##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512889_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2289032
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.218612059595497	31.0	30.0	34.0	26.0	34.0
2	30.212279251666207	31.0	30.0	34.0	26.0	34.0
3	30.39748592418105	31.0	30.0	34.0	26.0	34.0
4	34.0098836538764	37.0	35.0	37.0	30.0	37.0
5	33.58671263660796	37.0	35.0	37.0	28.0	37.0
6	33.46167113434849	37.0	35.0	37.0	28.0	37.0
7	33.30386294293832	37.0	35.0	37.0	27.0	37.0
8	33.243158680175725	37.0	35.0	37.0	27.0	37.0
9	34.560007898535275	39.0	35.0	39.0	27.0	39.0
10	34.474123122787276	39.0	35.0	39.0	27.0	39.0
11	34.46334957309465	39.0	35.0	39.0	27.0	39.0
12	34.31669937335957	39.0	35.0	39.0	26.0	39.0
13	34.311801669876175	39.0	35.0	39.0	26.0	39.0
14	35.36970212736213	39.0	35.0	41.0	26.0	41.0
15	35.3681232940387	39.0	35.0	41.0	26.0	41.0
16	35.36489791317902	39.0	36.0	41.0	26.0	41.0
17	35.35011480835567	39.0	36.0	41.0	26.0	41.0
18	35.26391068364269	39.0	35.0	41.0	25.0	41.0
19	35.270247860230874	40.0	35.0	41.0	25.0	41.0
20	35.244814401895646	40.0	35.0	41.0	25.0	41.0
21	35.20678828430533	40.0	35.0	41.0	25.0	41.0
22	35.16802342649644	40.0	35.0	41.0	25.0	41.0
23	34.99896550157447	39.0	35.0	41.0	24.0	41.0
24	34.95787345917401	39.0	35.0	41.0	24.0	41.0
25	34.88944933928403	39.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5121239715539261
1101	2	0.4143825326936117
1101	3	0.40352213541489235
1101	4	0.3859883779278661
1101	5	0.5138638482351041
1101	6	0.6350511146331854
1101	7	0.6639821250802243
1101	8	0.5268544150594465
1101	9	0.759988512031839
1101	10	0.5228423622363607
1101	11	0.8367132498730143
1101	12	0.8826239138421386
1101	13	0.9338869458729917
1101	14	1.0676590647087778
1101	15	0.7002794098034357
1101	16	0.9879966793538628
1101	17	1.002695708244076
1101	18	0.9270593358935741
1101	19	0.7636875102379079
1101	20	0.8869038882878044
1101	21	0.9384437848259921
1101	22	0.8334722425367218
1101	23	0.9271899928719805
1101	24	1.0103565587221937
1101	25	0.9934445742868192
1102	1	0.7760413303482032
1102	2	0.9251226166506115
1102	3	0.6064237280598057
1102	4	0.6579304095269549
1102	5	0.8520046715747469
1102	6	0.8250512482163188
1102	7	0.8761176518486167
1102	8	0.9222873746939655
1102	9	0.9111331525095352
1102	10	0.8915318115064892
1102	11	0.9507525032768811
1102	12	0.9749926433663489
1102	13	1.1043772627655812
1102	14	1.019638933730512
1102	15	1.229305855924352
1102	16	1.2189104881763626
1102	17	1.218463026462473
1102	18	0.584323419730346
1102	19	1.0511602509296694
1102	20	1.255028781838746
1102	21	1.2779421467628111
1102	22	0.9674738254968602
1102	23	1.148130651904367
1102	24	1.0684463533047293
1102	25	1.1094553477665556
1103	1	0.7204337519298143
1103	2	0.9352510304686632
1103	3	0.6291431332683146
1103	4	0.5615485753272225
1103	5	0.7020081721786013
1103	6	0.6903387742034397
1103	7	0.8010233786420429
1103	8	0.8869444030313502
1103	9	0.8687700115343802
1103	10	0.888553381060575
1103	11	1.0177628125552332
1103	12	1.0010283845317005
1103	13	1.1192504533909613
