##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512892_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2973148 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.48982862608925 31.0 31.0 34.0 27.0 34.0 2 30.49014949810773 31.0 31.0 34.0 27.0 34.0 3 30.667056937629745 31.0 31.0 34.0 27.0 34.0 4 34.28033182337374 37.0 35.0 37.0 30.0 37.0 5 33.90788147781409 37.0 35.0 37.0 30.0 37.0 6 33.806639292763094 37.0 35.0 37.0 29.0 37.0 7 33.66347285772521 37.0 35.0 37.0 28.0 37.0 8 33.613110749952575 37.0 35.0 37.0 28.0 37.0 9 34.97091702128518 39.0 35.0 39.0 28.0 39.0 10 34.88944546319255 39.0 35.0 39.0 27.0 39.0 11 34.88974077307958 39.0 35.0 39.0 27.0 39.0 12 34.75639154189432 39.0 35.0 39.0 27.0 39.0 13 34.7611861232606 39.0 35.0 39.0 27.0 39.0 14 35.86834224196037 40.0 36.0 41.0 27.0 41.0 15 35.874675932715085 40.0 36.0 41.0 27.0 41.0 16 35.87382868259501 40.0 36.0 41.0 27.0 41.0 17 35.86534844548606 40.0 36.0 41.0 27.0 41.0 18 35.78818040676078 40.0 36.0 41.0 27.0 41.0 19 35.80700220776093 40.0 36.0 41.0 27.0 41.0 20 35.77766596213845 40.0 36.0 41.0 27.0 41.0 21 35.74600995308676 40.0 36.0 41.0 27.0 41.0 22 35.71682136240779 40.0 36.0 41.0 27.0 41.0 23 35.56213784177579 40.0 36.0 41.0 27.0 41.0 24 35.525297765197024 40.0 36.0 41.0 27.0 41.0 25 35.47196742308153 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5934280842133752 1101 2 0.5174530955634822 1101 3 0.4256485354705468 1101 4 0.4452587025250949 1101 5 0.5362022625061726 1101 6 0.5948811878045461 1101 7 0.5865022573702205 1101 8 0.33619755412571095 1101 9 0.643611834902245 1101 10 0.23634817184520074 1101 11 0.6376878904730887 1101 12 0.7438015577228967 1101 13 0.8319449644139709 1101 14 0.8137730927665103 1101 15 0.5371908543904595 1101 16 0.7597941016804413 1101 17 0.8966309234770335 1101 18 0.7031503494798841 1101 19 0.6152070506497012 1101 20 0.6887583642439239 1101 21 0.7278678005046189 1101 22 0.7055295403886319 1101 23 0.877078263715859 1101 24 0.8361396685801665 1101 25 0.8854012180295996 1102 1 0.7930902957902966 1102 2 0.8294264076272526 1102 3 0.5159556266133052 1102 4 0.5847580788756446 1102 5 0.706505235432445 1102 6 0.73247884752152 1102 7 0.7782700846323394 1102 8 0.716162194266964 1102 9 0.9371808848486367 1102 10 0.8361952298055471 1102 11 0.9608056643640737 1102 12 0.930401496659691 1102 13 0.9455507942937658 1102 14 0.9547597606323492 1102 15 0.9788802225282467 1102 16 1.0723641764473726 1102 17 1.0852666340942179 1102 18 0.3927594739328555 1102 19 0.738004929058178 1102 20 0.9677259699743956 1102 21 0.8382117189165967 1102 22 0.6725871220488386 1102 23 0.9718106388143752 1102 24 0.9866792732787246 1102 25 0.9505810349819726 1103 1 0.5729353435493287 1103 2 0.8324263877958735 1103 3 0.5792565548601516 1103 4 0.46875683180481076 1103 5 0.6367594783096422 1103 6 0.5920880494653673 1103 7 0.5483183855675051 1103 8 0.626893367947865 1103 9 0.8187009000127219 1103 10 0.697545903449786 1103 11 0.782216680740639 1103 12 0.9003349600807127 1103 13 0.8356948761724539 1103 14 0.9251362478127518 1103 15 0.9649904637746474 1103 16 1.0037708529805371 1103 17 0.9403860120715208 1103 18 1.0009897263770782 1103 19 0.9353425896289238 1103 20 0.8024619197890246 1103 21 0.8850003039754526 1103 22 0.8262792403674553 1103 23 0.9034904810488413 1103 24 0.8300418263795422 1103 25 0.8944790352872829 1104 1 0.5802184949069513 1104 2 0.6708935264428604 1104 3 0.5863064292862248 1104 4 0.42707024413702044 1104 5 0.5329208677488779 1104 6 0.45422324731649155 1104 7 0.42647948125060964 1104 8 0.488666872872372 1104 9 0.5494214256088412 1104 10 0.5953365480898967 1104 11 0.6792589293301745 1104 12 0.5956043949366503 1104 13 