##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512889_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2289032
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.77633820759168	31.0	30.0	33.0	22.0	34.0
2	28.914351131832145	31.0	30.0	34.0	22.0	34.0
3	29.025789940900783	31.0	30.0	34.0	22.0	34.0
4	32.496625211006226	35.0	33.0	37.0	25.0	37.0
5	32.65716250362599	35.0	33.0	37.0	25.0	37.0
6	32.6534294846031	36.0	33.0	37.0	25.0	37.0
7	32.64615872560978	36.0	33.0	37.0	25.0	37.0
8	32.57600461679872	36.0	33.0	37.0	25.0	37.0
9	33.90061519454512	39.0	34.0	39.0	23.0	39.0
10	33.74232645065687	38.0	34.0	39.0	22.0	39.0
11	33.82188497146392	38.0	34.0	39.0	23.0	39.0
12	33.706547134334514	38.0	34.0	39.0	22.0	39.0
13	33.68962338665427	38.0	34.0	39.0	22.0	39.0
14	34.53043120410724	39.0	34.0	41.0	18.0	41.0
15	34.70908139335754	39.0	34.0	41.0	21.0	41.0
16	34.65713541794086	39.0	34.0	41.0	19.0	41.0
17	34.408918704500415	39.0	34.0	41.0	17.0	41.0
18	34.412428048188055	39.0	34.0	41.0	17.0	41.0
19	34.52557675034687	39.0	34.0	41.0	18.0	41.0
20	34.49772567618103	39.0	34.0	41.0	18.0	41.0
21	34.391394703088466	39.0	34.0	41.0	18.0	41.0
22	34.4072184224598	39.0	34.0	41.0	18.0	41.0
23	34.06601130958414	39.0	34.0	41.0	10.0	41.0
24	34.135891503482696	39.0	34.0	41.0	10.0	41.0
25	33.8434185280066	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6641727309249781
1101	2	0.3174046030595399
1101	3	0.5973491834850471
1101	4	0.7431110783972912
1101	5	0.5667733236073502
1101	6	0.6397874018582144
1101	7	0.40887534368008716
1101	8	0.9043190666634686
1101	9	0.8359445753575585
1101	10	0.9225066568431473
1101	11	0.9410633723727386
1101	12	0.6778226970585663
1101	13	1.0860106630978166
1101	14	1.2715356178606214
1101	15	1.1979070946227637
1101	16	0.8367203408727093
1101	17	0.33611647306471326
1101	18	0.7198670535236502
1101	19	0.3103668942257869
1101	20	-0.3758955388367937
1101	21	0.7445695833449406
1101	22	0.7279188192273196
1101	23	0.4656879053059626
1101	24	1.007157808180672
1101	25	0.6282660965078151
1102	1	1.1352496447538378
1102	2	1.1792480837016726
1102	3	1.1423379791997021
1102	4	1.4062990732810547
1102	5	1.1229732508045416
1102	6	1.1672208691086396
1102	7	1.1297862804652112
1102	8	1.1238208683659892
1102	9	1.3707962479853677
1102	10	1.446985432152971
1102	11	1.1459024213944105
1102	12	1.3766079254356356
1102	13	1.2850705717937387
1102	14	1.444918844494424
1102	15	1.4644547053214367
1102	16	1.5169395173994857
1102	17	1.6095512961839447
1102	18	1.6640281347455357
1102	19	1.503055722334345
1102	20	1.6931776279851647
1102	21	1.8112292368970841
1102	22	1.6533623987209722
1102	23	1.568299288592705
1102	24	0.946950887906425
1102	25	1.380515803459815
1103	1	0.7572777779401463
1103	2	0.8269614114729364
1103	3	1.0242733074882757
1103	4	1.1530123952179778
1103	5	0.9502646391245833
1103	6	0.5301289568648855
1103	7	0.5178160628248847
1103	8	0.7516638935100488
1103	9	0.9293278030119438
1103	10	1.025889043025849
1103	11	0.8618284186289316
1103	12	0.8821257522607624
1103	13	1.103181140311257
1103	14	1.1803898799146992
