##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512888_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2688438
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.948362952762906	31.0	30.0	34.0	23.0	34.0
2	29.09413272688453	31.0	30.0	34.0	22.0	34.0
3	29.200543587019673	31.0	30.0	34.0	23.0	34.0
4	32.63052300257622	35.0	33.0	37.0	25.0	37.0
5	32.81489511753665	35.0	33.0	37.0	26.0	37.0
6	32.79430174696236	36.0	33.0	37.0	25.0	37.0
7	32.780134412621756	37.0	35.0	37.0	25.0	37.0
8	32.7042390414062	37.0	33.0	37.0	25.0	37.0
9	34.00549389645586	39.0	34.0	39.0	23.0	39.0
10	33.85943733870746	39.0	34.0	39.0	23.0	39.0
11	33.921086891347315	39.0	34.0	39.0	23.0	39.0
12	33.81395181886285	39.0	34.0	39.0	22.0	39.0
13	33.788331365648006	39.0	34.0	39.0	22.0	39.0
14	34.65843400517326	39.0	34.0	41.0	18.0	41.0
15	34.84537861762109	39.0	34.0	41.0	22.0	41.0
16	34.796121018970865	39.0	34.0	41.0	22.0	41.0
17	34.5789692750958	39.0	34.0	41.0	18.0	41.0
18	34.5845736446219	39.0	34.0	41.0	18.0	41.0
19	34.70540588996287	39.0	34.0	41.0	18.0	41.0
20	34.68472362018392	40.0	34.0	41.0	18.0	41.0
21	34.585203750281764	39.0	34.0	41.0	18.0	41.0
22	34.60837482582823	39.0	34.0	41.0	18.0	41.0
23	34.294155193461776	39.0	34.0	41.0	16.0	41.0
24	34.369126236126704	39.0	34.0	41.0	16.0	41.0
25	34.101453334612884	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6386015754148033
1101	2	0.30838071012490076
1101	3	0.702978502690403
1101	4	0.819327194145778
1101	5	0.7526860369959962
1101	6	0.7857268724325053
1101	7	0.6601506223747933
1101	8	0.8596226226125481
1101	9	0.8783493804430833
1101	10	0.9657816985644558
1101	11	0.9447758071271224
1101	12	0.7586556507911766
1101	13	1.1990496259476444
1101	14	1.2412486208562896
1101	15	1.1778629554486955
1101	16	0.9898772544695902
1101	17	0.3552220615144037
1101	18	0.7815238149036787
1101	19	0.310028198290496
1101	20	-0.1176094539571011
1101	21	0.844805713343689
1101	22	0.8610528558370518
1101	23	0.5159761465709067
1101	24	1.0344653849012033
1101	25	0.7034682784945545
1102	1	0.8006257831880532
1102	2	0.8653930573057274
1102	3	0.7979629926849299
1102	4	0.9844254241804506
1102	5	0.9975324575300277
1102	6	0.7771890705956324
1102	7	0.8759994150273087
1102	8	0.8863211202708428
1102	9	1.1326562960808246
1102	10	1.1486404054557653
1102	11	0.9801483513762577
1102	12	1.1046698835020834
1102	13	1.012463651858475
1102	14	1.2525611930842544
1102	15	1.1297132078664305
1102	16	1.2152753446100917
1102	17	1.282279418122883
1102	18	1.2224842187720597
1102	19	1.123182507639072
1102	20	1.3560557732188627
1102	21	1.4378943332573257
1102	22	1.2995523388368682
1102	23	1.2860278465891497
1102	24	0.6077784437175779
1102	25	0.9767594408326161
1103	1	0.6274380051637891
1103	2	0.7569369165451825
1103	3	0.8380865201049801
1103	4	0.916028595262425
1103	5	0.7424637468620716
1103	6	0.3947879917739314
1103	7	0.33536111218664644
1103	8	0.6332621236684304
1103	9	0.7104769361349952
1103	10	0.8868472730198889
1103	11	0.7557342893323309
1103	12	0.788102498010133
1103	13	0.997452851965889
1103	14	1.0410361314956802
