##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512887_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2475897 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.89815125588827 31.0 30.0 34.0 23.0 34.0 2 29.027646141984096 31.0 30.0 34.0 22.0 34.0 3 29.133770104329866 31.0 30.0 34.0 22.0 34.0 4 32.59208117300518 35.0 33.0 37.0 25.0 37.0 5 32.760970266533704 35.0 33.0 37.0 26.0 37.0 6 32.732899228037354 36.0 33.0 37.0 25.0 37.0 7 32.721740443968386 37.0 33.0 37.0 25.0 37.0 8 32.65653700456844 37.0 33.0 37.0 25.0 37.0 9 33.97959567784928 39.0 34.0 39.0 23.0 39.0 10 33.83047033055091 38.0 34.0 39.0 23.0 39.0 11 33.91725019255647 39.0 34.0 39.0 23.0 39.0 12 33.80040486336871 38.0 34.0 39.0 22.0 39.0 13 33.7827248064035 39.0 34.0 39.0 22.0 39.0 14 34.64021443541472 39.0 34.0 41.0 18.0 41.0 15 34.82091702522359 39.0 34.0 41.0 22.0 41.0 16 34.76903845353825 39.0 34.0 41.0 22.0 41.0 17 34.53014240899359 39.0 34.0 41.0 18.0 41.0 18 34.537656453398505 39.0 34.0 41.0 18.0 41.0 19 34.65851729696348 39.0 34.0 41.0 18.0 41.0 20 34.626860083436426 39.0 34.0 41.0 18.0 41.0 21 34.52730061064737 39.0 34.0 41.0 18.0 41.0 22 34.542562554096556 39.0 34.0 41.0 18.0 41.0 23 34.2189218695285 39.0 34.0 41.0 11.0 41.0 24 34.28835327156178 39.0 34.0 41.0 11.0 41.0 25 34.008247515950785 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7318734047015383 1101 2 0.28824560387637277 1101 3 0.6735531625170985 1101 4 0.784952216684303 1101 5 0.7124388477202572 1101 6 0.6826115158319936 1101 7 0.49532564631485343 1101 8 0.7667841173261891 1101 9 0.759493273732879 1101 10 0.8803669878986753 1101 11 0.8500123058414957 1101 12 0.6668297537816841 1101 13 1.1382187452558696 1101 14 1.227983296430395 1101 15 1.0613785695467826 1101 16 0.8891887212656471 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2214 5 -1.79978597514976 2214 6 -1.6204006499310246 2214 7 -1.4989480973744485 2214 8 -1.4085919183102718 2214 9 -1.2005072021450687 2214 10 -1.2887159868030338 2214 11 -1.2593612999848318 2214 12 -1.1930971309711609 2214 13 -1.526355852436069 2214 14 -1.363042806891201 2214 15 -1.7504747286131277 2214 16 -1.5300468601283228 2214 17 -3.6644062519049676 2214 18 -3.2929892792668767 2214 19 -2.205478135011738 2214 20 -2.1481582904912457 2214 21 -2.041940950131618 2214 22 -3.1047204097099126 2214 23 -1.9176664363006068 2214 24 -3.127819331090709 2214 25 -2.9808057537272923 2215 1 -1.6433486601388942 2215 2 -3.1483465142040536 2215 3 -1.9946780760948712 2215 4 -3.599594487642854 2215 5 -2.1225520418560784 2215 6 -1.9932248010562716 2215 7 -2.12774286885001 2215 8 -1.6514229490729342 2215 9 -4.200173305321098 2215 10 -2.2978801599191314 2215 11 -1.8822734908440708 2215 12 -1.805522235869276 2215 13 -1.959936414789187 2215 14 -1.595818637013224 2215 15 -1.8127561624778465 2215 16 -1.7919221391706657 2215 17 -2.173642246909324 2215 18 -1.8726539269253166 2215 19 -1.9876144096172084 2215 20 -1.785853388449759 2215 21 -2.3293560653819014 2215 22 -2.252356093113576 2215 23 -2.2067728411776244 2215 24 -2.117321478660571 2215 25 -3.073111190003697 2216 1 -2.8465225918030015 2216 2 -2.972314999470367 2216 3 -4.210324979461529 2216 4 -4.771197539881893 2216 5 -4.537549482654967 2216 6 -3.57080985532178 2216 7 -5.295104175818352 2216 8 -3.2811243059718116 2216 9 -3.965329782189052 2216 10 -2.9169926916480016 2216 11 -3.095944847638492 2216 12 -3.4579033374747823 2216 13 -2.9177866336269958 2216 14 -3.9031826076398595 2216 15 -3.404133482733137 2216 16 -3.6337629881986935 2216 17 -3.2580123178357567 2216 18 -3.0198651811427766 2216 19 -3.782294666164063 2216 20 -4.21466241981825 2216 21 -3.930733594551569 2216 22 -3.5972439583220215 2216 23 -3.6147444914967437 2216 24 -4.009963253179283 2216 25 -3.991931241605549 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 118239.0 3 4.0 4 1129.0 5 6319.0 6 7577.0 7 6867.0 8 6103.0 9 5733.0 10 5222.0 11 5001.0 12 4557.0 13 4526.0 14 4459.0 15 4651.0 16 4888.0 17 5113.0 18 5851.0 19 7093.0 20 9240.0 21 11637.0 22 14813.0 23 17703.0 24 20773.0 25 23390.0 26 26146.0 27 28912.0 28 32897.0 29 39172.0 30 47497.0 31 58974.0 32 74040.0 33 94389.0 34 122175.0 35 161277.0 36 219833.0 37 343042.0 38 926655.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4928900579956 18.70120007245904 12.590082432289499 24.21582743725586 2 14.817323709947667 20.04955494264908 42.36814297416906 22.76497837323419 3 19.332051795250663 25.867976803056624 28.759677679303913 26.040293722388796 4 12.053337900552307 16.50884043669184 38.6371779434023 32.80064371935355 5 12.924636147342653 39.12683441119087 34.89976126903082 13.048768172435663 6 31.195379234852517 36.559391012322365 19.034866743523118 13.210363009302004 7 28.54319099303248 31.809926565549958 21.840150013723232 17.80673242769433 8 25.475879820204984 36.60151516560525 20.812845874333522 17.109759139856244 9 28.01287578457517 14.87231540578812 20.044983589963746 37.069825219672964 10 15.891391388154021 28.904284254306244 33.71849147159462 21.48583288594511 11 34.45885090405767 22.580178321373015 24.628254591435788 18.332716183133527 12 24.014523995885863 24.949381909450217 31.211551274670256 19.82454281999366 13 29.153645185922695 22.236545682608558 25.88826003103731 22.721549100431442 14 21.290932089205608 22.512300395237745 27.799641104563566 28.397126410993085 15 23.319153890278358 31.255141446109157 23.429916051175354 21.995788612437135 16 22.026205194792595 28.304391625850744 27.874429803355934 21.794973376000723 17 21.60153312471193 28.74798858242567 27.670070915104027 21.98040737775837 18 21.29584967939945 27.318061865657068 30.177974849261744 21.20811360568174 19 23.323110335604333 26.962758964191746 27.950869776851338 21.763260923352583 20 24.10586505948502 26.864839559437314 28.30090509079207 20.728390290285596 21 23.693901342681222 26.636999081595697 27.038235046825683 22.630864528897398 22 23.552488843838812 28.250347612923154 27.163296603683264 21.03386693955477 23 22.372355375442492 27.827158836761395 27.973428830632447 21.82705695716367 24 22.99554741273713 26.80838350949904 28.64520714192733 21.550861935836497 25 22.562928136704592 27.50710992438315 28.249331454871434 21.680630484040822 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 290.0 1 290.0 2 743.0 3 1196.0 4 1196.0 5 1196.0 6 1977.5 7 2759.0 8 2759.0 9 2759.0 10 3243.0 11 3727.0 12 3727.0 13 3727.0 14 5689.0 15 7651.0 16 7651.0 17 7651.0 18 15728.5 19 23806.0 20 23806.0 21 23806.0 22 40577.5 23 57349.0 24 57349.0 25 57349.0 26 86705.5 27 116062.0 28 116062.0 29 116062.0 30 151933.5 31 187805.0 32 187805.0 33 187805.0 34 214859.5 35 241914.0 36 241914.0 37 241914.0 38 260532.5 39 279151.0 40 