##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512886_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1843530 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.496500735002957 31.0 31.0 34.0 27.0 34.0 2 30.4777237148297 31.0 30.0 34.0 27.0 34.0 3 30.66771845318492 31.0 31.0 34.0 27.0 34.0 4 34.29616116906153 37.0 35.0 37.0 30.0 37.0 5 33.93349118267671 37.0 35.0 37.0 30.0 37.0 6 33.825948045326086 37.0 35.0 37.0 29.0 37.0 7 33.66779330957456 37.0 35.0 37.0 28.0 37.0 8 33.625234739874045 37.0 35.0 37.0 28.0 37.0 9 34.97611484488996 39.0 35.0 39.0 28.0 39.0 10 34.8891941004486 39.0 35.0 39.0 27.0 39.0 11 34.898493108330214 39.0 35.0 39.0 27.0 39.0 12 34.781334179535996 39.0 35.0 39.0 27.0 39.0 13 34.773460155245644 39.0 35.0 39.0 27.0 39.0 14 35.864219730625486 40.0 36.0 41.0 27.0 41.0 15 35.86742065493917 40.0 36.0 41.0 27.0 41.0 16 35.867095192375494 40.0 36.0 41.0 27.0 41.0 17 35.853170819026545 40.0 36.0 41.0 27.0 41.0 18 35.77532234354744 40.0 36.0 41.0 27.0 41.0 19 35.77904129577495 40.0 36.0 41.0 27.0 41.0 20 35.76626580527575 40.0 36.0 41.0 27.0 41.0 21 35.72841450912109 40.0 36.0 41.0 27.0 41.0 22 35.69743806718632 40.0 36.0 41.0 27.0 41.0 23 35.550287220712434 40.0 36.0 41.0 27.0 41.0 24 35.51503637044149 40.0 36.0 41.0 27.0 41.0 25 35.44603017037965 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5815692639975154 1101 2 0.4836250910270614 1101 3 0.3920704995636477 1101 4 0.3968104829641703 1101 5 0.5154627639889426 1101 6 0.5806247466945536 1101 7 0.6519766307665051 1101 8 0.4104173054741054 1101 9 0.7985688180173938 1101 10 0.3712046800199076 1101 11 0.7800221637779998 1101 12 0.779762467057104 1101 13 0.8384744369169397 1101 14 0.9181887604432575 1101 15 0.6124022672146836 1101 16 0.832981251966892 1101 17 0.9637961389563472 1101 18 0.6831881371242474 1101 19 0.6511652935487788 1101 20 0.7557047667644738 1101 21 0.8718273130846868 1101 22 0.7782668703796105 1101 23 0.9961333314756189 1101 24 0.9316468399490248 1101 25 1.03086371934549 1102 1 0.7737541806604575 1102 2 0.8327007218267681 1102 3 0.4790437780836889 1102 4 0.5670857922198778 1102 5 0.8740670684335825 1102 6 0.8272071779545129 1102 7 0.874133028319271 1102 8 0.8860451494722028 1102 9 1.045981408131638 1102 10 0.8940204336281994 1102 11 1.067680585177527 1102 12 1.0830916205045327 1102 13 1.1215264890640455 1102 14 1.1753937107687094 1102 15 1.2553223643667764 1102 16 1.2206660187497391 1102 17 1.1664234392899928 1102 18 0.5464031163830754 1102 19 0.9960298522124091 1102 20 1.272077072646475 1102 21 1.1588514205068208 1102 22 1.0252115506602308 1102 23 1.1962656169515853 1102 24 1.2552625203860401 1102 25 1.2370911675111813 1103 1 0.7968053408465465 1103 2 1.03161681653701 1103 3 0.6955233025222611 1103 4 0.4939526974809141 1103 5 0.7975722339898397 1103 6 0.7604439786839379 1103 7 0.8638669788390558 1103 8 0.9200219636988436 1103 9 1.0109824311917777 1103 10 0.9395466096089038 1103 11 1.0604110249856973 1103 12 1.2581919521914031 1103 13 1.222322142673832 1103 14 1.1947546751015992 1103 15 1.3367548100907385 1103 16 1.3358675256993706 1103 17 1.3255833274354174 1103 18 1.4304567498619107 1103 19 1.3572373862426232 1103 20 1.1962306249984351 1103 21 1.3362644065501286 1103 22 1.1710747980325849 1103 23 1.4272779798256323 1103 24 1.4189516931791033 1103 25 1.375309732642421 1104 1 0.7544540239885471 1104 2 0.8569819352760071 1104 3 0.7499255449385558 1104 4 0.61185833366212 1104 5 