1103	14	1.1468557397406585
1103	15	1.1817564731002577
1103	16	1.0926962162144491
1103	17	1.1006555518953363
1103	18	1.1487549508361283
1103	19	1.0711929426801063
1103	20	1.0060468741263264
1103	21	1.1037273030356047
1103	22	1.041794562490658
1103	23	1.0211097824710578
1103	24	1.0503749721025812
1103	25	0.9145674935005843
1104	1	0.7813597119098006
1104	2	0.8646778721320132
1104	3	0.7661302613088878
1104	4	0.5551643894623837
1104	5	0.6715367578691485
1104	6	0.6027691554299324
1104	7	0.5780019155560936
1104	8	0.6188337808731816
1104	9	0.7012413811775815
1104	10	0.7544282195611771
1104	11	0.8684485108479834
1104	12	0.9489496692852555
1104	13	0.8559699640388772
1104	14	1.0443835100344643
1104	15	1.021855793745523
1104	16	1.0618173411198342
1104	17	1.002698890517344
1104	18	0.4718114816841208
1104	19	0.9917159940315656
1104	20	0.8175141748637529
1104	21	0.7932307805886722
1104	22	0.7815663464371312
1104	23	0.7878476300422363
1104	24	0.9829172850638273
1104	25	0.9229412284120642
1105	1	0.35579624436131496
1105	2	0.35666855097542083
1105	3	0.41509646326821326
1105	4	0.18104051810887256
1105	5	0.21201319116694606
1105	6	0.3260256646623745
1105	7	0.45217025985398607
1105	8	0.38666661662179536
1105	9	0.5757985726855281
1105	10	0.26123351142243934
1105	11	0.4631316422834644
1105	12	0.5564519523073486
1105	13	0.5977088235663075
1105	14	0.6673728556209113
1105	15	0.7194115979770217
1105	16	0.6409933855238563
1105	17	0.42560585637748716
1105	18	0.35705615040259175
1105	19	0.5485105052785642
1105	20	0.7080380847759997
1105	21	0.6999835897187126
1105	22	0.5450564340930697
1105	23	0.8703419149196208
1105	24	0.7375921831835299
1105	25	0.614449410735304
1106	1	0.12746749290121784
1106	2	0.4629321203084267
1106	3	-1.427290733795342
1106	4	-0.2362829153426631
1106	5	0.13043355479503305
1106	6	0.18366569322240167
1106	7	0.3207603014102176
1106	8	0.4242798748996819
1106	9	0.5933195737283867
1106	10	0.40113969843749686
1106	11	0.42654274414017834
1106	12	0.6166835060777984
1106	13	0.6542563805013799
1106	14	0.6842242834009085
1106	15	0.6264210735058953
1106	16	0.594754410952774
1106	17	0.6744866334429531
1106	18	0.7240531293386283
1106	19	0.7904957054169373
1106	20	0.8119433649026888
1106	21	0.7440549493113338
1106	22	0.6717442804608709
1106	23	0.7628326389188302
1106	24	0.7050740058900118
1106	25	0.6740849099128425
1107	1	0.4978220867356775
1107	2	0.6677858749207388
1107	3	0.5280815280370099
1107	4	0.38204229238574783
1107	5	0.49623226052661096
1107	6	0.3729309041626081
1107	7	0.3880465725791069
1107	8	0.38368083707546674
1107	9	0.5115350966409835
1107	10	0.4972453604433298
1107	11	0.5471265972208101
1107	12	0.7616903668041246
1107	13	0.5373150987701294
1107	14	0.6444004487478239
1107	15	0.6060546679374497
1107	16	0.7213854998086404
1107	17	0.6747560401838868
1107	18	0.726043763036607
1107	19	0.6925671301352736
1107	20	0.7403482909847909
1107	21	0.740268994052073
1107	22	0.7366082161058287
1107	23	1.0498379334278312
1107	24	0.9082353653368926
1107	25	0.9881260544295642
1108	1	0.5377464146158211