1103	15	1.0119146282692597
1103	16	1.153230180316612
1103	17	0.8521212645034808
1103	18	1.1212813859313684
1103	19	1.1404617165350146
1103	20	1.250759644216302
1103	21	1.1195565900321682
1103	22	1.0646454283769202
1103	23	0.46460702667752685
1103	24	0.6218079952018769
1103	25	1.0249244639537523
1104	1	1.0340077994132102
1104	2	0.9727891318260653
1104	3	1.0438314251762542
1104	4	1.1965292070737377
1104	5	1.0906972460610618
1104	6	1.0363777184454506
1104	7	1.2190475209832954
1104	8	1.033331199284305
1104	9	1.3432276565161345
1104	10	1.3572214231622937
1104	11	1.061404136722139
1104	12	1.3131458487493717
1104	13	1.4007491577520028
1104	14	1.723179914778136
1104	15	1.341579389017312
1104	16	1.3117414540319032
1104	17	1.6147398379678322
1104	18	1.3976091735214666
1104	19	1.0371408279806502
1104	20	1.1341308250732922
1104	21	1.2077743446785263
1104	22	0.9774958624711445
1104	23	1.3256438920187463
1104	24	1.2833931625839199
1104	25	0.8757016330350069
1105	1	0.4618759434198658
1105	2	0.6357633798956854
1105	3	0.7458729428818422
1105	4	0.7492121829703322
1105	5	0.5612629093156514
1105	6	0.7427626827716267
1105	7	0.7355087971565553
1105	8	0.7640691754067532
1105	9	0.45696849241189597
1105	10	0.6413464913269706
1105	11	0.5829346300272178
1105	12	0.7857870713036306
1105	13	0.7615721260951815
1105	14	0.8720220116099426
1105	15	0.7789996695181856
1105	16	0.8045305502904441
1105	17	1.055459033002876
1105	18	0.9814644236340442
1105	19	0.7083192864971721
1105	20	0.8586321110631943
1105	21	0.6629425897854588
1105	22	0.7011007042941557
1105	23	0.9405154117780086
1105	24	0.9479791474128234
1105	25	1.1008416063884994
1106	1	0.818897624628999
1106	2	0.8450073170560373
1106	3	0.8085984396625143
1106	4	0.919833837870847
1106	5	0.8032168047300488
1106	6	0.8938068692314189
1106	7	0.8406020757477322
1106	8	0.7614705671072741
1106	9	1.0585820453968822
1106	10	0.8640018149324433
1106	11	0.7802845112273715
1106	12	0.9950989934705987
1106	13	0.8484851071839685
1106	14	1.2600385316503377
1106	15	0.9029461390726041
1106	16	1.0896876525734172
1106	17	1.3343834912256156
1106	18	1.111528595156102
1106	19	1.1440089485389606
1106	20	1.2103215542881287
1106	21	1.2761126452675455
1106	22	1.2220732883125578
1106	23	1.4605240316444252
1106	24	1.1988996327815045
1106	25	1.3134408407824623
1107	1	1.0513403036170708
1107	2	1.0627090450414123
1107	3	0.9479210971724541
1107	4	1.1888451492337353
1107	5	0.9857511874165894
1107	6	1.0947060181045245
1107	7	0.9196648937112357
1107	8	0.9913231901492665
1107	9	1.03736060669668
1107	10	1.170173740455894
1107	11	1.1192014465257287
1107	12	1.1608656742121397
1107	13	0.962157700798933
1107	14	1.2714858379859564
1107	15	1.1441107450412176
1107	16	1.1840018682587754
1107	17	1.4789488845191556
1107	18	1.3174858633407496
1107	19	1.2096266960257864
1107	20	1.339388309496293
1107	21	1.153515217063358
1107	22	1.2229149906159407
1107	23	1.4646753446070164
1107	24	1.327992429607896
1107	25	1.6074460602667955
1108	1	0.9405800578224905
1108	2	1.0005628383234892
1108	3	0.9948424471736672
