##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512886_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1843530
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.370566250617024	31.0	28.0	33.0	16.0	34.0
2	28.504259762520817	31.0	28.0	34.0	16.0	34.0
3	28.619942176151188	31.0	28.0	34.0	16.0	34.0
4	32.06228105862123	35.0	32.0	37.0	19.0	37.0
5	32.217297792821384	35.0	33.0	37.0	22.0	37.0
6	32.208266749117186	35.0	33.0	37.0	22.0	37.0
7	32.18892667870878	35.0	33.0	37.0	21.0	37.0
8	32.12003710273226	35.0	33.0	37.0	19.0	37.0
9	33.37883788167266	38.0	33.0	39.0	18.0	39.0
10	33.232452414661005	38.0	33.0	39.0	17.0	39.0
11	33.290383123681195	38.0	33.0	39.0	17.0	39.0
12	33.173666281535965	38.0	33.0	39.0	17.0	39.0
13	33.143038626981934	38.0	33.0	39.0	17.0	39.0
14	33.95151204482704	39.0	33.0	41.0	16.0	41.0
15	34.11176926873986	39.0	33.0	41.0	17.0	41.0
16	34.045153048770565	39.0	33.0	41.0	17.0	41.0
17	33.8085960087441	39.0	33.0	41.0	11.0	41.0
18	33.791294961297076	39.0	33.0	41.0	11.0	41.0
19	33.89454850205855	39.0	33.0	41.0	11.0	41.0
20	33.87194349969895	39.0	33.0	41.0	10.0	41.0
21	33.7742548263386	39.0	33.0	41.0	10.0	41.0
22	33.76418881168193	39.0	33.0	41.0	2.0	41.0
23	33.41970133385407	39.0	33.0	41.0	2.0	41.0
24	33.46609602230503	39.0	33.0	41.0	2.0	41.0
25	33.16543750305121	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7388371070045672
1101	2	0.4395530664112428
1101	3	0.8323990375308163
1101	4	0.9659479231157775
1101	5	0.8079590097021239
1101	6	0.6939741466064575
1101	7	0.4277502442769432
1101	8	0.7658568903574974
1101	9	0.990366982122282
1101	10	0.9536029427861479
1101	11	0.8946716790515055
1101	12	0.8645549540271205
1101	13	1.1887582362527311
1101	14	1.2931649048610439
1101	15	1.0640319587507676
1101	16	0.9214832641319219
1101	17	0.18530595173397302
1101	18	0.6712923964004531
1101	19	0.1820205295384838
1101	20	-0.39599741156433055
1101	21	0.7684527187000185
1101	22	0.8493966720271189
1101	23	0.2737957734594616
1101	24	0.8497964659201713
1101	25	0.5054525366936815
1102	1	1.1168207449389413
1102	2	1.04469988839465
1102	3	1.0444242155250656
1102	4	1.2744685367490973
1102	5	1.1244659749373938
1102	6	1.0066020334274342
1102	7	1.0854597133476531
1102	8	1.0830692695224826
1102	9	1.431961454991189
1102	10	1.4633939065989452
1102	11	1.1633225580377555
1102	12	1.3271566118313132
1102	13	1.3326216005302527
1102	14	1.4503212837951622
1102	15	1.4931543828611638
1102	16	1.5829286807799718
1102	17	1.663310000358429
1102	18	1.7321888655605235
1102	19	1.4574437080764042
1102	20	1.6578909994459892
1102	21	1.7253471798391828
1102	22	1.4026029424145392
1102	23	1.4204982484360045
1102	24	0.6491264506901189
1102	25	1.0937172510091884
1103	1	0.7965311291787884
1103	2	0.8884063634678796
1103	3	1.0616861117401015
1103	4	1.1048518971247177
1103	5	0.9544163295982386
1103	6	0.6712137551837927
1103	7	0.4677943365743431
1103	8	0.7973124204041113
1103	9	1.0923207465310227
1103	10	1.1747110157540774
1103	11	0.9513162294236608
1103	12	1.1079237992415685
1103	13	1.2359204310109817
1103	14	1.378526244021458