1108	2	0.561659405940496
1108	3	0.4625996848572491
1108	4	0.40074553538199353
1108	5	0.574533258082937
1108	6	0.5662716987492971
1108	7	0.45595023264028356
1108	8	0.44456253058866935
1108	9	0.7411453822804219
1108	10	0.7240420766088675
1108	11	0.7331890642467087
1108	12	0.7414923713353261
1108	13	0.7327621889916927
1108	14	0.8304166324565401
1108	15	0.8055284067538508
1108	16	0.7961682294353309
1108	17	0.8873120639686576
1108	18	0.9438782188691448
1108	19	0.9007800275077145
1108	20	1.0033752052120235
1108	21	0.94616125031596
1108	22	0.9889368409026531
1108	23	1.014352536219107
1108	24	0.9312310364854568
1108	25	0.9748014626830042
1109	1	0.3755017511596819
1109	2	0.32320545260648004
1109	3	0.38686925769144764
1109	4	0.33477070614478777
1109	5	-0.10653213435760733
1109	6	0.30052903202291503
1109	7	0.22643717918930406
1109	8	0.29518482450405514
1109	9	0.5579951528712641
1109	10	0.13994924684742216
1109	11	0.3148328071292994
1109	12	0.6233919922540352
1109	13	0.4711253184718558
1109	14	0.6291222640494354
1109	15	0.48641986086798283
1109	16	0.6112960913595771
1109	17	0.6139082546532251
1109	18	0.7618419583449239
1109	19	0.5906037910514499
1109	20	0.629915741505954
1109	21	0.7333138233770313
1109	22	0.7796460085317705
1109	23	0.7604927426983679
1109	24	0.7107113801329632
1109	25	0.5849331315731519
1110	1	0.2707773616363305
1110	2	0.007833517053889238
1110	3	0.16246722879157005
1110	4	0.16293875832793248
1110	5	0.24596464951005714
1110	6	0.2990177735000188
1110	7	0.23373114957381347
1110	8	0.30382851117450116
1110	9	0.2729409288213063
1110	10	0.37495906885082775
1110	11	0.3200946997569716
1110	12	0.37856678742789285
1110	13	0.409950832847052
1110	14	0.3335560246703224
1110	15	0.5024843388776361
1110	16	0.3108684310914356
1110	17	0.31999568990391936
1110	18	0.3179472838834272
1110	19	0.36398901602770195
1110	20	0.4423363680662007
1110	21	0.45432920313958647
1110	22	0.5204467813451217
1110	23	0.6025337126344397
1110	24	0.575848770481393
1110	25	0.4805443320703162
1111	1	-0.13376376270660728
1111	2	-0.26229841368393636
1111	3	-0.22244937384856556
1111	4	0.01712291891524842
1111	5	0.03821711260532368
1111	6	0.15040171207667896
1111	7	-0.0634328749866242
1111	8	-0.055713982448693855
1111	9	0.023347251034010696
1111	10	0.045673645892442494
1111	11	0.08641671058598632
1111	12	0.14130102066313555
1111	13	0.17225270413822358
1111	14	-0.02140567059225873
1111	15	0.07573220142531056
1111	16	-0.022575156610088243
1111	17	0.21534603254582407
1111	18	0.03352327103794295
1111	19	-0.16268203253083868
1111	20	0.11418235680650923
1111	21	0.32411489322464604
1111	22	0.05419932021892748
1111	23	0.09059647072768229
1111	24	0.2093842433248767
1111	25	0.04531838327320514
1112	1	0.009947772472010286
1112	2	-0.4208078532416941
1112	3	-0.04222149372451156
1112	4	0.04118376987668171
1112	5	-0.10759227200997401
1112	6	-0.1007025265332473
1112	7	0.04125938896325465
1112	8	-0.0172068472115825
1112	9	-0.03420781815095353
1112	10	-0.453072888158772
1112	11	-0.3162004703821353
1112	12	0.0952499299999161
