##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512890_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1785629
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.640076410049343	31.0	30.0	33.0	22.0	34.0
2	28.76960051612065	31.0	30.0	34.0	19.0	34.0
3	28.884589688003498	31.0	30.0	34.0	22.0	34.0
4	32.28439670278652	35.0	33.0	37.0	22.0	37.0
5	32.483401647262674	35.0	33.0	37.0	25.0	37.0
6	32.44136491958856	35.0	33.0	37.0	23.0	37.0
7	32.406226601382485	36.0	33.0	37.0	23.0	37.0
8	32.304519023828576	36.0	33.0	37.0	22.0	37.0
9	33.50973578498109	38.0	34.0	39.0	18.0	39.0
10	33.37620860772311	38.0	33.0	39.0	17.0	39.0
11	33.406114596033106	38.0	34.0	39.0	17.0	39.0
12	33.29634431340441	38.0	33.0	39.0	17.0	39.0
13	33.23559877219736	38.0	33.0	39.0	17.0	39.0
14	34.08899497039979	39.0	33.0	41.0	16.0	41.0
15	34.24494449854925	39.0	34.0	41.0	17.0	41.0
16	34.19025340650269	39.0	34.0	41.0	17.0	41.0
17	33.99744011774003	39.0	33.0	41.0	13.0	41.0
18	33.98870034032825	39.0	33.0	41.0	12.0	41.0
19	34.114306499278406	39.0	34.0	41.0	13.0	41.0
20	34.102035753227575	39.0	34.0	41.0	11.0	41.0
21	34.012478515973925	39.0	34.0	41.0	10.0	41.0
22	34.02876185366613	39.0	34.0	41.0	10.0	41.0
23	33.71721057397701	39.0	33.0	41.0	2.0	41.0
24	33.781753656554635	39.0	34.0	41.0	2.0	41.0
25	33.52262648064072	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.37242057246328386
1101	2	0.0706163390877883
1101	3	0.3567592267577382
1101	4	0.48619165278052634
1101	5	0.3394777790284067
1101	6	0.23853823123400986
1101	7	-0.041622420620214484
1101	8	0.3457303721519196
1101	9	0.38463453390147606
1101	10	0.31517677497987506
1101	11	0.20697074576464303
1101	12	0.2790513674111068
1101	13	0.5971570342580605
1101	14	0.644610358355088
1101	15	0.4181855207925409
1101	16	0.2706898576990895
1101	17	-0.33369991888044126
1101	18	0.11413741920453901
1101	19	-0.45724730358760723
1101	20	-0.960192713467201
1101	21	0.06875399590013842
1101	22	0.12328756156880871
1101	23	-0.3081140070059405
1101	24	0.15095588709800012
1101	25	-0.1955380481287392
1102	1	0.8704409525696981
1102	2	0.8850648081043246
1102	3	0.7552356314597226
1102	4	0.9494287051808641
1102	5	0.838850869621595
1102	6	0.7260027227432317
1102	7	0.6547566639567961
1102	8	0.7501942529572077
1102	9	0.9997703447072865
1102	10	1.1506377467111477
1102	11	0.8077903599518592
1102	12	0.9235465209535079
1102	13	0.8087145232415267
1102	14	0.9786337641638951
1102	15	0.9245048901112156
1102	16	1.0655197499850075
1102	17	1.1205815035977977
1102	18	1.1550605799916838
1102	19	0.9081157028621689
1102	20	1.1201924357852135
1102	21	1.1715781124019315
1102	22	0.9595184847270417
1102	23	0.8838989144990776
1102	24	0.2352336678106326
1102	25	0.756136845049113
1103	1	0.37615802826983824
1103	2	0.419722123909132
1103	3	0.5422113096758245
1103	4	0.653310460689724
1103	5	0.4973498801132905
1103	6	0.13903685452351766
1103	7	-0.029197285117312788
1103	8	0.14102500949812224
1103	9	0.29422264159781975
1103	10	0.41330790760510183
1103	11	0.1510586503397633
1103	12	0.28823163508480576
1103	13	0.48825122113668584
1103	14	0.5218097605041692
