FastQCFastQC Report
Thu 26 May 2016
SRR1512892_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512892_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973148
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT78240.2631554164138482No Hit
GTATCAACGCAGAGTACTTTTTTTT76550.25747120560429554No Hit
CTGTAGGACGTGGAATATGGCAAGA63020.21196388474438543No Hit
GTCCTAAAGTGTGTATTTCTCATTT58350.1962566276552664No Hit
CTTTAGGACGTGAAATATGGCGAGG50390.1694836583984383No Hit
TATCAACGCAGAGTACTTTTTTTTT42550.1431143017434719No Hit
GGTATCAACGCAGAGTACTTTTTTT41830.14069262613230152No Hit
GTCCTACAGTGTGCATTTCTCATTT41740.14038991668090522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2250.014.35382518
GGTATCA24250.013.712071
TAGGACC11950.013.0352094
TAGACCG701.0934615E-412.2119924
CGCGCCA550.003073912.08863710
GGCGAGG14250.011.93149419
CCGTGCG752.078651E-411.397869
GTCCTAC37400.010.8779141
GTCCTAA17100.010.8092351
TGTAGGA34950.010.7619272
GTATCAA52700.010.7209541
ACCGCTC803.778233E-410.6858538
TGGCGAG33800.010.56677318
TAAGACG909.5390074E-510.5535734
CATTTCT44300.010.54916314
CTGTAGG35050.010.4655491
CCAACGA2001.8189894E-1210.44838819
TCCTACA40200.010.4196842
AGGACCT22800.010.414715
CGCCCTA3200.010.38919816
Produced by FastQC (version 0.11.5)