##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512891_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2042229
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.079187495623653	31.0	30.0	34.0	23.0	34.0
2	29.229325408658873	31.0	30.0	34.0	23.0	34.0
3	29.336424073891813	31.0	30.0	34.0	23.0	34.0
4	32.783784286678916	35.0	33.0	37.0	26.0	37.0
5	32.97295553045227	35.0	35.0	37.0	26.0	37.0
6	32.95576891719783	37.0	35.0	37.0	26.0	37.0
7	32.94655300654334	37.0	35.0	37.0	26.0	37.0
8	32.880858610860976	37.0	35.0	37.0	26.0	37.0
9	34.21868948095439	39.0	35.0	39.0	25.0	39.0
10	34.07005433768691	39.0	34.0	39.0	25.0	39.0
11	34.146538414644	39.0	35.0	39.0	25.0	39.0
12	34.040003838942646	39.0	35.0	39.0	25.0	39.0
13	34.02053981213664	39.0	35.0	39.0	25.0	39.0
14	34.8915356700938	39.0	34.0	41.0	22.0	41.0
15	35.07753488957409	39.0	35.0	41.0	25.0	41.0
16	35.032809249109675	39.0	35.0	41.0	24.0	41.0
17	34.80528383447694	39.0	34.0	41.0	22.0	41.0
18	34.821313378666154	39.0	34.0	41.0	22.0	41.0
19	34.932410126386415	40.0	35.0	41.0	22.0	41.0
20	34.913810840997755	40.0	35.0	41.0	22.0	41.0
21	34.8028732331193	40.0	35.0	41.0	19.0	41.0
22	34.83213194994293	40.0	35.0	41.0	22.0	41.0
23	34.511193896472925	39.0	34.0	41.0	18.0	41.0
24	34.58669963064867	39.0	34.0	41.0	18.0	41.0
25	34.310032812187075	39.0	34.0	41.0	16.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.92389933511107
1101	2	0.6032830540387515
1101	3	0.8844095801184224
1101	4	1.1420413205942594
1101	5	0.9477795204515687
1101	6	0.971844341033389
1101	7	0.800219828406
1101	8	1.0311006563282135
1101	9	1.0888294145127801
1101	10	1.0877241259664174
1101	11	1.1011401120197988
1101	12	0.9201342110631572
1101	13	1.3560975521513825
1101	14	1.372745632105989
1101	15	1.2511649092331183
1101	16	1.1717288520568871
1101	17	0.620733395705912
1101	18	1.0453543577454383
1101	19	0.4166322722795144
1101	20	0.0029811357851627918
1101	21	0.9329307250057752
1101	22	0.8904970752382582
1101	23	0.5671441420075993
1101	24	1.118286107636571
1101	25	0.7217893976504115
1102	1	0.8396564870886927
1102	2	0.9138797868795798
1102	3	0.9279421453045309
1102	4	1.0435021177243513
1102	5	0.9793287862528004
1102	6	0.8745490705097723
1102	7	0.8426224672653291
1102	8	0.9341347365276462
1102	9	1.2249766697545184
1102	10	1.2343054654611478
1102	11	1.0010136920114832
1102	12	1.1249726265314877
1102	13	1.1662179931773338
1102	14	1.3314602522379317
1102	15	1.2519616846361927
1102	16	1.309771140689726
1102	17	1.3869779867536494
1102	18	1.3219937643061002
1102	19	1.1873801010982135
1102	20	1.393939445050563
1102	21	1.5468379393066911
1102	22	1.2742147355307196
1102	23	1.2886983734043511
1102	24	0.6199417283866779
1102	25	0.9898555515433358
1103	1	0.4048767188961513
1103	2	0.5508687436969453
1103	3	0.6487545756021831
1103	4	0.7620512538140005
1103	5	0.5542907835217576
1103	6	0.22147903874574126
1103	7	0.10147474184687155
1103	8	0.3603950224161494
1103	9	0.5510284467119249
1103	10	0.6660839687363165
1103	11	0.4943977201905554
1103	12	0.5576786540897558
1103	13	0.7003808841085757
1103	14	0.8013221541780027
1103	15	0.7784721935108578
