##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512892_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2973148
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.938065982588153	31.0	30.0	34.0	23.0	34.0
2	29.129255590370878	31.0	30.0	34.0	22.0	34.0
3	29.22285099833577	31.0	30.0	34.0	23.0	34.0
4	32.6701977163599	35.0	33.0	37.0	25.0	37.0
5	32.85392822691639	35.0	35.0	37.0	26.0	37.0
6	32.755513348141434	37.0	33.0	37.0	25.0	37.0
7	32.69778766479166	37.0	33.0	37.0	25.0	37.0
8	32.60413844181319	37.0	33.0	37.0	25.0	37.0
9	33.9280536993113	39.0	34.0	39.0	23.0	39.0
10	33.79679484505985	38.0	34.0	39.0	22.0	39.0
11	33.85447478564807	38.0	34.0	39.0	23.0	39.0
12	33.75139347250793	38.0	34.0	39.0	22.0	39.0
13	33.728820428717306	38.0	34.0	39.0	22.0	39.0
14	34.5939667315586	39.0	34.0	41.0	18.0	41.0
15	34.76919245190619	39.0	34.0	41.0	22.0	41.0
16	34.7093299761734	39.0	34.0	41.0	21.0	41.0
17	34.47666345570419	39.0	34.0	41.0	18.0	41.0
18	34.4767946297998	39.0	34.0	41.0	17.0	41.0
19	34.59822652622742	39.0	34.0	41.0	18.0	41.0
20	34.56187011208322	39.0	34.0	41.0	18.0	41.0
21	34.464571222152415	39.0	34.0	41.0	18.0	41.0
22	34.47098630811517	39.0	34.0	41.0	18.0	41.0
23	34.140311548567375	39.0	34.0	41.0	10.0	41.0
24	34.20546067669689	39.0	34.0	41.0	10.0	41.0
25	33.921310005421866	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6308246788737932
1101	2	0.34483030166423134
1101	3	0.6453228297250888
1101	4	0.7398903648734603
1101	5	0.676520517771948
1101	6	0.5319165495077982
1101	7	0.29107467466791803
1101	8	0.5795420611102671
1101	9	0.6108441912546212
1101	10	0.7485737237089083
1101	11	0.6899469718304658
1101	12	0.4832510884646908
1101	13	0.8230754362392503
1101	14	0.9808315259661455
1101	15	0.7662392034633925
1101	16	0.726008867730016
1101	17	0.08881481008454983
1101	18	0.5610871340343309
1101	19	0.03376671938475084
1101	20	-0.6641788884802438
1101	21	0.4978786219173088
1101	22	0.6248247245426839
1101	23	0.23979730209594408
1101	24	0.6804824939262062
1101	25	0.2855490022796232
1102	1	0.7887421394016343
1102	2	0.9242847563200662
1102	3	0.7293475579583024
1102	4	0.9458068692177051
1102	5	0.7818958790034358
1102	6	0.6956801733489613
1102	7	0.7119780788235275
1102	8	0.7308654996790196
1102	9	1.044824731498359
1102	10	1.0625751863857928
1102	11	0.8680496574283083
1102	12	1.0856532867402464
1102	13	1.0435676341124562
1102	14	1.1997978062936099
1102	15	1.1687613507109305
1102	16	1.096407459015964
1102	17	1.2563016803110258
1102	18	1.2233131483818198
1102	19	0.9787217858560737
1102	20	1.2037109989314985
1102	21	1.382271386163616
1102	22	1.1188734283695467
1102	23	1.1030352143021958
1102	24	0.5201189074803452
1102	25	1.0221939812506875
1103	1	0.608993020168473
1103	2	0.7062054575575871
1103	3	0.7718343481852159
1103	4	0.9033207246635655
1103	5	0.7618027729899595
1103	6	0.3828952068247844
1103	7	0.273744980807173
1103	8	0.5494100517919591
1103	9	0.6864114077640266
1103	10	0.8238472724299939
1103	11	0.5841324437461353
1103	12	0.7104468905213039
1103	13	0.834920507656058
1103	14	0.9833211134664381