1103	15	0.45697672891495955
1103	16	0.4036015052936648
1103	17	0.08062628923435966
1103	18	0.5030624438909967
1103	19	0.4479638080813899
1103	20	0.4524735302204448
1103	21	0.33008237824882514
1103	22	0.28593985810651645
1103	23	-0.3150605752209472
1103	24	-0.12930930147507524
1103	25	0.1610747267505701
1104	1	0.5827619056703703
1104	2	0.6340928630597951
1104	3	0.6236272147505986
1104	4	0.7668376394861056
1104	5	0.4730408396085366
1104	6	0.48886766100769563
1104	7	0.721250139373204
1104	8	0.4444353101605074
1104	9	0.721345015573462
1104	10	0.7236944931728573
1104	11	0.3694988276097604
1104	12	0.5915694733674428
1104	13	0.7040433282886482
1104	14	0.9104751213741906
1104	15	0.710773266343125
1104	16	0.7215898857668464
1104	17	0.9607342115094966
1104	18	0.7297241536396157
1104	19	0.4633130595572865
1104	20	0.5152446193521243
1104	21	0.6005703178068558
1104	22	0.3754351957050588
1104	23	0.8413236797601655
1104	24	0.5718425801641374
1104	25	0.26585864393417324
1105	1	0.6997530101849421
1105	2	0.7296586590937579
1105	3	0.8581626160556475
1105	4	1.07829629313445
1105	5	0.6837905333663841
1105	6	0.9504579453718094
1105	7	0.9981166922087681
1105	8	1.023057147248558
1105	9	0.7823265123780487
1105	10	0.8109933713531703
1105	11	0.9202558092467541
1105	12	1.040415091074017
1105	13	1.0395122550620215
1105	14	1.093756640191252
1105	15	1.073397824623477
1105	16	1.0248808817073822
1105	17	1.4622560003717595
1105	18	1.0806367348571015
1105	19	0.8225573717828993
1105	20	1.1417739397429898
1105	21	0.9565830941599387
1105	22	1.0446591013889588
1105	23	1.1843253353761938
1105	24	1.2377820192326965
1105	25	1.4764048964015757
1106	1	0.6731587749719026
1106	2	0.8197280215559708
1106	3	0.64352313625702
1106	4	0.8932202366891033
1106	5	0.7484896753349943
1106	6	0.8350164086814118
1106	7	0.7717191492253832
1106	8	0.7948746235021389
1106	9	0.9789471865043993
1106	10	0.7315049469863908
1106	11	0.4195715137072824
1106	12	0.7787514176915522
1106	13	0.8477070482616611
1106	14	1.2355789665909427
1106	15	0.872263779381818
1106	16	1.0220359999303028
1106	17	1.2484803154778916
1106	18	1.0061459635666452
1106	19	1.0354099170838964
1106	20	1.0402530062095252
1106	21	1.1438214463606542
1106	22	1.0209588355345218
1106	23	1.2162676748072911
1106	24	0.9766429235871286
1106	25	1.1587594005657138
1107	1	0.8162703081825633
1107	2	0.7975300112563275
1107	3	0.7366380426088455
1107	4	0.7176639723368652
1107	5	0.7148271567976181
1107	6	0.915010153471215
1107	7	0.5762636166421515
1107	8	0.7863228167023166
1107	9	0.597377338524602
1107	10	0.8531370800774241
1107	11	0.7504572338300122
1107	12	0.8351824007202353
1107	13	0.6363062125331567
1107	14	0.9940559802429831
1107	15	0.739356534565907
1107	16	0.7901895186171473
1107	17	0.9686490535869723
1107	18	0.944772792363402
1107	19	0.7912944989204718
1107	20	0.9944519471623892
1107	21	0.792916550585943
1107	22	0.755652343346263
1107	23	0.8789728672753938
1107	24	0.8484040823076029
1107	25	1.1025993415607758
1108	1	0.7297583902948723
1108	2	0.6581499091383556
1108	3	0.7424789852983196
1108	4	0.7453078627181426
