##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512885_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1403493
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.69636043785042	31.0	30.0	33.0	22.0	34.0
2	28.81773047674623	31.0	30.0	34.0	18.0	34.0
3	28.917646899557035	31.0	30.0	34.0	22.0	34.0
4	32.35926007468509	35.0	33.0	37.0	22.0	37.0
5	32.53787514437194	35.0	33.0	37.0	25.0	37.0
6	32.48771173066057	36.0	33.0	37.0	23.0	37.0
7	32.474243191807865	36.0	33.0	37.0	23.0	37.0
8	32.380074571087995	36.0	33.0	37.0	22.0	37.0
9	33.655247300841545	38.0	34.0	39.0	20.0	39.0
10	33.51508486326615	38.0	34.0	39.0	18.0	39.0
11	33.58486148488094	38.0	34.0	39.0	19.0	39.0
12	33.469510001118636	38.0	34.0	39.0	18.0	39.0
13	33.43056930102252	38.0	34.0	39.0	17.0	39.0
14	34.26177116665349	39.0	33.0	41.0	17.0	41.0
15	34.42654291827604	39.0	34.0	41.0	17.0	41.0
16	34.371951979810376	39.0	34.0	41.0	17.0	41.0
17	34.13122046208994	39.0	33.0	41.0	17.0	41.0
18	34.14207124652563	39.0	33.0	41.0	17.0	41.0
19	34.25722180303001	39.0	34.0	41.0	18.0	41.0
20	34.226483495108276	39.0	34.0	41.0	16.0	41.0
21	34.121424902012336	39.0	34.0	41.0	11.0	41.0
22	34.133154921328426	39.0	34.0	41.0	10.0	41.0
23	33.79709125731301	39.0	34.0	41.0	8.0	41.0
24	33.861382992291375	39.0	34.0	41.0	2.0	41.0
25	33.58403996314909	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.82213138467921
1101	2	0.6203654124670273
1101	3	0.8454517539237649
1101	4	1.1353146245887444
1101	5	0.8498595732536813
1101	6	0.9045754461790949
1101	7	0.6929631041817075
1101	8	1.009109498067204
1101	9	1.188306903052343
1101	10	1.0922989897564506
1101	11	1.1840880015294317
1101	12	0.9944728189785579
1101	13	1.473289155916099
1101	14	1.6482147498925954
1101	15	1.44934780137271
1101	16	1.3101132305695273
1101	17	0.6046192493870208
1101	18	1.0807551983350834
1101	19	0.5134575459880679
1101	20	0.005052449944976445
1101	21	0.9992259666692078
1101	22	1.1191704910924614
1101	23	0.6407736255919829
1101	24	1.218915568965059
1101	25	0.9395371076826109
1102	1	1.1572604155714714
1102	2	1.1746032851685406
1102	3	1.2230688899460347
1102	4	1.537842185227447
1102	5	1.2831380411132756
1102	6	1.185217857366716
1102	7	1.216138033306457
1102	8	1.3201482196840217
1102	9	1.5232674563644721
1102	10	1.5461592874413412
1102	11	1.3660351877774275
1102	12	1.4918190765710762
1102	13	1.386988279656265
1102	14	1.5534123714478483
1102	15	1.6544002323013132
1102	16	1.6422142959476531
1102	17	1.8698080324849258
1102	18	1.8108913829088849
1102	19	1.5571069115067928
1102	20	1.7805088830540825
1102	21	2.0305345699636064
1102	22	1.8008792947957915
1102	23	1.6864899287528345
1102	24	1.1152914927249853
1102	25	1.5226501411434938
1103	1	0.6369780335694557
1103	2	0.7728479974847922
1103	3	0.8371423455775648
1103	4	0.9796518797547122
1103	5	0.8392061557878634
1103	6	0.47691052135011347
1103	7	0.4256798156824928
1103	8	0.8006442655347641
1103	9	0.7993220950529079
1103	10	0.9099165134168388
1103	11	0.6429059717371644
1103	12	0.9002722958912628
1103	13	1.048056786421533
1103	14	0.9253463928150865
1103	15	1.0199653372537583
1103	16	1.0657668420651873
1103	17	0.5417643007363182
1103	18	1.055939746441723
1103	19	0.8565265320653026
1103	20	1.1349409567534963
1103	21	0.8981357762391013
1103	22	0.9512008943893804
1103	23	0.26473993429324594
1103	24	0.40433498751443864
1103	25	0.8969678181255532
1104	1	1.1428077562263539
1104	2	1.0734090950619333
1104	3	1.0790096158445301
1104	4	1.3222392988805254
