FastQCFastQC Report
Thu 26 May 2016
SRR1512888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2688438
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT152550.5674298607592959No Hit
TATCAACGCAGAGTACTTTTTTTTT109610.40770886291593855No Hit
GTCCTACAGTGGACATTTCTAAATT94250.35057531548058757No Hit
GGTATCAACGCAGAGTACTTTTTTT84660.3149040446534382No Hit
CTGTAGGACGTGGAATATGGCAAGA83280.30977095250104336No Hit
GTCCTAAAGTGTGTATTTCTCATTT75150.27953034438584784No Hit
CTTTAGGACGTGAAATATGGCGAGG74420.2768150130298709No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53910.20052536082290165No Hit
GTCCTACAGTGTGCATTTCTCATTT51700.19230497411508096No Hit
CTGTAGGACCTGGAATATGGCGAGA40240.1496779914582371No Hit
CTGAAGGACCTGGAATATGGCGAGA37800.14060208939168395No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30760.11441588015048143No Hit
TTTCTAAATTTTCCACCTTTTTCAG28090.1044844627251958No Hit
GAGTACTTTTTTTTTTTTTTTTTTT27030.1005416528110375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG400.005285612314.2478929
TAGGACC14750.013.794364
TCCAACG2450.013.56210218
CCGTACA1800.012.65796317
ACCGTGC701.0908925E-412.2149798
GGTATCA26550.012.1079211
GCGAATC658.0681435E-411.685142515
CCCCGTA1950.011.68514215
GGCGAGG18650.011.6059619
AGGACCT27100.011.542895
CACCTTT11050.011.51330214
CCAACGA2200.011.21955719
TGTAGGA47350.011.0654242
CTGTAGG46900.010.9100511
GTAGGAC46450.010.8703943
TAGAACG1053.4347559E-610.8660524
GTATCAA58650.010.864861
ACCTCGA700.001491308110.8577598
ACCGTAT700.001491308110.8577598
GTACCGT1158.7288936E-710.7481436
Produced by FastQC (version 0.11.5)