1103	15	0.9450627157415923
1103	16	1.0041376780874245
1103	17	0.6960419839438501
1103	18	1.010824187715066
1103	19	0.9265610643064051
1103	20	1.0728128865535211
1103	21	0.9200023555342938
1103	22	0.964942127392483
1103	23	0.32183118346407014
1103	24	0.5102646373684081
1103	25	0.7849717959097333
1104	1	1.022590376859963
1104	2	0.9124400663039545
1104	3	1.0029212864237103
1104	4	1.0994394435847141
1104	5	0.8936694370816411
1104	6	0.861188530936559
1104	7	1.053483547877292
1104	8	0.8872391272864135
1104	9	1.1830189235166202
1104	10	1.1340107171675342
1104	11	0.9079954636136094
1104	12	1.234417064956581
1104	13	1.1495750679942844
1104	14	1.4246126014771292
1104	15	1.1419502120546525
1104	16	1.2359345499034475
1104	17	1.536897022740483
1104	18	1.3594265808363701
1104	19	0.945403204737822
1104	20	1.0749031318223174
1104	21	1.1813248052243779
1104	22	0.9651827427599571
1104	23	1.2128479641409768
1104	24	1.1928972273729457
1104	25	0.88827154210081
1105	1	0.6125556908366434
1105	2	0.7561531804409398
1105	3	0.8378824812002037
1105	4	1.0126660114406931
1105	5	0.8044801250649556
1105	6	0.8689982110171357
1105	7	0.8761154462847074
1105	8	0.8552413191815731
1105	9	0.527404105694842
1105	10	0.6808118475585303
1105	11	0.7016307098891588
1105	12	0.8966755666657136
1105	13	0.9179052810320485
1105	14	1.05351549097675
1105	15	1.0600920854416103
1105	16	0.9483537249084577
1105	17	1.252312569910707
1105	18	1.0603506644268066
1105	19	0.8818588041107773
1105	20	1.011705785058787
1105	21	0.9506554035864738
1105	22	0.9723418249841558
1105	23	1.1563901482704182
1105	24	1.2552044441911718
1105	25	1.4178345059522002
1106	1	0.49987755516680465
1106	2	0.5274921933735612
1106	3	0.4411770098214589
1106	4	0.5252385507385142
1106	5	0.3561088346994836
1106	6	0.5634492331972609
1106	7	0.5402073390439099
1106	8	0.5448443824881721
1106	9	0.7657809593012317
1106	10	0.47494669575828397
1106	11	0.30092748910207945
1106	12	0.5083596258619991
1106	13	0.48344370266408276
1106	14	0.7997244875139913
1106	15	0.5265610693530576
1106	16	0.5604968937665902
1106	17	0.8034860216674318
1106	18	0.6485740819285155
1106	19	0.7037932999815197
1106	20	0.8138517691686289
1106	21	0.7154034966228124
1106	22	0.6555105692858874
1106	23	1.0161625584242557
1106	24	0.5804976095108358
1106	25	0.8009186317326069
1107	1	1.045949982610523
1107	2	1.018762153239937
1107	3	1.0197232781853138
1107	4	1.0996605173114418
1107	5	0.9365491924008182
1107	6	1.0704113072029529
1107	7	0.7763635518558445
1107	8	0.9388744123627291
1107	9	0.9940727652651304
1107	10	1.1797232467249756
1107	11	1.0607100080890604
1107	12	1.1044505795781419
1107	13	0.9542235996218125
1107	14	1.1862450036588044
1107	15	1.0961471549430328
1107	16	1.1161759204517594
1107	17	1.464128021861633
1107	18	1.3999223143854138
1107	19	1.1504986238881685
1107	20	1.3414819129406794
1107	21	1.1546584182995119
1107	22	1.1522826051425312
1107	23	1.5584293258774267
1107	24	1.3351564221361798
1107	25	1.6340872652994136
1108	1	0.8706882370797366
1108	2	0.8398616360714044
1108	3	0.8166296668603295