1103	15	0.812667421925866
1103	16	0.8896575958755335
1103	17	0.5639523492049605
1103	18	0.8704925038251403
1103	19	0.8769321192953328
1103	20	1.0492182924972369
1103	21	0.838421719077111
1103	22	0.8089977249897728
1103	23	0.14386958955152807
1103	24	0.43014246992451177
1103	25	0.7870389898081527
1104	1	0.7691950661845084
1104	2	0.6524538142739402
1104	3	0.6352509776018458
1104	4	0.8101794302357916
1104	5	0.5292504550676114
1104	6	0.43741520884378815
1104	7	0.5855518857134001
1104	8	0.3284273147396277
1104	9	0.7531283373484996
1104	10	0.7571103346876527
1104	11	0.5264188668922998
1104	12	0.7758516032609961
1104	13	0.7427864875578649
1104	14	1.0159461883265024
1104	15	0.7245979226800401
1104	16	0.6565763804890778
1104	17	0.8480062111659521
1104	18	0.7815634723063809
1104	19	0.3915277521510916
1104	20	0.4809205820216462
1104	21	0.5310853642087849
1104	22	0.35990998330600377
1104	23	0.7728241223769601
1104	24	0.5117382842178841
1104	25	0.11200253176139796
1105	1	0.776990311496121
1105	2	0.9023880705605833
1105	3	0.9093870098522601
1105	4	1.0166810778846482
1105	5	0.8070411101823041
1105	6	0.9895289477501166
1105	7	1.0388977560343022
1105	8	1.0129804592730736
1105	9	0.7255191864020603
1105	10	0.9114072936290896
1105	11	0.9342100719990825
1105	12	1.1173623443082263
1105	13	1.0754467848310725
1105	14	1.2350457730937734
1105	15	1.19351299798128
1105	16	1.1816247691726858
1105	17	1.4701990653270727
1105	18	1.3208543512818665
1105	19	1.0113289315926295
1105	20	1.1987301709642466
1105	21	1.0857893534500889
1105	22	1.060061120409003
1105	23	1.3126543280238394
1105	24	1.4478211140207335
1105	25	1.6882637253093051
1106	1	0.6148936190521574
1106	2	0.7463841277773291
1106	3	0.6157333310778377
1106	4	0.6731446485836727
1106	5	0.6118349905332252
1106	6	0.7459778073988446
1106	7	0.6811671961282215
1106	8	0.6099659229627008
1106	9	0.7680057530970359
1106	10	0.6815407566759433
1106	11	0.5579151738412236
1106	12	0.6579656747902192
1106	13	0.806824392450217
1106	14	1.0005649839974708
1106	15	0.726475684307772
1106	16	0.8556611607257736
1106	17	1.0426756604803558
1106	18	0.9569687600071433
1106	19	0.9580260466835924
1106	20	0.9989120631213453
1106	21	1.064825413727327
1106	22	1.0106926560033216
1106	23	1.220548830203093
1106	24	0.9035159953791592
1106	25	1.0602154908062928
1107	1	0.8251417379614949
1107	2	0.8314076692507726
1107	3	0.7914149944411051
1107	4	0.8419025728489729
1107	5	0.6439203656305068
1107	6	0.874023341798079
1107	7	0.4978186646229119
1107	8	0.7734184599407925
1107	9	0.7788785422247528
1107	10	0.9683571512904976
1107	11	0.8321038974736368
1107	12	0.9060844935916705
1107	13	0.6067803551735267
1107	14	0.9752297882543104
1107	15	0.657342320529807
1107	16	0.801309347067189
1107	17	1.0662395871423556
1107	18	1.0212398253796877
1107	19	0.7807733824464549
1107	20	0.9338210931894579
1107	21	0.871760886441777
1107	22	0.9346204334209602
1107	23	1.108028337115968
1107	24	0.9533806966409273
1107	25	1.2561420789277449
1108	1	0.8269337231532425
1108	2	0.7241525065225538