1103	15	1.2481094938542157
1103	16	1.2580115127540097
1103	17	0.8741380149756282
1103	18	1.1761197856685044
1103	19	1.1633044965147477
1103	20	1.2698622388923155
1103	21	1.109755294680376
1103	22	1.151823083417355
1103	23	0.48156641295842917
1103	24	0.7579311395362538
1103	25	1.1247761002998615
1104	1	0.900751585480144
1104	2	0.9909951978036275
1104	3	0.8795407898972947
1104	4	1.0452330929777105
1104	5	0.8843227230205102
1104	6	0.7923154005378805
1104	7	0.9493615930496091
1104	8	0.7116999786614429
1104	9	1.091475979632719
1104	10	1.0801371568913538
1104	11	0.7105541536003983
1104	12	1.1535982461287446
1104	13	1.22203760507157
1104	14	1.4906872020586306
1104	15	1.1036194755286317
1104	16	1.1169155883868598
1104	17	1.4666934052289733
1104	18	1.3053765794501757
1104	19	0.8208224831836191
1104	20	0.9472715136475429
1104	21	1.0776118154867333
1104	22	0.9542895570738281
1104	23	1.2530062941163322
1104	24	1.118065183488703
1104	25	0.5947693263705389
1105	1	0.3865774496989225
1105	2	0.5094916796572058
1105	3	0.6386941406594495
1105	4	0.7117337935907599
1105	5	0.40064124442479
1105	6	0.7410924347402812
1105	7	0.7699056879697181
1105	8	0.6339719442209315
1105	9	0.30616816947866
1105	10	0.5672828970660078
1105	11	0.5645026687509613
1105	12	0.776561974198188
1105	13	0.7660377450951117
1105	14	0.8102149704764372
1105	15	0.7735310482428446
1105	16	0.6987446502500774
1105	17	1.0553079945147346
1105	18	0.9457022354499927
1105	19	0.6388967918696906
1105	20	0.8116055481626816
1105	21	0.7072201667899378
1105	22	0.7445328666842528
1105	23	0.8773015667697948
1105	24	1.0759720385545393
1105	25	1.254719083019097
1106	1	0.670681396601001
1106	2	0.7599408386498467
1106	3	0.5467739409524626
1106	4	0.6412842577672464
1106	5	0.6016759462005012
1106	6	0.7079173815240694
1106	7	0.6260014458239951
1106	8	0.715243438492493
1106	9	0.9493299544573972
1106	10	0.627434470039475
1106	11	0.4921562688627148
1106	12	0.6960189452962666
1106	13	0.8756175998502158
1106	14	1.0478920154891043
1106	15	0.5763920865055567
1106	16	0.7923066285051519
1106	17	0.9454293507803442
1106	18	0.8532645064736712
1106	19	0.9057564895955821
1106	20	0.9584588821292712
1106	21	1.0134746339917697
1106	22	1.0243515329905932
1106	23	1.3220187490648527
1106	24	0.9821648972526589
1106	25	1.1604941945883525
1107	1	0.7867154066814273
1107	2	0.7964690780035149
1107	3	0.7529035605533245
1107	4	0.7917884090282072
1107	5	0.7461898921776111
1107	6	0.9105398901506163
1107	7	0.7007241431501257
1107	8	0.8459217959619707
1107	9	0.7466376412706381
1107	10	0.8765611013558114
1107	11	0.7993132167865653
1107	12	0.912430938953463
1107	13	0.6765687600344776
1107	14	1.0315217097646112
1107	15	0.712958377461355
1107	16	0.9528064681169965
1107	17	1.1328490755360932
1107	18	1.0804014026317574
1107	19	0.8476687059780303
1107	20	1.1700563091656448
1107	21	0.8699892500926651
1107	22	0.953963211845462
1107	23	1.245734052951697
1107	24	1.0654849190187008
1107	25	1.2268283318104807
1108	1	0.4832599407545679
1108	2	0.466127949035382
1108	3	0.47611655779378026