1108	4	0.9406740233323347
1108	5	0.8267499536301841
1108	6	0.9433461007413229
1108	7	0.897138733947628
1108	8	0.9661694626240447
1108	9	0.9665849218136273
1108	10	1.0289627553890313
1108	11	0.8097102730864449
1108	12	0.8497311829259715
1108	13	1.0058204499842986
1108	14	1.0081525340126731
1108	15	1.046243666105667
1108	16	0.769790608926904
1108	17	1.0820138905311012
1108	18	1.1618542172515376
1108	19	1.1379570485269426
1108	20	1.181243275682668
1108	21	1.1418270137062692
1108	22	1.1260703513501724
1108	23	1.4708416956973949
1108	24	1.3576427796733341
1108	25	1.414380561398474
1109	1	0.5678007220703094
1109	2	0.6259672798311691
1109	3	0.718705502207925
1109	4	0.7147500588953264
1109	5	0.5785956220078248
1109	6	0.6133326830807064
1109	7	0.6258186202123639
1109	8	0.4785293185766051
1109	9	0.6982518387495347
1109	10	0.6487700319268512
1109	11	0.5537830094011582
1109	12	0.5568797874193052
1109	13	0.5963024147216274
1109	14	0.7016651785297014
1109	15	0.5866277567321987
1109	16	0.2531950671639649
1109	17	0.665845851689312
1109	18	0.7345218559316464
1109	19	0.713242772577388
1109	20	0.6645912215104133
1109	21	0.6028366267361989
1109	22	0.655011432588644
1109	23	0.7837548350478443
1109	24	0.7544689889447085
1109	25	0.3387913926206636
1110	1	0.5721457426783054
1110	2	0.6390200863095821
1110	3	0.5358936197420654
1110	4	0.49379442859713407
1110	5	0.48546280326947056
1110	6	0.5286164765651975
1110	7	0.44439646681789213
1110	8	0.4447072006714379
1110	9	0.585087724893377
1110	10	0.39839537774597034
1110	11	0.5188599163465781
1110	12	0.5042636607547308
1110	13	0.5365843934119141
1110	14	0.5136541123179441
1110	15	0.46780390469034927
1110	16	0.5505922489902062
1110	17	0.5582309752163042
1110	18	-0.06072983585983849
1110	19	0.3378844515443262
1110	20	0.5444792688725073
1110	21	0.6710535309392682
1110	22	0.6608236134873309
1110	23	0.8510111081864764
1110	24	0.7219180335563777
1110	25	0.9668553701656109
1111	1	0.29192271752113896
1111	2	0.37395728532641215
1111	3	0.4542655747472857
1111	4	0.3416272155607061
1111	5	0.36411981002212457
1111	6	0.19869863200262472
1111	7	0.12157920126501409
1111	8	0.2842008094470714
1111	9	0.3901869612641349
1111	10	0.4173340487803756
1111	11	0.0906291746392256
1111	12	-0.2640424909849415
1111	13	0.15957320645414086
1111	14	0.19165977008352542
1111	15	0.13725113847031167
1111	16	0.27756616577290316
1111	17	-0.09390163842819987
1111	18	0.09964009042386124
1111	19	0.2194499545816342
1111	20	0.33173472109514535
1111	21	0.238830948138947
1111	22	-0.031672452140384166
1111	23	0.19441804508271332
1111	24	0.19901294084262844
1111	25	0.26385332936516903
1112	1	0.4418652653160855
1112	2	0.35032518197196083
1112	3	0.35642710290100865
1112	4	0.4785194848082668
1112	5	0.40528281093052954
1112	6	0.24989510869556852
1112	7	0.1615797771786731
1112	8	0.3181036050690125
1112	9	0.25174844946785413
1112	10	0.3850078970975588
1112	11	0.2754291645339748
1112	12	0.35914763609929423
1112	13	0.3601585830856209
1112	14	0.19507403891188346
1112	15	0.04278700686568726
1112	16	0.2903849548598316
