FastQCFastQC Report
Thu 26 May 2016
SRR1512887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475897
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81500.3291736287898891No Hit
TATCAACGCAGAGTACTTTTTTTTT56310.2274327243823148No Hit
GTCCTACAGTGGACATTTCTAAATT48460.1957270435724911No Hit
GGTATCAACGCAGAGTACTTTTTTT46140.18635670223761328No Hit
GTCCTAAAGTGTGTATTTCTCATTT42180.17036249892463218No Hit
CTGTAGGACGTGGAATATGGCAAGA37340.15081402820876635No Hit
CTTTAGGACGTGAAATATGGCGAGG36290.14657314096668803No Hit
GTCCTACAGTGTGCATTTCTCATTT31550.1274285642738773No Hit
GTACTGGTTCACTATCGGTCAGTCA30370.12266261480182739No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27750.11208059139778431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.014.0592611
TAGGACC9600.013.3723634
GGCGAGG10100.012.3120919
TCGAACT2950.012.22766219
TACGCTG802.8395898E-511.8865455
CTCGAAC3050.011.82699418
CCAACGA1052.750112E-711.75265319
TGGCGAG23700.011.37524118
GCCTCGA2900.011.12984416
ACCCGAA600.005852566611.0896217
AGGACCT18500.011.0512735
TAGACTC2250.010.988455
GGACCTG17450.010.8988386
AAGTCGT1408.285497E-910.8633027
TAAGGCG700.001501009610.8492616
TGTAGGA25700.010.6211972
CTAGGAC4150.010.5422773
GTATCAA42900.010.532071
ATGGCGA26800.010.41365417
TATGGCG26550.010.40486916
Produced by FastQC (version 0.11.5)