1103	16	0.7592434578932128
1103	17	0.4125686393700434
1103	18	0.7466133996025732
1103	19	0.7259741902166112
1103	20	0.8528103069242832
1103	21	0.6429526431430403
1103	22	0.6961471882067372
1103	23	0.14316079578860297
1103	24	0.18733293138321727
1103	25	0.5145910660309951
1104	1	0.9583695710335647
1104	2	0.8947324465401856
1104	3	0.8761829594393831
1104	4	0.9987639074232249
1104	5	0.813851104218827
1104	6	0.6682943294868124
1104	7	0.9233242665101713
1104	8	0.6496882749288702
1104	9	0.9410522533797732
1104	10	0.8318831756216412
1104	11	0.6930368409882774
1104	12	1.0997013760946786
1104	13	1.0570125247003404
1104	14	1.2776981173499706
1104	15	1.0553360145005897
1104	16	1.0899006079052853
1104	17	1.1510710770056818
1104	18	0.9087981656800395
1104	19	0.6159046521393208
1104	20	0.74957582883264
1104	21	0.9581115591895824
1104	22	0.6968732028911759
1104	23	1.1458135301883843
1104	24	0.9341639449267731
1104	25	0.5269633783862631
1105	1	0.5520683027428319
1105	2	0.5844008686634581
1105	3	0.6743690495812622
1105	4	0.7318361678331442
1105	5	0.5201740062789071
1105	6	0.7929535682658013
1105	7	0.7685867498849035
1105	8	0.7383363385108197
1105	9	0.5641396437297814
1105	10	0.5787034958660016
1105	11	0.5962080619498806
1105	12	0.8378176021751003
1105	13	0.7496465638072678
1105	14	0.9018586835578688
1105	15	0.9436789548517552
1105	16	0.7820360233641637
1105	17	1.177540889091297
1105	18	0.9714282703958972
1105	19	0.762659493783751
1105	20	0.8856588915481609
1105	21	0.7566153555687549
1105	22	0.8443911461508193
1105	23	1.013976009091543
1105	24	1.0543730459237963
1105	25	1.2708440108594417
1106	1	0.7247570718537091
1106	2	0.8442391971047591
1106	3	0.6183513944386121
1106	4	0.8032906694592086
1106	5	0.7050420354559819
1106	6	0.7881493918282132
1106	7	0.723298445256745
1106	8	0.7548463877762117
1106	9	0.8695570123214367
1106	10	0.669579665836018
1106	11	0.5280623130198379
1106	12	0.6055164493571965
1106	13	0.7303715522973349
1106	14	0.9992476465353946
1106	15	0.7561163666432265
1106	16	0.7480145134968552
1106	17	0.9596784436822929
1106	18	0.8828499224776962
1106	19	0.9519322851134859
1106	20	0.9978302486057942
1106	21	1.0433915398871392
1106	22	0.9868225974674445
1106	23	1.2057019061796979
1106	24	0.7881955526807687
1106	25	1.080530838527352
1107	1	0.6992416550147915
1107	2	0.6713035082960062
1107	3	0.6539361103595702
1107	4	0.6866015265484577
1107	5	0.6753465907482692
1107	6	0.6918855175180454
1107	7	0.47841459485326254
1107	8	0.5807211914550336
1107	9	0.6472280655701823
1107	10	0.7883859165333931
1107	11	0.7770801608764941
1107	12	0.7544347491567862
1107	13	0.5369878710119309
1107	14	0.9351808826221912
1107	15	0.6604567143709659
1107	16	0.6608183397314562
1107	17	0.9968127531517723
1107	18	0.8935983158559608
1107	19	0.7942868515783417
1107	20	0.9527376298742709
1107	21	0.6290400137603598
1107	22	0.6654006538360591
1107	23	0.8993051059345163
1107	24	0.8163839382746474
1107	25	1.1625386852565498
1108	1	0.610424685600794
1108	2	0.5090637338927948
1108	3	0.5200875136694094
1108	4	0.661999229819024
1108	5	0.4284745783863997
1108	6	0.5708720921376695