1108	3	0.7376427797894287
1108	4	0.9482184023433007
1108	5	0.7537669552200725
1108	6	0.872126695402244
1108	7	0.8378499574217955
1108	8	0.8532825539987314
1108	9	0.9404160517875866
1108	10	1.0716563007326272
1108	11	0.7278670883470042
1108	12	0.8683841032169681
1108	13	0.8147023028897848
1108	14	0.9045582006086406
1108	15	0.9640547898115983
1108	16	0.7096663832953283
1108	17	0.9140149937247415
1108	18	1.112512720178998
1108	19	0.9746341252861157
1108	20	0.9316699385519485
1108	21	1.0029292247411163
1108	22	0.951975655521565
1108	23	1.3375633598702308
1108	24	1.1456550863060428
1108	25	1.2954208838452672
1109	1	0.7938516043391388
1109	2	0.6595370149468103
1109	3	0.7796070882113746
1109	4	0.7865642043852219
1109	5	0.6744114738454741
1109	6	0.8309432566032271
1109	7	0.6986296367830107
1109	8	0.6841868346765878
1109	9	0.8335613498980905
1109	10	1.008506483972866
1109	11	0.8846940380534178
1109	12	0.850979017749232
1109	13	0.9487722068666784
1109	14	1.0828687352896296
1109	15	0.904049453656171
1109	16	0.632136509494174
1109	17	1.09148633851472
1109	18	1.0357635029975967
1109	19	0.9954942073800908
1109	20	0.9336540630848518
1109	21	0.8104148292413456
1109	22	0.8147383091985603
1109	23	1.1367905758476553
1109	24	0.9921420825263851
1109	25	0.6207612874070421
1110	1	0.6716608269841764
1110	2	0.7039143079644781
1110	3	0.6203035739187577
1110	4	0.5830088040151011
1110	5	0.5479485429365099
1110	6	0.5363275403396699
1110	7	0.42854283202836996
1110	8	0.5453471176941136
1110	9	0.6591870083381224
1110	10	0.607981755610723
1110	11	0.6255884223435046
1110	12	0.6031855367835632
1110	13	0.648413710227068
1110	14	0.7321780667493059
1110	15	0.6832267395395775
1110	16	0.7232482315145319
1110	17	0.7873305197270213
1110	18	0.16447123957254206
1110	19	0.6346088763992341
1110	20	0.692840968216494
1110	21	0.9549268843425409
1110	22	0.9250878417072101
1110	23	1.086602203904988
1110	24	0.879970826637404
1110	25	1.0133631268460306
1111	1	0.37339124537601975
1111	2	0.422865272751217
1111	3	0.5342088465701629
1111	4	0.4404406188809986
1111	5	0.5004143052044867
1111	6	0.33468214405530006
1111	7	0.2787368641600594
1111	8	0.5578029646103602
1111	9	0.5941845567591812
1111	10	0.6162448660196986
1111	11	0.23645755337342678
1111	12	-0.02279472701083307
1111	13	0.374595188682342
1111	14	0.3998438693779036
1111	15	0.39880533380265604
1111	16	0.4508152458098422
1111	17	0.007827199408197316
1111	18	0.3421239163454004
1111	19	0.30887122632547914
1111	20	0.515827493272532
1111	21	0.4726935625647144
1111	22	0.07340914617912375
1111	23	0.49027457915354233
1111	24	0.4271560626804103
1111	25	0.6058733238740501
1112	1	0.12164139302384314
1112	2	0.08715336954285391
1112	3	0.1255195465884711
1112	4	0.2155901794621684
1112	5	0.1789607113232492
1112	6	-0.1689376991242284
1112	7	-0.200314553387976
1112	8	-0.03507061016907187
1112	9	-0.1356773119335486
1112	10	-0.08612532210124613
1112	11	-0.10845442101536662
1112	12	-8.686600995062577E-4
1112	13	0.04567164661324341
1112	14	-0.056026883114491
1112	15	-0.2241515169273285
