##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512891_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2042229 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.390724546561625 31.0 31.0 34.0 27.0 34.0 2 30.424933736618176 31.0 30.0 34.0 26.0 34.0 3 30.603589019644712 31.0 31.0 34.0 27.0 34.0 4 34.18514867823344 37.0 35.0 37.0 30.0 37.0 5 33.79461216151568 37.0 35.0 37.0 28.0 37.0 6 33.661062006268644 37.0 35.0 37.0 28.0 37.0 7 33.50360708813752 37.0 35.0 37.0 28.0 37.0 8 33.45442798040768 37.0 35.0 37.0 28.0 37.0 9 34.79424638471004 39.0 35.0 39.0 27.0 39.0 10 34.727779303888056 39.0 35.0 39.0 27.0 39.0 11 34.694511732033966 39.0 35.0 39.0 27.0 39.0 12 34.562366414344325 39.0 35.0 39.0 27.0 39.0 13 34.55309908927941 39.0 35.0 39.0 27.0 39.0 14 35.6405799741361 40.0 36.0 41.0 27.0 41.0 15 35.65380327083789 40.0 36.0 41.0 27.0 41.0 16 35.6650292401097 40.0 36.0 41.0 27.0 41.0 17 35.66283947588639 40.0 36.0 41.0 27.0 41.0 18 35.59718376342712 40.0 36.0 41.0 27.0 41.0 19 35.61141184460705 40.0 36.0 41.0 27.0 41.0 20 35.58792231429482 40.0 36.0 41.0 27.0 41.0 21 35.550732067755376 40.0 36.0 41.0 27.0 41.0 22 35.516479297865224 40.0 36.0 41.0 27.0 41.0 23 35.35473592824311 40.0 36.0 41.0 25.0 41.0 24 35.32112657297492 40.0 36.0 41.0 25.0 41.0 25 35.26293525358811 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8517156225149876 1101 2 0.6983950675428474 1101 3 0.6853253148390515 1101 4 0.7505327864851665 1101 5 0.8084484790287405 1101 6 0.944267442809533 1101 7 1.0136140728027598 1101 8 0.8082038473962356 1101 9 1.1398818274672777 1101 10 0.8501517311504614 1101 11 1.1328490874341313 1101 12 1.2067914968918885 1101 13 1.3390690756432946 1101 14 1.4500108406172885 1101 15 1.0279334376829041 1101 16 1.322837333330014 1101 17 1.4124858911922402 1101 18 1.300027350977821 1101 19 1.1454897584590498 1101 20 1.3400941543593277 1101 21 1.333549864994744 1101 22 1.2405940816881227 1101 23 1.3871474791093235 1101 24 1.4279928093567804 1101 25 1.5070088187083428 1102 1 0.8246265255440015 1102 2 0.8144367278758473 1102 3 0.5684830789023287 1102 4 0.6270363992092527 1102 5 0.7945245438449646 1102 6 0.7002970945084783 1102 7 0.7087265080726795 1102 8 0.808462261040475 1102 9 1.0764780130792104 1102 10 0.8401407359032476 1102 11 1.047649855581355 1102 12 0.9403695631471223 1102 13 0.9569145645517736 1102 14 0.9945833940481137 1102 15 1.0853063336384778 1102 16 1.070278531558614 1102 17 1.1016649465130257 1102 18 0.5194636038786413 1102 19 0.9235580404643358 1102 20 1.112118850596623 1102 21 0.9326970999687489 1102 22 0.7396123631867155 1102 23 0.9354442291777261 1102 24 0.9353005952078774 1102 25 0.9728336142575458 1103 1 0.5373151436187023 1103 2 0.7256860883625755 1103 3 0.48091147488649 1103 4 0.29895535616157076 1103 5 0.5036487572589721 1103 6 0.5016780751077619 1103 7 0.6756813084402538 1103 8 0.6772617428046885 1103 9 0.6721832443897853 1103 10 0.5246708332074164 1103 11 0.7971265895671493 1103 12 0.8739936066377041 1103 13 0.9755435707880267 1103 14 0.9875472144666517 1103 15 0.9032177354392985 1103 16 0.9686517447554692 1103 17 0.8859520594464669 1103 18 0.9519380570871263 1103 19 0.8906882852399605 1103 20 0.7141002068677764 1103 21 0.8716645298799364 1103 22 0.8084255247072178 1103 23 0.8714898349087434 1103 24 0.8689129833007172 1103 25 0.7870827674008893 1104 1 0.6356653316159182 1104 2 0.7753259520101032 1104 3 0.6524812208474913 1104 4 0.5063054506637314 1104 5 0.7339246819205982 1104 6 0.6593943185063651 1104 7 0.6712266146125216 1104 8 0.6145256565078441 1104 9 0.6362958725659453 1104 10 0.71267646936613 1104 11 0.779320741225753 1104 12 