1108	4	0.6214543777101937
1108	5	0.4034021060259789
1108	6	0.5228425327736232
1108	7	0.40462549464872666
1108	8	0.3475143496140163
1108	9	0.3803974226276736
1108	10	0.4324580411200287
1108	11	0.2799890544418062
1108	12	0.41572722314219845
1108	13	0.34560327346257935
1108	14	0.2599327785646963
1108	15	0.3808295830899979
1108	16	0.12280844132263269
1108	17	0.45159099496451205
1108	18	0.6342095422522576
1108	19	0.5269491632905243
1108	20	0.5744589137970877
1108	21	0.45787568180060845
1108	22	0.49803343440538583
1108	23	0.7861840321888636
1108	24	0.6826417947568117
1108	25	0.8630097076943741
1109	1	0.6471972021812746
1109	2	0.5731492229147044
1109	3	0.6387117631485282
1109	4	0.7629868450280881
1109	5	0.7046042702670832
1109	6	0.795190974260187
1109	7	0.6390528323813385
1109	8	0.6424407468130511
1109	9	0.6892304990259532
1109	10	0.8659304580536897
1109	11	0.7260369305138923
1109	12	0.5296695968058387
1109	13	0.7102195987394637
1109	14	0.9478882732430876
1109	15	0.742523082116314
1109	16	0.246639801661523
1109	17	0.9005960292036193
1109	18	0.8400582417109774
1109	19	0.7416564627816697
1109	20	0.5626882162379871
1109	21	0.6588951680599564
1109	22	0.6795880114774775
1109	23	1.015065048816993
1109	24	0.6753444386255083
1109	25	0.19844851732958801
1110	1	0.24668656544776724
1110	2	0.22545916964420698
1110	3	0.1786800254864147
1110	4	0.0961370778347046
1110	5	0.0661298838043507
1110	6	0.009053636254726882
1110	7	-0.020740867471516822
1110	8	0.044472756561120264
1110	9	0.20683363761121143
1110	10	0.11829638224772054
1110	11	0.043874473396016356
1110	12	0.2088947866221318
1110	13	0.2420585182953161
1110	14	0.1993009347727508
1110	15	0.13983964771612278
1110	16	0.21257914957642754
1110	17	0.17900280241271105
1110	18	-0.5501672038404593
1110	19	0.0436495405130799
1110	20	0.20254921515252988
1110	21	0.24865836671359176
1110	22	0.33189966809516847
1110	23	0.41158277602933424
1110	24	0.2652647332563447
1110	25	0.4891633863142104
1111	1	0.25130326146471305
1111	2	0.27864182947073246
1111	3	0.3474068202602929
1111	4	0.28894850150131646
1111	5	0.27208157432964697
1111	6	0.02835837820759224
1111	7	0.1147945330403175
1111	8	0.2264375386009334
1111	9	0.39686466761558137
1111	10	0.36907110769490004
1111	11	0.032675240145273676
1111	12	-0.4055354327558689
1111	13	0.08015769889999547
1111	14	0.14863808085581098
1111	15	0.12476255375708689
1111	16	0.4183965554361322
1111	17	-0.09459610967656573
1111	18	0.18176501012322177
1111	19	0.149023957979189
1111	20	0.4180436378088217
1111	21	0.3108141543988552
1111	22	-0.14876263897578923
1111	23	0.2872334499496034
1111	24	0.30753409448953306
1111	25	0.3297007234374334
1112	1	0.1832366677722277
1112	2	0.16415937946865
1112	3	0.12955684581588045
1112	4	0.2544214772904141
1112	5	0.192137116601657
1112	6	-0.08791954373928235
1112	7	-0.17435618789047425
1112	8	0.056482025065243135
1112	9	-0.10435531005344245
1112	10	0.1470504175404983
1112	11	-0.1117822684811074
1112	12	-0.025632763348646392
1112	13	0.0109027155604835
1112	14	-0.09241779265170891
1112	15	-0.33853786807462427
