FastQCFastQC Report
Thu 26 May 2016
SRR1512891_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512891_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042229
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79770.39060262095974546No Hit
CTGTAGGACGTGGAATATGGCAAGA69800.3417834141029238No Hit
GTCCTAAAGTGTGTATTTCTCATTT68880.3372785324270687No Hit
GTATCAACGCAGAGTACTTTTTTTT67760.33179432864776676No Hit
CTTTAGGACGTGAAATATGGCGAGG58540.28664757967887045No Hit
GTCCTACAGTGTGCATTTCTCATTT46140.22592960926517053No Hit
TATCAACGCAGAGTACTTTTTTTTT38690.18944986091177826No Hit
GGTATCAACGCAGAGTACTTTTTTT38260.18734431838936771No Hit
CTGTAGGACCTGGAATATGGCGAGA31100.1522845870859732No Hit
CTGAAGGACCTGGAATATGGCGAGA30300.14836729867218612No Hit
GTCCTTCAGTGTGCATTTCTCATTT27210.13323677217393348No Hit
ATTTAGAAATGTCCACTGTAGGACG24680.12084834756533179No Hit
GTACTGGTTCACTATCGGTCAGTCA24560.12026075430326373No Hit
TTTCTAAATTTTCCACCTTTTTCAG24070.11786141514981915No Hit
GAATATGGCAAGAAAACTGAAAATC20660.10116397328605166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12900.013.3269994
GGTATCA20150.011.9125671
CCGGGAC658.0377364E-411.6901513
GGACCTG21550.011.503966
AAATGTC9100.011.481687
CATTTCG1001.93294E-611.39873614
ACCTTTT8550.011.33207615
AGGACCT22900.011.2817615
GCGCCAC855.3305394E-511.17523113
GGACTCG600.005883727711.081566
AATGTCC9450.011.0564338
CCTATAC1303.2763637E-810.95951753
CACCTTT8900.010.88643314
TGTAGGA39450.010.858582
TTGCGAG700.001493129410.85593918
GGACCGG803.7773434E-410.6857916
GTAAGAC2050.010.6565713
GTAGGAC39550.010.6389983
CCAACGA1701.4006218E-1010.6159519
CGAAATC2150.010.60347413
Produced by FastQC (version 0.11.5)