1108	4	1.1610424883477037
1108	5	0.9737980240732682
1108	6	1.0355112742411166
1108	7	0.9982400592164069
1108	8	1.0090315257820492
1108	9	1.0503932235583804
1108	10	1.2612170723838076
1108	11	0.9004983506942494
1108	12	0.990703550153718
1108	13	1.016819770038481
1108	14	1.1108405646605277
1108	15	1.0790229355077372
1108	16	0.860506719913019
1108	17	1.1582218317363058
1108	18	1.2578511704257807
1108	19	1.0803760074786624
1108	20	1.1745646607079436
1108	21	1.1472345194140914
1108	22	1.1455930066289355
1108	23	1.4224713610041135
1108	24	1.2452551503458409
1108	25	1.3570095327959208
1109	1	0.8020971759529729
1109	2	0.7990557644154848
1109	3	0.9042901103867749
1109	4	0.9420951786647933
1109	5	0.8684817815816572
1109	6	0.9476663987402816
1109	7	0.8321586202493734
1109	8	0.7353539967195175
1109	9	0.9729394926950832
1109	10	0.9118072805884978
1109	11	0.8637537507487281
1109	12	0.8898562524387046
1109	13	1.0076846671696416
1109	14	1.1273207254855606
1109	15	0.9254934706195641
1109	16	0.6262871993190586
1109	17	1.103989711810641
1109	18	1.1794468361381902
1109	19	1.0072608227141657
1109	20	0.8687970890088579
1109	21	0.9958014922778062
1109	22	0.9630024783013624
1109	23	1.319203611255034
1109	24	1.072531161614755
1109	25	0.6941349027544419
1110	1	0.7006386466994066
1110	2	0.7590803871010756
1110	3	0.7297053239579725
1110	4	0.6313884004819954
1110	5	0.5127119283644035
1110	6	0.5774920684785059
1110	7	0.5919303072211264
1110	8	0.5828014822513339
1110	9	0.7574472979216367
1110	10	0.6359618467902095
1110	11	0.6112092398303517
1110	12	0.7170815335339711
1110	13	0.6805729658621189
1110	14	0.676314808743669
1110	15	0.592991143116663
1110	16	0.6906666358456093
1110	17	0.7056640720857246
1110	18	-0.08171826297046891
1110	19	0.4700928670444142
1110	20	0.5066201293455705
1110	21	0.6856692336171974
1110	22	0.6859581303964717
1110	23	0.7154180204694711
1110	24	0.69552556619184
1110	25	0.9210764403540637
1111	1	0.29808312501825895
1111	2	0.3272439189546894
1111	3	0.3454497355097814
1111	4	0.22391085755593565
1111	5	0.22339632272414178
1111	6	0.09532467630786101
1111	7	0.10297027557553662
1111	8	0.28824216228038324
1111	9	0.30387562692574477
1111	10	0.31891853540242465
1111	11	0.0751015681743965
1111	12	-0.25541707231636934
1111	13	0.17639966003922325
1111	14	0.20211081992495394
1111	15	0.2321241341689415
1111	16	0.3406193547154075
1111	17	-0.16165961991515587
1111	18	0.10408743557604794
1111	19	0.1336994751675249
1111	20	0.23878883813184615
1111	21	0.3742580381711704
1111	22	0.11089421602213179
1111	23	0.45975035590568325
1111	24	0.5038328402883963
1111	25	0.4753823001128481
1112	1	0.2479364030090565
1112	2	0.19893083897816055
1112	3	0.19240613869539658
1112	4	0.20748951412826244
1112	5	0.21062190901497502
1112	6	-0.004500547817180234
1112	7	-0.15560006388497527
1112	8	0.06350651825626841
1112	9	0.025688701949874826
1112	10	0.21800014272870527
1112	11	0.014548187465088347
1112	12	0.03976487287983588
1112	13	0.0545281904419852
1112	14	0.035855177835180996
1112	15	-0.12240535618291659
