##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512888_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2688438 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.398570842995078 31.0 31.0 34.0 26.0 34.0 2 30.420162934759887 31.0 30.0 34.0 26.0 34.0 3 30.582867077462826 31.0 31.0 34.0 27.0 34.0 4 34.165137153990536 37.0 35.0 37.0 30.0 37.0 5 33.77777951360604 37.0 35.0 37.0 28.0 37.0 6 33.631976262796464 37.0 35.0 37.0 28.0 37.0 7 33.47081093184965 37.0 35.0 37.0 28.0 37.0 8 33.41841173201688 37.0 35.0 37.0 27.0 37.0 9 34.73121827618863 39.0 35.0 39.0 27.0 39.0 10 34.668071571670986 39.0 35.0 39.0 27.0 39.0 11 34.63128143553989 39.0 35.0 39.0 27.0 39.0 12 34.499996652331205 39.0 35.0 39.0 27.0 39.0 13 34.491068791618034 39.0 35.0 39.0 27.0 39.0 14 35.5878789096122 40.0 36.0 41.0 27.0 41.0 15 35.59862343859148 40.0 36.0 41.0 27.0 41.0 16 35.61193376972056 40.0 36.0 41.0 27.0 41.0 17 35.61848032203086 40.0 36.0 41.0 27.0 41.0 18 35.55648633146831 40.0 36.0 41.0 27.0 41.0 19 35.578524407109256 40.0 36.0 41.0 26.0 41.0 20 35.558627723607536 40.0 36.0 41.0 26.0 41.0 21 35.52926643649584 40.0 36.0 41.0 26.0 41.0 22 35.49795940988783 40.0 36.0 41.0 26.0 41.0 23 35.34432410195065 40.0 36.0 41.0 25.0 41.0 24 35.3130405090242 40.0 36.0 41.0 25.0 41.0 25 35.25751905009526 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6572171674202387 1101 2 0.48008811512323746 1101 3 0.46322258448158493 1101 4 0.43643078241133537 1101 5 0.6321991477323863 1101 6 0.6574314400656078 1101 7 0.694936744762984 1101 8 0.510406086744176 1101 9 0.9041115215358957 1101 10 0.42535877563945945 1101 11 0.817826927143976 1101 12 0.87792076053627 1101 13 0.9454264911721992 1101 14 1.0902144176200395 1101 15 0.6394691763803095 1101 16 0.9168120419701467 1101 17 0.9102942543493526 1101 18 0.7848223414088267 1101 19 0.6623032151180297 1101 20 0.7884621828057377 1101 21 0.9136345130148555 1101 22 0.8457056762933419 1101 23 1.0046229264796551 1101 24 1.1055875611048336 1101 25 1.148145486118878 1102 1 0.858676324405824 1102 2 0.9314882732644705 1102 3 0.5885537687161211 1102 4 0.6971485003117053 1102 5 0.871393235683243 1102 6 0.8453975005242143 1102 7 0.8863442017303242 1102 8 0.9160930887513601 1102 9 0.992484491549888 1102 10 0.9184662508538608 1102 11 1.0756853299175333 1102 12 1.0748972218220842 1102 13 1.1416560392532134 1102 14 1.1695228538465443 1102 15 1.311621113610407 1102 16 1.208684190866201 1102 17 1.1893247269483496 1102 18 0.6645820883828506 1102 19 0.9631675177760215 1102 20 1.1942538013204285 1102 21 1.12313636813316 1102 22 0.8898447189306466 1102 23 1.1244678264249117 1102 24 1.111929024519469 1102 25 1.1009354623976932 1103 1 0.5623154795623364 1103 2 0.7130183553436531 1103 3 0.48288143161936503 1103 4 0.3349371493327098 1103 5 0.5644870254531185 1103 6 0.4962778377143735 1103 7 0.5873346394470502 1103 8 0.5852404226373338 1103 9 0.6777387268591113 1103 10 0.6437844682294269 1103 11 0.7455139327033251 1103 12 0.8002902795343729 1103 13 0.8779484653177363 1103 14 0.9526622950542674 1103 15 0.9751168928956275 1103 16 0.8944396579469611 1103 17 0.8462236983315492 1103 18 0.8784144740499329 1103 19 0.933951881866669 1103 20 0.8036668709417896 1103 21 1.0349814568090352 1103 22 0.8363576963150905 1103 23 0.8600113820998203 1103 24 0.8566167616792697 1103 25 0.8448311295975444 1104 1 0.7708279216793734 1104 2 0.8069322601718731 1104 3 0.7770352150024458 1104 4 0.5811518321199145 1104 5 0.6812477623670361 1104 6 0.7330281625451178 1104 7 0.633994103770469 1104 8 0.6778019309480072 1104 9 0.8259027625291324 1104 10 0.8537902222753928 1104 11 0.9111262665779805 1104 12 0.8541307886166223 1104 13 0.7485532341991927 1104 14 0.9886280756346935 1104 15 1.1403878753039933 1104 16 1.0276399505536702 1104 17 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0.8800586680773961 1107 17 0.9417763038989193 1107 18 0.9402313034256622 1107 