1108	7	0.5505383099372665
1108	8	0.4953595065938785
1108	9	0.6250986994497225
1108	10	0.6573651540696162
1108	11	0.39355497022349795
1108	12	0.4598291729734001
1108	13	0.5089542834746013
1108	14	0.5418865321764059
1108	15	0.5726208019416532
1108	16	0.43349826502660704
1108	17	0.6104755697024515
1108	18	0.8207024468562523
1108	19	0.5427925693155586
1108	20	0.6455768402855142
1108	21	0.6060886534354566
1108	22	0.6868889515586005
1108	23	1.0043240600666223
1108	24	0.9125838478580661
1108	25	1.0153397465397305
1109	1	0.751321524290212
1109	2	0.7261709253509743
1109	3	0.8590061031925202
1109	4	0.9217285968091957
1109	5	0.7691968271938023
1109	6	0.8269728308734443
1109	7	0.8328097729032748
1109	8	0.7233385632851252
1109	9	0.9181732384875687
1109	10	0.9077578878406101
1109	11	0.8642849951969538
1109	12	0.8862311259874147
1109	13	0.9272237874449303
1109	14	1.0500530019465657
1109	15	0.8936947727212115
1109	16	0.6119167872559714
1109	17	1.0033231792791
1109	18	1.1246931528424042
1109	19	1.0120524128949882
1109	20	0.9536814371959892
1109	21	0.8922044584929552
1109	22	0.9537058452793445
1109	23	1.176056761835703
1109	24	1.0349485500458613
1109	25	0.6606000237899536
1110	1	0.5122012278152539
1110	2	0.5689155782790074
1110	3	0.49138604965752464
1110	4	0.5967862164629949
1110	5	0.4604214491636469
1110	6	0.5919279015249614
1110	7	0.4306526462045994
1110	8	0.3740909264838379
1110	9	0.5598522773574004
1110	10	0.608461203253249
1110	11	0.4856506311938773
1110	12	0.5230200245788907
1110	13	0.5107251331390188
1110	14	0.5573493644906833
1110	15	0.4490188362387997
1110	16	0.5769872942695145
1110	17	0.7517042832981318
1110	18	0.12568884072845066
1110	19	0.48166671356664637
1110	20	0.5835165664458657
1110	21	0.6288248849300047
1110	22	0.7562974676134573
1110	23	0.7666866923017608
1110	24	0.7370745046103622
1110	25	0.886847880353848
1111	1	0.27786957979616744
1111	2	0.20856692988132863
1111	3	0.3305241012572715
1111	4	0.18190536848715055
1111	5	0.2209524323708294
1111	6	0.18739522324356273
1111	7	0.12924054601823798
1111	8	0.3988470411275955
1111	9	0.3937473666151021
1111	10	0.4137481505218119
1111	11	0.1588521082768679
1111	12	-0.17741483315933948
1111	13	0.2761011508133038
1111	14	0.19250388013549014
1111	15	0.18416274605073824
1111	16	0.31239937084604463
1111	17	-0.038596464101701145
1111	18	0.19349608524662898
1111	19	0.2618020350707084
1111	20	0.33787897045075965
1111	21	0.28198319692386065
1111	22	-0.06341703749431105
1111	23	0.36671740367057737
1111	24	0.39767009881168036
1111	25	0.5260542703263269
1112	1	0.3064889452222097
1112	2	0.4057843346086365
1112	3	0.3507681158612108
1112	4	0.5559657808687959
1112	5	0.3769381959650886
1112	6	0.20262507173251976
1112	7	0.07756406544444872
1112	8	0.15458471968565135
1112	9	-0.012071054433903328
1112	10	0.1530530438153832
1112	11	0.12329049658783475
1112	12	0.22975555945360782
1112	13	0.2499380110123326
1112	14	0.32264000990785746
1112	15	0.08222607480981026
1112	16	0.2525261606762115
1112	17	0.48207209750585633
1112	18	0.27058575374529426
1112	19	0.33761287114943883
1112	20	0.12154036561668846
1112	21	0.3028655251376051
