14	1.202409690736168
1202	15	1.122539537667862
1202	16	1.1849033369811792
1202	17	1.1562420574514647
1202	18	1.1810743364352803
1202	19	1.240431031253756
1202	20	1.23173811006113
1202	21	1.1971362575949556
1202	22	1.0836133498561011
1202	23	1.2924975962354495
1202	24	1.3323693076736234
1202	25	1.4035638339203018
1203	1	0.7004714520406843
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2211	9	-0.17075220547778258
2211	10	-0.2082306223271857
2211	11	-0.08433412075891056
2211	12	-0.277425715292992
2211	13	-0.0661009861744759
2211	14	-0.2072332058704447
2211	15	-0.19737933730763046
2211	16	-0.19596502770714608
2211	17	-0.34103432012447854
2211	18	-0.3058706888879925
2211	19	-0.21774958772075337
2211	20	-0.22095226933928558
2211	21	-0.14126624739824933
2211	22	-0.1875460787419172
2211	23	-0.1703562067415305
2211	24	-0.15552897511286545
2211	25	-0.3763446579327123
2212	1	-0.24499428657363254
2212	2	-0.16598053710830385
2212	3	-0.06596348346887737
2212	4	-0.17682361821005088
2212	5	-0.5650106292912938
2212	6	-0.19231499723887424
2212	7	-0.5169445538779129
2212	8	-0.1721611221042636
2212	9	-0.34513354119632567
2212	10	-0.6212732903938729
2212	11	-0.3680171339034999
2212	12	-0.17668941081553413
2212	13	-0.08994783678762985
2212	14	-0.08732082026986632
2212	15	-0.2339312480696023
2212	16	-0.22531593253201265
2212	17	-0.3403100065209941
2212	18	-0.43526882803398337
2212	19	-0.1547598253345015
2212	20	-0.3218761337711129
2212	21	-0.1942557829301279
2212	22	-0.14341480135735196
2212	23	-0.41554365326871334
2212	24	-0.3674397208804123
2212	25	-0.27501164543627965
2213	1	-0.521370346290162
2213	2	-0.18214248216192885
2213	3	-0.26457860575382597
2213	4	-0.41011727724556835
2213	5	-0.2495891149014895
2213	6	-0.2218741736418295
2213	7	-0.650692349880444
2213	8	-0.6314012295753741
2213	9	-0.5079920812169831
2213	10	-0.4500807876610935
2213	11	-0.3034212774388223
2213	12	-0.5619440501071722
2213	13	-0.459538778071817
2213	14	-0.5723956045302003
2213	15	-0.8333271147382462
2213	16	-1.1961315210661283
2213	17	-0.9217950920515676
2213	18	-0.6574865084624832
2213	19	-0.5390637176673394
2213	20	-0.5143510466657588
2213	21	-0.497935348915874
2213	22	-0.6855003564312767
2213	23	-0.8626972274505817
2213	24	-0.5871023912596129
2213	25	-0.576735383367776
2214	1	-1.1900972266694723
2214	2	-0.4752349744032962
2214	3	-0.6222606063107357
2214	4	-0.7340128082856552
2214	5	-0.9849488664792574
2214	6	-1.5330730561503927
2214	7	-0.9760126793763604
2214	8	-0.8953522443175785
2214	9	-0.5979346530680942
2214	10	-0.9173586565057334
2214	11	-1.0913234101128424
2214	12	-0.7681789752376318
2214	13	-1.1430254739334984
2214	14	-0.763508925126736
2214	15	-0.937353662195548
2214	16	-0.9299954401233563
2214	17	-1.0869547646944895
2214	18	-1.1144502102638754
2214	19	-1.0193975225285854
2214	20	-1.2926814042641652
2214	21	-1.374972672860963
2214	22	-1.8975259910252689
2214	23	-1.9064684389753808
2214	24	-1.5255388402922492
2214	25	-1.9058437620155075
2215	1	-1.654518351387111
2215	2	-1.0319935681679198
2215	3	-1.1174414695719825
2215	4	-1.1554902362373198
2215	5	-1.5844308503291558
2215	6	-1.4860499469051547
2215	7	-1.8818870814552433
2215	8	-1.6454880207943035
2215	9	-1.6902523556955984
2215	10	-1.6823856062930673
2215	11	-1.411161961345151
2215	12	-1.6257694005931995
2215	13	-1.850624856928377
2215	14	-2.1735651276461496
2215	15	-1.7896430789721762
2215	16	-1.9414329806955024
2215	17	-2.0792156885434707
2215	18	-1.8147095582376735
2215	19	-2.082339554313613
2215	20	-2.097309434239264
2215	21	-1.8567482955528547
2215	22	-2.0889905373373097