1112	16	-0.09864088580862784
1112	17	0.2913124359155077
1112	18	-0.14685841835566293
1112	19	0.07226217454687145
1112	20	-0.21986287527534643
1112	21	-0.1582697975644436
1112	22	-0.23740563768767942
1112	23	-0.010467700391281198
1112	24	0.06925606603311252
1112	25	0.08736151075879661
1113	1	-0.2775239824457749
1113	2	-0.08230940509322693
1113	3	-0.092079665846871
1113	4	0.013194932465566467
1113	5	-0.1309746458030645
1113	6	-0.45789165036198654
1113	7	-0.42612787644416983
1113	8	-0.34878653108751223
1113	9	-0.4539219918084356
1113	10	-0.3208255328001357
1113	11	-0.3448913900624717
1113	12	-0.4670874496164714
1113	13	-0.420093104712258
1113	14	-0.005714461492992484
1113	15	-0.34696716544907247
1113	16	-0.45788528865511324
1113	17	-0.28769138917382264
1113	18	-0.4641362516265133
1113	19	-0.8335731185220894
1113	20	-0.6450084899681627
1113	21	-0.39310236142298294
1113	22	-0.8847279440671088
1113	23	-4.483705926465685
1113	24	-2.0612071394389417
1113	25	-1.3023104858644245
1114	1	-0.43313166068442754
1114	2	-0.673137187022828
1114	3	-0.4179888014816342
1114	4	-0.3162696150255897
1114	5	-0.2672549132529838
1114	6	-0.9951593291080947
1114	7	-0.8716658531297732
1114	8	-0.5428711365416632
1114	9	-0.7489200660079547
1114	10	-0.6152216604630709
1114	11	-0.6462711924307314
1114	12	-1.6278644895237875
1114	13	-0.8462217449476412
1114	14	-2.813774965950369
1114	15	-1.6244466674914833
1114	16	-1.4581021703016717
1114	17	-1.2439981156133797
1114	18	-1.244042795439917
1114	19	-1.3037750036310811
1114	20	-0.8672784743908579
1114	21	-0.8363344234995012
1114	22	-0.9450322286363928
1114	23	-5.274963149288581
1114	24	-2.4501537158626583
1114	25	-2.392298422655898
1115	1	-1.1288287906164562
1115	2	-1.1319143682469246
1115	3	-0.7268636127769135
1115	4	-0.6747815790694283
1115	5	-0.712957055797034
1115	6	-0.9443562745125753
1115	7	-1.417951141735486
1115	8	-1.7461420915162158
1115	9	-2.838728803759068
1115	10	-3.7679229461065873
1115	11	-2.4127315248632613
1115	12	-2.533560304712317
1115	13	-1.8973557538981396
1115	14	-2.5591880844560784
1115	15	-2.1882368754087693
1115	16	-2.3250325174354174
1115	17	-2.19887080136602
1115	18	-4.657771156749966
1115	19	-2.784820134457817
1115	20	-3.1038770792992203
1115	21	-2.8930895828920242
1115	22	-2.415211626483874
1115	23	-2.8840315132671464
1115	24	-2.6349762584204655
1115	25	-2.9756350367498996
1116	1	-1.386231310493212
1116	2	-1.3568320005164303
1116	3	-1.6420337158172025
1116	4	-1.4303203699161067
1116	5	-1.3999707519374667
1116	6	-3.556362260714856
1116	7	-2.2051475000855767
1116	8	-2.563733650459181
1116	9	-3.5011214097390564
1116	10	-2.8185449507531786
1116	11	-2.278229727879708
1116	12	-2.1490496945056385
1116	13	-3.4974213503914413
1116	14	-6.5259897305311405
1116	15	-4.456056964491527
1116	16	-3.1580219131042213
1116	17	-4.27474761261394
1116	18	-2.8893439084053476
1116	19	-2.6260006310953443
1116	20	-3.276624556563064
1116	21	-3.9093203801845
1116	22	-3.6501326195291313
1116	23	-2.555832515650504
1116	24	-3.514754546451222
1116	25	-3.3470943994350506