1112	22	0.03394025962840885
1112	23	0.23011323758775148
1112	24	0.27091564373275645
1112	25	0.2588768853906913
1113	1	0.02147216487211523
1113	2	0.08570084579741177
1113	3	0.09402838397141267
1113	4	0.11349638529926409
1113	5	0.048863581594048355
1113	6	-0.1840142783761607
1113	7	-0.2474508566789595
1113	8	-0.061791888431237396
1113	9	-0.12344499721553603
1113	10	0.007167702033058276
1113	11	-0.08764169503099595
1113	12	-0.3240538991079376
1113	13	-0.20995723380434583
1113	14	0.22729777710771515
1113	15	-0.16922465590499058
1113	16	-0.2430238556536608
1113	17	-0.044537896375139496
1113	18	-0.29122562310683264
1113	19	-0.662807200701991
1113	20	-0.37451409356426524
1113	21	-0.09033148818531345
1113	22	-0.45606707942851443
1113	23	-4.205788358985604
1113	24	-1.8450222164159769
1113	25	-1.1207750631195381
1114	1	-0.5023203838355457
1114	2	-0.7715222932593448
1114	3	-0.6294233199556736
1114	4	-0.6023212859937956
1114	5	-0.49851077579728553
1114	6	-1.2525004328555234
1114	7	-1.0900858391271875
1114	8	-0.8584804008243339
1114	9	-0.9892850287177026
1114	10	-0.6638600958623115
1114	11	-0.9280719212846549
1114	12	-1.8269711148859358
1114	13	-1.0563781162608805
1114	14	-3.0904781933847225
1114	15	-1.8176897165941597
1114	16	-1.808781075230911
1114	17	-1.544495352614426
1114	18	-1.5593336328107696
1114	19	-1.6099061119774731
1114	20	-1.2182652066277768
1114	21	-1.027760103712403
1114	22	-1.232928121612133
1114	23	-5.361211105452494
1114	24	-2.580960711989448
1114	25	-2.468859109802903
1115	1	-1.228709490639833
1115	2	-1.2287633816600056
1115	3	-0.9712736831941662
1115	4	-0.975518522810237
1115	5	-0.8703231948732864
1115	6	-1.0920521022667025
1115	7	-1.5872850682198951
1115	8	-1.860663399903956
1115	9	-3.1661439285373945
1115	10	-3.8234589229805316
1115	11	-2.5096039700005974
1115	12	-2.8455868739084877
1115	13	-2.1230183886885285
1115	14	-2.892923570751467
1115	15	-2.4921315395367145
1115	16	-2.582573844284738
1115	17	-2.4648986370353256
1115	18	-4.7784479496814285
1115	19	-2.9624967798543658
1115	20	-3.158750596683305
1115	21	-2.7656846660445993
1115	22	-2.376293123144464
1115	23	-2.804232053191413
1115	24	-2.7117970525552906
1115	25	-2.81686539163179
1116	1	-1.491715656230351
1116	2	-1.4765213601587348
1116	3	-1.760849364283441
1116	4	-1.6694345863627973
1116	5	-1.8584470426734683
1116	6	-4.2067101280060655
1116	7	-2.893064547182746
1116	8	-2.885555687145075
1116	9	-4.08452119726547
1116	10	-3.261479826784324
1116	11	-2.8659885820704325
1116	12	-2.7654993878343284
1116	13	-4.167044789200492
1116	14	-7.4390425514507115
1116	15	-5.193910863006266
1116	16	-4.0608336613310065
1116	17	-4.950317273107981
1116	18	-3.631345162484152
1116	19	-3.3470766059248653
1116	20	-4.3053587785839476
1116	21	-5.032719494233042
1116	22	-4.427815043932686
1116	23	-3.634501375016633
1116	24	-4.658555162942072
1116	25	-4.319866623223085
1201	1	0.5649521546920546
1201	2	0.6269406922481764
1201	3	0.9437028341421403
1201	4	0.9137789880012193
1201	5	0.3953779715327528
1201	6	0.9016731937877367
1201	7	0.9647238585974875
1201	8	0.9855506917897543