1104	5	0.9008551723515978
1104	6	0.8435093412060439
1104	7	1.1161321151961943
1104	8	0.8575151678836477
1104	9	1.3203356668124684
1104	10	1.4401369761656468
1104	11	0.9527910910924149
1104	12	1.3040928145340658
1104	13	1.320548826151473
1104	14	1.5712637169724886
1104	15	1.2109822277190077
1104	16	1.2594740418070884
1104	17	1.4724705480199631
1104	18	1.483794680987188
1104	19	1.1215014465546886
1104	20	1.1221385615316066
1104	21	1.2321662775075026
1104	22	1.0133521348969055
1104	23	1.5161965173004504
1104	24	1.3171140593248651
1104	25	0.9693932782491999
1105	1	0.6548674545819289
1105	2	0.7343963268422584
1105	3	0.7375324256564753
1105	4	0.8369725653832418
1105	5	0.728061690294382
1105	6	0.8169354131832378
1105	7	0.8535891655344869
1105	8	0.9345300028384642
1105	9	0.4118209446729608
1105	10	0.7345873904914413
1105	11	0.8129355564972656
1105	12	0.9479731252243155
1105	13	0.897962481233364
1105	14	1.0008512044675015
1105	15	0.9866316369068073
1105	16	0.8535256360058057
1105	17	1.1704704717794812
1105	18	1.1533536092109031
1105	19	0.864297803084014
1105	20	1.0694240398839483
1105	21	1.018343054451556
1105	22	0.8780737077585883
1105	23	1.118995779210188
1105	24	1.2043037013346023
1105	25	1.573002749789353
1106	1	0.6262207653651757
1106	2	0.7188123137211875
1106	3	0.5546288193645168
1106	4	0.6474323193842295
1106	5	0.47811602763704997
1106	6	0.6362504148223209
1106	7	0.5284958539290585
1106	8	0.6603052981627116
1106	9	0.685659227043729
1106	10	0.5478937677243607
1106	11	0.1981880538541816
1106	12	0.44133469359713473
1106	13	0.5024515573720549
1106	14	0.9662850652226638
1106	15	0.6055625351963698
1106	16	0.7128295488215102
1106	17	1.013807954637656
1106	18	0.840534107217124
1106	19	0.7814579680671514
1106	20	0.8721601965104142
1106	21	0.8408588470652631
1106	22	0.8113168829145678
1106	23	1.0631684022544903
1106	24	0.7213023826922011
1106	25	1.0032157880110049
1107	1	1.0749805044713376
1107	2	1.0516255512919308
1107	3	1.0323904111214866
1107	4	1.2139089835976122
1107	5	1.0746909077223208
1107	6	1.0413373371378754
1107	7	0.8313316786124574
1107	8	1.0114278015254001
1107	9	1.0802870472604624
1107	10	1.273388780794427
1107	11	1.0798498562550165
1107	12	1.1909700005534631
1107	13	0.9799847401246353
1107	14	1.2657404366410816
1107	15	1.0271933754877125
1107	16	1.1177520166539097
1107	17	1.316077324990168
1107	18	1.2692852175386733
1107	19	1.173735713416491
1107	20	1.2813666021140548
1107	21	1.1535802134586817
1107	22	1.220599424852999
1107	23	1.6142919207683093
1107	24	1.392243174002509
1107	25	1.4919115725959387
1108	1	0.9864499924301846
1108	2	0.9400029186054688
1108	3	0.9196981034291802
1108	4	1.015778674190706
1108	5	0.991062975370987
1108	6	0.9902322011391291
1108	7	0.8899666252261298
1108	8	0.8989542998497697
1108	9	1.0017368563166613
1108	10	1.0894097456451846
1108	11	0.7288579226567009
1108	12	0.9194627135142568
1108	13	1.0223875146550654
1108	14	1.134977635612323
1108	15	1.011470516786133
1108	16	0.8080990277987965
1108	17	1.066497011686451
1108	18	1.078502347926019
1108	19	1.1157918664832849
1108	20	1.1495173697860963
1108	21	1.117249646862163
1108	22	1.1627906024697054
1108	23	1.3367110619108544
1108	24	1.1467880263133168
1108	25	1.3750736410239668
1109	1	0.42314086263169415
1109	2	0.45785537085508565
1109	3	0.6333224420535188
1109	4	0.5342033805586794
1109	5	0.4166417963398743
1109	6	0.5832000423339139
1109	7	0.34469757197835094
1109	8	0.20809582486008793