19 1.0022514060300907 1107 20 1.0059605554985183 1107 21 0.915589296031925 1107 22 0.9034545650914225 1107 23 1.072947671966503 1107 24 1.1736235638742798 1107 25 1.0687054409513905 1108 1 0.6282795253789573 1108 2 0.5183567934620257 1108 3 0.5569175781219364 1108 4 0.4280636835341909 1108 5 0.6451480154614728 1108 6 0.6386421744856179 1108 7 0.5310596676566277 1108 8 0.47510159802370566 1108 9 0.7032050483975709 1108 10 0.6406766343061108 1108 11 0.6450144996580889 1108 12 0.7622167165594007 1108 13 0.7684227410232154 1108 14 0.8252841549599026 1108 15 0.8484359651345841 1108 16 0.836640501299776 1108 17 0.8619118047932659 1108 18 0.9127496945798086 1108 19 0.8716018341903009 1108 20 0.8921548099310712 1108 21 0.9051642893590355 1108 22 1.0227763913321084 1108 23 1.0015594123257614 1108 24 0.9821980436723692 1108 25 0.9762680335945646 1109 1 0.21094706179851386 1109 2 0.12421497745458865 1109 3 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2216 24 -2.74768346693255 2216 25 -2.8933693016779856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68504.0 3 0.0 4 1147.0 5 10082.0 6 13197.0 7 10515.0 8 8495.0 9 7313.0 10 5997.0 11 5254.0 12 4744.0 13 4331.0 14 4266.0 15 4260.0 16 4193.0 17 4481.0 18 5020.0 19 6208.0 20 8184.0 21 10915.0 22 13910.0 23 16870.0 24 19654.0 25 22555.0 26 24666.0 27 27037.0 28 30232.0 29 36263.0 30 45248.0 31 57326.0 32 73098.0 33 94113.0 34 122927.0 35 163210.0 36 225883.0 37 352458.0 38 1175882.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.15551544879612 19.81379394556699 13.836832002811445 26.193858602825443 2 12.627405683512189 20.840462593802958 42.30646430110424 24.225667421580617 3 17.313793770555232 26.113591380567584 29.78559310683513 26.787021742042054 4 10.889738569944377 17.34977378140845 38.34478592463943 33.41570172400774 5 11.768767461672674 39.763804719441374 35.566083711863335 12.901344107022613 6 30.525622344672037 38.03778823625743 18.495765432305536 12.940823986764999 7 27.453698395989452 32.17218881257361 22.240887454234407 18.133225337202532 8 24.733229906234616 38.412400236823 20.385235552178276 16.46913430476411 9 26.868979905706038 14.832195364766887 20.416878567041312 37.88194616248576 10 15.073468941203263 29.972633814840254 34.06948644305832 20.884410800898166 11 33.335007234974874 22.668943172899162 25.591538241212948 18.40451135091302 12 23.82128318663072 25.480093972992435 31.910756902164533 18.787865938212313 13 28.924291762731308 22.908766675073487 26.246348055681413 21.920593506513796 14 20.10444757721049 23.660500738349146 28.130953753677794 28.104097930762563 15 22.06256003297489 33.33239091017988 23.46150040214188 21.14354865470335 16 20.495379623770692 28.704140798995315 29.714481915600803 21.085997661633186 17 20.134954172122367 29.966559040590408 28.91054636352815 20.987940423759078 18 19.96206501991605 27.557728275732774 32.528594449098385 19.951612255252794 19 22.752941894149835 26.840162416065443 29.06639031931213 21.34050537047259 20 23.260848460231145 27.426487572826154 29.801411459431794 19.51125250751091 21 21.83407819047141 26.842419474527002 28.479059378411602 22.844442956589987 22 22.424165777052036 29.129268755370237 28.528992757854954 19.917572709722776 23 20.60817470962693 28.74044333549816 29.81106501247193 20.840316942402985 24 22.41044799991668 26.93210704505739 30.48041278988022 20.177032165145707 25 21.295711487488273 28.01857435432768 30.067161675292493 20.618552482891552 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 194.0 1 194.0 2 597.5 3 1001.0 4 1001.0 5 1001.0 6 2596.5 7 4192.0 8 4192.0 9 4192.0 10 5530.0 11 6868.0 12 6868.0 13 6868.0 14 9765.5 15 12663.0 16 12663.0 17 12663.0 18 23342.5 19 34022.0 20 34022.0 21 34022.0 22 55850.5 23 77679.0 24 77679.0 25 77679.0 26 115718.5 27 153758.0 28 153758.0 29 153758.0 30 197938.5 31 242119.0 32 242119.0 33 242119.0 34 266523.5 35 290928.0 36 290928.0 37 290928.0 38 