1201	9	1.2892848494473128
1201	10	1.2329715025887253
1201	11	1.4369123971409579
1201	12	1.264228958095515
1201	13	1.3318419857244024
1201	14	1.6073056242371493
1201	15	1.5926679868809543
1201	16	1.4866849522189227
1201	17	1.7372853947533642
1201	18	1.4369455715656017
1201	19	1.6379181285593205
1201	20	1.5915709311435862
1201	21	1.6112336960101459
1201	22	1.472134337296481
1201	23	1.812193762284359
1201	24	1.66407356098005
1201	25	1.7373005874499086
1202	1	0.6875360132142347
1202	2	0.6163992604696666
1202	3	0.6101796219611408
1202	4	0.9593398558855455
1202	5	0.8041705495911486
1202	6	1.002732493112326
1202	7	1.0457707831699068
1202	8	1.1259089220375955
1202	9	1.2910945285830877
1202	10	1.1275840352773017
1202	11	1.1682036610237816
1202	12	1.2384222297305243
1202	13	1.2805273073727506
1202	14	1.7598653087751046
1202	15	1.5041039994208774
1202	16	1.2301185137705843
1202	17	1.607511813779503
1202	18	1.598640407695342
1202	19	1.4973465921580953
1202	20	1.2662822564594336
1202	21	1.4700854627820092
1202	22	1.3613445760774212
1202	23	1.544092638479711
1202	24	1.573588427477567
1202	25	1.8556094440626083
1203	1	0.905347297211577
1203	2	0.9915627832708154
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2211	15	-0.05536914243148061
2211	16	-0.12818184989484394
2211	17	0.2682182495728611
2211	18	-0.14615295384061255
2211	19	-0.08764021997473748
2211	20	-0.14324811845279584
2211	21	0.0389635235375394
2211	22	-0.013692532639701938
2211	23	0.23206184966256416
2211	24	-0.03956176006418133
2211	25	0.3723404800808936
2212	1	0.06903940554196453
2212	2	0.192298505310859
2212	3	-0.20982883637348593
2212	4	0.12303305749398419
2212	5	-0.10335377067598728
2212	6	-0.10998953306150128
2212	7	-0.06888043010440015
2212	8	0.11519649642106344
2212	9	0.1612321104069423
2212	10	-0.027570193399434118
2212	11	-0.40582301727496883
2212	12	0.101346209374924
2212	13	0.022493013711148535
2212	14	0.14607669028401915
2212	15	-0.18099177312882375
2212	16	-0.061579256728514054
2212	17	0.09566217345291506
2212	18	0.28394395929650074
2212	19	-0.006033880004210346
2212	20	-0.004067723999320094
2212	21	0.12272978592718431
2212	22	0.2747980090411346
2212	23	0.2471081022286512
2212	24	0.26617037807010036
2212	25	0.32411533178301966
2213	1	-0.11552121808901106
2213	2	0.026376700581312917
2213	3	-0.22036566866593432
2213	4	0.0653596697023815
2213	5	0.09414146733054451
2213	6	0.13944083238948224
2213	7	-0.13653324080604534
2213	8	0.08508096277508059
2213	9	-0.21553918039988673
2213	10	0.027007607560875613
2213	11	-0.047055030756034455
2213	12	-0.17862727947805013
2213	13	-0.11370747048307095
2213	14	-0.20687813248599696
2213	15	-0.16723157190646276
2213	16	-0.08237630266253859
2213	17	-0.09176651307518569
2213	18	-0.12531134564960666
2213	19	-0.15203458168996065
2213	20	0.09629594559983445
2213	21	-0.11944047998377272
2213	22	-0.30311656770419404
2213	23	-0.04820641831971528
2213	24	0.07534784945544715
2213	25	0.3103501822789312
2214	1	-2.636725316730967
2214	2	-1.307965521963819
2214	3	-1.4787361117561382
2214	4	-3.3918623644852595
2214	5	-1.7194981477557754
2214	6	-1.529459212561612