1201	1	0.38201000600218293
1201	2	0.4399304150739525
1201	3	0.7595657220640675
1201	4	0.7133559117820667
1201	5	0.18474546008941672
1201	6	0.6272386917783379
1201	7	0.6683808027035809
1201	8	0.6517445561241502
1201	9	1.0968640966161658
1201	10	0.9878447729443707
1201	11	1.0089347900620567
1201	12	0.862231965239161
1201	13	0.9943071049264347
1201	14	1.1263093133033593
1201	15	1.0834503019734214
1201	16	1.0229488583443853
1201	17	1.2072530799996883
1201	18	0.9784769506234383
1201	19	1.1922827140662235
1201	20	1.223075520811527
1201	21	1.3597110688298315
1201	22	1.1156077404591542
1201	23	1.4588791721831527
1201	24	1.2710162039883883
1201	25	1.3437271142032898
1202	1	0.7785515776026557
1202	2	0.7682027118342845
1202	3	0.7969001282179065
1202	4	0.8773472760042438
1202	5	0.7509771722286018
1202	6	1.0123323615935504
1202	7	0.9885391660813241
1202	8	1.0727091079904767
1202	9	1.3584954790630803
1202	10	1.135309428519271
1202	11	1.0409569965143675
1202	12	1.2331567427318362
1202	13	1.155226005600042
1202	14	1.5900624391263278
1202	15	1.328991901540249
1202	16	1.0560503913668242
1202	17	1.344700827581569
1202	18	1.3817023843155951
1202	19	1.3467570689173414
1202	20	1.18553462001519
1202	21	1.230996253031016
1202	22	1.3460550331993204
1202	23	1.5368222330697918
1202	24	1.4598197469373275
1202	25	1.7476928309460433
1203	1	0.8383560274904482
1203	2	0.9000984674034207
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2211	15	-0.032863034012052594
2211	16	-0.07644963070934807
2211	17	0.3831943061865317
2211	18	-0.08302569093973489
2211	19	0.024813471675521726
2211	20	-0.04716585366816872
2211	21	0.282292389967445
2211	22	0.08477147635546345
2211	23	0.3098292796877331
2211	24	0.012358273648878537
2211	25	0.4639707718975643
2212	1	0.20358676631343897
2212	2	0.3202126493251676
2212	3	-0.07378992338594514
2212	4	0.38150403924723975
2212	5	0.05247234754148877
2212	6	-0.04734833006662598
2212	7	0.09585966611670926
2212	8	0.14413599683919642
2212	9	0.2199615743015002
2212	10	-0.02003153174071315
2212	11	-0.3260527636017656
2212	12	0.32403005154803566
2212	13	0.24187788916036368
2212	14	0.300905132497185
2212	15	0.1077153026007096
2212	16	0.027869062565272884
2212	17	0.24123494369126774
2212	18	0.4804303445641267
2212	19	0.19888818433424404
2212	20	0.3848172658383504
2212	21	0.4735741989112512
2212	22	0.4673171418416331
2212	23	0.3861891647474778
2212	24	0.5020485461027491
2212	25	0.6644859123171329
2213	1	-0.19758817409602258
2213	2	-0.08466936497205779
2213	3	-0.26594975626596096
2213	4	-0.10901289249771651
2213	5	-0.1869441624627015
2213	6	0.06237313787796239
2213	7	-0.1338157470012078
2213	8	-0.051388623220681495
2213	9	-0.4938030427858848
2213	10	-0.16955358765279982
2213	11	-0.21675483792677142
2213	12	-0.2519879980707742
2213	13	-0.30938104660570787
2213	14	-0.40101765933131617
2213	15	-0.21575877237133767
2213	16	-0.22433365748810985
2213	17	-0.25214882688955953
2213	18	-0.2668818415557723
2213	19	-0.28678719200286196
2213	20	-0.03504664141696168
2213	21	-0.2527783684079452
