FastQCFastQC Report
Thu 26 May 2016
SRR1512890_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512890_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785629
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT142420.7975900929028371No Hit
TATCAACGCAGAGTACTTTTTTTTT84270.471934539593611No Hit
GGTATCAACGCAGAGTACTTTTTTT80620.4514935633325848No Hit
GTCCTACAGTGGACATTTCTAAATT41090.23011499029193636No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39620.22188259711283811No Hit
GTCCTAAAGTGTGTATTTCTCATTT38860.21762639383656962No Hit
CTGTAGGACGTGGAATATGGCAAGA36690.20547381342932938No Hit
CTTTAGGACGTGAAATATGGCGAGG33990.19035309126363875No Hit
GTACTTTTTTTTTTTTTTTTTTTTT31770.1779204974829598No Hit
GTACTGGTTCACTATCGGTCAGTCA31640.17719246271201913No Hit
GTACATGGGAAGCAGTGGTATCAAC28480.1594956175106923No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27850.1559674490053645No Hit
GTCCTACAGTGTGCATTTCTCATTT21720.12163780942177797No Hit
CCCATGTACTCTGCGTTGATACCAC19510.10926121831578675No Hit
GGATACCACGTGTCCCGCCCTACTC18570.1039969668951389No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACCG456.769759E-414.7758375
TAGGACC6800.013.689384
TAGAACG550.0030717712.0893224
CGTGCGC1302.6229827E-911.69044510
GGCGAGG10900.011.50243219
GGTATCA26800.011.2676791
TGTAGGA24250.011.2418232
TTTCGCC600.00588198211.0818783
TTTTCGC600.00588198211.0818782
TTAGGAC15450.011.0665083
AGGACCT12750.011.0260035
GTAGGAC23600.010.9074663
GTCTTAG1158.512543E-710.7685081
CTTAGAC1158.8323213E-710.73772053
GTCCTAA10950.010.7004241
TGTCCCG3300.010.65011711
GTCCTAC21650.010.6479861
TTTAGGA17050.010.6408312
TAGAACC1252.2442327E-710.6386024
CGAATGG1252.2459608E-710.63800619
Produced by FastQC (version 0.11.5)