1112	13	0.06656173130706122
1112	14	-0.17607066723336828
1112	15	-0.5056934884579931
1112	16	-0.5460459441750274
1112	17	-0.3144248579706854
1112	18	-0.11066129077116216
1112	19	-0.05243012163434457
1112	20	-0.12228041486881835
1112	21	-0.2777708188803487
1112	22	-0.04172028433517738
1112	23	0.11093115363743067
1112	24	0.19549546772609006
1112	25	0.1756801528749321
1113	1	-0.5949651967535665
1113	2	-0.5459065462525956
1113	3	-0.5856986197840364
1113	4	-2.0013690824180657
1113	5	-0.6980408202513573
1113	6	-0.3697801598941197
1113	7	-0.49266572180129486
1113	8	-0.42268200326665806
1113	9	-0.3902884198654135
1113	10	-0.503037578157425
1113	11	-0.6681751165851111
1113	12	-0.22195393016476572
1113	13	-0.37939409456139117
1113	14	-0.5488270510621263
1113	15	-0.6021630518596908
1113	16	-0.8260872513895379
1113	17	-0.8939564465559329
1113	18	-0.8177020991077057
1113	19	-0.6405156729775499
1113	20	-0.4551333087466176
1113	21	-0.5095744525627381
1113	22	-0.5490131862092653
1113	23	-0.268911180755552
1113	24	-0.21378756277071886
1113	25	-0.4710452856898186
1114	1	-0.8220032548750318
1114	2	-2.1702846730017527
1114	3	-1.4504605262014536
1114	4	-0.9859417585850707
1114	5	-0.8259954786947503
1114	6	-0.4074054471465445
1114	7	-0.451789832231384
1114	8	-0.5624622486436763
1114	9	-0.779634961885094
1114	10	-0.9458400145447001
1114	11	-0.833215323233027
1114	12	-0.9493605057682188
1114	13	-0.9450979803348787
1114	14	-1.0351267183534247
1114	15	-1.1870002957661185
1114	16	-1.4610263590905106
1114	17	-1.4529209819631603
1114	18	-1.2692933297822648
1114	19	-1.6584452652790063
1114	20	-0.9882555557298147
1114	21	-1.2327378622533587
1114	22	-0.8940281181204455
1114	23	-0.6713096733104535
1114	24	-0.7285300003400366
1114	25	-1.4540430711083445
1115	1	-1.0358861098099545
1115	2	-1.1746172089955778
1115	3	-0.7535578741450202
1115	4	-0.5781299171564243
1115	5	-0.9038703555761529
1115	6	-0.7572777785975404
1115	7	-0.7228742001950437
1115	8	-0.9164227846260715
1115	9	-1.8707050082294643
1115	10	-1.7233024897740847
1115	11	-2.0033522854298624
1115	12	-2.614164260833906
1115	13	-2.481189794968845
1115	14	-2.067946143030248
1115	15	-2.391510732380169
1115	16	-2.122548195510241
1115	17	-2.062213613396338
1115	18	-2.4673514941693
1115	19	-2.975421148485232
1115	20	-3.18116044639288
1115	21	-2.3746902334199262
1115	22	-2.294686693448064
1115	23	-2.2431185074849083
1115	24	-2.86167087533412
1115	25	-2.809752042083005
1116	1	-1.1037813430647887
1116	2	-1.2623029308152773
1116	3	-1.214819859768273
1116	4	-0.8526595170199442
1116	5	-1.197351049004638
1116	6	-2.036466701826953
1116	7	-1.3869361908780178
1116	8	-1.684188330056056
1116	9	-2.7990915275717114
1116	10	-1.4590482620677534
1116	11	-2.389992769113391
1116	12	-4.517305683647351
1116	13	-3.39690584463586
1116	14	-2.702308697450647
1116	15	-3.864536978393609
1116	16	-3.3617395253512825
1116	17	-3.07384586692217
1116	18	-2.830172766280853
1116	19	-3.110484360961472
1116	20	-5.125438305598312
1116	21	-3.9017524514660735
1116	22	-3.138488030577051