1109	9	0.37104949468957926
1109	10	0.46777760184991735
1109	11	0.4453036617088202
1109	12	0.294129680379811
1109	13	0.3799806701205668
1109	14	0.5572097081103564
1109	15	0.40484362647129757
1109	16	0.03787547344403208
1109	17	0.4389453211914969
1109	18	0.6172420754955326
1109	19	0.5367949998091106
1109	20	0.44092839834251407
1109	21	0.30755932077112647
1109	22	0.4343533252735696
1109	23	0.5914673379437261
1109	24	0.568581527007531
1109	25	0.275372432723465
1110	1	0.2784642854605863
1110	2	0.34219808597825363
1110	3	0.22281626535478338
1110	4	0.28160032945088176
1110	5	0.10248420851343099
1110	6	0.16316002211154768
1110	7	-0.030946437144869776
1110	8	0.09486445240187891
1110	9	0.22611434906151118
1110	10	0.32179525397114617
1110	11	0.140713106790038
1110	12	0.14278859771122399
1110	13	0.27952161462868474
1110	14	0.2050012633417353
1110	15	0.09545797740658202
1110	16	0.19062478939539318
1110	17	0.41400504278659866
1110	18	-0.30774895179308714
1110	19	0.03899456834712112
1110	20	0.15727397701486012
1110	21	0.3766970528556115
1110	22	0.23110655448485318
1110	23	0.5326202445233292
1110	24	0.4177290229456858
1110	25	0.6274606624557535
1111	1	0.33191334940418216
1111	2	0.365227504893884
1111	3	0.44914254787362395
1111	4	0.3333815110701437
1111	5	0.3090156940471047
1111	6	0.06937640917695376
1111	7	0.03900445028523336
1111	8	0.16914697124457234
1111	9	0.36226995324336997
1111	10	0.34137901278466387
1111	11	0.09821371461887196
1111	12	-0.2967027711193069
1111	13	0.14327167541156882
1111	14	0.11172822662886972
1111	15	0.1456549139492509
1111	16	0.3340694769713721
1111	17	-0.10477249185964865
1111	18	0.17653308078653396
1111	19	0.06005073173151487
1111	20	0.2502017669492176
1111	21	0.29222246010093045
1111	22	0.04681187906545148
1111	23	0.45231389879028683
1111	24	0.4802041019634373
1111	25	0.4355452722296391
1112	1	0.5336285815148543
1112	2	0.4056698060288646
1112	3	0.28757403503812284
1112	4	0.6317979950683892
1112	5	0.684995430030348
1112	6	0.32920182687873734
1112	7	0.11761694967633929
1112	8	0.40090243911928525
1112	9	0.1632897788559191
1112	10	0.3934963102662721
1112	11	0.358167925734854
1112	12	0.2750310688144424
1112	13	0.3422228176980937
1112	14	0.3742571369508525
1112	15	0.2271016478378982
1112	16	0.5880319690557414
1112	17	0.8377164749677704
1112	18	0.3632291692467646
1112	19	0.5350114214874679
1112	20	0.43159413871598673
1112	21	0.5411703874232572
1112	22	0.4462045431016932
1112	23	0.7454747931348749
1112	24	0.7023444887333739
1112	25	0.536733055365687
1113	1	0.09159436256337727
1113	2	0.16392345819357956
1113	3	0.15734280512107546
1113	4	0.31023280225446825
1113	5	0.047900031634917184
1113	6	-0.401520197135504
1113	7	-0.3634207995416219
1113	8	-0.2233865203948895
1113	9	-0.19817449120107256
1113	10	-0.20108383474675406
1113	11	-0.17834708482705253
1113	12	-0.38223834052665495
1113	13	-0.12276730747157671
1113	14	0.059056612895815874
1113	15	-0.1568918358281266
1113	16	-0.33064131752749404
1113	17	-0.054723219078404384
1113	18	-0.23288890397649453
1113	19	-0.7389258191738008
1113	20	-0.47682583768123976
1113	21	-0.10366989173442676
1113	22	-0.6553691835644102
1113	23	-4.467899814477743
1113	24	-1.95458762654156
1113	25	-1.1613903169659707
1114	1	-0.6475423147290087
1114	2	-0.9623687931258651
1114	3	-0.8617700212799875
1114	4	-0.584742267431487
1114	5	-0.7094863482464646
1114	6	-1.3188287508457996
1114	7	-1.1078729603967403
1114	8	-1.0407939832045265
1114	9	-1.252385752252728