2214	7	-1.1887281950604773
2214	8	-1.1054688523111231
2214	9	-0.922573229229414
2214	10	-0.8812490401507205
2214	11	-0.9457972270747703
2214	12	-1.008177868951698
2214	13	-1.288012869753338
2214	14	-1.202771398150503
2214	15	-1.6471096073605267
2214	16	-1.4246531907709326
2214	17	-3.372978432380588
2214	18	-3.0908174353119797
2214	19	-2.108630194912081
2214	20	-1.788718151086961
2214	21	-1.8605570273616934
2214	22	-2.871169039394161
2214	23	-1.711891604719085
2214	24	-2.689005637444051
2214	25	-2.537858655425172
2215	1	-1.6315599659616673
2215	2	-3.2260456731150846
2215	3	-1.7563307111136552
2215	4	-3.131262859808796
2215	5	-1.851889031124184
2215	6	-1.7123151158353878
2215	7	-1.9410607126116233
2215	8	-1.4032208300666724
2215	9	-3.689397057767998
2215	10	-2.1665681084228083
2215	11	-1.7186107092738183
2215	12	-1.5775823078573552
2215	13	-1.849423485266911
2215	14	-1.5132051901783754
2215	15	-1.6275526879577669
2215	16	-1.6668805548201036
2215	17	-2.0932335081637277
2215	18	-1.6605218029845474
2215	19	-1.8170814898685705
2215	20	-1.7141841483671527
2215	21	-2.0790274668355266
2215	22	-2.1198107891176363
2215	23	-1.985428143801748
2215	24	-1.868994574563466
2215	25	-2.732139519067651
2216	1	-2.881173801586609
2216	2	-2.9617374393088127
2216	3	-4.079040303558131
2216	4	-4.826496447859274
2216	5	-4.313749101761797
2216	6	-3.520695521323276
2216	7	-5.219768883640995
2216	8	-3.1097076437611655
2216	9	-3.818498861525889
2216	10	-2.7637177418859267
2216	11	-3.1943772619469257
2216	12	-3.3610028739546607
2216	13	-2.9972513355436163
2216	14	-3.8914570923990794
2216	15	-3.4416312820348836
2216	16	-3.6232399257730243
2216	17	-3.3960040536380838
2216	18	-3.044545314798583
2216	19	-3.6190243494865406
2216	20	-4.200405716059034
2216	21	-3.9701231403280524
2216	22	-3.6029188594941957
2216	23	-3.6471308642927447
2216	24	-3.9611889215151983
2216	25	-4.088303353182411
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	88215.0
3	2.0
4	816.0
5	5031.0
6	6061.0
7	5559.0
8	4808.0
9	4670.0
10	4008.0
11	3858.0
12	3714.0
13	3668.0
14	3547.0
15	3641.0
16	3667.0
17	4093.0
18	4515.0
19	5585.0
20	7291.0
21	9345.0
22	11873.0
23	14347.0
24	16862.0
25	19103.0
26	21142.0
27	23647.0
28	26650.0
29	30905.0
30	38293.0
31	47078.0
32	60013.0
33	76179.0
34	99626.0
35	132163.0
36	180753.0
37	286118.0
38	785383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87198399456004	19.03495687139244	12.711540464637295	24.381518669410234
2	14.104290644967934	20.05524910578916	43.710602813678975	22.12985743556393
3	19.4197687811452	26.04831032375341	28.770626351584294	25.7612945435171
4	11.831033231456638	16.675669229989456	38.86732245810625	32.62597508044765
5	12.296185438623382	39.618098218846214	35.460489296965555	12.625227045564843
6	30.962572950965484	36.8617694060962	19.114145320234464	13.061512322703855
7	28.116316883580204	31.80043301032344	22.161493836610642	17.92175626948571
8	24.756373789098937	38.04436322889958	20.794093268363074	16.40516971363841
9	27.89063338483999	14.619950963311675	20.300630282678913	37.188785369169416