1116	23	-3.813843536537771
1116	24	-3.641334471895192
1116	25	-2.954722904892197
1201	1	0.3120864593497572
1201	2	0.551390566063386
1201	3	0.5706490816251026
1201	4	0.47419015463456304
1201	5	-0.22546587726532152
1201	6	0.481528989630327
1201	7	0.587352143599233
1201	8	0.5621999718955166
1201	9	0.44734088711323494
1201	10	0.6008783316135293
1201	11	0.3222937448816907
1201	12	0.8411426983112378
1201	13	0.6804769340943295
1201	14	0.6950146067347234
1201	15	0.5454195470997973
1201	16	0.6688712133091812
1201	17	0.5064057753172477
1201	18	0.8188133405118947
1201	19	0.8311695098839564
1201	20	0.9586455130319678
1201	21	0.5945617035506814
1201	22	0.5618417995624725
1201	23	0.710442698958353
1201	24	0.37433510478980025
1201	25	0.5979409581272748
1202	1	0.6980882006898064
1202	2	0.6597763849507565
1202	3	0.6477471444008742
1202	4	0.5853918085760554
1202	5	0.5990213358876986
1202	6	0.7029806445664946
1202	7	0.804657837635304
1202	8	0.6057183872628116
1202	9	0.9553918494920737
1202	10	0.8550815690736897
1202	11	0.8927628125552332
1202	12	0.7681686307504307
1202	13	0.8525884382116047
1202	14	0.9248969402895355
1202	15	0.7859725618196265
1202	16	0.9286266299642492
1202	17	0.8172210487129448
1202	18	0.8316490093363385
1202	19	0.9184380993189407
1202	20	0.7161424428094136
1202	21	0.8319069822380314
1202	22	0.7429263274975213
1202	23	0.9800458710353794
1202	24	0.9532829269211334
1202	25	0.9804000903484251
1203	1	0.7003211578983048
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2211	8	-0.2401507405136769
2211	9	-0.049508119550573326
2211	10	-0.04996157774156984
2211	11	0.05420349052133133
2211	12	-0.04897497268583351
2211	13	0.07780320487053416
2211	14	-0.034853659509238355
2211	15	-0.13531545768351094
2211	16	-0.09962582727590785
2211	17	-0.15323402442380996
2211	18	-0.16440102549069024
2211	19	-0.17715434708613742
2211	20	-0.21164662150449232
2211	21	-0.10895866283588163
2211	22	0.003559211949188068
2211	23	-0.1953372262481139
2211	24	-0.05334194731460684
2211	25	-0.36903010081324794
2212	1	-0.020439196570468
2212	2	0.07235629362655871
2212	3	0.08758352187424023
2212	4	0.025825598530389016
2212	5	-0.22412934904381387
2212	6	0.011823871354920357
2212	7	-0.2373703206599771
2212	8	0.07664304479705919
2212	9	0.04106156029876473
2212	10	-0.19777231220484737
2212	11	0.006651701444120306
2212	12	0.26293651507310756
2212	13	0.20342938189502036
2212	14	0.19608796007644713
2212	15	0.07598397734474105
2212	16	0.09685722357155413
2212	17	-0.026972254178993182
2212	18	-0.1110488597656456
2212	19	0.1190697583752538
2212	20	0.10105111860850258
2212	21	0.3017852166368087
2212	22	0.20710440968930044
2212	23	-0.26651425073022494
2212	24	-0.18871859514888456
2212	25	-0.025235373882928513
2213	1	-0.5124208420233352
2213	2	-0.06271011478517607
2213	3	-0.19326118697406613
2213	4	-0.3589802490991971
2213	5	-0.14507474657194308
2213	6	-0.12845636599365662
2213	7	-0.6131881279526041
2213	8	-0.5666317200064839
2213	9	-0.31857396205258226
2213	10	-0.4001127475488815
2213	11	-0.2661331475997173