304884.0 39 318840.0 40 318840.0 41 318840.0 42 330086.5 43 341333.0 44 341333.0 45 341333.0 46 351512.5 47 361692.0 48 361692.0 49 361692.0 50 337040.0 51 312388.0 52 312388.0 53 312388.0 54 264487.5 55 216587.0 56 216587.0 57 216587.0 58 180966.5 59 145346.0 60 145346.0 61 145346.0 62 117579.5 63 89813.0 64 89813.0 65 89813.0 66 68058.5 67 46304.0 68 46304.0 69 46304.0 70 33272.5 71 20241.0 72 20241.0 73 20241.0 74 14237.5 75 8234.0 76 8234.0 77 8234.0 78 5576.5 79 2919.0 80 2919.0 81 2919.0 82 1945.5 83 972.0 84 972.0 85 972.0 86 614.5 87 257.0 88 257.0 89 257.0 90 166.0 91 75.0 92 75.0 93 75.0 94 44.0 95 13.0 96 13.0 97 13.0 98 6.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0845844315546797 2 0.04299894585629276 3 0.020755546529248582 4 0.004054398873993003 5 0.01517609853751509 6 0.06036962727055636 7 0.07149132693407845 8 0.04459838761392303 9 0.01714750349459426 10 0.043891657534970124 11 0.004017202554048113 12 2.231779196693396E-4 13 0.0053562700720641504 14 2.9757055955911946E-4 15 0.012349178221703457 16 0.008815527826938913 17 0.004389165753497012 18 0.00572823327151305 19 0.0012646748781262576 20 5.579447991733489E-4 21 1.115889598346698E-4 22 2.9757055955911946E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2688438.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.3490184921601 #Duplication Level Percentage of deduplicated Percentage of total 1 76.74874547429984 43.2471647798141 2 13.889775045922601 15.653503818292727 3 4.3984673678348996 7.435479571418744 4 1.8466487906381006 4.162273874087656 5 0.9203089309009239 2.5929252482918126 6 0.5438613260808349 1.8387631152299806 7 0.3571039755183522 1.4085720964075261 8 0.23427420114213804 1.0560897033915497 9 0.16986244136430145 0.8614423664604434 >10 0.7907654804250662 7.956944994099172 >50 0.05524971226802092 2.15157572740695 >100 0.036943257236749655 4.106766236960782 >500 0.004360361655379636 1.6157146700921845 >1k 0.003039039941592582 3.010781409241499 >5k 4.624625998075668E-4 1.9260438673252234 >10k+ 1.3213217137359053E-4 0.9759585214797544 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 15255 0.5674298607592959 No Hit TATCAACGCAGAGTACTTTTTTTTT 10961 0.40770886291593855 No Hit GTCCTACAGTGGACATTTCTAAATT 9425 0.35057531548058757 No Hit GGTATCAACGCAGAGTACTTTTTTT 8466 0.3149040446534382 No Hit CTGTAGGACGTGGAATATGGCAAGA 8328 0.30977095250104336 No Hit GTCCTAAAGTGTGTATTTCTCATTT 7515 0.27953034438584784 No Hit CTTTAGGACGTGAAATATGGCGAGG 7442 0.2768150130298709 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 5391 0.20052536082290165 No Hit GTCCTACAGTGTGCATTTCTCATTT 5170 0.19230497411508096 No Hit CTGTAGGACCTGGAATATGGCGAGA 4024 0.1496779914582371 No Hit CTGAAGGACCTGGAATATGGCGAGA 3780 0.14060208939168395 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3076 0.11441588015048143 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2809 0.1044844627251958 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2703 0.1005416528110375 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.719631994488993E-5 0.0 10 0.0 0.0 0.0 7.439263988977986E-5 0.0 11 0.0 0.0 0.0 7.439263988977986E-5 0.0 12 0.0 0.0 0.0 7.439263988977986E-5 0.0 13 0.0 0.0 0.0 1.4878527977955973E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTGCG 40 0.0052856123 14.247892 9 TAGGACC 1475 0.0 13.79436 4 TCCAACG 245 0.0 13.562102 18 CCGTACA 180 0.0 12.657963 17 ACCGTGC 70 1.0908925E-4 12.214979 8 GGTATCA 2655 0.0 12.107921 1 GCGAATC 65 8.0681435E-4 11.6851425 15 CCCCGTA 195 0.0 11.685142 15 GGCGAGG 1865 0.0 11.60596 19 AGGACCT 2710 0.0 11.54289 5 CACCTTT 1105 0.0 11.513302 14 CCAACGA 220 0.0 11.219557 19 TGTAGGA 4735 0.0 11.065424 2 CTGTAGG 4690 0.0 10.910051 1 GTAGGAC 4645 0.0 10.870394 3 TAGAACG 105 3.4347559E-6 10.866052 4 GTATCAA 5865 0.0 10.86486 1 ACCTCGA 70 0.0014913081 10.857759 8 ACCGTAT 70 0.0014913081 10.857759 8 GTACCGT 115 8.7288936E-7 10.748143 6 >>END_MODULE