##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512887_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2475897 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.304242462428768 31.0 30.0 34.0 26.0 34.0 2 30.326314866894705 31.0 30.0 34.0 26.0 34.0 3 30.5083511147677 31.0 31.0 34.0 27.0 34.0 4 34.096195439471025 37.0 35.0 37.0 30.0 37.0 5 33.67912033497355 37.0 35.0 37.0 28.0 37.0 6 33.543774236165724 37.0 35.0 37.0 28.0 37.0 7 33.386647344376605 37.0 35.0 37.0 27.0 37.0 8 33.33452805185353 37.0 35.0 37.0 27.0 37.0 9 34.6722242484239 39.0 35.0 39.0 27.0 39.0 10 34.60389911211977 39.0 35.0 39.0 27.0 39.0 11 34.57980158302223 39.0 35.0 39.0 27.0 39.0 12 34.440908083009916 39.0 35.0 39.0 26.0 39.0 13 34.42734168666952 39.0 35.0 39.0 27.0 39.0 14 35.50942264561086 40.0 36.0 41.0 26.0 41.0 15 35.51309606175055 40.0 36.0 41.0 27.0 41.0 16 35.5123419916095 40.0 36.0 41.0 27.0 41.0 17 35.500796277066456 40.0 36.0 41.0 26.0 41.0 18 35.42636628260384 40.0 36.0 41.0 26.0 41.0 19 35.443782596771996 40.0 36.0 41.0 25.0 41.0 20 35.416108989994335 40.0 36.0 41.0 25.0 41.0 21 35.381819195225006 40.0 36.0 41.0 25.0 41.0 22 35.344160924303395 40.0 36.0 41.0 25.0 41.0 23 35.19031728702769 40.0 36.0 41.0 25.0 41.0 24 35.14308268881945 40.0 36.0 41.0 25.0 41.0 25 35.08303414883576 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6591085417332287 1101 2 0.5788341806457424 1101 3 0.5148726654644022 1101 4 0.5633072095607119 1101 5 0.6339142981622246 1101 6 0.6983600202016049 1101 7 0.5975689470725598 1101 8 0.4597540463687224 1101 9 0.791296956681542 1101 10 0.5719084443790692 1101 11 0.7857102401626577 1101 12 0.9129300383067545 1101 13 0.9913866315993616 1101 14 1.1112769259809951 1101 15 0.6380624127130687 1101 16 0.8572234519862576 1101 17 1.0029747027672613 1101 18 0.9045782584869926 1101 19 0.8443943484733225 1101 20 0.9265496278871268 1101 21 0.95269248103412 1101 22 0.8319940270849173 1101 23 0.8960332836145639 1101 24 0.9429310606472541 1101 25 1.0600054289785987 1102 1 0.7451483951395304 1102 2 0.8572340420701181 1102 3 0.5787210475602969 1102 4 0.6595983183173075 1102 5 0.8294957121838564 1102 6 0.7421806310236434 1102 7 0.9075930237450862 1102 8 0.95389995616992 1102 9 1.0576711153596108 1102 10 0.850803118482105 1102 11 1.0581272899055847 1102 12 1.025260798333413 1102 13 1.0820864272733033 1102 14 1.0968456615682598 1102 15 1.1529364116473673 1102 16 1.3061530282806117 1102 17 1.2261206221137755 1102 18 0.6370456064650938 1102 19 0.9494510111927212 1102 20 1.11933356052635 1102 21 1.0520806258557585 1102 22 0.8089016457683726 1102 23 0.9835754023505174 1102 24 0.9631743035414644 1102 25 1.086776268804158 1103 1 0.8386718491889482 1103 2 0.9762231414362468 1103 3 0.6620834519612089 1103 4 0.5278769045282701 1103 5 0.79570171424119 1103 6 0.842277805641146 1103 7 0.9051471914790454 1103 8 0.9904506231781198 1103 9 1.056305588360111 1103 10 1.0096295418463015 1103 11 1.1506468772997138 1103 12 1.3092550410422703 1103 13 1.2824480876175244 1103 14 1.2899757413815536 1103 15 1.255143169959048 1103 16 1.3158971215682485 1103 17 1.3919499200576553 1103 18 1.2840699895689056 1103 19 1.2623711694369106 1103 20 1.2164449535387192 1103 21 1.3424213062407162 1103 22 1.2612848634273135 1103 23 1.3264098924277263 1103 24 1.2850359496550894 1103 25 1.2497655470691598 1104 1 0.6825067102957121 1104 2 0.7625274108872091 1104 3 0.6043004587371392 