FastQCFastQC Report
Thu 26 May 2016
SRR1512892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973148
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT99070.3332158372203469No Hit
GTCCTACAGTGGACATTTCTAAATT71490.2404522075591259No Hit
TATCAACGCAGAGTACTTTTTTTTT70770.23803053194795548No Hit
CTGTAGGACGTGGAATATGGCAAGA61010.20520337366320146No Hit
GGTATCAACGCAGAGTACTTTTTTT56570.19026970739431742No Hit
CTTTAGGACGTGAAATATGGCGAGG55840.18781439739965855No Hit
GTCCTAAAGTGTGTATTTCTCATTT53040.17839677002288484No Hit
GTCCTACAGTGTGCATTTCTCATTT39050.13134226752250477No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34940.1175185359087405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26150.013.4253341
TGGACCG905.345264E-712.6793495
TAGGACC11900.011.9068674
GCGCCAC2600.011.68393513
TCCAACG1552.5465852E-1111.63603818
GGCGAGG13700.011.57044219
ATACGGC752.0888816E-411.39183615
TAGGACA10350.011.2092784
ACCGCTA1201.276585E-711.0834128
TAGGGTA1053.4277364E-610.8680135
CTACTCG700.001479691710.8680124
GGACCGT1158.715688E-710.749526
GTAGGAC34350.010.7436293
TGTAGGA35800.010.70700552
GTACTAG1909.094947E-1210.5156511
GTCCTAC36600.010.2940091
TAGACGA1201.5314454E-610.2882779
CTGTAGG36100.010.2784561
GTATAGG3150.010.2692531
CTACACT4750.010.2102134
Produced by FastQC (version 0.11.5)