0.7711779022336032 1104 13 0.6619611437705402 1104 14 0.9422339774602833 1104 15 0.9183924945392121 1104 16 0.8955894148165129 1104 17 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2216 18 -2.9900904634864744 2216 19 -3.0667665031945646 2216 20 -2.4893723662851457 2216 21 -3.0770883216721856 2216 22 -2.4766168660866583 2216 23 -3.599127589586743 2216 24 -2.7825037106637396 2216 25 -2.9557696019344277 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51075.0 3 1.0 4 871.0 5 7483.0 6 9692.0 7 7359.0 8 5917.0 9 4963.0 10 4326.0 11 3918.0 12 3602.0 13 3278.0 14 3096.0 15 3239.0 16 3183.0 17 3496.0 18 3742.0 19 4631.0 20 6163.0 21 8277.0 22 10310.0 23 12701.0 24 14758.0 25 16767.0 26 18989.0 27 20660.0 28 23357.0 29 27869.0 30 35108.0 31 44264.0 32 56536.0 33 73444.0 34 95849.0 35 125831.0 36 173740.0 37 269496.0 38 884238.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.15645806081986 19.75770662790339 13.733796832905398 26.352038478371355 2 12.736799460353678 20.60370237741245 42.50856179063035 24.150936371603514 3 17.422103809346563 26.025065872603854 29.7840162208226 26.768814097226983 4 11.105561350158903 17.233574747934753 38.15281103553643 33.50805286636992 5 11.89270511537158 39.724348212764035 35.53222353035667 12.850723141507714 6 30.97932653723173 37.43585170609719 18.556875295814645 13.027946460856436 7 27.767751659781233 31.918858515440903 22.22987179908084 18.083518025697025 8 24.795028560790882 38.326229877624606 20.460303929925633 16.41843763165888 9 27.186562898991074 14.494514584432203 20.295915163999258 38.023007352577466 10 15.491824158167514 29.42313533002185 34.09477902203412 20.990261489776525 11 33.73890560614259 22.114306840634924 25.697027865444745 18.449759687777743 12 24.322258478991532 24.85647999435909 31.83725193710386 18.984009589545515 13 29.558617211162012 22.487727124707106 25.9587043761217 21.994951288009187 14 20.237956500321708 23.53607981894231 27.743604759913467 28.482358920822513 15 22.387951522832818 33.35736263994581 23.159370621528137 21.095315215693226 16 20.835130975251552 28.0769549080399 29.79470888484824 21.293205231860306 17 20.55363762346178 29.765783764990477 28.55283454040634 21.127744071141404 18 20.42364149915969 27.065124988737104 32.358097334905565 20.153136177197638 19 23.321576398774067 26.57669502335473 28.232214392426986 21.86951418544422 20 23.95127657584561 27.207535325510808 29.048178429268916 19.793009669374666 21 22.411133733484935 26.53898246325333 27.76029685255207 23.28958695070967 22 23.13655266834229 29.147012838460835 27.62705150221107 20.089382990985804 23 21.000974915153982 28.722097277043858 29.19907610752761 21.077851700274554 24 23.08869377528181 26.678398945465958 29.715178856044055 20.51772842320817 25 21.689976981033958 28.040195296413867 29.403019935570402 20.866807786981774 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 105.0 1 105.0 2 364.0 3 623.0 4 623.0 5 623.0 6 1475.0 7 2327.0 8 2327.0 9 2327.0 10 2817.5 11 3308.0 12 3308.0 13 3308.0 14 4989.0 15 6670.0 16 6670.0 17 6670.0 18 13630.5 19 20591.0 20 20591.0 21 20591.0 22 36232.0 23 51873.0 24 51873.0 25 51873.0 26 80703.0 27 109533.0 28 109533.0 29 109533.0 30 143483.5 31 177434.0 32 177434.0 33 177434.0 34 198379.0 35 219324.0 36 219324.0 37 219324.0 38 230226.5 39 241129.0 40 241129.0 41 241129.0 42 250854.0 43 260579.0 44 260579.0 45 260579.0 46 271962.0 47 283345.0 48 283345.0 49 283345.0 50 264012.5 51 244680.0 52 244680.0 53 244680.0 54 207724.5 55 170769.0 56 170769.0 57 170769.0 58 142598.0 59 114427.0 60 114427.0 61 114427.0 62 92948.5 63 71470.0 64 71470.0 65 71470.0 66 54013.0 67 36556.0 68 36556.0 69 36556.0 