1112	16	-0.03571109391049987
1112	17	0.20784417470337502
1112	18	-0.016546017825596948
1112	19	0.05466198118322296
1112	20	-0.16215111112123992
1112	21	-0.15474163026735255
1112	22	-0.17344352317716982
1112	23	0.04596929292203811
1112	24	0.08810077044249454
1112	25	0.02957151965301108
1113	1	-0.22475459342493664
1113	2	-0.03514781043155679
1113	3	-0.1886482332711985
1113	4	-0.052223844074955394
1113	5	-0.16947828485420047
1113	6	-0.4918474440549012
1113	7	-0.6224487936591778
1113	8	-0.4145576917902467
1113	9	-0.5446992977935778
1113	10	-0.44160875090494756
1113	11	-0.5382294616652032
1113	12	-0.742380159039449
1113	13	-0.5630049340846739
1113	14	-0.24127071075508155
1113	15	-0.6458171631323637
1113	16	-0.8734124603746594
1113	17	-0.5730777781180336
1113	18	-0.7244264476506359
1113	19	-1.1340633212085436
1113	20	-0.7676876626692319
1113	21	-0.5233717909956468
1113	22	-1.1747023486382844
1113	23	-5.100837577710841
1113	24	-2.539851084044578
1113	25	-1.6107701876062173
1114	1	-0.7741342099450819
1114	2	-1.1197117584960488
1114	3	-0.7561838758249486
1114	4	-0.6635773282100388
1114	5	-0.7603256392383173
1114	6	-1.354435658689475
1114	7	-1.25040803108962
1114	8	-0.8765911008537159
1114	9	-1.2479894429978842
1114	10	-0.8516311955615805
1114	11	-1.1069435858845793
1114	12	-1.951339089159191
1114	13	-1.214767031699722
1114	14	-2.9194189991442236
1114	15	-1.8545360067660965
1114	16	-2.0040894768267563
1114	17	-1.6293197523697884
1114	18	-1.7066737669135321
1114	19	-1.7386696891253024
1114	20	-1.182629822404948
1114	21	-1.0454172181026493
1114	22	-1.165655889845354
1114	23	-5.4112886110430765
1114	24	-2.637171170091573
1114	25	-2.4418782621949155
1115	1	-1.637911334088809
1115	2	-1.5588803457315592
1115	3	-1.079321681985821
1115	4	-1.3627094584741428
1115	5	-1.2147398075413243
1115	6	-1.3114591748977773
1115	7	-1.980248148808716
1115	8	-2.3019188698159176
1115	9	-3.687318416003805
1115	10	-4.3617390181102245
1115	11	-2.980495579838376
1115	12	-3.378995497091804
1115	13	-2.7503852599688727
1115	14	-3.5680520792710624
1115	15	-3.0851122748693847
1115	16	-3.1507552935165144
1115	17	-3.034354322609012
1115	18	-5.822546869150592
1115	19	-3.828630270769377
1115	20	-4.128095141452171
1115	21	-3.6258020248465925
1115	22	-3.21567567752437
1115	23	-3.7430768271147876
1115	24	-3.3332719416215717
1115	25	-3.727535064256948
1116	1	-0.48960991181925095
1116	2	-0.38843486827667917
1116	3	-0.8403151139639071
1116	4	-0.41718181853247316
1116	5	-0.48426511491446433
1116	6	-2.7974475883696037
1116	7	-1.3390286269023157
1116	8	-1.8705118963318803
1116	9	-2.764715395609592
1116	10	-1.942514157508569
1116	11	-1.613018569574077
1116	12	-1.2981419694551803
1116	13	-2.8373725635627842
1116	14	-6.012130988438585
1116	15	-3.738404829728708
1116	16	-2.4833748376536526
1116	17	-3.6300427206520425
1116	18	-2.171479461901715
1116	19	-1.9079417483005727
1116	20	-2.548677487259603
1116	21	-3.4917398882735426
1116	22	-2.7882515730996253
1116	23	-1.8044386423375336
1116	24	-2.73460064299805
1116	25	-2.567153972993129