0.6600028988308253 1104 6 0.6940934725915682 1104 7 0.6946018843616031 1104 8 0.6372236859615938 1104 9 0.8129056523882099 1104 10 0.8362408970016304 1104 11 0.8966971304896418 1104 12 0.9251772070495221 1104 13 0.782547266142295 1104 14 1.0990382640732577 1104 15 0.99111528579256 1104 16 1.0848529718420252 1104 17 0.9468741733737147 1104 18 0.49697824940979984 1104 19 0.9679753365235584 1104 20 0.868044936854524 1104 21 0.8067845425376348 1104 22 0.8334707596475681 1104 23 0.8983376391022517 1104 24 1.0863683253692145 1104 25 0.9958891843840973 1105 1 0.6641898099207388 1105 2 0.6644604691282936 1105 3 0.7541100691012552 1105 4 0.5481193331329592 1105 5 0.5438836467722723 1105 6 0.7133657530939459 1105 7 0.7184902081048321 1105 8 0.6732524467081547 1105 9 0.9844952508850255 1105 10 0.5896550440243615 1105 11 0.8859804862814187 1105 12 0.9554217407650896 1105 13 0.9522684530795473 1105 14 1.0349499752698677 1105 15 1.0591528548122184 1105 16 1.0446578503633503 1105 17 0.7956212324115839 1105 18 0.7037367628574032 1105 19 1.0992337529344027 1105 20 1.0631838083903062 1105 21 0.9321640619156497 1105 22 0.7977631063374844 1105 23 1.0142647991221878 1105 24 0.9666618508107305 1105 25 0.8228172297887113 1106 1 0.036796836300347024 1106 2 0.26822848802691723 1106 3 -1.6126565246408333 1106 4 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0.7659414475170152 1107 17 0.8297558808153696 1107 18 0.8854741847340364 1107 19 0.8732007925626988 1107 20 0.9054320891536634 1107 21 0.7639483652776775 1107 22 0.8427581345111648 1107 23 1.0330420284562578 1107 24 1.002070809653567 1107 25 0.9943161234453726 1108 1 0.3485641675842892 1108 2 0.28434443709319623 1108 3 0.25662632139849606 1108 4 0.054027501111669096 1108 5 0.31588577961370845 1108 6 0.23419015850371494 1108 7 0.1933166871947165 1108 8 0.11338915486611967 1108 9 0.35736022882467466 1108 10 0.22800417014340724 1108 11 0.19527283346128144 1108 12 0.2671131553198336 1108 13 0.3482192399630293 1108 14 0.37728771207632406 1108 15 0.3605676446871371 1108 16 0.3558077112963218 1108 17 0.3608522763282451 1108 18 0.49871851765799846 1108 19 0.42337028209145444 1108 20 0.5453485665987472 1108 21 0.5426982658811283 1108 22 0.5680768661228015 1108 23 0.6322434691908825 1108 24 0.5671674525534129 1108 25 0.612997815925695 1109 1 0.2716962940720826 1109 2 0.202560018569109 1109 3 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2213 19 -0.5991313949593646 2213 20 -0.5922559243571328 2213 21 -0.6063713214977042 2213 22 -0.6391084517858019 2213 23 -0.6840946380474975 2213 24 -0.49000354318027917 2213 25 -0.49670337706779577 2214 1 -1.2036745570362726 2214 2 -0.4762756804639352 2214 3 -0.5659265813086769 2214 4 -0.5676046678136331 2214 5 -0.8015559229722484 2214 6 -1.4116910603221413 2214 7 -0.8454854747092284 2214 8 -0.7363724981670217 2214 9 -0.6004558039195231 2214 10 -0.8909265878129133 2214 11 -0.940549994384952 2214 12 -0.5293924214916927 2214 13 -0.8582599555933967 2214 14 -0.5855015282086882 2214 15 -0.7491611047522255 2214 16 -0.8503187813775241 2214 17 -0.9152353524122461 2214 18 -0.7805699624142264 2214 19 -0.8528269606973637 2214 20 -1.086073893089626 2214 21 -1.22398260014495 2214 22 -1.6982226520235315 2214 23 -1.7074854704076614 2214 24 -1.1900190200798306 2214 25 -1.6513058786975634 2215 1 -1.5585640352315941 2215 2 -1.0418073366439948 2215 3 -1.1081001499522927 2215 4 -1.1321388142269058 2215 5 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-2.549392032323638 