279151.0 41 279151.0 42 295716.0 43 312281.0 44 312281.0 45 312281.0 46 326561.5 47 340842.0 48 340842.0 49 340842.0 50 325706.5 51 310571.0 52 310571.0 53 310571.0 54 271347.5 55 232124.0 56 232124.0 57 232124.0 58 196915.0 59 161706.0 60 161706.0 61 161706.0 62 132403.5 63 103101.0 64 103101.0 65 103101.0 66 78379.5 67 53658.0 68 53658.0 69 53658.0 70 39382.5 71 25107.0 72 25107.0 73 25107.0 74 17435.5 75 9764.0 76 9764.0 77 9764.0 78 6687.5 79 3611.0 80 3611.0 81 3611.0 82 2312.5 83 1014.0 84 1014.0 85 1014.0 86 665.5 87 317.0 88 317.0 89 317.0 90 191.0 91 65.0 92 65.0 93 65.0 94 41.5 95 18.0 96 18.0 97 18.0 98 13.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3350704815264932 2 0.010541634001737552 3 0.009128004921044775 4 0.011470590254764233 5 0.012399546507790914 6 0.08162698205943139 7 0.08037491058796065 8 0.10646646447731872 9 0.08506008125539956 10 0.09212822665886343 11 0.08728149838220249 12 0.09988299190152096 13 0.08481774484156651 14 0.08869512746289526 15 0.08594864810612073 16 0.08841240164675672 17 0.10166012560296328 18 0.10380076392515522 19 0.09649028210785829 20 0.09422847557874983 21 0.08243477010554154 22 0.10501244599432043 23 0.09649028210785829 24 0.09204744785425242 25 0.09055303996894862 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2475897.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.745374615098605 #Duplication Level Percentage of deduplicated Percentage of total 1 73.98002478228202 40.50064170740308 2 14.3107137077531 15.668907658807404 3 5.166198958098797 8.484764918917524 4 2.407481005112979 5.271937980145767 5 1.2839090942925742 3.5144042169389467 6 0.750243695390259 2.4643423294053393 7 0.47375347117432126 1.8155067878249074 8 0.3279040801101989 1.4360985362761713 9 0.2360130776063527 1.1628561912859907 >10 0.9639287834348207 8.86020022751147 >50 0.053531763159157864 2.029462876238638 >100 0.03918418202993125 4.114603803806032 >500 0.0045199726342317085 1.7345670765479844 >1k 0.002445231097203678 2.45240849344408 >5k 1.4819582407295018E-4 0.48929719544664707 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6789 0.27420365225209287 No Hit GTCCTACAGTGGACATTTCTAAATT 5273 0.21297331835694297 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4704 0.189991748445109 No Hit TATCAACGCAGAGTACTTTTTTTTT 3816 0.15412595919781802 No Hit GGTATCAACGCAGAGTACTTTTTTT 3755 0.15166220565718203 No Hit CTGTAGGACGTGGAATATGGCAAGA 3721 0.15028896597879474 No Hit GTCCTACAGTGTGCATTTCTCATTT 3376 0.13635462218339456 No Hit CTTTAGGACGTGAAATATGGCGAGG 3359 0.1356680023442009 No Hit GTACTGGTTCACTATCGGTCAGTCA 3346 0.1351429401142293 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1945 0.0 13.069365 1 CTAACGT 55 0.0030721913 12.089404 4 TAGGACC 910 0.0 11.899617 4 CTTAGAC 130 2.6229827E-9 11.690852 3 GCGCCAC 155 2.5465852E-11 11.643711 13 ACCGTCC 75 2.0763821E-4 11.399041 8 TACCGTA 75 2.0763821E-4 11.399041 7 CCAACGA 75 2.077552E-4 11.39835 19 GCACGGC 85 5.332336E-5 11.175079 13 TAGGCCG 60 0.005882774 11.081953 5 GTCCTAC 2550 0.0 10.976654 1 GTATCAA 4330 0.0 10.97174 1 GGCGAGG 990 0.0 10.937811 19 TCCTATA 335 0.0 10.774777 2 TGTAGGA 2660 0.0 10.712952 2 CACCTTT 815 0.0 10.606041 14 GCACCGT 135 5.698712E-8 10.554667 6 TCCTACA 2815 0.0 10.494253 2 TCCAACG 155 3.6725396E-9 10.418057 18 GTAGGAC 2655 0.0 10.411133 3 >>END_MODULE