FastQCFastQC Report
Thu 26 May 2016
SRR1512888_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512888_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2688438
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT127160.4729884044192204No Hit
GTCCTACAGTGGACATTTCTAAATT101230.3765383468021208No Hit
CTGTAGGACGTGGAATATGGCAAGA84550.3144948851340444No Hit
GTCCTAAAGTGTGTATTTCTCATTT78620.29243746740672466No Hit
TATCAACGCAGAGTACTTTTTTTTT74490.2770753872694851No Hit
GGTATCAACGCAGAGTACTTTTTTT70870.2636103194494349No Hit
CTTTAGGACGTGAAATATGGCGAGG69440.2582912456973157No Hit
GTCCTACAGTGTGCATTTCTCATTT55440.2062163977744698No Hit
CTGTAGGACCTGGAATATGGCGAGA38620.14365218762716492No Hit
CTGAAGGACCTGGAATATGGCGAGA36560.1359897457185176No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35940.13368357388193441No Hit
GTCCTTCAGTGTGCATTTCTCATTT30540.11359756111169385No Hit
TTTCTAAATTTTCCACCTTTTTCAG26970.10031847489136815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAGT250.006037705618.9975418
TAGGACC13800.013.8351664
TCCAACG2350.012.93449718
GGTATCA26800.012.6846231
TGTAGGA47250.011.3985262
GGCGAGG18450.011.3777219
GCCGGTT1604.5474735E-1111.2795811
CTACACG1104.981066E-711.22582154
CGATTTG600.005882391711.08210614
GTAGGAC46300.011.0784813
CTGTAGG48400.011.0766171
AGGACGT45200.011.0748955
GTCCTAC44900.011.0705261
GGACGTG44750.011.0376786
CCAACGA1855.456968E-1210.78218819
TAATACT3300.010.6501384
GTCGAGG1355.7050784E-810.55399319
GACGTGA23100.010.5267767
AGGACCT26650.010.5145875
TAGGACG49050.010.4960944
Produced by FastQC (version 0.11.5)