FastQCFastQC Report
Thu 26 May 2016
SRR1512886_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512886_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843530
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54160.2937842074715356No Hit
GTCCTACAGTGGACATTTCTAAATT45220.2452902854849121No Hit
GTCCTAAAGTGTGTATTTCTCATTT41930.22744408824374976No Hit
CTGTAGGACGTGGAATATGGCAAGA38960.21133369134215338No Hit
CTTTAGGACGTGAAATATGGCGAGG32950.1787331912146805No Hit
TATCAACGCAGAGTACTTTTTTTTT31250.16951175191073647No Hit
GGTATCAACGCAGAGTACTTTTTTT30410.16495527601937587No Hit
GTACTGGTTCACTATCGGTCAGTCA25940.14070831502606412No Hit
GTACATGGGAAGCAGTGGTATCAAC21680.11760047300559252No Hit
GTCCTACAGTGTGCATTTCTCATTT21660.11749198548436966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTTCG456.769987E-414.77581414
TAGGACC6300.012.6646394
GGCGAGG9900.011.70519819
TAGAAAT5150.011.6194994
ATTTAGA5250.011.4295131
GCGTGCG1604.5474735E-1111.2794459
GTCGAGG951.3628778E-510.998239519
AAATGTC5100.010.9887367
TGTAGGA20850.010.9335022
GAACCGC700.001493283810.85570056
TGTCGAG1053.4722689E-610.855700518
CAGTCCG700.00149362210.8554059
AATGTCC5200.010.7774148
GTATCAA38100.010.7745421
GTCCTAC20350.010.7180941
GCTCTAT803.682339E-410.7151691
CGTGCGC1605.456968E-1010.6857910
GACAGTC1252.244451E-710.6385867
TAATACC909.531801E-510.5538664
AGGACCT13350.010.530155
Produced by FastQC (version 0.11.5)