1114	10	-0.9440475939057862
1114	11	-1.1605939995634316
1114	12	-1.8539854348905749
1114	13	-1.104969263526435
1114	14	-3.2135794617016167
1114	15	-1.8494032526398598
1114	16	-1.874746820014309
1114	17	-1.516420079885087
1114	18	-1.6562658437778595
1114	19	-1.6664074658797787
1114	20	-1.2156316439395667
1114	21	-1.180973962007542
1114	22	-1.4209597976834232
1114	23	-5.664345012331161
1114	24	-2.8111831065573227
1114	25	-2.7412172529758827
1115	1	-1.6927724489769815
1115	2	-1.6883118622878648
1115	3	-1.4674431769240215
1115	4	-1.3735853763470764
1115	5	-1.4721116054378065
1115	6	-1.6281394112792675
1115	7	-2.0669881913630377
1115	8	-2.2771606078577094
1115	9	-3.632832327845666
1115	10	-4.5886901557215936
1115	11	-3.2089238865584626
1115	12	-3.3736681868661833
1115	13	-2.8595038948606906
1115	14	-3.385125342171719
1115	15	-3.1683848211500845
1115	16	-3.2506869615863927
1115	17	-3.036578231373994
1115	18	-5.798916514504118
1115	19	-3.764947060762971
1115	20	-4.266734215390738
1115	21	-3.8326546554251912
1115	22	-3.299492756696136
1115	23	-3.8225205382958016
1115	24	-3.6052116449092644
1115	25	-3.7887637785429895
1116	1	-1.675188000264626
1116	2	-1.6096098874065277
1116	3	-2.077220318803004
1116	4	-1.781236149171903
1116	5	-1.9730158812305447
1116	6	-4.154182732214554
1116	7	-2.7103338633758085
1116	8	-3.216215447202565
1116	9	-4.313593585777497
1116	10	-3.5495958365604245
1116	11	-3.0933994135249563
1116	12	-2.8940968644506526
1116	13	-4.187627871378609
1116	14	-7.471385673465754
1116	15	-5.147585785288758
1116	16	-4.061478309272964
1116	17	-4.930433331542897
1116	18	-3.7446540301253606
1116	19	-3.4006759976872836
1116	20	-4.481776718035096
1116	21	-4.84822019142554
1116	22	-4.630785013210058
1116	23	-3.843652035768205
1116	24	-4.6619185137046735
1116	25	-4.518581726126854
1201	1	0.4758906405639536
1201	2	0.4900288025419002
1201	3	0.8558903175411032
1201	4	0.7984131412404594
1201	5	0.36171317985408535
1201	6	0.9464339923075258
1201	7	1.0492007195471942
1201	8	1.0657536216906394
1201	9	1.2940396207210654
1201	10	1.352516076856034
1201	11	1.4805332604420727
1201	12	1.2591350228790645
1201	13	1.256034043619195
1201	14	1.4586154934577422
1201	15	1.456068539253387
1201	16	1.4051903204425784
1201	17	1.7001034205491123
1201	18	1.4787780118489877
1201	19	1.6746784689577865
1201	20	1.6405569980927694
1201	21	1.6591356798900634
1201	22	1.4716002002333397
1201	23	1.7873503951406562
1201	24	1.7999445723608432
1201	25	1.8261924741761106
1202	1	0.9583470716789613
1202	2	0.9676957063024858
1202	3	1.0650386899949318
1202	4	1.2630276794712785
1202	5	1.1681280665899436
1202	6	1.2777262462420182
1202	7	1.5064586708255447
1202	8	1.4522938229212272
1202	9	1.789526549480101
1202	10	1.5373899451929418
1202	11	1.5436778163743696
1202	12	1.7218221947169141
1202	13	1.6726941499201402
1202	14	2.166971788071109
1202	15	1.938143088026834
1202	16	1.5341758577135138
1202	17	1.9619080505559978
1202	18	1.9929472508695838
1202	19	2.001845749076196
1202	20	1.7924269325121287
1202	21	1.9824194536886566
1202	22	1.9264927368390232
1202	23	1.82402629235699
1202	24	1.8792412090800141
1202	25	2.157688444730425
1203	1	0.7698535003279403
1203	2	0.9483864952659893
1203	3	0.8793585249748475
1203	4	0.9518733143488305
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2211	18	-0.36442421549278947
2211	19	-0.17627420337308308
2211	20	-0.23623524633525506
2211	21	0.06298827676505425
2211	22	-0.024146740571133307