1108	5	0.6755405620039667
1108	6	0.7271200049868085
1108	7	0.7038063697214128
1108	8	0.7016915852101633
1108	9	0.8172066194919978
1108	10	0.9371205654145811
1108	11	0.6935768957097252
1108	12	0.8421270551991071
1108	13	0.868349644290916
1108	14	0.8093323870688138
1108	15	0.78025413155828
1108	16	0.6138921245409463
1108	17	0.9219943749213044
1108	18	1.0045180381769967
1108	19	0.9008323376525311
1108	20	0.9024207829423574
1108	21	0.9567570322689605
1108	22	1.0032336633278263
1108	23	1.1272119004999368
1108	24	1.0654717925169237
1108	25	1.2205186993411985
1109	1	0.4160811311165382
1109	2	0.4594649615475461
1109	3	0.5395855824941371
1109	4	0.5428225300575065
1109	5	0.34055995461010724
1109	6	0.4425100922210774
1109	7	0.4093666036568919
1109	8	0.21739648928586064
1109	9	0.4147163714410027
1109	10	0.46861500174914283
1109	11	0.3218790178599562
1109	12	0.34888571695161374
1109	13	0.3700171672865906
1109	14	0.4268913671160348
1109	15	0.211866212660361
1109	16	-0.12417877950136358
1109	17	0.4064608139342951
1109	18	0.5038994409366637
1109	19	0.34369370695044665
1109	20	0.2574721812318046
1109	21	0.27236798746842084
1109	22	0.2820304314122879
1109	23	0.5586229606847155
1109	24	0.39034176653027686
1109	25	-0.067884410895239
1110	1	0.4293699240594684
1110	2	0.4680115074814424
1110	3	0.36094556326793636
1110	4	0.34923016113835104
1110	5	0.18807689029786445
1110	6	0.31711533867012065
1110	7	0.21953042189898042
1110	8	0.26862788443217056
1110	9	0.5012680463664694
1110	10	0.4231407111258747
1110	11	0.41047486447317993
1110	12	0.4427044655970249
1110	13	0.42752298406820444
1110	14	0.35243771898373666
1110	15	0.418809017719596
1110	16	0.44940584990042964
1110	17	0.5333665775616652
1110	18	-0.03434062149760564
1110	19	0.32096263343590437
1110	20	0.3993604800576662
1110	21	0.4728723745442167
1110	22	0.6221147935393532
1110	23	0.5610209157497152
1110	24	0.4088757825880407
1110	25	0.5862512231056201
1111	1	0.4872473733742275
1111	2	0.48749276243670536
1111	3	0.5720428311100214
1111	4	0.4143816190862779
1111	5	0.5005509341684444
1111	6	0.2932514305894216
1111	7	0.356975206132752
1111	8	0.45943847075753297
1111	9	0.49730383750929263
1111	10	0.6547795024826399
1111	11	0.4420280792577884
1111	12	0.12073834385176951
1111	13	0.45854449147435616
1111	14	0.48255500922674344
1111	15	0.4250542197683984
1111	16	0.656252604912325
1111	17	0.1693798419303505
1111	18	0.530298006256892
1111	19	0.5043072930802595
1111	20	0.6834872844019415
1111	21	0.625258450732531
1111	22	0.2672772002545969
1111	23	0.5912527275819386
1111	24	0.534031519364575
1111	25	0.633167320310541
1112	1	0.28206506912992424
1112	2	0.23299552898728493
1112	3	0.3571354684231025
1112	4	0.37019495294683935
1112	5	0.27331107324445014
1112	6	0.0757895865801359
1112	7	-0.13775013004762826
1112	8	0.15139951591769574
1112	9	-0.03328073928896913
1112	10	3.1988131695470656E-4
1112	11	0.05752062129269575
1112	12	0.1800040522760753
1112	13	0.12851058863738274
1112	14	-0.045942533029403876
1112	15	-0.17096043160480434
1112	16	0.05897636504857928
1112	17	0.34512876887401944