2216 17 -2.947418249088976 2216 18 -3.3769898063278276 2216 19 -3.476390386505571 2216 20 -2.8229838391697157 2216 21 -3.430259146259772 2216 22 -2.975993104994359 2216 23 -4.589199420370104 2216 24 -3.5536695674658 2216 25 -3.5419502447275164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40221.0 3 0.0 4 570.0 5 5752.0 6 7348.0 7 5701.0 8 4725.0 9 4072.0 10 3416.0 11 3085.0 12 2736.0 13 2713.0 14 2655.0 15 2686.0 16 2783.0 17 2961.0 18 3214.0 19 4050.0 20 5433.0 21 7190.0 22 8992.0 23 11058.0 24 13081.0 25 15472.0 26 17225.0 27 19055.0 28 21959.0 29 26281.0 30 33226.0 31 42296.0 32 53752.0 33 69249.0 34 90264.0 35 117719.0 36 160458.0 37 248267.0 38 783865.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.17957800006081 19.001181391348915 13.279359963863325 26.539880644726953 2 13.966053904810277 20.089276993103987 40.07327963255995 25.871389469525784 3 17.8155880766806 25.230878406751817 29.175424185835553 27.778109330732033 4 12.007828739807232 16.51863014411998 36.45419709850586 35.01934401756693 5 13.187250779872508 38.58239522582395 34.43108639631086 13.799267597992676 6 32.758853703903725 35.83572918458919 17.714626570148734 13.690790541358355 7 28.982710629391278 31.38130411748869 21.087962359848188 18.548022893271845 8 26.434270872395953 36.190379022511465 19.78888140574962 17.586468699342962 9 27.68852659134374 14.308028266442415 19.34601977565885 38.657425366554996 10 16.421940928270043 28.414777429552146 32.64721125537602 22.516070386801797 11 35.02968042350545 21.918760859270364 24.061891941885715 18.989666775338478 12 24.65647389136482 24.24671607916994 30.355329563749507 20.741480465715735 13 29.696532105473295 21.391497561060287 25.423613883729406 23.48835644973701 14 21.823734421827748 21.959398435063264 26.699014116976986 29.517853026132002 15 24.03876565754218 30.53435694286438 22.617387148242727 22.809490251350717 16 22.844180580035164 27.186334167131847 27.065522892570186 22.9039623602628 17 22.215260633736005 28.137257933362953 26.960930299671976 22.68655113322907 18 22.24311324168167 26.268763266716537 29.445126446421316 22.042997045180478 19 24.23376978431315 25.912310564760844 27.24954516014594 22.604374490780067 20 24.956184435995645 25.832766935915636 27.48286839925512 21.728180228833597 21 23.932386127244136 25.58893902828654 26.70614533988382 23.772529504585506 22 24.425163748796464 26.963778630612552 26.458659361820423 22.15239825877056 23 22.905784012193998 26.846918683178465 27.39250242740829 22.85479487721925 24 24.0906847189902 25.794969433640897 27.68498478462515 22.42936106274376 25 23.20016490103226 26.420616968533196 27.67711943933649 22.70209869109806 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 86.0 1 86.0 2 290.0 3 494.0 4 494.0 5 494.0 6 1144.0 7 1794.0 8 1794.0 9 1794.0 10 2106.5 11 2419.0 12 2419.0 13 2419.0 14 3486.0 15 4553.0 16 4553.0 17 4553.0 18 9950.0 19 15347.0 20 15347.0 21 15347.0 22 25953.5 23 36560.0 24 36560.0 25 36560.0 26 57037.0 27 77514.0 28 77514.0 29 77514.0 30 100419.0 31 123324.0 32 123324.0 33 123324.0 34 139921.5 35 156519.0 36 156519.0 37 156519.0 38 170082.0 39 183645.0 40 183645.0 41 183645.0 42 199290.0 43 214935.0 44 214935.0 45 214935.0 46 233938.5 47 252942.0 48 252942.0 49 252942.0 50 247772.0 51 242602.0 52 242602.0 53 242602.0 54 216849.0 55 191096.0 56 191096.0 57 191096.0 58 166866.5 59 142637.0 60 142637.0 61 142637.0 62 120756.5 63 98876.0 64 98876.0 65 98876.0 66 76453.5 67 54031.0 68 54031.0 69 54031.0 70 40427.0 71 26823.0 72 26823.0 73 26823.0 74 19038.5 75 11254.0 76 11254.0 77 11254.0 78 7731.5 79 4209.0 80 4209.0 81 4209.0 82 2801.0 83 1393.0 84 1393.0 85 1393.0 86 890.5 87 388.0 88 388.0 89 388.0 90 237.0 91 86.0 92 86.0 93 86.0 94 44.