1201	1	0.5351834514457678
1201	2	0.5810804089111414
1201	3	0.8907583465562574
1201	4	0.846896427099189
1201	5	0.253399482720539
1201	6	0.8724634539260308
1201	7	1.0041029187511228
1201	8	0.9552996923769044
1201	9	1.4056048094970635
1201	10	1.2465594065393049
1201	11	1.4095451418333056
1201	12	1.2455193183381397
1201	13	1.377295942243137
1201	14	1.420188153986615
1201	15	1.4852663997693796
1201	16	1.330167956589598
1201	17	1.5981079852394444
1201	18	1.3987157487238804
1201	19	1.5829185060426454
1201	20	1.5371721022073643
1201	21	1.6824816366591264
1201	22	1.4277928616010342
1201	23	1.8001376629108634
1201	24	1.514300136352432
1201	25	1.7556918514147455
1202	1	0.9730493887471177
1202	2	0.9901523082721084
1202	3	0.9001723450172285
1202	4	1.2449776609979466
1202	5	1.0505238428530248
1202	6	1.3070422287261252
1202	7	1.3169479453495327
1202	8	1.2754885578458044
1202	9	1.4218817006610394
1202	10	1.4133348088612294
1202	11	1.403113009610749
1202	12	1.6146183179701126
1202	13	1.5371540840344338
1202	14	1.9573255623977346
1202	15	1.6780170772761522
1202	16	1.3903280489312593
1202	17	1.7212009291318466
1202	18	1.8359836399213165
1202	19	1.8381169067920808
1202	20	1.6143869638621595
1202	21	1.931599204995301
1202	22	1.7563627368059613
1202	23	1.838855920463807
1202	24	1.7239090225647544
1202	25	2.123787137305875
1203	1	0.7260981692349233
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2211	13	0.04424594460821396
2211	14	0.34852077779816426
2211	15	-0.0969941366290854
2211	16	-0.14291136794566484
2211	17	0.3385109936118056
2211	18	-0.1254704297917968
2211	19	0.09206093071507837
2211	20	-0.09837399305775563
2211	21	0.10682722106857057
2211	22	0.11910500388380285
2211	23	0.362237847386524
2211	24	0.04992995518787069
2211	25	0.3619190875908913
2212	1	0.33383671899611045
2212	2	0.2840461380943857
2212	3	-0.09266517313695743
2212	4	0.33018068844258863
2212	5	0.03284409768541252
2212	6	-0.12302391240489641
2212	7	0.051578648010149664
2212	8	0.15933638458373878
2212	9	0.17351277014852684
2212	10	-0.20385514756872425
2212	11	-0.14129362920701993
2212	12	0.3014239671039505
2212	13	0.30476270970632413
2212	14	0.3239194251595663
2212	15	-0.16958320121281645
2212	16	-0.19146966476410654
2212	17	0.175615203937582
2212	18	0.3390904389451066
2212	19	0.0632849733898766
2212	20	0.34419744912170813
2212	21	0.36652954532080884
2212	22	0.3840278463299853
2212	23	0.5783190057023972
2212	24	0.43761094204251094
2212	25	0.5134003221117567
2213	1	-0.1540431638239781
2213	2	-0.1077945889183809
2213	3	-0.09643583308986692
2213	4	-0.04138472794239689
2213	5	-0.08845080638713654
2213	6	0.07930132789939393
2213	7	-0.16503809286527726
2213	8	0.07893012074244865
2213	9	-0.4966802697687953
2213	10	-0.05640893920916312
2213	11	-0.31057605765967367
2213	12	-0.21199140382330484
2213	13	-0.3118047853010921
2213	14	-0.3773713605208613
2213	15	-0.3804157380646558
2213	16	-0.22967212258944159
2213	17	-0.06530296438810268
2213	18	-0.0012946695706403943
2213	19	-0.10508830057644758
2213	20	0.14938147519967515
2213	21	-0.16413936314987154