1104 4 0.553902407925996 1104 5 0.6249230698779655 1104 6 0.5850242565175989 1104 7 0.48589451960074825 1104 8 0.6436229284850441 1104 9 0.7081348938047256 1104 10 0.7164191036377261 1104 11 0.7422558211617414 1104 12 0.780958091842713 1104 13 0.699891264443707 1104 14 0.8818800401862319 1104 15 0.9028299658022618 1104 16 0.9441559984586902 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2216 19 -3.018295418622678 2216 20 -2.2558057637509066 2216 21 -2.9929646545343473 2216 22 -2.2683362504350413 2216 23 -3.3281365447062576 2216 24 -2.5723708026932997 2216 25 -2.6023303142679453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67078.0 3 2.0 4 1108.0 5 9977.0 6 12066.0 7 9043.0 8 7393.0 9 6454.0 10 5337.0 11 4933.0 12 4431.0 13 4118.0 14 4010.0 15 3998.0 16 4255.0 17 4282.0 18 4946.0 19 5685.0 20 7528.0 21 10037.0 22 12498.0 23 15249.0 24 18127.0 25 20756.0 26 23151.0 27 25150.0 28 28587.0 29 34183.0 30 43038.0 31 54474.0 32 69721.0 33 89656.0 34 116716.0 35 153388.0 36 210764.0 37 327006.0 38 1056752.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.472662931145656 19.39404033343992 13.577032892446992 26.556263842967436 2 13.198994499324588 20.539828276130432 41.29797837147197 24.963198853073013 3 17.232851256362608 25.841560959844873 29.54189221943928 27.383695564353232 4 11.288420277117154 17.124731801261504 37.781972197807875 33.80487572381347 5 12.508897357818 39.15613055221386 35.08297871824724 13.251993371720905 6 31.248138423306678 37.0832543573524 18.511175118140656 13.157432101200259 7 28.099864712304473 31.942851761145068 21.893122316325833 18.06416121022463 8 25.43024092198524 37.02803617466642 20.387932079558404 17.153790823789937 9 27.270274037072873 14.738051906867389 20.06047519688777 37.93119885917197 10 15.360581498203224 29.449256903129296 33.65083842113281 21.53932317753467 11 34.2136672907162 22.65053427930357 24.53354821640988 18.60225021357035 12 23.582467044120772 25.210086409471195 31.302895205998983 19.90455134040905 13 28.88613237513289 22.141878508025037 26.04691423050451 22.925074886337566 14 20.939432015861843 22.432962192417317 27.92551655391883 28.70208923780201 15 23.08492443271009 31.27379781882109 23.430545714837397 22.210732033631423 16 21.908348443788096 28.32264055752428 27.762506871907643 22.00650412677998 17 21.291235422799403 28.825535668701306 27.806060625644243 22.077168282855048 18 21.09094625123043 27.35613603747376 30.363213866939393 21.189703844356416 19 23.108355961934674 26.851960680962367 28.14258866352512 21.89709469357784 20 23.868781478833704 26.80484031875664 28.446323918460987 20.880054283948674 21 23.345380199249885 26.446007611012785 27.36705163323352 22.84156055650381 22 23.292557275571806 28.063222747781495 27.425510353688242 21.218709622958453 23 22.117963711737605 27.643920566970277 28.18828893124391 22.049826790048215 24 22.78899324164131 26.755111379835267 28.809154823484175 21.646740555039244 25 22.298289022066374 27.35577532802293 28.520243596858347 21.82569205305235 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 111.0 1 111.0 2 353.0 3 595.0 4 595.0 5 595.0 6 1474.0 7 2353.0 8 2353.0 9 2353.0 10 2883.5 11 3414.0 12 3414.0 13 3414.0 14 5558.5 15 7703.0 16 7703.0 17 7703.0 18 15967.5 19 24232.0 20 24232.0 21 24232.0 22 41713.0 23 59194.0 24 59194.0 25 59194.0 26 89495.5 27 119797.0 28 119797.0 29 119797.0 30 154936.0 31 190075.0 32 190075.0 33 