1112	18	0.23100802637640783
1112	19	0.3461330469970676
1112	20	0.1935837582621076
1112	21	0.22789126351938904
1112	22	0.1099069814631406
1112	23	0.2890709797546478
1112	24	0.26995092080673544
1112	25	0.27083157395384916
1113	1	0.008943944785549718
1113	2	0.07724796180885463
1113	3	0.0645816176812275
1113	4	0.1383988925919155
1113	5	0.00886762979836675
1113	6	-0.3714782432636774
1113	7	-0.47944993713835515
1113	8	-0.24806279773259376
1113	9	-0.3536467754625079
1113	10	-0.1763509222591182
1113	11	-0.2282654630134786
1113	12	-0.44176348282193345
1113	13	-0.34936609566288723
1113	14	-0.009449430368924538
1113	15	-0.4306381985655108
1113	16	-0.48041121573682943
1113	17	-0.3201388840503867
1113	18	-0.4166008887312884
1113	19	-0.8887676132383362
1113	20	-0.5258526359766478
1113	21	-0.29985211992272554
1113	22	-0.7253129727439784
1113	23	-4.545413967832381
1113	24	-2.0159398048528807
1113	25	-1.1659694835351289
1114	1	-0.48335660162728544
1114	2	-0.7496159381724361
1114	3	-0.5855399865920177
1114	4	-0.5886084995102117
1114	5	-0.6009453143521384
1114	6	-1.060280973392505
1114	7	-0.9720094513479474
1114	8	-0.8339106223570205
1114	9	-1.1126601911002965
1114	10	-0.622467649459125
1114	11	-0.8104398156170234
1114	12	-1.714133093457857
1114	13	-0.8224661613725104
1114	14	-2.7025191387859344
1114	15	-1.487290399284909
1114	16	-1.3150552087437717
1114	17	-1.0551180606433874
1114	18	-1.2080083188030741
1114	19	-1.3121071550404366
1114	20	-0.9758124084167648
1114	21	-0.8658948984536536
1114	22	-1.0034923093256225
1114	23	-5.124852109088717
1114	24	-2.649273660422576
1114	25	-2.548437271535427
1115	1	-1.3547911290253047
1115	2	-1.3383763806925764
1115	3	-0.9585232058578192
1115	4	-1.1114321659043398
1115	5	-0.9295176936416958
1115	6	-1.1163255273994466
1115	7	-1.593046774304657
1115	8	-1.9522108557862836
1115	9	-2.8849588653518268
1115	10	-3.5839026074765314
1115	11	-2.4786026982391256
1115	12	-2.713820195344642
1115	13	-2.054462635885997
1115	14	-2.758395359759671
1115	15	-2.3718806285869363
1115	16	-2.492458791944472
1115	17	-2.463943213513268
1115	18	-5.159023266329022
1115	19	-3.042121801692062
1115	20	-3.157638928083763
1115	21	-2.661491619482753
1115	22	-2.3074445843941014
1115	23	-2.6959644269283274
1115	24	-2.486886530237946
1115	25	-2.875400979828264
1116	1	-0.48631960371162464
1116	2	-0.2781762975381774
1116	3	-0.670213803145618
1116	4	-0.22004120923006099
1116	5	-0.4100120066781443
1116	6	-2.517912865148695
1116	7	-1.0235074838508957
1116	8	-1.5041958822988697
1116	9	-2.2893362895019145
1116	10	-1.728934416882094
1116	11	-1.2044060494753381
1116	12	-0.968362362029346
1116	13	-2.4498133936731783
1116	14	-5.12800385062123
1116	15	-3.0947465221834776
1116	16	-2.0158167773298032
1116	17	-3.0172481737301524
1116	18	-1.611494973898921
1116	19	-1.2910944746503645
1116	20	-2.1700693717067914
1116	21	-2.904991622330506
1116	22	-2.394409989885709
1116	23	-1.3530692869042937
1116	24	-2.3675229461694194
1116	25	-2.1423066679309173
1201	1	0.49959305238657237
1201	2	0.43048477405616836
1201	3	0.9179235702369475