2215	23	-2.5435878818008035
2215	24	-2.426977772880498
2215	25	-2.6186773242662866
2216	1	-1.3358504903056136
2216	2	-0.8281975558199335
2216	3	-0.9003078249743055
2216	4	-1.1001071869133554
2216	5	-1.917169520588022
2216	6	-2.1618817322622164
2216	7	-2.062557081410464
2216	8	-2.0924510562200602
2216	9	-3.9988348613043385
2216	10	-1.988159975062743
2216	11	-2.159696930546424
2216	12	-2.394094345747412
2216	13	-2.373962151289753
2216	14	-2.5334729696267786
2216	15	-2.3716176331326153
2216	16	-1.8117020863458464
2216	17	-2.0354026677934627
2216	18	-2.4418721960950904
2216	19	-2.7288627853563483
2216	20	-2.0237493167844036
2216	21	-2.5645613114177337
2216	22	-2.1763500338104507
2216	23	-3.3249799450079003
2216	24	-2.5218987416185
2216	25	-2.610963904034598
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77421.0
3	2.0
4	1305.0
5	11237.0
6	13239.0
7	10283.0
8	8472.0
9	7225.0
10	5981.0
11	5579.0
12	5042.0
13	4679.0
14	4522.0
15	4673.0
16	4808.0
17	4981.0
18	5633.0
19	6655.0
20	8658.0
21	11355.0
22	14435.0
23	17995.0
24	20691.0
25	23827.0
26	26590.0
27	29432.0
28	33089.0
29	40006.0
30	49984.0
31	63336.0
32	80145.0
33	103710.0
34	135094.0
35	177129.0
36	243629.0
37	379235.0
38	1203144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.269076344804425	18.815997623223137	13.33357035393277	26.581355678039664
2	13.927591597779424	19.6973359610945	39.85438937804609	26.520683063079993
3	17.85896082432738	24.406497160930776	29.02844064549469	28.70610136924715
4	12.052560657803147	15.927780097283224	36.04400080336569	35.975658441547935
5	13.401349583874788	38.340684444888474	34.095813278717706	14.162152692519033
6	33.36149894053444	35.45340234317675	17.259945092902512	13.925153623386299
7	29.52746426519785	31.24211525248088	20.55022161772491	18.680198864596356
8	26.786173025801354	36.031417135352406	19.266942892715953	17.91546694613029
9	27.42138050596788	14.607437260602444	19.1954272076641	38.77575502576558
10	16.395836610283766	28.47873140407605	32.22351599450915	22.901915991131034
11	35.51477180755514	22.10018441676571	23.146010030893237	19.239033744785907
12	24.338845416867144	24.304306965934256	30.021475741486377	21.335371875712223
13	29.518538228556373	21.235734176177804	25.332677235285367	23.91305035998046
14	21.89615022212624	21.209108178346746	26.655782228704737	30.238959370822272
15	23.998049848462248	29.79334866064268	22.744556882233923	23.46404460866115
16	23.20990398793377	27.26403599608298	26.299061265378253	23.226998750605
17	22.388047102222824	27.897152854303393	26.77351743330239	22.94128261017139
18	22.35654541233741	26.4680309244265	28.741250624327307	22.43417303890878
19	24.110239865052606	26.045325272880877	27.15837396269391	22.686060899372603
20	24.765614888097286	25.77029836971508	27.370827916870667	22.09325882531697
21	24.180812341530515	25.53371836721343	26.564066779379523	23.721402511876526
22	24.301651023527665	26.57201061055724	26.702461939092696	22.423876426822396
23	23.31148370105595	26.3812063852933	27.306459821448982	23.000850092201766
24	23.837612341777163	25.617459916059985	27.680296396235114	22.864631345927737
25	23.353881639085838	26.218240663135695	27.524647239625228	22.90323045815324
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	94.0
1	94.0
2	331.0
3	568.0
4	568.0
5	568.0
6	1483.5
7	2399.0
8	2399.0
9	2399.0
10	3122.5
11	3846.0
12	3846.0
13	3846.0
14	5819.5
15	7793.0
16	7793.0
17	7793.0
18	15588.0
19	23383.0
20	23383.0
21	23383.0
22	38615.5
23	53848.0
24	53848.0
25	53848.0
26	81641.0
27	109434.0
28	109434.0
29	109434.0
30	142201.5
31	174969.0