1112	17	0.5902830979756857
1112	18	0.29379236156829336
1112	19	0.4422147512273753
1112	20	0.1797872337165458
1112	21	0.1761156629407239
1112	22	0.21275627897194482
1112	23	0.3957258294015489
1112	24	0.472365918755159
1112	25	0.4206840936026026
1113	1	-0.19378992936041683
1113	2	-0.08863075932002218
1113	3	-0.02043110593150388
1113	4	-0.04923540687753558
1113	5	-0.11163763172495322
1113	6	-0.44368466457633815
1113	7	-0.3090947730735607
1113	8	-0.2689802587072023
1113	9	-0.2006635763464999
1113	10	-0.15464160817340655
1113	11	-0.27612771956884075
1113	12	-0.4363968168168526
1113	13	-0.3250869986245064
1113	14	-0.10982712346118717
1113	15	-0.2524761779032545
1113	16	-0.33535012862488145
1113	17	-0.2469303655083479
1113	18	-0.3432464560323396
1113	19	-0.8480576548095868
1113	20	-0.525813074726436
1113	21	-0.28071292186508856
1113	22	-0.6998513277303715
1113	23	-4.56759420788163
1113	24	-2.160555998610853
1113	25	-1.296830949916071
1114	1	-0.44496561594731787
1114	2	-0.6021997888605384
1114	3	-0.4039101349946215
1114	4	-0.40400562360147063
1114	5	-0.3668692669481146
1114	6	-1.0670157500502704
1114	7	-0.9047301396818206
1114	8	-0.7587751192426673
1114	9	-0.9859054030443843
1114	10	-0.5378174183771947
1114	11	-0.7096114534539595
1114	12	-1.6994456637087936
1114	13	-0.8728965811015357
1114	14	-2.5910160227565484
1114	15	-1.4580978290328588
1114	16	-1.418694781975212
1114	17	-1.3958647348557918
1114	18	-1.330467963434245
1114	19	-1.4715485658545475
1114	20	-0.9853578324592362
1114	21	-0.8124284383449947
1114	22	-1.0599530994879203
1114	23	-5.113901476116677
1114	24	-2.408327608100482
1114	25	-2.3656225394471058
1115	1	-1.6031603807666777
1115	2	-1.5329107485814149
1115	3	-1.1018418394959753
1115	4	-1.242966413630736
1115	5	-1.2125692544884998
1115	6	-1.2948018246306674
1115	7	-1.9155607808285318
1115	8	-2.290089603638936
1115	9	-3.40413341116745
1115	10	-3.976312407965178
1115	11	-2.9040653011975053
1115	12	-3.0778468869406588
1115	13	-2.485619988216289
1115	14	-3.0421471445491903
1115	15	-2.7022107202586056
1115	16	-2.8538978868965508
1115	17	-2.71816722033504
1115	18	-5.120265904497863
1115	19	-3.311547387896834
1115	20	-3.652346189926295
1115	21	-3.221135217122807
1115	22	-2.8109868380640677
1115	23	-3.302997575681996
1115	24	-3.041937519450517
1115	25	-3.339047317799146
1116	1	-1.5300097084906774
1116	2	-1.4247904250757593
1116	3	-1.8370574576426968
1116	4	-1.5936634761865172
1116	5	-1.8188087055861075
1116	6	-4.015143888365575
1116	7	-2.654684304589992
1116	8	-3.004927280639663
1116	9	-4.086148756141085
1116	10	-3.2798535975832586
1116	11	-2.8264753926252837
1116	12	-2.6147811708459407
1116	13	-3.8531217800608495
1116	14	-6.650818306137971
1116	15	-4.904580099681272
1116	16	-3.9016745235588672
1116	17	-4.939303935730848
1116	18	-3.515595612580306
1116	19	-3.273827540348627
1116	20	-4.027915073803076
1116	21	-4.715300362270032
1116	22	-4.404338148307353
1116	23	-3.605204158023433
1116	24	-4.427999359418742
1116	25	-4.359205988804444
1201	1	0.8210017216796004
1201	2	0.8927790083807388
1201	3	1.234885282273165