2213	12	-0.5918950043267586
2213	13	-0.42704454665445013
2213	14	-0.5043203286771671
2213	15	-0.6743019477613288
2213	16	-1.1517975449213438
2213	17	-0.9054101192715294
2213	18	-0.5339499235300167
2213	19	-0.44282441859086674
2213	20	-0.4272125781264151
2213	21	-0.3230696686856973
2213	22	-0.45102278086594794
2213	23	-0.5026770128282365
2213	24	-0.29248757996103336
2213	25	-0.41742100998942533
2214	1	-1.0324989449981388
2214	2	-0.459218452382153
2214	3	-0.5402252992836374
2214	4	-0.5835732887458533
2214	5	-0.8790954835382507
2214	6	-1.4543013503835027
2214	7	-0.9249328105809553
2214	8	-0.6554070277718793
2214	9	-0.5317762884353101
2214	10	-0.9509955826450991
2214	11	-0.8590151264868595
2214	12	-0.5482189437238603
2214	13	-0.9308525887548811
2214	14	-0.5729621931246527
2214	15	-0.6853125877254129
2214	16	-0.7134487754608614
2214	17	-0.8938074420519158
2214	18	-0.9159319431392916
2214	19	-0.8284056488171316
2214	20	-1.105636109257972
2214	21	-1.1620762573190007
2214	22	-1.642945179086702
2214	23	-1.5599354731137112
2214	24	-1.2255779726686384
2214	25	-1.73181576414256
2215	1	-1.297491366468897
2215	2	-0.8207046482016978
2215	3	-0.8431590142907339
2215	4	-0.9022884365573276
2215	5	-1.425177363817589
2215	6	-1.2191956731202964
2215	7	-1.604367858363883
2215	8	-1.1431422737624999
2215	9	-1.2423818068911388
2215	10	-1.3144043528327316
2215	11	-1.0343147497716387
2215	12	-1.2531222722460669
2215	13	-1.3709142623031951
2215	14	-1.611089633029124
2215	15	-1.4879126062194317
2215	16	-1.5676141522363878
2215	17	-1.6838528328185731
2215	18	-1.3638712666600696
2215	19	-1.7493682225419462
2215	20	-1.7060610697571477
2215	21	-1.3583702158039372
2215	22	-1.5302663197659143
2215	23	-1.969698307363032
2215	24	-1.8604283519971432
2215	25	-2.122694592103919
2216	1	-1.2940061496674957
2216	2	-0.7148563809169453
2216	3	-0.6816857823386719
2216	4	-0.9801079854295054
2216	5	-1.794460534149433
2216	6	-2.0614196541254444
2216	7	-1.8339540418677558
2216	8	-1.9218719927969268
2216	9	-3.8473001177057675
2216	10	-1.8588133075503563
2216	11	-2.0834724183792446
2216	12	-2.4739205084380522
2216	13	-2.3195885796755746
2216	14	-2.533315310003516
2216	15	-2.384775063113551
2216	16	-1.7591569189275518
2216	17	-2.089110337482488
2216	18	-2.4179613791593653
2216	19	-2.7617996771182405
2216	20	-2.0615616633463816
2216	21	-2.7167426506504526
2216	22	-2.1708395573712096
2216	23	-3.2538749142938954
2216	24	-2.490441354428029
2216	25	-2.5355641720688666
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62914.0
3	1.0
4	1041.0
5	9114.0
6	11197.0
7	8301.0
8	6893.0
9	5917.0
10	5037.0
11	4648.0
12	4170.0
13	3978.0
14	3860.0
15	3941.0
16	4013.0
17	4293.0
18	4614.0
19	5648.0
20	7290.0
21	9461.0
22	12012.0
23	14665.0
24	17128.0
25	19916.0
26	22183.0
27	24347.0
28	27756.0
29	33382.0
30	41959.0
31	53362.0
32	67365.0
33	86923.0
34	112232.0
35	147104.0
36	199942.0
37	310644.0
38	931781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.60024860971379	19.041641471213346	13.450458852381534	26.907651066691322