1201	4	0.8873098850428676
1201	5	0.3196020113894562
1201	6	0.8711784861853289
1201	7	1.018921466118229
1201	8	1.014025737763106
1201	9	1.432670576797598
1201	10	1.2667176933968634
1201	11	1.4925606379602812
1201	12	1.292024553809
1201	13	1.3204826823885796
1201	14	1.6400027619904023
1201	15	1.530093354763956
1201	16	1.4676178917678087
1201	17	1.6631061390335518
1201	18	1.4338524321424728
1201	19	1.5157452015769906
1201	20	1.432256751036327
1201	21	1.7570062115652618
1201	22	1.45474189806788
1201	23	1.8642495806441488
1201	24	1.6970671039687844
1201	25	1.8090730735583378
1202	1	0.8783664494395396
1202	2	0.7598543713574522
1202	3	0.7444000150562147
1202	4	1.0692645785092623
1202	5	0.8356232825024392
1202	6	1.0806778653874218
1202	7	1.110141275427857
1202	8	1.0721956724036232
1202	9	1.2092114440405553
1202	10	1.1640962864111302
1202	11	1.1245914670183126
1202	12	1.3519631768089795
1202	13	1.2122061579197734
1202	14	1.7963056176607495
1202	15	1.301196969447588
1202	16	1.1855835487593964
1202	17	1.5938742885872799
1202	18	1.4112108785078803
1202	19	1.5211730836019584
1202	20	1.2539284543652087
1202	21	1.3336946647243053
1202	22	1.312675226715946
1202	23	1.432424903341925
1202	24	1.4306464138289314
1202	25	1.7903999412229723
1203	1	0.9722077358736279
1203	2	1.1578731345900017
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2211	15	0.01982729547829365
2211	16	-0.17501101164540955
2211	17	0.3331398090672195
2211	18	0.030201544177863582
2211	19	0.07790932648621407
2211	20	-0.00960632416008167
2211	21	0.13238747518182237
2211	22	0.18295870667684255
2211	23	0.28984540820076177
2211	24	-0.15472533017658918
2211	25	0.27613585826818365
2212	1	-0.17310879400258727
2212	2	-0.08171951769002916
2212	3	-0.4121775216583501
2212	4	-0.27715024053473414
2212	5	-0.4368124075410158
2212	6	-0.4682046797326329
2212	7	-0.3568825454241136
2212	8	-0.2936450355609743
2212	9	-0.1977819267210137
2212	10	-0.5952515741528259
2212	11	-0.6495437186955257
2212	12	-0.24355812239488017
2212	13	-0.28896172992239144
2212	14	-0.2857294633808962
2212	15	-0.7444305878842243
2212	16	-0.6970446746681418
2212	17	-0.39415184774031076
2212	18	-0.24154644061541575
2212	19	-0.34732058848862835
2212	20	-0.33610167853674966
2212	21	-0.18756393901217905
2212	22	-0.04980074625959929
2212	23	0.15377612610336655
2212	24	-7.133730204387234E-4
2212	25	0.1404205584716749
2213	1	-0.41835155889137
2213	2	-0.30066873239145764
2213	3	-0.5470288331468289
2213	4	-0.3656458157559719
2213	5	-0.34502575363046617
2213	6	-0.09565265868503303
2213	7	-0.46550727634972944
2213	8	-0.19032685011889328
2213	9	-0.7061929926542021
2213	10	-0.3267416482977694
2213	11	-0.4614164764089921
2213	12	-0.4834528844135306
2213	13	-0.5680050210010847
2213	14	-0.5903997974317647
2213	15	-0.47060773848615156
2213	16	-0.48487852621880734
2213	17	-0.4499598681501027
2213	18	-0.4833004069711464
2213	19	-0.46680715470085943
2213	20	-0.2537417965308464
2213	21	-0.5947137436841032
2213	22	-0.6488185251003884
2213	23	-0.380571318686556
2213	24	-0.23242827376189013
2213	25	-0.08328987762813966