1201	4	1.2103884203692843
1201	5	0.8624413327851599
1201	6	1.273561962564493
1201	7	1.3838434884569324
1201	8	1.443020459789544
1201	9	1.7709108673700555
1201	10	1.5652922408953742
1201	11	1.6933293184364828
1201	12	1.6120416254989394
1201	13	1.767035302339238
1201	14	1.8641253958405741
1201	15	1.81557114116805
1201	16	1.7331928115408743
1201	17	1.8820449081253514
1201	18	1.6936070971941248
1201	19	1.8600082838701724
1201	20	1.9050245313093086
1201	21	2.011294637909593
1201	22	1.9054143662796577
1201	23	2.2611630435594847
1201	24	2.0593643815468354
1201	25	2.034108845676947
1202	1	0.7387750810446683
1202	2	0.7673472332111224
1202	3	0.806090117592543
1202	4	0.9723071684524385
1202	5	0.8732249926371978
1202	6	1.050479536227563
1202	7	1.1620703684641143
1202	8	1.1602915563388407
1202	9	1.356452304950004
1202	10	1.1392035983604174
1202	11	1.1542119138300606
1202	12	1.338455544995064
1202	13	1.247183533602751
1202	14	1.6722759011404236
1202	15	1.3398492019536405
1202	16	1.2448407548096512
1202	17	1.5757945696380347
1202	18	1.5294775668873584
1202	19	1.5538861292207429
1202	20	1.35134180426099
1202	21	1.5470044589040342
1202	22	1.4631606167378308
1202	23	1.668593995887008
1202	24	1.647009781485501
1202	25	1.9075000173292906
1203	1	1.0910366285586406
1203	2	1.2574437321814074
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2211	15	-0.10183628998149175
2211	16	-0.17787147509339718
2211	17	0.29885043677424505
2211	18	-0.07437374392521434
2211	19	0.05461722786862566
2211	20	-0.10627325595378068
2211	21	-0.05750021480580614
2211	22	-0.061648043755191395
2211	23	0.07616653621133906
2211	24	-0.19907951897970122
2211	25	0.22961453408942845
2212	1	0.012019758994224361
2212	2	0.11376292016115386
2212	3	-0.20024661783372366
2212	4	-0.011581323286364409
2212	5	-0.15937641700637073
2212	6	-0.33430854352783257
2212	7	-0.21279066249923773
2212	8	-0.10579046711693962
2212	9	0.04244636205348229
2212	10	-0.3328003635705912
2212	11	-0.40694319002707857
2212	12	-0.07734210942694375
2212	13	-0.05492829046454517
2212	14	-0.011214962015053231
2212	15	-0.33121583974711655
2212	16	-0.4008327303977026
2212	17	-0.21504412522150318
2212	18	-0.018847828229134223
2212	19	-0.2163002250849857
2212	20	-0.05634882060442692
2212	21	0.1528130517007824
2212	22	0.10913894095585164
2212	23	0.15649127277718122
2212	24	0.1295801560028167
2212	25	0.17375797348965705
2213	1	-0.45873440474810323
2213	2	-0.3488440278413272
2213	3	-0.5557072899102486
2213	4	-0.3960121722635037
2213	5	-0.42067800332812055
2213	6	-0.34778778285561884
2213	7	-0.5217361385321979
2213	8	-0.2877731702471351
2213	9	-0.7383204652448683
2213	10	-0.46741293524761574
2213	11	-0.5990805576267135
2213	12	-0.5910642421111305
2213	13	-0.5828162947086923
2213	14	-0.6974770390261114
2213	15	-0.6116910160908233
2213	16	-0.5055065711410762
2213	17	-0.42182823060232977
2213	18	-0.5331600844131827
2213	19	-0.5096528870038242
2213	20	-0.2972542984087667
2213	21	-0.5386327631751158
2213	22	-0.7536370482977972
2213	23	-0.7580457156762179
2213	24	-0.556170749032141