2213	22	-0.5201141427881524
2213	23	-0.28981028804416553
2213	24	-0.13481434367530198
2213	25	0.11661015413518783
2214	1	-2.6974294898551925
2214	2	-1.5181009627669226
2214	3	-1.6824909008088547
2214	4	-3.5424065261653865
2214	5	-1.8449600119047247
2214	6	-1.4474470777679649
2214	7	-1.3126288682945564
2214	8	-1.1423852948707847
2214	9	-1.0392956888342226
2214	10	-1.0612086173137243
2214	11	-1.115280793525983
2214	12	-1.0836710863208125
2214	13	-1.3676530764284678
2214	14	-1.3459156341291418
2214	15	-1.7123834031290386
2214	16	-1.6046421586343484
2214	17	-3.6143365271515435
2214	18	-3.400601521219887
2214	19	-2.2439716948524477
2214	20	-2.0898043745983017
2214	21	-2.072422131245659
2214	22	-3.0206522746063804
2214	23	-2.088508215328723
2214	24	-3.1285113300886955
2214	25	-2.990061537613144
2215	1	-1.7205880913625045
2215	2	-3.2228078989151463
2215	3	-1.9588304897223487
2215	4	-3.307079876214427
2215	5	-2.0856241349609412
2215	6	-1.9367784156577557
2215	7	-2.0331758460531937
2215	8	-1.455295737256943
2215	9	-3.726926404163031
2215	10	-2.088860737254965
2215	11	-1.7432522907805605
2215	12	-1.707888752086916
2215	13	-1.7803874189883082
2215	14	-1.4576850772563432
2215	15	-1.6811231944872418
2215	16	-1.7342496972344321
2215	17	-1.9934007086015555
2215	18	-1.673704218404076
2215	19	-1.725377622112532
2215	20	-1.63872029478636
2215	21	-2.1461056726552172
2215	22	-1.9862073806070697
2215	23	-2.1700779642499093
2215	24	-2.117635727721428
2215	25	-3.1402347129158557
2216	1	-2.970903460729261
2216	2	-2.9876406615801017
2216	3	-4.2234135941290205
2216	4	-4.831176586556637
2216	5	-4.4210320932574625
2216	6	-3.402242942647657
2216	7	-5.076328484826703
2216	8	-2.973940403522164
2216	9	-3.719554452916718
2216	10	-2.6064792260313325
2216	11	-2.9654821316820232
2216	12	-3.305291075491059
2216	13	-2.89508971177122
2216	14	-3.8953710206466248
2216	15	-3.4070066316250838
2216	16	-3.6015677609764047
2216	17	-3.3545114534300104
2216	18	-3.001496281205437
2216	19	-3.6010177743103036
2216	20	-4.260688023819242
2216	21	-3.8820640805270727
2216	22	-3.4527659206337056
2216	23	-3.5278212528528066
2216	24	-3.809266022520358
2216	25	-3.854750787596494
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	141861.0
3	3.0
4	1333.0
5	7836.0
6	9408.0
7	8455.0
8	7524.0
9	6877.0
10	6056.0
11	5821.0
12	5714.0
13	5471.0
14	5501.0
15	5612.0
16	5818.0
17	6230.0
18	6994.0
19	8371.0
20	11115.0
21	14388.0
22	18018.0
23	21845.0
24	25378.0
25	29058.0
26	32165.0
27	35793.0
28	39922.0
29	47745.0
30	58212.0
31	71969.0
32	90317.0
33	113592.0
34	147780.0
35	192326.0
36	261615.0
37	396726.0
38	1120299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.658967418817035	18.26216816399263	12.819922647533295	24.258941769657046
2	14.773263186929626	19.45617475604054	42.39381330247609	23.37674875455374
3	19.691026051575715	24.798074050951403	28.45453823777142	27.056361659701462
4	12.224628481816145	15.83946727699995	37.93017496624397	34.00572927493994
5	13.242990952867112	38.69609605871974	34.587429547912464	13.473483440500678