2213	22	-0.47441408045588673
2213	23	-0.25351655389384575
2213	24	0.018332884524888016
2213	25	0.24224791052120054
2214	1	-2.5017326242638234
2214	2	-1.2123401334733508
2214	3	-1.4205756535891005
2214	4	-3.1819977017530867
2214	5	-1.5217961773090138
2214	6	-1.1546783874611393
2214	7	-1.078515319675855
2214	8	-0.9295430909330769
2214	9	-0.6987313237352311
2214	10	-0.7184446517640524
2214	11	-0.7047325640283901
2214	12	-0.6919154680050212
2214	13	-0.9613332609723955
2214	14	-0.77081499151614
2214	15	-1.1770056351406737
2214	16	-1.0118989878815299
2214	17	-3.1669528435692733
2214	18	-3.0572948602960714
2214	19	-1.7074360732838443
2214	20	-1.706370824579011
2214	21	-1.6776592426280743
2214	22	-2.8684341868765024
2214	23	-1.6295204027955315
2214	24	-2.754576183739701
2214	25	-2.7485273101809504
2215	1	-1.9211290301735033
2215	2	-3.601535587617345
2215	3	-2.129605956888728
2215	4	-3.8095011971339616
2215	5	-2.1114551270116344
2215	6	-2.168072348131787
2215	7	-2.2643107139387055
2215	8	-1.6385386765044991
2215	9	-4.417153050256186
2215	10	-2.4531283010472293
2215	11	-1.9924940649007752
2215	12	-1.870463328198671
2215	13	-2.0785126760814414
2215	14	-1.662063929848017
2215	15	-1.9900376148899568
2215	16	-1.95334615055436
2215	17	-2.4445774621603107
2215	18	-2.0544959170481683
2215	19	-2.0265447664363414
2215	20	-1.8360651474805465
2215	21	-2.32470201431374
2215	22	-2.245377754736886
2215	23	-2.341765151114224
2215	24	-2.180954602533266
2215	25	-3.3333582737284466
2216	1	-3.542109551019049
2216	2	-3.3297130305941423
2216	3	-4.875110633981002
2216	4	-5.538888585808898
2216	5	-5.276330694780736
2216	6	-4.0883930184306045
2216	7	-5.951942141168175
2216	8	-3.474659891695147
2216	9	-4.232400794269417
2216	10	-3.3911641998030824
2216	11	-3.5265482432720248
2216	12	-4.129089741507521
2216	13	-3.5927980484345987
2216	14	-4.539313239020359
2216	15	-3.8981337750468086
2216	16	-4.381891884628555
2216	17	-4.037755422000384
2216	18	-3.5028239857764163
2216	19	-4.338722550751033
2216	20	-4.855706645565377
2216	21	-4.451551881837176
2216	22	-4.163977860052114
2216	23	-4.181515683067126
2216	24	-4.800817623359734
2216	25	-4.669934779552349
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	102437.0
3	2.0
4	892.0
5	5328.0
6	6548.0
7	5791.0
8	5257.0
9	4787.0
10	4455.0
11	4251.0
12	4037.0
13	3840.0
14	3834.0
15	4027.0
16	4072.0
17	4473.0
18	5070.0
19	5924.0
20	7857.0
21	10130.0
22	12768.0
23	14955.0
24	17784.0
25	20121.0
26	22040.0
27	24420.0
28	27547.0
29	32287.0
30	38775.0
31	47443.0
32	59288.0
33	75143.0
34	95317.0
35	123415.0
36	165721.0
37	257014.0
38	616480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03585531597582	18.115221432089896	12.305925448157431	24.54299780377685
2	15.414402435577573	19.630497016016026	41.137857835310996	23.817242713095407
3	19.79693614001815	25.166421552804902	28.329231224920132	26.70741108225681
4	12.72226697805879	15.955211613732716	37.05647644079888	34.26604496740961
5	13.571517309360503	38.572154749963246	34.15026997643911	13.706057964237143