2214	1	-2.6379327160335038
2214	2	-1.2417454188339967
2214	3	-1.4477045933347839
2214	4	-3.2776908438985615
2214	5	-1.7257934631786647
2214	6	-1.3836035739530246
2214	7	-1.1841199227672519
2214	8	-1.1047274045194513
2214	9	-0.8998795873164127
2214	10	-0.8975524591085815
2214	11	-0.8990859657857939
2214	12	-0.9044457346746739
2214	13	-1.2522279305898465
2214	14	-1.0674452481357477
2214	15	-1.3602103617473134
2214	16	-1.2450989342440906
2214	17	-3.3534671277549215
2214	18	-3.036659093988373
2214	19	-2.053076951702362
2214	20	-1.7666623718904475
2214	21	-1.822944901671363
2214	22	-3.011105765436408
2214	23	-1.8089275548476706
2214	24	-2.8097296276486077
2214	25	-2.9948697501836214
2215	1	-1.8896640570137997
2215	2	-3.628526430771572
2215	3	-2.044313579916569
2215	4	-3.788083755733741
2215	5	-2.485073218657554
2215	6	-2.338906316195825
2215	7	-2.5790436211420946
2215	8	-1.9640029196675108
2215	9	-4.502101520670919
2215	10	-2.874432785155264
2215	11	-2.2335326756018823
2215	12	-2.1063903086847837
2215	13	-2.3091431802792926
2215	14	-2.1165703844693375
2215	15	-2.1104612711592523
2215	16	-2.351984327833687
2215	17	-2.605958252603468
2215	18	-2.279154994422626
2215	19	-2.4885764452956387
2215	20	-2.1445260376077897
2215	21	-2.8299791239216994
2215	22	-2.6316056100734677
2215	23	-2.545835463898829
2215	24	-2.5319554259603976
2215	25	-3.3347210049392224
2216	1	-2.393985876482983
2216	2	-2.3426003793408974
2216	3	-3.7367553568235294
2216	4	-4.2154322926243
2216	5	-3.6164877138112885
2216	6	-2.48138989057885
2216	7	-4.143601438127163
2216	8	-2.2616121567778684
2216	9	-2.704312963158518
2216	10	-1.8931930285035996
2216	11	-2.2385238150643687
2216	12	-2.6475482965516512
2216	13	-1.997450534129868
2216	14	-2.9031095534511273
2216	15	-2.4863140319682486
2216	16	-2.789757016809041
2216	17	-2.448408138484492
2216	18	-2.046550062320449
2216	19	-2.695068832749012
2216	20	-3.047537763316136
2216	21	-2.9067462210012813
2216	22	-2.568424340880579
2216	23	-2.6179300118993396
2216	24	-2.8557854921902788
2216	25	-3.0526360031130544
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92534.0
3	2.0
4	755.0
5	5033.0
6	7322.0
7	6765.0
8	6200.0
9	5435.0
10	4766.0
11	4455.0
12	4329.0
13	3792.0
14	3781.0
15	3783.0
16	3734.0
17	4079.0
18	4486.0
19	5493.0
20	7420.0
21	9528.0
22	11913.0
23	14209.0
24	16812.0
25	18614.0
26	20316.0
27	22210.0
28	24628.0
29	28905.0
30	34220.0
31	42622.0
32	53443.0
33	67177.0
34	87004.0
35	114457.0
36	157816.0
37	251624.0
38	635967.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.785882458713324	18.962143816411274	12.846996285744789	23.404977439130604
2	14.753898198763329	20.730072139080562	42.09091316426203	22.425116497894077
3	19.446897909050737	26.70481562983595	28.96395712694285	24.88432933417047
4	12.07176306449255	17.01297893897888	38.43951404450802	32.475743952020544
5	12.557326217825066	39.65641235893886	34.53962034192975	13.246641081306333
6	30.578944094122342	37.14603097917254	18.723808744495525	13.551216182209592
7	27.41721787495334	32.78397455082269	21.869770246746274	17.92903732747769