2213	25	-0.34128150898198584
2214	1	-2.720139586208983
2214	2	-1.4559451580134706
2214	3	-1.6466788309730163
2214	4	-3.6288882237260047
2214	5	-1.8416253999081107
2214	6	-1.6347751412983023
2214	7	-1.4993392727421373
2214	8	-1.2671041440060122
2214	9	-1.1090894075747642
2214	10	-1.0857209008691626
2214	11	-1.2075962892662773
2214	12	-1.197064008897371
2214	13	-1.4983684511356756
2214	14	-1.4402262633617369
2214	15	-1.7225935304551925
2214	16	-1.5566498901386225
2214	17	-3.516873124250001
2214	18	-3.3558651581841836
2214	19	-2.238419689254563
2214	20	-2.1348725915127247
2214	21	-2.0427121747448
2214	22	-3.0763651004828034
2214	23	-2.0477972287076085
2214	24	-2.998276006173171
2214	25	-3.061263354891711
2215	1	-1.8430046917552403
2215	2	-3.446089298936819
2215	3	-2.0559415509626184
2215	4	-3.5280781419865654
2215	5	-2.1385971404869473
2215	6	-2.141168855649017
2215	7	-2.412101513345352
2215	8	-1.7184131125940176
2215	9	-4.024866094771085
2215	10	-2.433523848689177
2215	11	-2.0333523384724472
2215	12	-1.9200631801276913
2215	13	-2.003588075101746
2215	14	-1.7904180179159113
2215	15	-2.0039551502936632
2215	16	-2.0522517231821595
2215	17	-2.3781232664408734
2215	18	-2.031494454379967
2215	19	-2.0145454347410237
2215	20	-1.9570511182527355
2215	21	-2.6380336886307596
2215	22	-2.480096189096159
2215	23	-2.492513415717049
2215	24	-2.2356088303277417
2215	25	-3.166788486113578
2216	1	-3.258384930283569
2216	2	-3.449971656621784
2216	3	-4.588304388213178
2216	4	-5.14289050925926
2216	5	-4.908619810534706
2216	6	-3.866379427238879
2216	7	-5.512526912759334
2216	8	-3.580940106079737
2216	9	-4.22983401620548
2216	10	-3.330208466210525
2216	11	-3.5236629091662586
2216	12	-3.877798773894771
2216	13	-3.5523539911777675
2216	14	-4.450306370170818
2216	15	-3.8063057116685606
2216	16	-4.148581505173357
2216	17	-3.863281329540669
2216	18	-3.534174089867104
2216	19	-4.17880404353485
2216	20	-4.797263123521503
2216	21	-4.490243346250313
2216	22	-4.0554089103083335
2216	23	-4.07686593047946
2216	24	-4.653676992799404
2216	25	-4.583802446561961
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	124515.0
3	3.0
4	1104.0
5	6897.0
6	8473.0
7	7918.0
8	7046.0
9	6590.0
10	5826.0
11	5377.0
12	5188.0
13	4919.0
14	4856.0
15	4995.0
16	5217.0
17	5533.0
18	6356.0
19	7750.0
20	9872.0
21	13009.0
22	16274.0
23	19773.0
24	23070.0
25	25972.0
26	28956.0
27	31832.0
28	36037.0
29	42022.0
30	50917.0
31	62697.0
32	78675.0
33	100154.0
34	129698.0
35	170551.0
36	235365.0
37	370798.0
38	1024203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.94560006568598	19.137610261941507	12.820634354887167	24.096155317485348
2	14.073755258188788	20.384288598862877	43.44149293129796	22.100463211650375
3	19.301397762464077	26.249700080908404	28.928642505742637	25.52025965088488
4	11.63691717299024	16.802105819983787	39.17454594558601	32.386431061439964
5	12.23367173462593	39.68859392960906	35.419529561442346	12.658204774322671
6	30.532512880073853	37.479265314631164	18.98921200750469	12.999009797790286