2	13.488979098048265	20.2065020524332	40.66343941650757	25.64107943301096
3	17.59131735742013	25.169840934155722	29.375446777348017	27.86339493107613
4	11.676922572823486	16.87661238354249	36.75795406140437	34.688510982229644
5	12.842970403885268	38.84097358917613	34.79530464532507	13.52075136161353
6	32.21884857216794	36.404428272683404	17.960286125934577	13.416437029214082
7	28.69921592527725	31.411867885114297	21.42700375380036	18.461912435808088
8	25.889091209485738	36.856931559011805	19.96435155763063	17.289625673871825
9	27.378671914735197	14.37218057597086	19.557386475853235	38.691761033440706
10	15.922167151810768	28.788276121294764	33.21702986914221	22.072526857752255
11	34.788160936262386	21.983493604656495	24.36373136630156	18.864614092779554
12	24.35206179036535	24.46341900280905	30.79286859499439	20.391650611831214
13	29.5454495808287	21.738146489109933	25.54312708913938	23.17327684092199
14	21.34846824743601	22.143003182158942	27.122618841666185	29.385909728738863
15	23.541825506229426	31.163284521799163	22.761743932783833	22.533146039187585
16	22.30565129801729	27.496890342125717	27.724385790687585	22.473072569169407
17	21.77367281013782	28.54490343539831	27.395241627762097	22.286182126701775
18	21.68963676287224	26.719742690651216	30.115408240924314	21.47521230555223
19	23.98350541766383	26.152580565960413	27.417240936411368	22.446673079964384
20	24.631827090011996	26.295169169499776	27.79319931883097	21.27980442165726
21	23.584441592188117	26.011456401106848	26.847969398337984	23.556132608367054
22	24.006925213201942	27.641002050216095	26.660760226943587	21.691312509638376
23	22.499510710204138	27.34430973442049	27.82958036410151	22.326599191273864
24	23.7583397698241	26.210948558167818	28.177675104585692	21.853036567422386
25	22.90322848401791	26.87683128799916	27.960696032513322	22.25924419546961
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	110.0
1	110.0
2	297.0
3	484.0
4	484.0
5	484.0
6	1318.0
7	2152.0
8	2152.0
9	2152.0
10	2548.5
11	2945.0
12	2945.0
13	2945.0
14	4623.0
15	6301.0
16	6301.0
17	6301.0
18	13077.5
19	19854.0
20	19854.0
21	19854.0
22	34335.5
23	48817.0
24	48817.0
25	48817.0
26	75562.5
27	102308.0
28	102308.0
29	102308.0
30	133485.0
31	164662.0
32	164662.0
33	164662.0
34	187007.0
35	209352.0
36	209352.0
37	209352.0
38	225803.0
39	242254.0
40	242254.0
41	242254.0
42	259188.5
43	276123.0
44	276123.0
45	276123.0
46	294174.5
47	312226.0
48	312226.0
49	312226.0
50	301532.5
51	290839.0
52	290839.0
53	290839.0
54	258753.0
55	226667.0
56	226667.0
57	226667.0
58	195664.5
59	164662.0
60	164662.0
61	164662.0
62	137375.5
63	110089.0
64	110089.0
65	110089.0
66	85087.5
67	60086.0
68	60086.0
69	60086.0
70	44909.0
71	29732.0
72	29732.0
73	29732.0
74	21102.5
75	12473.0
76	12473.0
77	12473.0
78	8631.0
79	4789.0
80	4789.0
81	4789.0
82	3170.5
83	1552.0
84	1552.0
85	1552.0
86	1003.5
87	455.0
88	455.0
89	455.0
90	271.0
91	87.0
92	87.0
93	87.0
94	48.5
95	10.0
96	10.0
97	10.0
98	6.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08357244459666793
2	0.04438557433884716
