FastQCFastQC Report
Thu 26 May 2016
SRR1512885_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512885_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403493
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65020.46327270602703396No Hit
GTACTGGTTCACTATCGGTCAGTCA39350.2803719006792339No Hit
TATCAACGCAGAGTACTTTTTTTTT36860.2626304513096966No Hit
GGTATCAACGCAGAGTACTTTTTTT36700.2614904384988026No Hit
GGATACCACGTGTCCCGCCCTACTC23660.1685793944109447No Hit
GTCCTACAGTGGACATTTCTAAATT19430.13844030572293556No Hit
GTACATGGGAAGCAGTGGTATCAAC19130.13630278170250937No Hit
ATCATTAACTGAATCCATAGGTTAA18830.1341652576820832No Hit
GTCCTAAAGTGTGTATTTCTCATTT16890.1203426023499939No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16780.1195588435425043No Hit
GTATTTAGCCTTGGAGGATGGTCCC16250.11578255110641807No Hit
CTGTAGGACGTGGAATATGGCAAGA15850.11293251907918316No Hit
GTTCACTATCGGTCAGTCAGGAGTA15590.11107999826148046No Hit
CCCATGTACTCTGCGTTGATACCAC14570.10381241659203144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG1301.891749E-1012.4560741
TAGGACC3900.011.9343064
GTCTTAT802.7891268E-511.9065421
ACCGTGT658.031539E-411.6907498
GTATAAT2550.011.5796961
ATAATGG2500.011.398483
ATTTAGA2800.011.2261691
GGACCGT1104.9748087E-711.2257766
TAAGACC1104.9748087E-711.2257764
GTGTTAC855.1798714E-511.2061571
GTAAGAC855.3289943E-511.1749813
GTTAGAC600.00588100311.0818563
GTCCTAC9450.010.9867771
GTATAGA1657.6397555E-1110.9684511
GTGCAAG1053.3564102E-610.8859811
AAGACCG700.001492903510.8556965
GTATAAA1855.456968E-1210.81242751
GGTATCA18600.010.7542951
CTAGGAC1701.382432E-1010.6162323
TCCTACA10250.010.5644452
Produced by FastQC (version 0.11.5)