6	32.71929276945251	35.368910192903265	18.150875488229563	13.760921549414668
7	29.395689850318952	31.279120707316636	20.999697630497955	18.325491811866456
8	26.471998405788682	35.767184332855685	20.055352146402463	17.70546511495317
9	28.334896820691885	14.347928053523384	19.25157814505254	38.06559698073219
10	17.041431426382708	27.876645505673185	32.60439836669633	22.47752470124778
11	35.21343226133583	21.884590391055873	24.027897740135383	18.87407960747291
12	24.996565861349843	24.107436145381612	30.213524696750625	20.68247329651792
13	29.908629320373908	21.502211929381122	25.14069868630558	23.44846006393939
14	22.116779959793202	21.89267292696923	26.618030518296376	29.372516594941196
15	24.130025493917508	30.446013263786043	22.623408801699014	22.800552440597436
16	22.895477028464214	27.165625134025735	27.13310607997733	22.80579175753272
17	22.409578632681892	27.993675635232705	26.810191957067293	22.786553775018106
18	22.35380801228416	26.269599863605908	29.302739501401405	22.073852622708532
19	24.371500797025597	26.03901081956817	26.974864375344765	22.61462400806147
20	25.197719929658557	25.949454712675628	27.19036545950779	21.662459898158016
21	24.248797147136468	25.780653272742377	26.23133969824313	23.73920988187803
22	24.74803471968082	27.239996047177588	26.01847618695981	21.99349304618178
23	23.292828394322395	26.918781453041618	27.082802838259497	22.705587314376494
24	24.2101885688195	25.83234840468705	27.539962224344393	22.417500802149053
25	23.55495401293534	26.492034717912887	27.336637474476515	22.616373794675262
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	272.0
1	272.0
2	563.0
3	854.0
4	854.0
5	854.0
6	1488.5
7	2123.0
8	2123.0
9	2123.0
10	2447.5
11	2772.0
12	2772.0
13	2772.0
14	3743.0
15	4714.0
16	4714.0
17	4714.0
18	9895.5
19	15077.0
20	15077.0
21	15077.0
22	25200.5
23	35324.0
24	35324.0
25	35324.0
26	54377.0
27	73430.0
28	73430.0
29	73430.0
30	96789.5
31	120149.0
32	120149.0
33	120149.0
34	137738.0
35	155327.0
36	155327.0
37	155327.0
38	168949.5
39	182572.0
40	182572.0
41	182572.0
42	197438.0
43	212304.0
44	212304.0
45	212304.0
46	232441.0
47	252578.0
48	252578.0
49	252578.0
50	247227.5
51	241877.0
52	241877.0
53	241877.0
54	217590.0
55	193303.0
56	193303.0
57	193303.0
58	169637.0
59	145971.0
60	145971.0
61	145971.0
62	123401.0
63	100831.0
64	100831.0
65	100831.0
66	78805.5
67	56780.0
68	56780.0
69	56780.0
70	42541.0
71	28302.0
72	28302.0
73	28302.0
74	20211.0
75	12120.0
76	12120.0
77	12120.0
78	8367.0
79	4614.0
80	4614.0
81	4614.0
82	3096.0
83	1578.0
84	1578.0
85	1578.0
86	1038.0
87	498.0
88	498.0
89	498.0
90	315.5
91	133.0
92	133.0
93	133.0
94	77.0
95	21.0
96	21.0
97	21.0
98	13.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3409220354428732
2	0.010252070755561341
3	0.009167195543332628
4	0.010577533319229955
5	0.011987871095127283
6	0.07675492126518148
7	0.0766464337439586
8	0.1012731010615504
9	0.08104017835348488
10	0.08782064842991436
11	0.08228778484754791
12	0.09378746209717227
13	0.07990105938064475
14	0.0842405602295596
15	0.08255900365060509