8	25.36553453083428	36.636701113225165	20.831964552596496	17.165799803344047
9	27.74855171238712	14.862883482347033	20.15832518764965	37.2302396176162
10	16.347010259235866	29.258916925581165	32.86901551581205	21.525057299370925
11	33.587650199593526	23.355469288604056	24.728018088408554	18.32886242339386
12	24.236900211385915	25.647541034148553	31.00646265767758	19.109096096787955
13	29.235809267588664	22.983900121905084	25.897571706811274	21.882718903694983
14	21.20175349603412	23.87790322589686	27.758194219695948	27.162149058373082
15	23.242074088024086	31.907050311969638	23.859735851510077	20.991139748496202
16	21.11867910378326	28.700727865022685	28.671581876127306	21.50901115506675
17	20.80670657877035	29.325754518139828	28.012130588815644	21.855408314274182
18	20.59065731549877	27.22244863294947	31.46281946569186	20.7240745858599
19	22.96199384071741	26.97214197067894	28.92542155883438	21.140442629769268
20	23.602195500927124	26.981769180924793	29.42911818978796	19.986917128360123
21	22.86847404695542	26.654197980835143	28.47136788807103	22.005960084138408
22	23.053055550105526	28.341905447940867	28.16588513272997	20.439153869223635
23	21.642216475977413	27.97938526889791	29.396171565953853	20.98222668917082
24	22.687580119626624	26.83678885861447	29.771290170516423	20.70434085124248
25	22.07289416422935	27.40873936278142	29.577605312807936	20.940761160181292
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	560.0
1	560.0
2	1175.0
3	1790.0
4	1790.0
5	1790.0
6	3606.0
7	5422.0
8	5422.0
9	5422.0
10	6406.5
11	7391.0
12	7391.0
13	7391.0
14	8919.0
15	10447.0
16	10447.0
17	10447.0
18	17786.5
19	25126.0
20	25126.0
21	25126.0
22	39040.5
23	52955.0
24	52955.0
25	52955.0
26	76575.5
27	100196.0
28	100196.0
29	100196.0
30	125295.0
31	150394.0
32	150394.0
33	150394.0
34	162355.0
35	174316.0
36	174316.0
37	174316.0
38	179918.0
39	185520.0
40	185520.0
41	185520.0
42	195016.0
43	204512.0
44	204512.0
45	204512.0
46	220521.0
47	236530.0
48	236530.0
49	236530.0
50	224721.5
51	212913.0
52	212913.0
53	212913.0
54	185455.5
55	157998.0
56	157998.0
57	157998.0
58	134994.0
59	111990.0
60	111990.0
61	111990.0
62	93866.0
63	75742.0
64	75742.0
65	75742.0
66	57662.5
67	39583.0
68	39583.0
69	39583.0
70	29647.5
71	19712.0
72	19712.0
73	19712.0
74	13945.5
75	8179.0
76	8179.0
77	8179.0
78	5604.0
79	3029.0
80	3029.0
81	3029.0
82	1974.0
83	919.0
84	919.0
85	919.0
86	609.0
87	299.0
88	299.0
89	299.0
90	187.0
91	75.0
92	75.0
93	75.0
94	48.0
95	21.0
96	21.0
97	21.0
98	15.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3359600454517708
2	0.010584505515983444
3	0.009632460046291811
4	0.011088529588173131
5	0.01304862320224414
6	0.07829173921346484
7	0.0765556562981448
8	0.10097282246200077
9	0.0813718863212907
10	0.0883722206572586
11	0.08417202005567785
12	0.09453251487291033
13	0.08142788899597844
14	0.08602010832037338
15	0.08293996121254751
16	0.08439603075442884
17	0.09570857104135293
18	0.09811668605292588
19	0.09268442660821481
20	0.09055632497008057
21	0.07985981410472164
22	0.09766866465542394
23	0.093076445331029