0.5265517846483121 1104 14 0.7207039406402345 1104 15 0.765788652854468 1104 16 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2216 14 -2.60971915137133 2216 15 -2.5863770146380674 2216 16 -1.8216040473572477 2216 17 -2.1745917610341507 2216 18 -2.502324843338954 2216 19 -2.7270143011883263 2216 20 -2.008599494216824 2216 21 -2.639546049935454 2216 22 -1.8812338867244947 2216 23 -3.1319774597581613 2216 24 -2.3681205007506136 2216 25 -2.45865843449004 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68932.0 3 0.0 4 1170.0 5 9739.0 6 11834.0 7 9241.0 8 7614.0 9 6380.0 10 5638.0 11 5000.0 12 4624.0 13 4228.0 14 4282.0 15 4356.0 16 4520.0 17 4694.0 18 5276.0 19 6432.0 20 8420.0 21 11110.0 22 14265.0 23 17598.0 24 20724.0 25 24399.0 26 27158.0 27 29943.0 28 33604.0 29 41166.0 30 52019.0 31 65923.0 32 84083.0 33 108534.0 34 141473.0 35 186286.0 36 255896.0 37 398262.0 38 1288325.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.73748033611318 19.225213403457342 13.752652289292175 26.2846539711373 2 13.338860739645032 20.086726889373587 41.20311266554458 25.3712997054368 3 17.968235129076955 24.799051582003067 29.218217887865855 28.01449540105412 4 11.75790735229961 16.445321684340968 36.999812644700015 34.79695831865941 5 12.924946022847692 38.86862946812741 34.67479480608258 13.531629702942318 6 32.48548221266056 36.34601049016358 17.762233055289588 13.406274241886276 7 28.961844281691356 31.456773301431994 21.06043908327179 18.520943333604865 8 25.984837063042495 36.98224035624287 19.61055455641948 17.42236802429516 9 27.218022398139734 14.791694070841208 19.672300460322848 38.31798307069621 10 15.92804271832637 28.993098788148814 32.981553694712574 22.097304798812242 11 34.824123713143415 22.25865825838513 24.171342730534263 18.745875297937197 12 24.331850275264905 24.594804946696016 30.717229215212488 20.35611556282659 13 29.293803839258388 21.786462695553606 25.689832053468415 23.229901411719595 14 21.363254913634062 22.14028767904262 27.115557640917494 29.380899766405825 15 23.367892031540137 31.094721332651577 22.98157915982669 22.55580747598159 16 22.299771936788144 27.79865180331398 27.57227047355072 22.329305786347152 17 21.636297036543457 28.611080931899668 27.506129369814335 22.24649266174254 18 21.460271202449256 26.979308990098154 30.089956817767483 21.470462989685103 19 23.825575818159926 26.417438651039294 27.57018493317018 22.1868005976306 20 24.310733022859075 26.446964534493787 28.037231348168202 21.205071094478935 21 23.463573093962864 26.12820419678642 26.94461598072336 23.46360672852736 22 23.816521426474065 27.587272768245896 27.069264923963743 21.5269408813163 23 22.449336528151306 27.394028147942855 27.875403444429946 22.281231879475897 24 23.441887184896277 26.221399002000574 28.390715833856913 21.94599797924624 25 22.735337337844378 27.021132826597544 28.243036755330298 22.000493080227784 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 109.0 1 109.0 2 340.0 3 571.0 4 571.0 5 571.0 6 1439.0 7 2307.0 8 2307.0 9 2307.0 10 3122.0 11 3937.0 12 3937.0 13 3937.0 14 6135.5 15 8334.0 16 8334.0 17 8334.0 18 17468.0 19 26602.0 20 26602.0 21 26602.0 22 45734.0 23 64866.0 24 64866.0 25 64866.0 26 98113.5 27 131361.0 28 131361.0 29 131361.0 30 170696.5 31 210032.0 32 210032.0 33 210032.0 34 241415.0 35 272798.0 36 272798.0 37 272798.0 38 296167.0 39 319536.0 40 319536.0 41 319536.0 42 342197.5 43 364859.0 44 364859.0 45 364859.0 46 385951.0 47 407043.0 48 407043.0 49 407043.0 50 394246.5 51 381450.0 52 381450.0 53 381450.0 54 338556.0 55 295662.0 56 295662.0 57 295662.0 58 255020.0 59 214378.0 60 214378.0 61 214378.0 62 177004.5 63 139631.0 64 139631.0 65 139631.0 66 106979.5 67 74328.0 68 74328.0 69 74328.0 70 54404.0 71 34480.0 72 34480.0 73 34480.0 74 24177.0 75 13874.0 76 13874.0 77 13874.0 78 9399.0 79 4924.0 80 4924.0 81 4924.0 82 3237.0 83 1550.0 84 1550.0 85 1550.0 86 981.0 87 412.0 88 412.0 89 412.0 90 249.5 91 87.0 92 87.0 93 87.0 94 52.0 95 17.0 96 17.0 97 17.0 98 8.