2211	23	0.08268494780540436
2211	24	-0.31621468970958944
2211	25	0.11522216946585928
2212	1	-0.05258665059582057
2212	2	0.14096824563462462
2212	3	-0.21132041508933952
2212	4	0.04712225181088314
2212	5	-0.13565097261955827
2212	6	-0.17171618341564
2212	7	-0.062180734899953904
2212	8	-0.03811228801468758
2212	9	0.06201069398411363
2212	10	-0.24582469091904358
2212	11	-0.440212211307049
2212	12	0.03661204369550575
2212	13	0.18110500874490043
2212	14	0.21137637477701787
2212	15	-0.02665990086556036
2212	16	-0.17156015265825886
2212	17	0.14302380443665186
2212	18	0.20632937708283094
2212	19	-0.0937826016018235
2212	20	0.12064621139366238
2212	21	0.23346320084166905
2212	22	0.3237933605469365
2212	23	0.3249990839754773
2212	24	0.2565003982597318
2212	25	0.40191564260000945
2213	1	-0.33061637813557354
2213	2	-0.2098884679613775
2213	3	-0.4338798364067351
2213	4	-0.3188008043355097
2213	5	-0.18620804747015995
2213	6	-0.03175070517531964
2213	7	-0.40213481842742027
2213	8	-0.20907687155903432
2213	9	-0.5919757674089112
2213	10	-0.11723620042307914
2213	11	-0.21772875474201214
2213	12	-0.10280142401103376
2213	13	-0.30491874290100185
2213	14	-0.2042272073830773
2213	15	-0.23414453910452693
2213	16	-0.25442925694942176
2213	17	-0.20470442744153416
2213	18	-0.2677991383970948
2213	19	-0.2754181227200263
2213	20	-0.20816468701614355
2213	21	-0.32693848388772295
2213	22	-0.3863610167109073
2213	23	-0.1188802671167295
2213	24	0.03189457091917802
2213	25	0.27235353719469657
2214	1	-2.258717928243488
2214	2	-1.0670701792070787
2214	3	-1.2663089223319446
2214	4	-3.040164156377166
2214	5	-1.3511206577613741
2214	6	-1.184942612420759
2214	7	-1.011161565847999
2214	8	-0.8588946628954233
2214	9	-0.9173782382736704
2214	10	-0.7825939250243437
2214	11	-0.7707428297475047
2214	12	-0.7920168486628967
2214	13	-1.1287668361679266
2214	14	-1.137985018231916
2214	15	-1.4265699104067195
2214	16	-1.1516625032524104
2214	17	-3.221490680774558
2214	18	-2.925879617626162
2214	19	-1.9192997756845713
2214	20	-1.8471003407791216
2214	21	-1.567969056943042
2214	22	-2.6277017821367323
2214	23	-1.558224742895895
2214	24	-2.5704230122702327
2214	25	-2.669781815982695
2215	1	-1.8095914815136922
2215	2	-3.584434330854908
2215	3	-2.0721497226577767
2215	4	-3.5748684889298588
2215	5	-2.3494492817983037
2215	6	-2.003610305798894
2215	7	-2.405920670487717
2215	8	-1.7638771833447997
2215	9	-4.266562172360494
2215	10	-2.636384272239493
2215	11	-2.0748539182313657
2215	12	-1.9645856213608575
2215	13	-2.050114798018381
2215	14	-1.8516570390716112
2215	15	-2.1428233469203093
2215	16	-2.156362890178386
2215	17	-2.2681275643234144
2215	18	-2.1019536341893073
2215	19	-2.1878043407322565
2215	20	-2.032403305514549
2215	21	-2.5763596655029346
2215	22	-2.5390287006446925
2215	23	-2.381200593963335
2215	24	-2.4037149801621673
2215	25	-3.3715344379152903
2216	1	-2.858914082065919
2216	2	-3.025070027054216
2216	3	-4.132650447931663
2216	4	-4.885694871721782
2216	5	-4.5205451292872
2216	6	-3.5296418329457673
2216	7	-5.32318616526447
2216	8	-3.082647780486969
2216	9	-3.9290787932203877
2216	10	-2.8436074275626204
2216	11	-3.017638397370199
2216	12	-3.3736166331815483
2216	13	-3.0465154673062145
2216	14	-4.066453023980824
2216	15	-3.533168615462511
2216	16	-3.782443643699782
2216	17	-3.5818904315728446
2216	18	-3.0636194896943927
2216	19	-3.7729126864715887
2216	20	-4.371252139986691
2216	21	-3.860885724466428