1112	16	0.108976793592646
1112	17	0.34852949751546447
1112	18	0.03862826982751244
1112	19	0.15556568648566582
1112	20	-0.029523009572073988
1112	21	-0.07463855347349835
1112	22	-0.27097592225458556
1112	23	-0.009589944186195964
1112	24	0.035390307598213155
1112	25	-0.062440821865827445
1113	1	-0.2642228577024355
1113	2	-0.049569116235531396
1113	3	-0.01907007146925821
1113	4	-0.03979209032497977
1113	5	-0.1858213164772735
1113	6	-0.5788192027092975
1113	7	-0.41072920862068685
1113	8	-0.42454502811714434
1113	9	-0.5137283671509536
1113	10	-0.3167963731929788
1113	11	-0.4940755499910381
1113	12	-0.6009852266288149
1113	13	-0.6052708465633714
1113	14	-0.216686148658205
1113	15	-0.466282652371099
1113	16	-0.6006300330273859
1113	17	-0.4686382518330561
1113	18	-0.5850464558044308
1113	19	-0.9921357822086989
1113	20	-0.7766453408567102
1113	21	-0.5407887786210992
1113	22	-1.0412161459369926
1113	23	-4.555246176334698
1113	24	-2.20873104431956
1113	25	-1.4716461436658435
1114	1	-0.6205723096195968
1114	2	-0.8289462173935789
1114	3	-0.7282724049497666
1114	4	-0.5926882553095609
1114	5	-0.5960412449292534
1114	6	-1.3448227696803983
1114	7	-1.2098402471099021
1114	8	-0.9354067332405762
1114	9	-1.4093397063643422
1114	10	-1.0541346687648598
1114	11	-1.2060574091965037
1114	12	-2.032387087989761
1114	13	-1.22484260143667
1114	14	-3.179488480748084
1114	15	-1.8483266380614936
1114	16	-1.9077441587196589
1114	17	-1.735877743000806
1114	18	-1.6633112251854953
1114	19	-1.732405703881355
1114	20	-1.259969924796998
1114	21	-1.2845161591223544
1114	22	-1.3572928949174923
1114	23	-5.883059859458335
1114	24	-3.02078104449339
1114	25	-2.9085236185562486
1115	1	-1.7609333897536459
1115	2	-1.7399948750077812
1115	3	-1.5417866743200825
1115	4	-1.655219799239834
1115	5	-1.518491613959494
1115	6	-1.7418471824987733
1115	7	-2.3300540264197522
1115	8	-2.9173286665988343
1115	9	-4.001209209700491
1115	10	-4.571075486396236
1115	11	-3.3450232510704545
1115	12	-3.4580364760732465
1115	13	-2.904343882568881
1115	14	-3.5645756698388773
1115	15	-3.153181552657447
1115	16	-3.218893010496778
1115	17	-3.0717524305789183
1115	18	-5.700743091090164
1115	19	-3.745464613094228
1115	20	-3.9989032261986353
1115	21	-3.6607856583929355
1115	22	-3.134072877810315
1115	23	-3.5378265701581313
1115	24	-3.2924994672921137
1115	25	-3.632183702275306
1116	1	-1.2225071483458478
1116	2	-1.0638948799701993
1116	3	-1.552088966796049
1116	4	-1.291744330947914
1116	5	-1.2243002889835672
1116	6	-3.760144079883382
1116	7	-2.201435797731321
1116	8	-2.5811771596934463
1116	9	-3.5497793029129063
1116	10	-2.836479341450751
1116	11	-2.451708636548336
1116	12	-2.270180167004632
1116	13	-3.519723801243572
1116	14	-6.784934047393431
1116	15	-4.569844273172965
1116	16	-3.4949883674553384
1116	17	-4.500440587227928
1116	18	-3.1284436288154254
1116	19	-2.8154083042202203
1116	20	-3.5098996088213426
1116	21	-4.299951868008808
1116	22	-3.84948951190772
1116	23	-2.984457524697497
1116	24	-3.946585858162816
1116	25	-3.7393007858771696