10	15.95595302217689	28.905878207204882	34.20320952064016	20.934959249978068
11	34.026034236280225	22.123317848316095	25.716514895854726	18.134133019548955
12	24.718756371849366	24.534217811377573	31.917720799284783	18.829305017488277
13	29.85053268467292	22.55280902400524	25.766902734753465	21.829755556568372
14	20.45486289960151	23.58369939018025	27.78063936241172	28.180798347806522
15	22.538435413655762	33.32612922022582	23.188618873502147	20.946816492616275
16	20.90294389644683	28.03344737630112	29.981195407503904	21.082413319748156
17	20.743372305212898	29.720824884361203	28.50436895797399	21.031433852451904
18	20.568795971245027	27.08474051214283	32.242637003255666	20.103826513356477
19	23.49515457712584	26.573855694033554	28.088062595452772	21.842927133387835
20	24.074863720620655	27.265237264825558	28.96205154747306	19.697847467080727
21	22.747238153205007	26.704214943547722	27.352219205095825	23.19632769815145
22	23.362793773163272	29.35175431845999	27.354731569497538	19.9307203388792
23	21.281379179193504	28.955450363331416	28.915946097752965	20.847224359722116
24	23.23614704537446	26.7479490309879	29.678191824354027	20.337712099283614
25	21.985360605964072	28.053970206603168	29.287315874210012	20.67335331322275
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	321.0
1	321.0
2	712.5
3	1104.0
4	1104.0
5	1104.0
6	1899.5
7	2695.0
8	2695.0
9	2695.0
10	3114.5
11	3534.0
12	3534.0
13	3534.0
14	5130.0
15	6726.0
16	6726.0
17	6726.0
18	13505.0
19	20284.0
20	20284.0
21	20284.0
22	35411.5
23	50539.0
24	50539.0
25	50539.0
26	78335.0
27	106131.0
28	106131.0
29	106131.0
30	141171.5
31	176212.0
32	176212.0
33	176212.0
34	197372.5
35	218533.0
36	218533.0
37	218533.0
38	229993.0
39	241453.0
40	241453.0
41	241453.0
42	250331.5
43	259210.0
44	259210.0
45	259210.0
46	270900.5
47	282591.0
48	282591.0
49	282591.0
50	263502.0
51	244413.0
52	244413.0
53	244413.0
54	208255.5
55	172098.0
56	172098.0
57	172098.0
58	144142.0
59	116186.0
60	116186.0
61	116186.0
62	94855.0
63	73524.0
64	73524.0
65	73524.0
66	55690.0
67	37856.0
68	37856.0
69	37856.0
70	27707.5
71	17559.0
72	17559.0
73	17559.0
74	12408.0
75	7257.0
76	7257.0
77	7257.0
78	4980.5
79	2704.0
80	2704.0
81	2704.0
82	1813.5
83	923.0
84	923.0
85	923.0
86	592.5
87	262.0
88	262.0
89	262.0
90	174.5
91	87.0
92	87.0
93	87.0
94	54.5
95	22.0
96	22.0
97	22.0
98	13.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3388944138977558
2	0.010038051560329424
3	0.008862865036193296
4	0.010674610927569828
5	0.011653933031016599
6	0.08059820911366943
7	0.07986371753608434
8	0.1036122785446686
9	0.08402583647573313
10	0.09117488783089457
11	0.08627827731366071
12	0.09817704087053901
13	0.08402583647573313
14	0.0876493282584862
15	0.0846134297378012
16	0.08667000615503942
17	0.09989085455157085
18	0.1025839903360495
19	0.0956308034015774
20	0.0947494135084753
21	0.08108787016539282
22	0.10243709202053246
23	0.095141142349854
24	0.09068522677917118
25	0.09029349793779248
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2042229.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.238189295368876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.7109120572161	43.140827932941136