2216	22	-3.5481439301238957
2216	23	-3.586053089796188
2216	24	-4.234986611137725
2216	25	-4.178580569151691
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71781.0
3	1.0
4	617.0
5	3747.0
6	4929.0
7	4717.0
8	3968.0
9	3568.0
10	3282.0
11	3224.0
12	2976.0
13	2848.0
14	2873.0
15	2915.0
16	2942.0
17	3181.0
18	3613.0
19	4288.0
20	5593.0
21	7220.0
22	9119.0
23	11030.0
24	12750.0
25	14614.0
26	15485.0
27	17441.0
28	19520.0
29	23200.0
30	27968.0
31	34408.0
32	43048.0
33	54635.0
34	69578.0
35	92790.0
36	124595.0
37	192827.0
38	502202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.92365872997148	17.804407400337315	12.26380628200756	24.008127587683646
2	16.04921908736046	19.872192270254633	40.16615859958756	23.912430042797347
3	19.95859965654104	25.38211590671028	28.195344064644388	26.463940372104293
4	12.896711440481704	16.190116506929847	36.78241351052838	34.13075854206007
5	14.02305370361397	38.27732852952829	33.30901026984348	14.39060749701425
6	32.534783107863824	34.868465621218355	18.315050704789805	14.281700566128016
7	29.308493266341408	31.544584193194602	20.786061761689954	18.360860778774036
8	26.838952732003467	34.13656308485872	20.520624115579494	18.503860067558314
9	28.441357474452865	14.291847023839235	19.17628769672897	38.09050780497893
10	17.411455451023798	27.67929985908181	31.748384004929285	23.16086068496511
11	35.28326133123769	22.42971059864192	23.054552819206517	19.232475250913872
12	24.876866262425704	24.372206935998985	29.65173086167228	21.099195939903034
13	30.003629707120428	21.31080205117673	25.097820250342824	23.587747991360015
14	22.526509011530678	22.166161885928346	26.517426523916054	28.789902578624922
15	24.979640445271663	28.952784374130886	23.206540729806246	22.861034450791205
16	23.354109022449155	27.333556778959977	26.0364549162792	23.275879282311664
17	22.699107344084766	27.5537262464803	26.222088455382895	23.525077954052044
18	22.637967544096764	26.108650889709413	28.494736774536815	22.75864479165701
19	24.24956691725677	26.24285297580799	27.06494351203182	22.442636594903423
20	25.290118015558544	25.33005559882127	27.131811140874117	22.24801524474607
21	25.18231909460484	25.49393909789931	26.28468376987469	23.039058037621164
22	25.011072874132985	26.610987286700073	25.983060820569513	22.394879018597436
23	23.809004205633137	26.39186345135779	26.997996672293674	22.8011356707154
24	24.231413540129665	25.72782957274088	27.04681229795388	22.99394458917557
25	23.863817891373802	26.208281036056597	26.999515061615703	22.9283860109539
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	261.0
1	261.0
2	594.5
3	928.0
4	928.0
5	928.0
6	1606.5
7	2285.0
8	2285.0
9	2285.0
10	2467.0
11	2649.0
12	2649.0
13	2649.0
14	3203.5
15	3758.0
16	3758.0
17	3758.0
18	7278.5
19	10799.0
20	10799.0
21	10799.0
22	17934.5
23	25070.0
24	25070.0
25	25070.0
26	38967.5
27	52865.0
28	52865.0
29	52865.0
30	68125.0
31	83385.0
32	83385.0
33	83385.0
34	95891.5
35	108398.0
36	108398.0
37	108398.0
38	118884.5
39	129371.0
40	129371.0
41	129371.0
42	143026.0
43	156681.0
44	156681.0
45	156681.0
46	177272.5
47	197864.0
48	197864.0
49	197864.0
50	195644.0
51	193424.0
52	193424.0
53	193424.0
54	174424.5
55	155425.0
56	155425.0
57	155425.0
58	136496.0
59	117567.0
60	117567.0
61	117567.0
62	99859.0
63	82151.0
64	82151.0
65	82151.0
66	62864.5
67	43578.0
68	43578.0
69	43578.0
70	32932.5
71	22287.0
72	22287.0
73	22287.0
74	15852.0
75	9417.0
76	9417.0
77	9417.0
78	6528.0
79	3639.0
80	3639.0