24	0.0883722206572586
25	0.08753218053694245
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1785629.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.679079902210354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.28240797959184	38.38846497305915
2	14.174636961987492	14.650643922867518
3	5.100475171702493	7.907635918129599
4	2.2869456900720233	4.7274899619699084
5	1.2706578934307182	3.283321540149019
6	0.7311688245312228	2.2671679262972573
7	0.4756812630230834	1.7207938999827987
8	0.3236916993881565	1.3382471357090238
9	0.225863823788902	1.0505191136944885
>10	0.995074374259208	8.894085782785965
>50	0.06965853598893548	2.5203376041735104
>100	0.05344387168924838	5.5499221537713535
>500	0.006570581199991797	2.5289981349654633
>1k	0.0033948002866624285	3.433194057769483
>5k	2.190193733330599E-4	0.933171845222224
>10k+	1.0950968666652995E-4	0.8060060294532666
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	14242	0.7975900929028371	No Hit
TATCAACGCAGAGTACTTTTTTTTT	8427	0.471934539593611	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8062	0.4514935633325848	No Hit
GTCCTACAGTGGACATTTCTAAATT	4109	0.23011499029193636	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3962	0.22188259711283811	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3886	0.21762639383656962	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3669	0.20547381342932938	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3399	0.19035309126363875	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3177	0.1779204974829598	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3164	0.17719246271201913	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2848	0.1594956175106923	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2785	0.1559674490053645	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2172	0.12163780942177797	No Hit
CCCATGTACTCTGCGTTGATACCAC	1951	0.10926121831578675	No Hit
GGATACCACGTGTCCCGCCCTACTC	1857	0.1039969668951389	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.600267468774309E-5	0.0
6	0.0	0.0	0.0	5.600267468774309E-5	0.0
7	0.0	0.0	0.0	5.600267468774309E-5	0.0
8	0.0	0.0	0.0	5.600267468774309E-5	0.0
9	0.0	0.0	0.0	5.600267468774309E-5	0.0
10	0.0	0.0	0.0	5.600267468774309E-5	0.0
11	0.0	0.0	0.0	5.600267468774309E-5	0.0
12	0.0	0.0	0.0	5.600267468774309E-5	0.0
13	0.0	0.0	0.0	5.600267468774309E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCACCG	45	6.769759E-4	14.775837	5
TAGGACC	680	0.0	13.68938	4
TAGAACG	55	0.00307177	12.089322	4
CGTGCGC	130	2.6229827E-9	11.690445	10
GGCGAGG	1090	0.0	11.502432	19
GGTATCA	2680	0.0	11.267679	1
TGTAGGA	2425	0.0	11.241823	2
TTTCGCC	60	0.005881982	11.081878	3
TTTTCGC	60	0.005881982	11.081878	2
TTAGGAC	1545	0.0	11.066508	3
AGGACCT	1275	0.0	11.026003	5
GTAGGAC	2360	0.0	10.907466	3
GTCTTAG	115	8.512543E-7	10.768508	1
CTTAGAC	115	8.8323213E-7	10.7377205	3
GTCCTAA	1095	0.0	10.700424	1
TGTCCCG	330	0.0	10.650117	11
GTCCTAC	2165	0.0	10.647986	1
TTTAGGA	1705	0.0	10.640831	2
TAGAACC	125	2.2442327E-7	10.638602	4
CGAATGG	125	2.2459608E-7	10.638006	19
>>END_MODULE