2	13.656200690583473	15.35999998985161
3	4.50584478796103	7.60201656362711
4	1.9324034631630884	4.346994870255685
5	1.0093048248976293	2.8380737899661006
6	0.5670189547751915	1.9132871587625653
7	0.3557512104265167	1.4004762739816135
8	0.24445645751290518	1.0998230825668849
9	0.16330036994886063	0.8265345405469013
>10	0.7525989164086668	7.415855631685199
>50	0.05010613378485475	1.9526435042879189
>100	0.04264735819478782	4.807832936848423
>500	0.005427741276434349	2.090198939037309
>1k	0.0035893127795775504	3.5081174278907863
>5k	4.3772107068018904E-4	1.6973173577508227
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	7977	0.39060262095974546	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6980	0.3417834141029238	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	6888	0.3372785324270687	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6776	0.33179432864776676	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5854	0.28664757967887045	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4614	0.22592960926517053	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3869	0.18944986091177826	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3826	0.18734431838936771	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3110	0.1522845870859732	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3030	0.14836729867218612	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2721	0.13323677217393348	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2468	0.12084834756533179	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2456	0.12026075430326373	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2407	0.11786141514981915	No Hit
GAATATGGCAAGAAAACTGAAAATC	2066	0.10116397328605166	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	4.896610517233866E-5
3	0.0	0.0	0.0	0.0	4.896610517233866E-5
4	0.0	0.0	0.0	0.0	4.896610517233866E-5
5	0.0	0.0	0.0	0.0	4.896610517233866E-5
6	0.0	0.0	0.0	0.0	9.793221034467731E-5
7	0.0	0.0	4.896610517233866E-5	0.0	9.793221034467731E-5
8	0.0	0.0	4.896610517233866E-5	0.0	9.793221034467731E-5
9	0.0	0.0	4.896610517233866E-5	0.0	9.793221034467731E-5
10	0.0	0.0	4.896610517233866E-5	0.0	9.793221034467731E-5
11	0.0	0.0	4.896610517233866E-5	0.0	9.793221034467731E-5
12	0.0	0.0	4.896610517233866E-5	0.0	9.793221034467731E-5
13	0.0	0.0	4.896610517233866E-5	0.0	9.793221034467731E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1290	0.0	13.326999	4
GGTATCA	2015	0.0	11.912567	1
CCGGGAC	65	8.0377364E-4	11.690151	3
GGACCTG	2155	0.0	11.50396	6
AAATGTC	910	0.0	11.48168	7
CATTTCG	100	1.93294E-6	11.398736	14
ACCTTTT	855	0.0	11.332076	15
AGGACCT	2290	0.0	11.281761	5
GCGCCAC	85	5.3305394E-5	11.175231	13
GGACTCG	60	0.0058837277	11.08156	6
AATGTCC	945	0.0	11.056433	8
CCTATAC	130	3.2763637E-8	10.9595175	3
CACCTTT	890	0.0	10.886433	14
TGTAGGA	3945	0.0	10.85858	2
TTGCGAG	70	0.0014931294	10.855939	18
GGACCGG	80	3.7773434E-4	10.685791	6
GTAAGAC	205	0.0	10.656571	3
GTAGGAC	3955	0.0	10.638998	3
CCAACGA	170	1.4006218E-10	10.61595	19
CGAAATC	215	0.0	10.603474	13
>>END_MODULE