FastQCFastQC Report
Thu 26 May 2016
SRR1512890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785629
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT186331.043497837456717No Hit
TATCAACGCAGAGTACTTTTTTTTT142130.7959660153368926No Hit
GGTATCAACGCAGAGTACTTTTTTT109580.6136773092282888No Hit
ACGCAGAGTACTTTTTTTTTTTTTT68500.38361832161104015No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41080.2300589876172486No Hit
GTCCTACAGTGGACATTTCTAAATT39310.22014651419751807No Hit
CTTTAGGACGTGAAATATGGCGAGG35160.19690540420210467No Hit
GAGTACTTTTTTTTTTTTTTTTTTT35010.19606536408178854No Hit
CTGTAGGACGTGGAATATGGCAAGA34410.19270520360052396No Hit
GTCCTAAAGTGTGTATTTCTCATTT34330.19225718220302201No Hit
GTACATGGGAAGCAGTGGTATCAAC28030.15697549714974388No Hit
GTACTGGTTCACTATCGGTCAGTCA27650.15484739551160964No Hit
CCCATGTACTCTGCGTTGATACCAC23330.13065424004650464No Hit
GCAGAGTACTTTTTTTTTTTTTTTT22230.12449394583085288No Hit
GTCCTACAGTGTGCATTTCTCATTT21200.11872567033801534No Hit
ATCAACGCAGAGTACTTTTTTTTTT20940.11726960079613404No Hit
GGATACCACGTGTCCCGCCCTACTC18070.10119683316075175No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG456.7224423E-414.7895136
GCGTGCG1155.342372E-912.3886369
CGTGCGC1155.349648E-912.38759510
TAGGACC7250.012.0647454
GGTATCA27700.011.914851
GGCGAGG10550.011.60840919
TGCCGGT1251.8400897E-811.39658710
CGCGCCT2750.011.39339512
GCGCCAC1451.2296368E-911.13147913
CGTCCAA600.00588934311.08001710
GCCGGTT1201.2832243E-711.078464511
TCCAACG1803.6379788E-1211.07629318
TTAGGAC14000.011.0719823
GACGTGA10850.011.0354487
CCAACGT951.3704381E-510.99270319
TGTAGGA20000.010.9361292
CTGTAGG20300.010.7763251
GGACGTG19750.010.7351016
AGGACGT19850.010.7289165
TGGCGAG22400.010.72309518
Produced by FastQC (version 0.11.5)