6	32.296983789941116	35.8419159080474	18.298623780946752	13.56247652106473
7	29.27215062044896	31.445001131026757	20.942879109397218	18.339969139127064
8	25.934535542014114	36.662931509930374	19.948767297266176	17.453765650789332
9	27.83650669204411	14.951809674555824	19.693585541320203	37.51809809207986
10	16.434373468211515	28.478809473791937	33.041451879622294	22.045365178374244
11	34.923107894022806	22.24710371159855	24.244022775764293	18.585765618614346
12	24.683017124818868	24.357684621061534	30.676872067529644	20.28242618658996
13	29.46533406942612	21.844350039048823	25.544899415614143	23.145416475910917
14	21.568714582779972	22.23035782396331	27.03843112382734	29.16249646942938
15	23.51078595168548	30.93755859551865	22.975603729511135	22.576051723284735
16	22.23744960907584	27.8028294661718	27.75512746063406	22.204593464118297
17	21.767428079868424	28.550554099392787	27.40592599326293	22.276091827475856
18	21.598122335713636	27.049873858319305	29.929836741386488	21.422167064580567
19	23.897017358928615	26.390581112195555	27.448896352440016	22.263505176435814
20	24.50612632958219	26.462296324875584	27.92410448258657	21.107472862955653
21	23.855210059932485	26.19397066526272	26.62218969689054	23.328629577914253
22	24.07197846504526	27.783795249582923	26.728966298603897	21.41525998676792
23	22.769221860413754	27.532535828465075	27.56350950622571	22.134732804895457
24	23.537305312940177	26.276390314057867	28.275004645826428	21.911299727175525
25	22.952473675599354	27.03167007693815	28.048961129638123	21.966895117824375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	301.0
1	301.0
2	769.5
3	1238.0
4	1238.0
5	1238.0
6	2067.5
7	2897.0
8	2897.0
9	2897.0
10	3462.5
11	4028.0
12	4028.0
13	4028.0
14	6318.5
15	8609.0
16	8609.0
17	8609.0
18	17507.5
19	26406.0
20	26406.0
21	26406.0
22	43981.5
23	61557.0
24	61557.0
25	61557.0
26	94118.5
27	126680.0
28	126680.0
29	126680.0
30	167148.0
31	207616.0
32	207616.0
33	207616.0
34	239547.5
35	271479.0
36	271479.0
37	271479.0
38	295426.0
39	319373.0
40	319373.0
41	319373.0
42	341376.0
43	363379.0
44	363379.0
45	363379.0
46	384826.5
47	406274.0
48	406274.0
49	406274.0
50	394056.0
51	381838.0
52	381838.0
53	381838.0
54	340020.0
55	298202.0
56	298202.0
57	298202.0
58	257541.5
59	216881.0
60	216881.0
61	216881.0
62	179606.5
63	142332.0
64	142332.0
65	142332.0
66	109144.5
67	75957.0
68	75957.0
69	75957.0
70	55998.0
71	36039.0
72	36039.0
73	36039.0
74	25269.0
75	14499.0
76	14499.0
77	14499.0
78	9941.5
79	5384.0
80	5384.0
81	5384.0
82	3471.0
83	1558.0
84	1558.0
85	1558.0
86	1017.5
87	477.0
88	477.0
89	477.0
90	291.0
91	105.0
92	105.0
93	105.0
94	66.0
95	27.0
96	27.0
97	27.0
98	19.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3393036606317614
2	0.010695733949335855
3	0.009283089842819798
4	0.011637496687013226
5	0.012881968876086896
6	0.08072252037234608
7	0.08058798283839216
8	0.10662099565847379
9	0.08512862460933664
10	0.0929654359621519
11	0.08818935350678808
12	0.09942323759193959
13	0.08435503378910164