32	174969.0
33	174969.0
34	202766.0
35	230563.0
36	230563.0
37	230563.0
38	256406.0
39	282249.0
40	282249.0
41	282249.0
42	306403.5
43	330558.0
44	330558.0
45	330558.0
46	355769.0
47	380980.0
48	380980.0
49	380980.0
50	378463.0
51	375946.0
52	375946.0
53	375946.0
54	343496.5
55	311047.0
56	311047.0
57	311047.0
58	272809.0
59	234571.0
60	234571.0
61	234571.0
62	196568.0
63	158565.0
64	158565.0
65	158565.0
66	123958.5
67	89352.0
68	89352.0
69	89352.0
70	66877.5
71	44403.0
72	44403.0
73	44403.0
74	31610.0
75	18817.0
76	18817.0
77	18817.0
78	12871.0
79	6925.0
80	6925.0
81	6925.0
82	4603.0
83	2281.0
84	2281.0
85	2281.0
86	1403.0
87	525.0
88	525.0
89	525.0
90	309.5
91	94.0
92	94.0
93	94.0
94	52.5
95	11.0
96	11.0
97	11.0
98	5.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08437613537604007
2	0.04424559329014523
3	0.01983665708715573
4	0.006401190762167275
5	0.01994217122059805
6	0.0595803140170954
7	0.08064796932774485
8	0.05581697659098607
9	0.02352965175763685
10	0.049310271695376474
11	0.007174961074077605
12	4.5722791158337675E-4
13	0.005275706672115885
14	6.330848006539063E-4
15	0.012837552902148655
16	0.008616987564455947
17	0.004501936360205556
18	0.005662591828071051
19	0.0013716837347501304
20	0.0013013409791219185
21	2.8137102251284726E-4
22	5.275706672115886E-4
23	0.0
24	0.0
25	7.034275562821181E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2843221.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.283443176399956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.19577870068377	33.89293892117611
2	15.078497828001225	14.560835861275345
3	5.870998044272541	8.504160013781656
4	2.8882227884224223	5.578133635423098
5	1.6311695827064918	3.9379241928840467
6	1.0521698597790201	3.0481430181936613
7	0.6682924921309393	2.2587223798313247
8	0.45279351472452406	1.7489943951075182
9	0.33720565413370346	1.4653305036112905
>10	1.6902848106230928	14.072447146237154
>50	0.08365423809790638	2.7485468439544194
>100	0.044429729260842314	3.8936476633726262
>500	0.0038724284232663085	1.317497668197685
>1k	0.0024111346785857662	2.1283658710101223
>5k	1.4612937445974343E-4	0.4741382921943287
>10k+	7.306468722987171E-5	0.37017359374963477
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10493	0.36905326740341327	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7236	0.2545000898628703	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6204	0.21820322795871303	No Hit
GTCCTACAGTGGACATTTCTAAATT	4730	0.16636061706072092	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4213	0.14817701473082817	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3672	0.12914929933339686	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3622	0.12739073044269159	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3477	0.12229088065964623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	7.03427556282118E-5
13	0.0	0.0	0.0	0.0	7.03427556282118E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2155	0.0	14.165732	1
TTACGTC	50	0.0014888083	13.313318	4
CGTGCGC	210	0.0	12.210384	10
GCGCCAC	195	0.0	12.170885	13
GCGAATC	125	1.8526407E-8	11.392149	15
GTATCAA	4935	0.0	11.003484	1
AATACCG	70	0.0014794362	10.868207	5
GTAGGAC	2260	0.0	10.604276	3
GGCGAGG	985	0.0	10.600712	19
TAGGACC	735	0.0	10.479873	4
TGTAGGA	2510	0.0	10.457414	2
GGACGTG	2195	0.0	10.4411545	6
TAGGACG	2335	0.0	10.344396	4
GTCCTAC	2530	0.0	10.336974	1
ACTAGAC	185	5.820766E-11	10.28128	3
TCGAACT	250	0.0	10.251853	19
GTAGAAC	615	0.0	10.206051	3
GTGCGCC	270	0.0	10.197036	11
TATACAC	720	0.0	10.170613	3
TCCTACA	2705	0.0	10.160604	2
>>END_MODULE