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08748303145353006 2 0.043388354700136016 3 0.02081968337936759 4 0.006289629712345299 5 0.019978823792155653 6 0.06030644959484022 7 0.07809903846024482 8 0.05690937686250398 9 0.022501402553791468 10 0.05031703769876239 11 0.006928682998626372 12 4.0361260186173035E-4 13 0.005986920260949001 14 0.001446278490004534 15 0.014395516133068383 16 0.00968670244468153 17 0.005616942042575748 18 0.00558330765908727 19 0.002488944378147337 20 0.0020853317762856067 21 5.381501358156406E-4 22 9.081283541888934E-4 23 0.0 24 0.0 25 3.3634383488477536E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2973148.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.56443742475069 #Duplication Level Percentage of deduplicated Percentage of total 1 70.46183754340721 34.21939500209749 2 15.25130795524511 14.813423816762072 3 5.91635309648999 8.619730792116545 4 2.8260397218120623 5.48980116919207 5 1.5800814443630795 3.8367883215390237 6 0.9796119116354653 2.854458082989658 7 0.6422977758353564 2.1834981099828896 8 0.4299520517518689 1.6704303610357445 9 0.3235826413161737 1.4143148042341382 >10 1.4465721675470353 12.19020533593637 >50 0.0852934064040803 2.8810886041484367 >100 0.04915145704353607 4.523025126662298 >500 0.004584584139068296 1.5421392761039108 >1k 0.002847999237679777 2.1836346706966165 >5k 4.8624377228679113E-4 1.5780665265026577 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9907 0.3332158372203469 No Hit GTCCTACAGTGGACATTTCTAAATT 7149 0.2404522075591259 No Hit TATCAACGCAGAGTACTTTTTTTTT 7077 0.23803053194795548 No Hit CTGTAGGACGTGGAATATGGCAAGA 6101 0.20520337366320146 No Hit GGTATCAACGCAGAGTACTTTTTTT 5657 0.19026970739431742 No Hit CTTTAGGACGTGAAATATGGCGAGG 5584 0.18781439739965855 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5304 0.17839677002288484 No Hit GTCCTACAGTGTGCATTTCTCATTT 3905 0.13134226752250477 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3494 0.1175185359087405 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.3634383488477536E-5 0.0 3 0.0 0.0 0.0 3.3634383488477536E-5 0.0 4 0.0 0.0 0.0 3.3634383488477536E-5 0.0 5 0.0 0.0 0.0 3.3634383488477536E-5 0.0 6 3.3634383488477536E-5 0.0 0.0 3.3634383488477536E-5 0.0 7 3.3634383488477536E-5 0.0 0.0 3.3634383488477536E-5 0.0 8 3.3634383488477536E-5 0.0 0.0 3.3634383488477536E-5 0.0 9 3.3634383488477536E-5 0.0 0.0 3.3634383488477536E-5 0.0 10 3.3634383488477536E-5 0.0 0.0 6.726876697695507E-5 0.0 11 3.3634383488477536E-5 0.0 0.0 6.726876697695507E-5 0.0 12 3.3634383488477536E-5 0.0 0.0 6.726876697695507E-5 0.0 13 3.3634383488477536E-5 0.0 0.0 6.726876697695507E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2615 0.0 13.425334 1 TGGACCG 90 5.345264E-7 12.679349 5 TAGGACC 1190 0.0 11.906867 4 GCGCCAC 260 0.0 11.683935 13 TCCAACG 155 2.5465852E-11 11.636038 18 GGCGAGG 1370 0.0 11.570442 19 ATACGGC 75 2.0888816E-4 11.391836 15 TAGGACA 1035 0.0 11.209278 4 ACCGCTA 120 1.276585E-7 11.083412 8 TAGGGTA 105 3.4277364E-6 10.868013 5 CTACTCG 70 0.0014796917 10.868012 4 GGACCGT 115 8.715688E-7 10.74952 6 GTAGGAC 3435 0.0 10.743629 3 TGTAGGA 3580 0.0 10.7070055 2 GTACTAG 190 9.094947E-12 10.515651 1 GTCCTAC 3660 0.0 10.294009 1 TAGACGA 120 1.5314454E-6 10.288277 9 CTGTAGG 3610 0.0 10.278456 1 GTATAGG 315 0.0 10.269253 1 CTACACT 475 0.0 10.210213 4 >>END_MODULE