190075.0 34 216748.5 35 243422.0 36 243422.0 37 243422.0 38 262122.0 39 280822.0 40 280822.0 41 280822.0 42 296575.0 43 312328.0 44 312328.0 45 312328.0 46 326492.5 47 340657.0 48 340657.0 49 340657.0 50 324817.0 51 308977.0 52 308977.0 53 308977.0 54 269699.0 55 230421.0 56 230421.0 57 230421.0 58 195576.0 59 160731.0 60 160731.0 61 160731.0 62 131191.0 63 101651.0 64 101651.0 65 101651.0 66 76634.5 67 51618.0 68 51618.0 69 51618.0 70 37859.5 71 24101.0 72 24101.0 73 24101.0 74 16656.5 75 9212.0 76 9212.0 77 9212.0 78 6247.5 79 3283.0 80 3283.0 81 3283.0 82 2086.0 83 889.0 84 889.0 85 889.0 86 574.5 87 260.0 88 260.0 89 260.0 90 153.0 91 46.0 92 46.0 93 46.0 94 25.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08756422419834105 2 0.044832236559113725 3 0.02007353294583741 4 0.0048871176789664515 5 0.017892505221339984 6 0.06123033389514992 7 0.07714375840352002 8 0.04996169065191323 9 0.020275479957364947 10 0.04790183113433232 11 0.005331401104327038 12 4.4428342536058645E-4 13 0.00585646333429864 14 7.270092414991416E-4 15 0.01433823781845529 16 0.009612677748710871 17 0.004564002460522388 18 0.005573737518160086 19 0.0019386913106643775 20 0.0010905138622487123 21 4.4428342536058645E-4 22 6.462304368881257E-4 23 0.0 24 0.0 25 1.2116820691652359E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2475897.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.45099652124192 #Duplication Level Percentage of deduplicated Percentage of total 1 72.71596029728454 38.86740540888922 2 14.953232672153375 15.985303750811822 3 5.457025078245345 8.750502854208657 4 2.5218553437788365 5.391827248295918 5 1.3692138517549755 3.6592922413495725 6 0.7830849069482443 2.51140011822766 7 0.506291453154075 1.8943247890891086 8 0.3373879517073341 1.4426977786414124 9 0.24140443755599744 1.1612976976816185 >10 1.0092154557797262 9.12771250336433 >50 0.05864313996665972 2.191010950386661 >100 0.03926697345200102 4.0955660480538665 >500 0.004844694347098656 1.8485655548509832 >1k 0.0024223471735447064 2.5154928216982313 >5k 1.5139669834654415E-4 0.5576002344510749 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8150 0.3291736287898891 No Hit TATCAACGCAGAGTACTTTTTTTTT 5631 0.2274327243823148 No Hit GTCCTACAGTGGACATTTCTAAATT 4846 0.1957270435724911 No Hit GGTATCAACGCAGAGTACTTTTTTT 4614 0.18635670223761328 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4218 0.17036249892463218 No Hit CTGTAGGACGTGGAATATGGCAAGA 3734 0.15081402820876635 No Hit CTTTAGGACGTGAAATATGGCGAGG 3629 0.14657314096668803 No Hit GTCCTACAGTGTGCATTTCTCATTT 3155 0.1274285642738773 No Hit GTACTGGTTCACTATCGGTCAGTCA 3037 0.12266261480182739 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2775 0.11208059139778431 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1935 0.0 14.059261 1 TAGGACC 960 0.0 13.372363 4 GGCGAGG 1010 0.0 12.31209 19 TCGAACT 295 0.0 12.227662 19 TACGCTG 80 2.8395898E-5 11.886545 5 CTCGAAC 305 0.0 11.826994 18 CCAACGA 105 2.750112E-7 11.752653 19 TGGCGAG 2370 0.0 11.375241 18 GCCTCGA 290 0.0 11.129844 16 ACCCGAA 60 0.0058525666 11.089621 7 AGGACCT 1850 0.0 11.051273 5 TAGACTC 225 0.0 10.98845 5 GGACCTG 1745 0.0 10.898838 6 AAGTCGT 140 8.285497E-9 10.863302 7 TAAGGCG 70 0.0015010096 10.84926 16 TGTAGGA 2570 0.0 10.621197 2 CTAGGAC 415 0.0 10.542277 3 GTATCAA 4290 0.0 10.53207 1 ATGGCGA 2680 0.0 10.413654 17 TATGGCG 2655 0.0 10.404869 16 >>END_MODULE