FastQCFastQC Report
Thu 26 May 2016
SRR1512891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042229
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82140.4022075878855897No Hit
GTCCTACAGTGGACATTTCTAAATT74900.3667561277408165No Hit
CTGTAGGACGTGGAATATGGCAAGA68150.3337040067494879No Hit
GTCCTAAAGTGTGTATTTCTCATTT64910.31783898867365024No Hit
CTTTAGGACGTGAAATATGGCGAGG61280.3000642924960913No Hit
TATCAACGCAGAGTACTTTTTTTTT60230.29492285145299574No Hit
GGTATCAACGCAGAGTACTTTTTTT47560.23288279619964264No Hit
GTCCTACAGTGTGCATTTCTCATTT42840.2097707945582988No Hit
CTGAAGGACCTGGAATATGGCGAGA33180.16246953696181965No Hit
CTGTAGGACCTGGAATATGGCGAGA29630.14508656962563943No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27900.13661543343082486No Hit
ATTTAGAAATGTCCACTGTAGGACG24990.12236629682567429No Hit
GTCCTTCAGTGTGCATTTCTCATTT24450.11972212714636801No Hit
TTTCTAAATTTTCCACCTTTTTCAG24450.11972212714636801No Hit
GTACTGGTTCACTATCGGTCAGTCA22660.1109571943205194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11350.013.4901074
TTGGCGA655.491387E-513.14430317
CGGTAGG550.00304019712.1061471
GGTATCA19250.012.0567341
TTAAGAC1459.458745E-1111.8073863
TCCAACG2050.011.57696118
GGCGAGG17400.011.56574719
TTAGGCC1251.8002538E-811.4138073
ATAGGAC3350.011.3570213
CCACCTT8050.011.32069613
AATGTCC8700.011.0282958
GGACCTG20950.010.985426
TAGAAAT9350.010.9849354
TGGCGAA1658.185452E-1110.931457518
AGGACCT22450.010.8868475
GTCGAGG1053.4996774E-610.84873519
CTACACT4050.010.8016024
CTAGCAC1855.456968E-1210.79684453
CGCGCCT1855.456968E-1210.77569612
CACCTTT8700.010.69338514
Produced by FastQC (version 0.11.5)