FastQCFastQC Report
Thu 26 May 2016
SRR1512886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512886_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843530
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68910.3737937543734032No Hit
TATCAACGCAGAGTACTTTTTTTTT48630.26378740785341165No Hit
GTCCTACAGTGGACATTTCTAAATT42450.23026476379554442No Hit
CTGTAGGACGTGGAATATGGCAAGA39620.2149137795425081No Hit
GGTATCAACGCAGAGTACTTTTTTT39540.21447982945761662No Hit
GTCCTAAAGTGTGTATTTCTCATTT37670.20433624622327815No Hit
CTTTAGGACGTGAAATATGGCGAGG35300.19148047495836792No Hit
GTACTGGTTCACTATCGGTCAGTCA24870.1349042326406405No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23150.12557430581547357No Hit
GTACATGGGAAGCAGTGGTATCAAC20900.11336945967790055No Hit
GTCCTACAGTGTGCATTTCTCATTT20490.11114546549283169No Hit
CCCATGTACTCTGCGTTGATACCAC19620.10642625831963679No Hit
CTGAAGGACCTGGAATATGGCGAGA18680.10132734482216184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGC402.7440893E-416.6387634
TAGGACC5850.013.9773734
ATCCCGT1052.001434E-812.66339210
TCTACGA550.003047374512.1022342
GGACCGT802.84365E-511.8845076
TGGACCG1052.6916678E-711.7716425
TAGAAAT5750.011.5747924
GACGTGG9900.011.4249217
TAGGACA5050.011.2964724
CTAGGAC2550.011.1869383
GGTATCA17900.011.15932751
TGTAGGA21300.011.026752
GTAGGAC20350.010.9340643
GGCGAGG9900.010.93122719
ATTTAGA5400.010.9211851
CTGTAGG21100.010.9094961
GGCCGTT803.753951E-410.6928627
GGGTTAT909.395334E-510.5688891
TCCCGTT1355.7321813E-810.54996511
GGACGTG21750.010.4911526
Produced by FastQC (version 0.11.5)