FastQCFastQC Report
Thu 26 May 2016
SRR1512889_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512889_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289032
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT63090.27561868947223106No Hit
GTATCAACGCAGAGTACTTTTTTTT54990.24023255245011868No Hit
GTCCTAAAGTGTGTATTTCTCATTT53370.23315532504569617No Hit
CTGTAGGACGTGGAATATGGCAAGA50850.22214630463881674No Hit
CTTTAGGACGTGAAATATGGCGAGG44190.19305103642063545No Hit
GTCCTACAGTGTGCATTTCTCATTT33450.14613163992464936No Hit
TATCAACGCAGAGTACTTTTTTTTT30880.134904186573189No Hit
GGTATCAACGCAGAGTACTTTTTTT30450.13302566324979292No Hit
GTACTGGTTCACTATCGGTCAGTCA25100.10965333817963227No Hit
CTGAAGGACCTGGAATATGGCGAGA23490.10261979736412598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9200.014.0409744
ACCGCGT500.00150169513.2979188
ATAGCGA752.0763834E-411.39896317
CTAAGAC1850.011.2952823
ATGCGTT600.005882987811.08184111
AACGCCG600.00588586111.0811145
ACCGTAT951.3632236E-510.9982798
AGGACCT18050.010.9977975
GTCCTAC29450.010.8985791
GCGCCAC1252.2448694E-710.638813
AGCACCG1252.2475615E-710.6378695
GGACCTG16900.010.6225976
GGCGAGG11750.010.58983219
GTCCTAA15000.010.5400361
GGTATCA20900.010.5266691
TCCAACG1909.094947E-1210.49904518
TCGAAAC2001.8189894E-1210.44904916
GTATTAT1105.867738E-610.3899581
TGTAGGA28800.010.322812
TCCTACA32100.010.2972292
Produced by FastQC (version 0.11.5)