1201	1	0.4335974054875571
1201	2	0.511994123860184
1201	3	0.8122326373852573
1201	4	0.62252551543056
1201	5	0.24696713383962532
1201	6	0.7769304114208637
1201	7	0.7618377493385893
1201	8	0.758171688580255
1201	9	1.1265347701923858
1201	10	1.001302523557115
1201	11	1.1678998403319838
1201	12	0.9456126693605071
1201	13	1.0206490767803729
1201	14	1.1247483306873391
1201	15	1.1846084678404054
1201	16	1.2289852905022016
1201	17	1.4038829896543348
1201	18	1.2055843876337633
1201	19	1.3362365973205002
1201	20	1.2641204144346858
1201	21	1.3606821335058754
1201	22	1.095112830145652
1201	23	1.552573621793293
1201	24	1.4144397483830033
1201	25	1.5280914911692278
1202	1	0.7782085017890985
1202	2	0.7271540605724844
1202	3	0.8375238443474373
1202	4	0.974823606852901
1202	5	0.7095802115288592
1202	6	1.1027653229679473
1202	7	1.0960124098568471
1202	8	1.0986086415932448
1202	9	1.3969989971262606
1202	10	1.1640716487890401
1202	11	1.2538673980926163
1202	12	1.4065736788602905
1202	13	1.357942615918084
1202	14	1.8228668744337
1202	15	1.4632905963600535
1202	16	1.2512147296262555
1202	17	1.6109164517646093
1202	18	1.6703419793060945
1202	19	1.6005437183801021
1202	20	1.480522282694949
1202	21	1.6219051065953352
1202	22	1.7096632978406774
1202	23	1.8241335634126514
1202	24	1.889338772542935
1202	25	2.1255288665864924
1203	1	1.0163792977793484
1203	2	1.063988003469543
1203	3	1.0376152464478672
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2211	14	-0.061770537531323555
2211	15	-0.4113408176171873
2211	16	-0.346732410687963
2211	17	0.11636881613269168
2211	18	-0.3448716264519689
2211	19	-0.22903700292629736
2211	20	-0.17675946794354047
2211	21	-0.05192528578915301
2211	22	-0.13078635775872982
2211	23	0.06710527612838035
2211	24	-0.2351088321316226
2211	25	0.21585566017832036
2212	1	0.16340256571603717
2212	2	0.16668221886776635
2212	3	-0.12477828655058687
2212	4	0.2176850984815175
2212	5	-0.06307199547266862
2212	6	-0.17379860397269198
2212	7	-0.09968393717511503
2212	8	-0.009538238163223411
2212	9	0.09866566379292863
2212	10	-0.2482837545595089
2212	11	-0.24804660495151865
2212	12	0.08577459210229677
2212	13	0.14342967832295273
2212	14	0.21852136454024418
2212	15	-0.17602066695805263
2212	16	-0.11497629016065503
2212	17	0.15928783684832126
2212	18	0.39961519087383124
2212	19	0.01927278991739456
2212	20	0.20392791435401136
2212	21	0.37886096656490054
2212	22	0.4165582750096277
2212	23	0.47357420268205175
2212	24	0.4203243128777885
2212	25	0.5071080142272848
2213	1	-0.5071319297645012
2213	2	-0.3621365722531813
2213	3	-0.549701886132457
2213	4	-0.3741977029696315
2213	5	-0.4273533396281124
2213	6	-0.2871688308680973
2213	7	-0.4097803216060285
2213	8	-0.2469115702667679
2213	9	-0.6622382284778681
2213	10	-0.3204985980693209
2213	11	-0.49517551720633435
2213	12	-0.48730094746604635
2213	13	-0.44279503421471134
2213	14	-0.7445238915625652
2213	15	-0.48134963455321156
2213	16	-0.3763494992856593
2213	17	-0.5623360947756098
2213	18	-0.4997540829378906
2213	19	-0.4917111921277382
2213	20	-0.4097002600295596