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08863430483908588 2 0.03938097020390229 3 0.019093803735225356 4 0.003580088200354754 5 0.015188252971201988 6 0.060156330518082154 7 0.06737075067940311 8 0.0471378279713376 9 0.019256535017059664 10 0.0463784153227775 11 0.0032003818760747045 12 1.0848752122287133E-4 13 0.0057498386248121805 14 2.712188030571783E-4 15 0.013506696392247481 16 0.008624757937218272 17 0.0043937446095262895 18 0.005478619821755002 19 0.001301850254674456 20 3.797063242800497E-4 21 1.62731281834307E-4 22 2.712188030571783E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1843530.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.91267718745544 #Duplication Level Percentage of deduplicated Percentage of total 1 72.62866838800471 36.97719948193234 2 14.753771311153216 15.023079921245696 3 5.400499442076588 8.248616542364354 4 2.513233038828825 5.118216896109586 5 1.3540885432379774 3.447013644255348 6 0.8055911052958606 2.4608879933408145 7 0.5206979921545729 1.8557090150705384 8 0.36960448959332515 1.505404325255936 9 0.27060480359041844 1.2399493509516384 >10 1.2482662691389304 10.951065554165625 >50 0.07554381984368747 2.6461971341878523 >100 0.05023932291504873 4.82453638238693 >500 0.006198901182359026 2.2671153465838567 >1k 0.002885695377994719 3.0600398096795733 >5k 1.06877606592397E-4 0.3749686024699826 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6891 0.3737937543734032 No Hit TATCAACGCAGAGTACTTTTTTTTT 4863 0.26378740785341165 No Hit GTCCTACAGTGGACATTTCTAAATT 4245 0.23026476379554442 No Hit CTGTAGGACGTGGAATATGGCAAGA 3962 0.2149137795425081 No Hit GGTATCAACGCAGAGTACTTTTTTT 3954 0.21447982945761662 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3767 0.20433624622327815 No Hit CTTTAGGACGTGAAATATGGCGAGG 3530 0.19148047495836792 No Hit GTACTGGTTCACTATCGGTCAGTCA 2487 0.1349042326406405 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2315 0.12557430581547357 No Hit GTACATGGGAAGCAGTGGTATCAAC 2090 0.11336945967790055 No Hit GTCCTACAGTGTGCATTTCTCATTT 2049 0.11114546549283169 No Hit CCCATGTACTCTGCGTTGATACCAC 1962 0.10642625831963679 No Hit CTGAAGGACCTGGAATATGGCGAGA 1868 0.10132734482216184 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.424376061143567E-5 0.0 9 0.0 0.0 0.0 5.424376061143567E-5 0.0 10 0.0 0.0 0.0 5.424376061143567E-5 0.0 11 0.0 0.0 0.0 5.424376061143567E-5 0.0 12 0.0 0.0 0.0 5.424376061143567E-5 0.0 13 0.0 0.0 0.0 5.424376061143567E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATCGC 40 2.7440893E-4 16.638763 4 TAGGACC 585 0.0 13.977373 4 ATCCCGT 105 2.001434E-8 12.663392 10 TCTACGA 55 0.0030473745 12.102234 2 GGACCGT 80 2.84365E-5 11.884507 6 TGGACCG 105 2.6916678E-7 11.771642 5 TAGAAAT 575 0.0 11.574792 4 GACGTGG 990 0.0 11.424921 7 TAGGACA 505 0.0 11.296472 4 CTAGGAC 255 0.0 11.186938 3 GGTATCA 1790 0.0 11.1593275 1 TGTAGGA 2130 0.0 11.02675 2 GTAGGAC 2035 0.0 10.934064 3 GGCGAGG 990 0.0 10.931227 19 ATTTAGA 540 0.0 10.921185 1 CTGTAGG 2110 0.0 10.909496 1 GGCCGTT 80 3.753951E-4 10.692862 7 GGGTTAT 90 9.395334E-5 10.568889 1 TCCCGTT 135 5.7321813E-8 10.549965 11 GGACGTG 2175 0.0 10.491152 6 >>END_MODULE