7	27.897896310502663	32.05866893394999	22.20659576939227	17.83683898615508
8	24.748389056981175	38.06047896744752	20.742571566232044	16.44856040933926
9	27.50714849701943	14.984091080842326	20.53183105386771	36.97692936827054
10	15.618007813750681	29.378258056301597	34.188807736610926	20.814926393336794
11	33.57380105533785	22.684961472797166	25.63557413312863	18.10566333873635
12	24.360729981577705	25.047321618719554	31.96059684065832	18.631351559044422
13	29.270003294561743	22.924677384229657	26.050862821522237	21.754456499686363
14	20.380749504024944	23.651709173407173	28.088103188126528	27.879438134441347
15	22.244972109465508	33.2806818215661	23.496360679855975	20.977985389112416
16	20.60415679351579	28.745899811515113	29.881350941140074	20.768592453829022
17	20.45179447789374	29.824861876601698	28.825019370226556	20.898324275278007
18	20.178928736053305	27.498862520208416	32.38514805109313	19.937060692645144
19	22.969253898271514	26.87966271695433	28.760796058279915	21.39028732649425
20	23.486079909932116	27.484033724823842	29.618119907340873	19.41176645790317
21	22.232078808682417	26.990737828160118	28.08860355663527	22.688579806522196
22	22.660133921523656	29.355167565732053	28.188864794664585	19.795833718079702
23	20.94532232837579	28.919808737422002	29.504594479178802	20.630274455023397
24	22.570438265997527	26.97685067981117	30.359451087846796	20.093259966344508
25	21.60908653976813	28.118900272255175	29.867133666383356	20.40487952159334
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	450.0
1	450.0
2	1019.5
3	1589.0
4	1589.0
5	1589.0
6	3090.0
7	4591.0
8	4591.0
9	4591.0
10	5860.0
11	7129.0
12	7129.0
13	7129.0
14	10005.0
15	12881.0
16	12881.0
17	12881.0
18	23429.0
19	33977.0
20	33977.0
21	33977.0
22	54815.0
23	75653.0
24	75653.0
25	75653.0
26	112153.0
27	148653.0
28	148653.0
29	148653.0
30	193178.0
31	237703.0
32	237703.0
33	237703.0
34	264101.5
35	290500.0
36	290500.0
37	290500.0
38	304821.0
39	319142.0
40	319142.0
41	319142.0
42	329697.5
43	340253.0
44	340253.0
45	340253.0
46	350709.0
47	361165.0
48	361165.0
49	361165.0
50	337198.5
51	313232.0
52	313232.0
53	313232.0
54	266266.5
55	219301.0
56	219301.0
57	219301.0
58	183577.5
59	147854.0
60	147854.0
61	147854.0
62	119728.5
63	91603.0
64	91603.0
65	91603.0
66	69691.0
67	47779.0
68	47779.0
69	47779.0
70	34689.5
71	21600.0
72	21600.0
73	21600.0
74	15100.5
75	8601.0
76	8601.0
77	8601.0
78	5916.0
79	3231.0
80	3231.0
81	3231.0
82	2147.5
83	1064.0
84	1064.0
85	1064.0
86	717.5
87	371.0
88	371.0
89	371.0
90	231.5
91	92.0
92	92.0
93	92.0
94	55.0
95	18.0
96	18.0
97	18.0
98	12.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33562239486274187
2	0.009299079986222484
3	0.007922816148261555
4	0.010005810065175392
5	0.0114564665430261
6	0.07878180564327687
7	0.07848423508371775
8	0.10206670192877798
9	0.08216667075826185
10	0.0894199531475154
11	0.0849563947541286
12	0.095296971698808
13	0.08157152963914362
14	0.08610948067242019
15	0.08265022291754544
16	0.08484480579429393
17	0.09726837665588717
18	0.09953735217252545
19	0.09313958514200439