81	3639.0
82	2414.5
83	1190.0
84	1190.0
85	1190.0
86	787.0
87	384.0
88	384.0
89	384.0
90	243.0
91	102.0
92	102.0
93	102.0
94	57.5
95	13.0
96	13.0
97	13.0
98	7.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3420038432681887
2	0.009618858091917808
3	0.008763848483747335
4	0.010188864497364789
5	0.011471378909620498
6	0.08265092878981228
7	0.08086965877279045
8	0.10295740698386098
9	0.08428969720547234
10	0.08984725965858043
11	0.08685472602998376
12	0.09682983812530593
13	0.08379094160070624
14	0.08792348804019685
15	0.0857147132190898
16	0.08642722122589852
17	0.09868235894300863
18	0.10017862575730693
19	0.09519106970964586
20	0.0929822948885388
21	0.08151091597891831
22	0.10103363536547742
23	0.09447856170283714
24	0.0909160216687935
25	0.08927725325313343
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1403493.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.398150776290144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.40821630616365	37.7304656992272
2	14.277136220722774	14.676368002526846
3	5.2362592706969	8.07402130497079
4	2.4963571840469134	5.132325717484733
5	1.3733663860325924	3.529424629019594
6	0.8023392063257062	2.4743250900274565
7	0.5354529976182374	1.9264905733639082
8	0.36085663091339254	1.4837890819448505
9	0.26592479603675656	1.2301238485666275
>10	1.113716148349349	9.717423463495496
>50	0.06399795057249621	2.2724006440718374
>100	0.05599240727340621	5.614282748837392
>500	0.006314895758885465	2.2376850377886
>1k	0.0039292684721954005	3.43190084396014
>5k	1.4033101686412145E-4	0.46897331471446224
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6502	0.46327270602703396	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3935	0.2803719006792339	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3686	0.2626304513096966	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3670	0.2614904384988026	No Hit
GGATACCACGTGTCCCGCCCTACTC	2366	0.1685793944109447	No Hit
GTCCTACAGTGGACATTTCTAAATT	1943	0.13844030572293556	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1913	0.13630278170250937	No Hit
ATCATTAACTGAATCCATAGGTTAA	1883	0.1341652576820832	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1689	0.1203426023499939	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1678	0.1195588435425043	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	1625	0.11578255110641807	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1585	0.11293251907918316	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	1559	0.11107999826148046	No Hit
CCCATGTACTCTGCGTTGATACCAC	1457	0.10381241659203144	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.1375240204261794E-4
13	0.0	0.0	0.0	7.125080068087265E-5	2.1375240204261794E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAGG	130	1.891749E-10	12.456074	1
TAGGACC	390	0.0	11.934306	4
GTCTTAT	80	2.7891268E-5	11.906542	1
ACCGTGT	65	8.031539E-4	11.690749	8
GTATAAT	255	0.0	11.579696	1
ATAATGG	250	0.0	11.39848	3
ATTTAGA	280	0.0	11.226169	1
GGACCGT	110	4.9748087E-7	11.225776	6
TAAGACC	110	4.9748087E-7	11.225776	4
GTGTTAC	85	5.1798714E-5	11.206157	1
GTAAGAC	85	5.3289943E-5	11.174981	3
GTTAGAC	60	0.005881003	11.081856	3
GTCCTAC	945	0.0	10.986777	1
GTATAGA	165	7.6397555E-11	10.968451	1
GTGCAAG	105	3.3564102E-6	10.885981	1
AAGACCG	70	0.0014929035	10.855696	5
GTATAAA	185	5.456968E-12	10.8124275	1
GGTATCA	1860	0.0	10.754295	1
CTAGGAC	170	1.382432E-10	10.616232	3
TCCTACA	1025	0.0	10.564445	2
>>END_MODULE