2213	21	-0.554104507209928
2213	22	-0.6483356559994107
2213	23	-0.49197366671473475
2213	24	-0.37675525423775014
2213	25	-0.18565869548186953
2214	1	-2.675094047733406
2214	2	-1.3553077827448767
2214	3	-1.6091562094662457
2214	4	-3.5186203361258066
2214	5	-1.7524047643809055
2214	6	-1.4038711729131812
2214	7	-1.2311437823613716
2214	8	-1.0057352225205847
2214	9	-0.8966995998742178
2214	10	-0.8365775781363567
2214	11	-0.855320028592871
2214	12	-0.8559271495522722
2214	13	-1.2447831099370035
2214	14	-1.2056739265418415
2214	15	-1.5021221022104356
2214	16	-1.3819795960790557
2214	17	-3.45667571741712
2214	18	-3.14223249498329
2214	19	-1.9307397080668025
2214	20	-1.7769533951122085
2214	21	-1.6333664967474704
2214	22	-2.7826821700798696
2214	23	-1.5090310222816612
2214	24	-2.688631500700666
2214	25	-2.6747263673240766
2215	1	-1.7605609615231117
2215	2	-3.5911632720525155
2215	3	-2.0951530172369495
2215	4	-3.6230874469170686
2215	5	-2.1567231188952576
2215	6	-1.9637709031416648
2215	7	-2.208684398855631
2215	8	-1.613587945765559
2215	9	-4.179155204166442
2215	10	-2.438308377520425
2215	11	-1.8607343550284696
2215	12	-1.8576949369835738
2215	13	-2.0500044552566123
2215	14	-1.702658998648431
2215	15	-1.8719563394726748
2215	16	-1.977976444054157
2215	17	-2.2059968661372125
2215	18	-1.8785250061098537
2215	19	-2.034881565268819
2215	20	-1.8190345356305997
2215	21	-2.490299544361541
2215	22	-2.4915396012599906
2215	23	-2.5525615313899657
2215	24	-2.377864360560192
2215	25	-3.4040631153510468
2216	1	-3.0446810361457572
2216	2	-3.2890630968023586
2216	3	-4.472322444564668
2216	4	-5.090086711997063
2216	5	-4.6590437701474485
2216	6	-3.7891369498373493
2216	7	-5.475112687622662
2216	8	-3.2986577631662044
2216	9	-4.0802208381762775
2216	10	-3.1325109893130616
2216	11	-3.3455102044264002
2216	12	-3.4983473198203647
2216	13	-3.2244235857897117
2216	14	-4.223884053767431
2216	15	-3.6795288421579606
2216	16	-4.053305744749508
2216	17	-3.83357049946866
2216	18	-3.4691462961771293
2216	19	-4.087108938212452
2216	20	-4.736053587018471
2216	21	-4.374119997750654
2216	22	-3.891737464338739
2216	23	-3.93118949226961
2216	24	-4.26058808716995
2216	25	-4.446776100822245
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	110030.0
3	2.0
4	942.0
5	5882.0
6	6905.0
7	6344.0
8	5839.0
9	5311.0
10	4823.0
11	4558.0
12	4532.0
13	4275.0
14	4408.0
15	4220.0
16	4599.0
17	4879.0
18	5598.0
19	6796.0
20	8561.0
21	11178.0
22	14053.0
23	16924.0
24	19999.0
25	22649.0
26	25240.0
27	27857.0
28	31311.0
29	37573.0
30	45652.0
31	56060.0
32	70759.0
33	90099.0
34	116608.0
35	153041.0
36	207563.0
37	324922.0
38	819040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.67104109685111	18.233440283329827	12.382574163686092	24.712944456132966
2	15.017600764760546	19.720415008425736	41.91188389131347	23.350100335500244
3	19.68890819025516	25.107630845641594	28.47570955094323	26.727751413160018
4	12.390187849938506	16.130540306143626	37.652651285938674	33.8266205579792
5	13.281060897998845	38.68658297134978	34.61375458869318	13.418601541958196