3	0.018348367344799024
4	0.004674465014032132
5	0.016906709910564817
6	0.059763253637345394
7	0.07645153060332927
8	0.04954059183095737
9	0.01939684547878754
10	0.045958291539829936
11	0.004412345480535004
12	8.737317783237631E-5
13	0.004849211369696885
14	8.737317783237631E-4
15	0.01297491690810788
16	0.00851888483865669
17	0.004281285713786439
18	0.004980271136445449
19	0.0016600903788151497
20	0.001135851311820892
21	2.621195334971289E-4
22	2.1843294458094077E-4
23	0.0
24	0.0
25	4.3686588916188156E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2289032.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.7149839151055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.32382500785172	39.38588080890765
2	14.726336496567537	15.820498560831126
3	5.282943307993832	8.513196444399085
4	2.4544286533491113	5.273583825416862
5	1.3061258027316662	3.507926324241785
6	0.7819499375892686	2.5201456992015183
7	0.4735483230052429	1.780564838727626
8	0.34091454671991933	1.4649775514788757
9	0.2381589585387374	1.15134341644319
>10	0.9687873589934701	8.793145084873846
>50	0.05511358880248366	2.056571298327517
>100	0.04003155675119923	4.207389255198253
>500	0.0048977881910625375	1.8992516751978925
>1k	0.002693783505084213	2.829431175085737
>5k	2.4488940955311026E-4	0.7960940416689649
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7221	0.3154608585637947	No Hit
GTCCTACAGTGGACATTTCTAAATT	5722	0.24997466177842861	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5213	0.22773818802008886	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4937	0.2156806894792209	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4891	0.21367110638907624	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4787	0.2091277011417927	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4201	0.18352736003690642	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3121	0.13634584400742322	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2469	0.10786218803406854	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2459	0.10742532214490666	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2292	0.10012966179590325	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.3686588916188156E-5	0.0
11	0.0	0.0	0.0	8.737317783237631E-5	0.0
12	0.0	0.0	0.0	8.737317783237631E-5	0.0
13	0.0	0.0	0.0	8.737317783237631E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	885	0.0	13.215447	4
ATACCGT	55	0.003049296	12.10142	6
TCCAACG	160	3.6379788E-12	11.866631	18
GTCCTAG	270	0.0	11.272007	1
CTAACCG	60	0.005908016	11.075522	18
GATATAC	405	0.0	10.8023405	1
AGGACCT	1830	0.0	10.703755	5
TGGACCG	80	3.740456E-4	10.69726	5
GGTATCA	2045	0.0	10.696694	1
GGCGAGG	1200	0.0	10.363297	19
TGTAGGA	2870	0.0	10.338314	2
GTAGGAC	2865	0.0	10.322938	3
GGACCTG	1845	0.0	10.255521	6
GTCCTAA	1525	0.0	10.165595	1
CTGTAGG	2905	0.0	10.149172	1
GCGTGCG	150	2.4563633E-8	10.130618	9
GTCCTAC	2765	0.0	10.112703	1
GTCTAGA	170	1.5297701E-9	10.070212	1
GCACCGT	85	6.542995E-4	10.067569	6
CTGTTCG	85	6.6117523E-4	10.056128	9
>>END_MODULE