FastQCFastQC Report
Thu 26 May 2016
SRR1512887_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512887_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475897
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67890.27420365225209287No Hit
GTCCTACAGTGGACATTTCTAAATT52730.21297331835694297No Hit
GTCCTAAAGTGTGTATTTCTCATTT47040.189991748445109No Hit
TATCAACGCAGAGTACTTTTTTTTT38160.15412595919781802No Hit
GGTATCAACGCAGAGTACTTTTTTT37550.15166220565718203No Hit
CTGTAGGACGTGGAATATGGCAAGA37210.15028896597879474No Hit
GTCCTACAGTGTGCATTTCTCATTT33760.13635462218339456No Hit
CTTTAGGACGTGAAATATGGCGAGG33590.1356680023442009No Hit
GTACTGGTTCACTATCGGTCAGTCA33460.1351429401142293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19450.013.0693651
CTAACGT550.003072191312.0894044
TAGGACC9100.011.8996174
CTTAGAC1302.6229827E-911.6908523
GCGCCAC1552.5465852E-1111.64371113
ACCGTCC752.0763821E-411.3990418
TACCGTA752.0763821E-411.3990417
CCAACGA752.077552E-411.3983519
GCACGGC855.332336E-511.17507913
TAGGCCG600.00588277411.0819535
GTCCTAC25500.010.9766541
GTATCAA43300.010.971741
GGCGAGG9900.010.93781119
TCCTATA3350.010.7747772
TGTAGGA26600.010.7129522
CACCTTT8150.010.60604114
GCACCGT1355.698712E-810.5546676
TCCTACA28150.010.4942532
TCCAACG1553.6725396E-910.41805718
GTAGGAC26550.010.4111333
Produced by FastQC (version 0.11.5)