14	0.08963563199679263
15	0.08647399994887574
16	0.08822298789027656
17	0.10134039745078281
18	0.10342572922706843
19	0.09622797116053422
20	0.09599253047611488
21	0.08314419598351647
22	0.104031148129861
23	0.0970015619807692
24	0.09215821075842844
25	0.09114917925377411
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2973148.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.79685687821174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.84439532610405	37.00266323759983
2	14.122284164233006	14.347352949679603
3	5.385442702767851	8.206906865949252
4	2.581705964049847	5.24570193429863
5	1.4527286550764331	3.68970247873973
6	0.8850417497113026	2.697440345479628
7	0.5603466542168766	1.9924694157507634
8	0.40776507861758376	1.6570547470776127
9	0.29436200487334335	1.3457398168741221
>10	1.3363911052293085	11.759983605937494
>50	0.07613556262530692	2.6790545474320955
>100	0.046006672853460044	4.434997452424136
>500	0.00446326212614848	1.580405415163226
>1k	0.0025980182523054197	2.255522502155685
>5k	3.330792631160795E-4	1.1050046854383604
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	7824	0.2631554164138482	No Hit
GTATCAACGCAGAGTACTTTTTTTT	7655	0.25747120560429554	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6302	0.21196388474438543	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5835	0.1962566276552664	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5039	0.1694836583984383	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4255	0.1431143017434719	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4183	0.14069262613230152	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4174	0.14038991668090522	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.726876697695507E-5
2	0.0	0.0	0.0	0.0	6.726876697695507E-5
3	0.0	0.0	0.0	0.0	6.726876697695507E-5
4	0.0	0.0	0.0	0.0	6.726876697695507E-5
5	0.0	0.0	0.0	0.0	6.726876697695507E-5
6	0.0	0.0	0.0	3.3634383488477536E-5	1.009031504654326E-4
7	0.0	0.0	0.0	3.3634383488477536E-5	1.009031504654326E-4
8	0.0	0.0	0.0	3.3634383488477536E-5	1.009031504654326E-4
9	0.0	0.0	0.0	3.3634383488477536E-5	1.009031504654326E-4
10	0.0	0.0	0.0	6.726876697695507E-5	1.009031504654326E-4
11	0.0	0.0	0.0	6.726876697695507E-5	1.009031504654326E-4
12	0.0	0.0	0.0	6.726876697695507E-5	1.3453753395391014E-4
13	0.0	0.0	0.0	6.726876697695507E-5	1.3453753395391014E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAACG	225	0.0	14.353825	18
GGTATCA	2425	0.0	13.71207	1
TAGGACC	1195	0.0	13.035209	4
TAGACCG	70	1.0934615E-4	12.211992	4
CGCGCCA	55	0.0030739	12.088637	10
GGCGAGG	1425	0.0	11.931494	19
CCGTGCG	75	2.078651E-4	11.39786	9
GTCCTAC	3740	0.0	10.877914	1
GTCCTAA	1710	0.0	10.809235	1
TGTAGGA	3495	0.0	10.761927	2
GTATCAA	5270	0.0	10.720954	1
ACCGCTC	80	3.778233E-4	10.685853	8
TGGCGAG	3380	0.0	10.566773	18
TAAGACG	90	9.5390074E-5	10.553573	4
CATTTCT	4430	0.0	10.549163	14
CTGTAGG	3505	0.0	10.465549	1
CCAACGA	200	1.8189894E-12	10.448388	19
TCCTACA	4020	0.0	10.419684	2
AGGACCT	2280	0.0	10.41471	5
CGCCCTA	320	0.0	10.389198	16
>>END_MODULE