16	0.08315568501733088
17	0.09568599371857252
18	0.09807271918547569
19	0.09123800534843479
20	0.0898819113331489
21	0.07870769664719317
22	0.09807271918547569
23	0.09205166175760633
24	0.08695274826013137
25	0.08673577321768564
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1843530.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.66732331586949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.07140333805206	40.28881274719284
2	13.588951251955054	14.585652807245229
3	4.859637919498861	7.824112782714142
4	2.3040618842936076	4.946113363366261
5	1.216505481198239	3.2643296487496634
6	0.7155079603538389	2.303963822603268
7	0.4637011350770789	1.7419919116682085
8	0.3250894591390975	1.395734488815927
9	0.24339977574306076	1.1756353013831673
>10	1.0912498609255197	10.16599015854502
>50	0.0690276864085488	2.5819025386542824
>100	0.04251491806903798	4.318989190124381
>500	0.006305209269691379	2.363009817403135
>1k	0.002542423092617491	2.748166882291668
>5k	1.0169692370469967E-4	0.29559453924277945
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5416	0.2937842074715356	No Hit
GTCCTACAGTGGACATTTCTAAATT	4522	0.2452902854849121	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4193	0.22744408824374976	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3896	0.21133369134215338	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3295	0.1787331912146805	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3125	0.16951175191073647	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3041	0.16495527601937587	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2594	0.14070831502606412	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2168	0.11760047300559252	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2166	0.11749198548436966	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.424376061143567E-5
2	0.0	0.0	0.0	0.0	5.424376061143567E-5
3	0.0	0.0	0.0	0.0	5.424376061143567E-5
4	0.0	0.0	0.0	0.0	5.424376061143567E-5
5	0.0	0.0	0.0	0.0	5.424376061143567E-5
6	0.0	0.0	0.0	0.0	5.424376061143567E-5
7	0.0	0.0	0.0	0.0	5.424376061143567E-5
8	0.0	0.0	0.0	0.0	5.424376061143567E-5
9	0.0	0.0	0.0	0.0	5.424376061143567E-5
10	0.0	0.0	0.0	0.0	5.424376061143567E-5
11	0.0	0.0	0.0	0.0	5.424376061143567E-5
12	0.0	0.0	0.0	0.0	1.0848752122287133E-4
13	0.0	0.0	0.0	0.0	1.0848752122287133E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTTCG	45	6.769987E-4	14.775814	14
TAGGACC	630	0.0	12.664639	4
GGCGAGG	990	0.0	11.705198	19
TAGAAAT	515	0.0	11.619499	4
ATTTAGA	525	0.0	11.429513	1
GCGTGCG	160	4.5474735E-11	11.279445	9
GTCGAGG	95	1.3628778E-5	10.9982395	19
AAATGTC	510	0.0	10.988736	7
TGTAGGA	2085	0.0	10.933502	2
GAACCGC	70	0.0014932838	10.8557005	6
TGTCGAG	105	3.4722689E-6	10.8557005	18
CAGTCCG	70	0.001493622	10.855405	9
AATGTCC	520	0.0	10.777414	8
GTATCAA	3810	0.0	10.774542	1
GTCCTAC	2035	0.0	10.718094	1
GCTCTAT	80	3.682339E-4	10.715169	1
CGTGCGC	160	5.456968E-10	10.68579	10
GACAGTC	125	2.244451E-7	10.638586	7
TAATACC	90	9.531801E-5	10.553866	4
AGGACCT	1335	0.0	10.53015	5
>>END_MODULE