FastQCFastQC Report
Thu 26 May 2016
SRR1512889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289032
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72210.3154608585637947No Hit
GTCCTACAGTGGACATTTCTAAATT57220.24997466177842861No Hit
TATCAACGCAGAGTACTTTTTTTTT52130.22773818802008886No Hit
CTGTAGGACGTGGAATATGGCAAGA49370.2156806894792209No Hit
GTCCTAAAGTGTGTATTTCTCATTT48910.21367110638907624No Hit
CTTTAGGACGTGAAATATGGCGAGG47870.2091277011417927No Hit
GGTATCAACGCAGAGTACTTTTTTT42010.18352736003690642No Hit
GTCCTACAGTGTGCATTTCTCATTT31210.13634584400742322No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24690.10786218803406854No Hit
CTGAAGGACCTGGAATATGGCGAGA24590.10742532214490666No Hit
GTACTGGTTCACTATCGGTCAGTCA22920.10012966179590325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8850.013.2154474
ATACCGT550.00304929612.101426
TCCAACG1603.6379788E-1211.86663118
GTCCTAG2700.011.2720071
CTAACCG600.00590801611.07552218
GATATAC4050.010.80234051
AGGACCT18300.010.7037555
TGGACCG803.740456E-410.697265
GGTATCA20450.010.6966941
GGCGAGG12000.010.36329719
TGTAGGA28700.010.3383142
GTAGGAC28650.010.3229383
GGACCTG18450.010.2555216
GTCCTAA15250.010.1655951
CTGTAGG29050.010.1491721
GCGTGCG1502.4563633E-810.1306189
GTCCTAC27650.010.1127031
GTCTAGA1701.5297701E-910.0702121
GCACCGT856.542995E-410.0675696
CTGTTCG856.6117523E-410.0561289
Produced by FastQC (version 0.11.5)