20	0.0916517323442088
21	0.07941414308234
22	0.09990931537197437
23	0.09287921090239015
24	0.08919677522784605
25	0.08759733347021578
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2688438.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.22812310039711
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7168203216815	44.47587760340632
2	13.154018393680852	15.055595677969114
3	4.262869743175144	7.31868103270156
4	1.8226283522006708	4.172223988240558
5	0.8999221895003838	2.5750428920753428
6	0.5336777063635217	1.8324824085425522
7	0.3217416735904965	1.2888870471935303
8	0.23388122472694917	1.0707666815636392
9	0.16296793314735442	0.8393714045616676
>10	0.794443422406292	8.088582811870836
>50	0.053084327275335304	2.083688444882508
>100	0.03643800808131403	4.155578020469536
>500	0.0038512656178019003	1.4792622767536763
>1k	0.00313323304496103	3.091736232539273
>5k	3.9165413062012877E-4	1.6190486208313353
>10k+	1.3055137687337627E-4	0.853174856398488
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	12716	0.4729884044192204	No Hit
GTCCTACAGTGGACATTTCTAAATT	10123	0.3765383468021208	No Hit
CTGTAGGACGTGGAATATGGCAAGA	8455	0.3144948851340444	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	7862	0.29243746740672466	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7449	0.2770753872694851	No Hit
GGTATCAACGCAGAGTACTTTTTTT	7087	0.2636103194494349	No Hit
CTTTAGGACGTGAAATATGGCGAGG	6944	0.2582912456973157	No Hit
GTCCTACAGTGTGCATTTCTCATTT	5544	0.2062163977744698	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3862	0.14365218762716492	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3656	0.1359897457185176	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3594	0.13368357388193441	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	3054	0.11359756111169385	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2697	0.10031847489136815	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.719631994488993E-5	0.0
4	0.0	0.0	0.0	3.719631994488993E-5	0.0
5	0.0	0.0	0.0	7.439263988977986E-5	0.0
6	0.0	0.0	0.0	7.439263988977986E-5	0.0
7	0.0	0.0	0.0	1.115889598346698E-4	0.0
8	0.0	0.0	0.0	1.115889598346698E-4	0.0
9	0.0	0.0	0.0	1.115889598346698E-4	0.0
10	0.0	0.0	0.0	1.115889598346698E-4	0.0
11	0.0	0.0	0.0	1.115889598346698E-4	0.0
12	0.0	0.0	0.0	1.115889598346698E-4	0.0
13	0.0	0.0	0.0	1.4878527977955973E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGAGT	25	0.0060377056	18.997541	8
TAGGACC	1380	0.0	13.835166	4
TCCAACG	235	0.0	12.934497	18
GGTATCA	2680	0.0	12.684623	1
TGTAGGA	4725	0.0	11.398526	2
GGCGAGG	1845	0.0	11.37772	19
GCCGGTT	160	4.5474735E-11	11.27958	11
CTACACG	110	4.981066E-7	11.2258215	4
CGATTTG	60	0.0058823917	11.082106	14
GTAGGAC	4630	0.0	11.078481	3
CTGTAGG	4840	0.0	11.076617	1
AGGACGT	4520	0.0	11.074895	5
GTCCTAC	4490	0.0	11.070526	1
GGACGTG	4475	0.0	11.037678	6
CCAACGA	185	5.456968E-12	10.782188	19
TAATACT	330	0.0	10.650138	4
GTCGAGG	135	5.7050784E-8	10.553993	19
GACGTGA	2310	0.0	10.526776	7
AGGACCT	2665	0.0	10.514587	5
TAGGACG	4905	0.0	10.496094	4
>>END_MODULE