6	32.24002574340194	35.81415257512719	18.46293352361065	13.48288815786022
7	29.110890657087555	31.259457528619066	21.387994660718196	18.24165715357518
8	25.930997264975503	36.47514206471171	20.29240359410382	17.30145707620896
9	28.060320798564124	14.564525896274985	19.57360943201121	37.801543873149676
10	16.485363076508687	28.152399958722423	33.30378896664387	22.05844799812502
11	35.01862890718427	21.942452177205706	24.425971245764718	18.612947669845305
12	24.829510893592495	24.175060116678175	30.77300493308781	20.222424056641522
13	29.81867024848892	21.81689157991941	25.315551894448173	23.048886277143495
14	21.672286077954084	22.12440419009424	26.995211658175972	29.208098073775705
15	23.652594201333336	31.083936769006964	22.773380299364725	22.49008873029497
16	22.33731038255141	27.482259812797246	27.88443922469184	22.2959905799595
17	21.998566523916704	28.45791120931943	27.296874296217034	22.24664797054683
18	21.81151086058136	26.668838637168683	30.042244097415065	21.477406404834895
19	24.160654736969146	26.135911444690972	27.171488017099566	22.53194580124031
20	24.764931131151002	26.30829823825705	27.70329745771865	21.223473172873295
21	23.95403480881212	26.054228851805895	26.466143913617202	23.52559242576478
22	24.246289830532195	27.87275745532403	26.316314250129448	21.56463846401433
23	22.775133426183235	27.527357560306886	27.462464662012508	22.23504435149737
24	23.905765865509487	26.192006618445067	28.066081256105313	21.83614625994013
25	23.164060928621026	26.88941514750302	27.746038048031394	22.200485875844556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	310.0
1	310.0
2	687.5
3	1065.0
4	1065.0
5	1065.0
6	1685.5
7	2306.0
8	2306.0
9	2306.0
10	2755.5
11	3205.0
12	3205.0
13	3205.0
14	4718.0
15	6231.0
16	6231.0
17	6231.0
18	12636.5
19	19042.0
20	19042.0
21	19042.0
22	33222.0
23	47402.0
24	47402.0
25	47402.0
26	72654.0
27	97906.0
28	97906.0
29	97906.0
30	129947.0
31	161988.0
32	161988.0
33	161988.0
34	185027.0
35	208066.0
36	208066.0
37	208066.0
38	224601.0
39	241136.0
40	241136.0
41	241136.0
42	257682.0
43	274228.0
44	274228.0
45	274228.0
46	293487.5
47	312747.0
48	312747.0
49	312747.0
50	301814.0
51	290881.0
52	290881.0
53	290881.0
54	259702.0
55	228523.0
56	228523.0
57	228523.0
58	197797.5
59	167072.0
60	167072.0
61	167072.0
62	139746.0
63	112420.0
64	112420.0
65	112420.0
66	87512.5
67	62605.0
68	62605.0
69	62605.0
70	46736.0
71	30867.0
72	30867.0
73	30867.0
74	22067.0
75	13267.0
76	13267.0
77	13267.0
78	9245.0
79	5223.0
80	5223.0
81	5223.0
82	3526.0
83	1829.0
84	1829.0
85	1829.0
86	1181.5
87	534.0
88	534.0
89	534.0
90	339.0
91	144.0
92	144.0
93	144.0
94	85.0
95	26.0
96	26.0
97	26.0
98	17.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3316685830517005
2	0.0092178702613157
3	0.00830045189407575
4	0.010353721573136592
5	0.01109639358471179
6	0.08055806996145096
7	0.08007751748337288
8	0.1047604402210192
9	0.08418405684149458
10	0.0906933585900066
11	0.08715474488779537
12	0.09737740669418339
13	0.08422774343041076