70 26783.5 71 17011.0 72 17011.0 73 17011.0 74 11914.5 75 6818.0 76 6818.0 77 6818.0 78 4683.5 79 2549.0 80 2549.0 81 2549.0 82 1688.5 83 828.0 84 828.0 85 828.0 86 522.0 87 216.0 88 216.0 89 216.0 90 137.5 91 59.0 92 59.0 93 59.0 94 32.0 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08862865036193296 2 0.04367776581372608 3 0.020027137015486512 4 0.004847644412061527 5 0.01493466207756329 6 0.061452461991285005 7 0.07090292028954637 8 0.04744815591199616 9 0.017627797862041914 10 0.04157222329131552 11 0.004602813886199834 12 2.448305258616933E-4 13 0.006071797041369994 14 3.427627362063706E-4 15 0.013612577237910147 16 0.009891153244812408 17 0.004847644412061527 18 0.0061207631465423325 19 0.0014689831551701596 20 3.9172884137870926E-4 21 1.4689831551701596E-4 22 2.937966310340319E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2042229.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.42614394878756 #Duplication Level Percentage of deduplicated Percentage of total 1 75.99022337771723 42.11845059633877 2 14.063195310529766 15.589373753226743 3 4.702957374018649 7.8200037719210895 4 1.9807993536706463 4.391522804408584 5 1.0262550540016588 2.844068017563335 6 0.5862606837607975 1.9496501429784356 7 0.36196572194638355 1.4043654946388948 8 0.24847790188402002 1.1017737566333123 9 0.161300049361841 0.8046215779388346 >10 0.7735182594623523 7.48866649196141 >50 0.052138843979733604 2.0173501330373615 >100 0.04344351382270271 4.928304819318795 >500 0.005395681369074081 2.0788718074482384 >1k 0.0035381517174256217 3.444995971175923 >5k 5.307227576138433E-4 2.0179808614102033 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8214 0.4022075878855897 No Hit GTCCTACAGTGGACATTTCTAAATT 7490 0.3667561277408165 No Hit CTGTAGGACGTGGAATATGGCAAGA 6815 0.3337040067494879 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6491 0.31783898867365024 No Hit CTTTAGGACGTGAAATATGGCGAGG 6128 0.3000642924960913 No Hit TATCAACGCAGAGTACTTTTTTTTT 6023 0.29492285145299574 No Hit GGTATCAACGCAGAGTACTTTTTTT 4756 0.23288279619964264 No Hit GTCCTACAGTGTGCATTTCTCATTT 4284 0.2097707945582988 No Hit CTGAAGGACCTGGAATATGGCGAGA 3318 0.16246953696181965 No Hit CTGTAGGACCTGGAATATGGCGAGA 2963 0.14508656962563943 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2790 0.13661543343082486 No Hit ATTTAGAAATGTCCACTGTAGGACG 2499 0.12236629682567429 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2445 0.11972212714636801 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2445 0.11972212714636801 No Hit GTACTGGTTCACTATCGGTCAGTCA 2266 0.1109571943205194 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.896610517233866E-5 7 0.0 0.0 0.0 0.0 4.896610517233866E-5 8 0.0 0.0 0.0 0.0 4.896610517233866E-5 9 0.0 0.0 0.0 0.0 4.896610517233866E-5 10 0.0 0.0 0.0 0.0 4.896610517233866E-5 11 0.0 0.0 0.0 0.0 4.896610517233866E-5 12 0.0 0.0 0.0 0.0 9.793221034467731E-5 13 0.0 0.0 0.0 0.0 1.4689831551701596E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 1135 0.0 13.490107 4 TTGGCGA 65 5.491387E-5 13.144303 17 CGGTAGG 55 0.003040197 12.106147 1 GGTATCA 1925 0.0 12.056734 1 TTAAGAC 145 9.458745E-11 11.807386 3 TCCAACG 205 0.0 11.576961 18 GGCGAGG 1740 0.0 11.565747 19 TTAGGCC 125 1.8002538E-8 11.413807 3 ATAGGAC 335 0.0 11.357021 3 CCACCTT 805 0.0 11.320696 13 AATGTCC 870 0.0 11.028295 8 GGACCTG 2095 0.0 10.98542 6 TAGAAAT 935 0.0 10.984935 4 TGGCGAA 165 8.185452E-11 10.9314575 18 AGGACCT 2245 0.0 10.886847 5 GTCGAGG 105 3.4996774E-6 10.848735 19 CTACACT 405 0.0 10.801602 4 CTAGCAC 185 5.456968E-12 10.7968445 3 CGCGCCT 185 5.456968E-12 10.775696 12 CACCTTT 870 0.0 10.693385 14 >>END_MODULE