14	0.08820322302178388
15	0.08689262535429824
16	0.08706737170996298
17	0.09973648249565754
18	0.1014402594633889
19	0.09567362972645205
20	0.09388247958088834
21	0.08344138482991936
22	0.10240136441954503
23	0.09554256995970349
24	0.09130497083483324
25	0.08859640232202957
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2289032.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.940991771686164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.51042799084404	40.93676811149776
2	14.104445798844953	15.49824481157068
3	5.080720146884254	8.374194113526238
4	2.2552121695527574	4.956143730032182
5	1.253625275322378	3.443770796813229
6	0.7502210636314407	2.4730733570352372
7	0.4722863234289873	1.8163515306574334
8	0.31044126007373957	1.3644760572242567
9	0.22865803123581213	1.130642911139104
>10	0.9361042654227917	8.676705155105704
>50	0.05221648669166588	2.0013442156627264
>100	0.037945911335303215	4.076576761952373
>500	0.0048095479574108806	1.8776108443143862
>1k	0.0025650922439521195	2.3950834582066762
>5k	3.2063653049401494E-4	0.9790141452621068
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	6309	0.27561868947223106	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5499	0.24023255245011868	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5337	0.23315532504569617	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5085	0.22214630463881674	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4419	0.19305103642063545	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3345	0.14613163992464936	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3088	0.134904186573189	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3045	0.13302566324979292	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2510	0.10965333817963227	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2349	0.10261979736412598	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	4.3686588916188156E-5	0.0
7	0.0	0.0	0.0	4.3686588916188156E-5	0.0
8	4.3686588916188156E-5	0.0	0.0	4.3686588916188156E-5	0.0
9	4.3686588916188156E-5	0.0	0.0	4.3686588916188156E-5	0.0
10	4.3686588916188156E-5	0.0	0.0	4.3686588916188156E-5	0.0
11	4.3686588916188156E-5	0.0	0.0	8.737317783237631E-5	0.0
12	4.3686588916188156E-5	0.0	0.0	8.737317783237631E-5	0.0
13	4.3686588916188156E-5	0.0	0.0	8.737317783237631E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	920	0.0	14.040974	4
ACCGCGT	50	0.001501695	13.297918	8
ATAGCGA	75	2.0763834E-4	11.398963	17
CTAAGAC	185	0.0	11.295282	3
ATGCGTT	60	0.0058829878	11.081841	11
AACGCCG	60	0.005885861	11.081114	5
ACCGTAT	95	1.3632236E-5	10.998279	8
AGGACCT	1805	0.0	10.997797	5
GTCCTAC	2945	0.0	10.898579	1
GCGCCAC	125	2.2448694E-7	10.6388	13
AGCACCG	125	2.2475615E-7	10.637869	5
GGACCTG	1690	0.0	10.622597	6
GGCGAGG	1175	0.0	10.589832	19
GTCCTAA	1500	0.0	10.540036	1
GGTATCA	2090	0.0	10.526669	1
TCCAACG	190	9.094947E-12	10.499045	18
TCGAAAC	200	1.8189894E-12	10.449049	16
GTATTAT	110	5.867738E-6	10.389958	1
TGTAGGA	2880	0.0	10.32281	2
TCCTACA	3210	0.0	10.297229	2
>>END_MODULE